Role of miR-647 in human gastric cancer suppression
MicroRNAs (miRNAs) regulate various oncogenes concomitantly, resulting in tumor suppression. They regulate proliferation and migration pathways in tumor development, suggesting a potential therapeutic role. In the present study, we found that miR-647 was markedly downregulated in gastric cancer (GC)...
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| Veröffentlicht in: | Oncology reports 2017-03, Vol.37 (3), p.1401-1411 |
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| creator | Cao, Wenlong Wei, Weiyuan Zhan, Zexu Xie, Dongyi Xie, Yubo Xiao, Qiang |
| description | MicroRNAs (miRNAs) regulate various oncogenes concomitantly, resulting in tumor suppression. They regulate proliferation and migration pathways in tumor development, suggesting a potential therapeutic role. In the present study, we found that miR-647 was markedly downregulated in gastric cancer (GC), and was significantly correlated with reduced tumor size and metastasis. In addition, miR-647 was also reduced in GC cell lines. Furthermore, overexpression of miR-647 in the GC cell lines inhibited cell proliferation, promoted cell cycle arrest at the G0/G1 phase and induced cell apoptosis. miR-647 also significantly inhibited tumor growth in vivo. Notably, we found that miR-647 overexpression suppressed the migration and invasion of the cancer cells, particularly liver metastasis in nude mice. miR-647 also reduced the expression levels of genes associated with proliferation and metastasis in tumors, including ANK2, FAK, MMP2, MMP12, CD44 and SNAIL1. Overall, our findings demonstrated that miR-647 exerts powerful antitumorigenic effects in vitro and in vivo, and may represent a promising therapeutic agent against GC. |
| doi_str_mv | 10.3892/or.2017.5383 |
| format | Article |
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They regulate proliferation and migration pathways in tumor development, suggesting a potential therapeutic role. In the present study, we found that miR-647 was markedly downregulated in gastric cancer (GC), and was significantly correlated with reduced tumor size and metastasis. In addition, miR-647 was also reduced in GC cell lines. Furthermore, overexpression of miR-647 in the GC cell lines inhibited cell proliferation, promoted cell cycle arrest at the G0/G1 phase and induced cell apoptosis. miR-647 also significantly inhibited tumor growth in vivo. Notably, we found that miR-647 overexpression suppressed the migration and invasion of the cancer cells, particularly liver metastasis in nude mice. miR-647 also reduced the expression levels of genes associated with proliferation and metastasis in tumors, including ANK2, FAK, MMP2, MMP12, CD44 and SNAIL1. Overall, our findings demonstrated that miR-647 exerts powerful antitumorigenic effects in vitro and in vivo, and may represent a promising therapeutic agent against GC.</description><identifier>ISSN: 1021-335X</identifier><identifier>EISSN: 1791-2431</identifier><identifier>DOI: 10.3892/or.2017.5383</identifier><identifier>PMID: 28098914</identifier><language>eng</language><publisher>Greece: Spandidos Publications</publisher><subject>Animals ; Apoptosis ; Biomarkers, Tumor - genetics ; Biomarkers, Tumor - metabolism ; Blotting, Western ; Care and treatment ; Cell Cycle ; Cell growth ; Cell Movement ; Cell Proliferation ; Development and progression ; Female ; Flow cytometry ; Gastric cancer ; Gene expression ; Gene Expression Regulation, Neoplastic ; Genetic aspects ; Health aspects ; Humans ; Immunoenzyme Techniques ; Lymphatic Metastasis ; Male ; Medical diagnosis ; Medical prognosis ; Metastasis ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; MicroRNA ; MicroRNAs - genetics ; Middle Aged ; Neoplasm Invasiveness ; Neoplasm Staging ; Polymerase chain reaction ; Prognosis ; Real-Time Polymerase Chain Reaction ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - genetics ; Rodents ; Stomach cancer ; Stomach Neoplasms - genetics ; Stomach Neoplasms - metabolism ; Stomach Neoplasms - pathology ; Tumor Cells, Cultured ; Xenograft Model Antitumor Assays</subject><ispartof>Oncology reports, 2017-03, Vol.37 (3), p.1401-1411</ispartof><rights>COPYRIGHT 2017 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2017</rights><rights>Copyright: © Cao et al. 2017</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c510t-4525733db919c1be97d769df95ab7602e36a6d043908bad95770c3a8406593ff3</citedby><cites>FETCH-LOGICAL-c510t-4525733db919c1be97d769df95ab7602e36a6d043908bad95770c3a8406593ff3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28098914$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cao, Wenlong</creatorcontrib><creatorcontrib>Wei, Weiyuan</creatorcontrib><creatorcontrib>Zhan, Zexu</creatorcontrib><creatorcontrib>Xie, Dongyi</creatorcontrib><creatorcontrib>Xie, Yubo</creatorcontrib><creatorcontrib>Xiao, Qiang</creatorcontrib><title>Role of miR-647 in human gastric cancer suppression</title><title>Oncology reports</title><addtitle>Oncol Rep</addtitle><description>MicroRNAs (miRNAs) regulate various oncogenes concomitantly, resulting in tumor suppression. They regulate proliferation and migration pathways in tumor development, suggesting a potential therapeutic role. In the present study, we found that miR-647 was markedly downregulated in gastric cancer (GC), and was significantly correlated with reduced tumor size and metastasis. In addition, miR-647 was also reduced in GC cell lines. Furthermore, overexpression of miR-647 in the GC cell lines inhibited cell proliferation, promoted cell cycle arrest at the G0/G1 phase and induced cell apoptosis. miR-647 also significantly inhibited tumor growth in vivo. Notably, we found that miR-647 overexpression suppressed the migration and invasion of the cancer cells, particularly liver metastasis in nude mice. miR-647 also reduced the expression levels of genes associated with proliferation and metastasis in tumors, including ANK2, FAK, MMP2, MMP12, CD44 and SNAIL1. Overall, our findings demonstrated that miR-647 exerts powerful antitumorigenic effects in vitro and in vivo, and may represent a promising therapeutic agent against GC.</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>Blotting, Western</subject><subject>Care and treatment</subject><subject>Cell Cycle</subject><subject>Cell growth</subject><subject>Cell Movement</subject><subject>Cell Proliferation</subject><subject>Development and progression</subject><subject>Female</subject><subject>Flow cytometry</subject><subject>Gastric cancer</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Genetic aspects</subject><subject>Health aspects</subject><subject>Humans</subject><subject>Immunoenzyme Techniques</subject><subject>Lymphatic Metastasis</subject><subject>Male</subject><subject>Medical diagnosis</subject><subject>Medical prognosis</subject><subject>Metastasis</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Nude</subject><subject>MicroRNA</subject><subject>MicroRNAs - genetics</subject><subject>Middle Aged</subject><subject>Neoplasm Invasiveness</subject><subject>Neoplasm Staging</subject><subject>Polymerase chain reaction</subject><subject>Prognosis</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - genetics</subject><subject>Rodents</subject><subject>Stomach cancer</subject><subject>Stomach Neoplasms - genetics</subject><subject>Stomach Neoplasms - metabolism</subject><subject>Stomach Neoplasms - pathology</subject><subject>Tumor Cells, Cultured</subject><subject>Xenograft Model Antitumor Assays</subject><issn>1021-335X</issn><issn>1791-2431</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNptks1rFTEUxYMotlZ3rmVAEBfOMzc3H5ONUIpfUBCKgruQyWTeS5lJnsmM4H9vHq21TySLhOR3z01ODiHPgW6w0-xtyhtGQW0EdviAnILS0DKO8LCuKYMWUXw_IU9KuaaUKSr1Y3LCOqo7DfyU4FWafJPGZg5XreSqCbHZrbONzdaWJQfXOBudz01Z9_vsSwkpPiWPRjsV_-x2PiPfPrz_evGpvfzy8fPF-WXrBNCl5YIJhTj0GrSD3ms1KKmHUQvbK0mZR2nlQDlq2vV20EIp6tB2nEqhcRzxjLy70d2v_ewH5-OS7WT2Ocw2_zLJBnN8EsPObNNPI1DyTvEq8PpWIKcfqy-LmUNxfpps9GktBjoJQoEAqOjLf9DrtOZYn2dAc2D1jlz9pbZ28ibEMdW-7iBqzrmuMlqiqNTmP1Qdg5-DS9GPoe4fFby6V7Dzdlp2JU3rUs0ux-CbG9DlVEr2450ZQM0hDSZlc0iDOaSh4i_uG3gH__l-_A29aqth</recordid><startdate>20170301</startdate><enddate>20170301</enddate><creator>Cao, Wenlong</creator><creator>Wei, Weiyuan</creator><creator>Zhan, Zexu</creator><creator>Xie, Dongyi</creator><creator>Xie, Yubo</creator><creator>Xiao, Qiang</creator><general>Spandidos Publications</general><general>Spandidos Publications UK Ltd</general><general>D.A. Spandidos</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20170301</creationdate><title>Role of miR-647 in human gastric cancer suppression</title><author>Cao, Wenlong ; Wei, Weiyuan ; Zhan, Zexu ; Xie, Dongyi ; Xie, Yubo ; Xiao, Qiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c510t-4525733db919c1be97d769df95ab7602e36a6d043908bad95770c3a8406593ff3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>Biomarkers, Tumor - genetics</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>Blotting, Western</topic><topic>Care and treatment</topic><topic>Cell Cycle</topic><topic>Cell growth</topic><topic>Cell Movement</topic><topic>Cell Proliferation</topic><topic>Development and progression</topic><topic>Female</topic><topic>Flow cytometry</topic><topic>Gastric cancer</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Genetic aspects</topic><topic>Health aspects</topic><topic>Humans</topic><topic>Immunoenzyme Techniques</topic><topic>Lymphatic Metastasis</topic><topic>Male</topic><topic>Medical diagnosis</topic><topic>Medical prognosis</topic><topic>Metastasis</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Nude</topic><topic>MicroRNA</topic><topic>MicroRNAs - genetics</topic><topic>Middle Aged</topic><topic>Neoplasm Invasiveness</topic><topic>Neoplasm Staging</topic><topic>Polymerase chain reaction</topic><topic>Prognosis</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - genetics</topic><topic>Rodents</topic><topic>Stomach cancer</topic><topic>Stomach Neoplasms - genetics</topic><topic>Stomach Neoplasms - metabolism</topic><topic>Stomach Neoplasms - pathology</topic><topic>Tumor Cells, Cultured</topic><topic>Xenograft Model Antitumor Assays</topic><toplevel>online_resources</toplevel><creatorcontrib>Cao, Wenlong</creatorcontrib><creatorcontrib>Wei, Weiyuan</creatorcontrib><creatorcontrib>Zhan, Zexu</creatorcontrib><creatorcontrib>Xie, Dongyi</creatorcontrib><creatorcontrib>Xie, Yubo</creatorcontrib><creatorcontrib>Xiao, Qiang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Oncology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cao, Wenlong</au><au>Wei, Weiyuan</au><au>Zhan, Zexu</au><au>Xie, Dongyi</au><au>Xie, Yubo</au><au>Xiao, Qiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of miR-647 in human gastric cancer suppression</atitle><jtitle>Oncology reports</jtitle><addtitle>Oncol Rep</addtitle><date>2017-03-01</date><risdate>2017</risdate><volume>37</volume><issue>3</issue><spage>1401</spage><epage>1411</epage><pages>1401-1411</pages><issn>1021-335X</issn><eissn>1791-2431</eissn><abstract>MicroRNAs (miRNAs) regulate various oncogenes concomitantly, resulting in tumor suppression. They regulate proliferation and migration pathways in tumor development, suggesting a potential therapeutic role. In the present study, we found that miR-647 was markedly downregulated in gastric cancer (GC), and was significantly correlated with reduced tumor size and metastasis. In addition, miR-647 was also reduced in GC cell lines. Furthermore, overexpression of miR-647 in the GC cell lines inhibited cell proliferation, promoted cell cycle arrest at the G0/G1 phase and induced cell apoptosis. miR-647 also significantly inhibited tumor growth in vivo. Notably, we found that miR-647 overexpression suppressed the migration and invasion of the cancer cells, particularly liver metastasis in nude mice. miR-647 also reduced the expression levels of genes associated with proliferation and metastasis in tumors, including ANK2, FAK, MMP2, MMP12, CD44 and SNAIL1. Overall, our findings demonstrated that miR-647 exerts powerful antitumorigenic effects in vitro and in vivo, and may represent a promising therapeutic agent against GC.</abstract><cop>Greece</cop><pub>Spandidos Publications</pub><pmid>28098914</pmid><doi>10.3892/or.2017.5383</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Apoptosis Biomarkers, Tumor - genetics Biomarkers, Tumor - metabolism Blotting, Western Care and treatment Cell Cycle Cell growth Cell Movement Cell Proliferation Development and progression Female Flow cytometry Gastric cancer Gene expression Gene Expression Regulation, Neoplastic Genetic aspects Health aspects Humans Immunoenzyme Techniques Lymphatic Metastasis Male Medical diagnosis Medical prognosis Metastasis Mice Mice, Inbred BALB C Mice, Nude MicroRNA MicroRNAs - genetics Middle Aged Neoplasm Invasiveness Neoplasm Staging Polymerase chain reaction Prognosis Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics Rodents Stomach cancer Stomach Neoplasms - genetics Stomach Neoplasms - metabolism Stomach Neoplasms - pathology Tumor Cells, Cultured Xenograft Model Antitumor Assays |
| title | Role of miR-647 in human gastric cancer suppression |
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