Double-flow focused liquid injector for efficient serial femtosecond crystallography
Serial femtosecond crystallography requires reliable and efficient delivery of fresh crystals across the beam of an X-ray free-electron laser over the course of an experiment. We introduce a double-flow focusing nozzle to meet this challenge, with significantly reduced sample consumption, while impr...
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| Veröffentlicht in: | Scientific reports 2017-03, Vol.7 (1), p.44628-44628, Article 44628 |
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| creator | Oberthuer, Dominik Knoška, Juraj Wiedorn, Max O. Beyerlein, Kenneth R. Bushnell, David A. Kovaleva, Elena G. Heymann, Michael Gumprecht, Lars Kirian, Richard A. Barty, Anton Mariani, Valerio Tolstikova, Aleksandra Adriano, Luigi Awel, Salah Barthelmess, Miriam Dörner, Katerina Xavier, P. Lourdu Yefanov, Oleksandr James, Daniel R. Nelson, Garrett Wang, Dingjie Calvey, George Chen, Yujie Schmidt, Andrea Szczepek, Michael Frielingsdorf, Stefan Lenz, Oliver Snell, Edward Robinson, Philip J. Šarler, Božidar Belšak, Grega Maček, Marjan Wilde, Fabian Aquila, Andrew Boutet, Sébastien Liang, Mengning Hunter, Mark S. Scheerer, Patrick Lipscomb, John D. Weierstall, Uwe Kornberg, Roger D. Spence, John C. H. Pollack, Lois Chapman, Henry N. Bajt, Saša |
| description | Serial femtosecond crystallography requires reliable and efficient delivery of fresh crystals across the beam of an X-ray free-electron laser over the course of an experiment. We introduce a double-flow focusing nozzle to meet this challenge, with significantly reduced sample consumption, while improving jet stability over previous generations of nozzles. We demonstrate its use to determine the first room-temperature structure of RNA polymerase II at high resolution, revealing new structural details. Moreover, the double flow-focusing nozzles were successfully tested with three other protein samples and the first room temperature structure of an extradiol ring-cleaving dioxygenase was solved by utilizing the improved operation and characteristics of these devices. |
| doi_str_mv | 10.1038/srep44628 |
| format | Article |
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Lourdu ; Yefanov, Oleksandr ; James, Daniel R. ; Nelson, Garrett ; Wang, Dingjie ; Calvey, George ; Chen, Yujie ; Schmidt, Andrea ; Szczepek, Michael ; Frielingsdorf, Stefan ; Lenz, Oliver ; Snell, Edward ; Robinson, Philip J. ; Šarler, Božidar ; Belšak, Grega ; Maček, Marjan ; Wilde, Fabian ; Aquila, Andrew ; Boutet, Sébastien ; Liang, Mengning ; Hunter, Mark S. ; Scheerer, Patrick ; Lipscomb, John D. ; Weierstall, Uwe ; Kornberg, Roger D. ; Spence, John C. H. ; Pollack, Lois ; Chapman, Henry N. ; Bajt, Saša ; SLAC National Accelerator Lab., Menlo Park, CA (United States)</creatorcontrib><description>Serial femtosecond crystallography requires reliable and efficient delivery of fresh crystals across the beam of an X-ray free-electron laser over the course of an experiment. We introduce a double-flow focusing nozzle to meet this challenge, with significantly reduced sample consumption, while improving jet stability over previous generations of nozzles. We demonstrate its use to determine the first room-temperature structure of RNA polymerase II at high resolution, revealing new structural details. 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Lourdu</creatorcontrib><creatorcontrib>Yefanov, Oleksandr</creatorcontrib><creatorcontrib>James, Daniel R.</creatorcontrib><creatorcontrib>Nelson, Garrett</creatorcontrib><creatorcontrib>Wang, Dingjie</creatorcontrib><creatorcontrib>Calvey, George</creatorcontrib><creatorcontrib>Chen, Yujie</creatorcontrib><creatorcontrib>Schmidt, Andrea</creatorcontrib><creatorcontrib>Szczepek, Michael</creatorcontrib><creatorcontrib>Frielingsdorf, Stefan</creatorcontrib><creatorcontrib>Lenz, Oliver</creatorcontrib><creatorcontrib>Snell, Edward</creatorcontrib><creatorcontrib>Robinson, Philip J.</creatorcontrib><creatorcontrib>Šarler, Božidar</creatorcontrib><creatorcontrib>Belšak, Grega</creatorcontrib><creatorcontrib>Maček, Marjan</creatorcontrib><creatorcontrib>Wilde, Fabian</creatorcontrib><creatorcontrib>Aquila, Andrew</creatorcontrib><creatorcontrib>Boutet, Sébastien</creatorcontrib><creatorcontrib>Liang, Mengning</creatorcontrib><creatorcontrib>Hunter, Mark S.</creatorcontrib><creatorcontrib>Scheerer, Patrick</creatorcontrib><creatorcontrib>Lipscomb, John D.</creatorcontrib><creatorcontrib>Weierstall, Uwe</creatorcontrib><creatorcontrib>Kornberg, Roger D.</creatorcontrib><creatorcontrib>Spence, John C. 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H.</creatorcontrib><creatorcontrib>Pollack, Lois</creatorcontrib><creatorcontrib>Chapman, Henry N.</creatorcontrib><creatorcontrib>Bajt, Saša</creatorcontrib><creatorcontrib>SLAC National Accelerator Lab., Menlo Park, CA (United States)</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV - Hybrid</collection><collection>OSTI.GOV</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oberthuer, Dominik</au><au>Knoška, Juraj</au><au>Wiedorn, Max O.</au><au>Beyerlein, Kenneth R.</au><au>Bushnell, David A.</au><au>Kovaleva, Elena G.</au><au>Heymann, Michael</au><au>Gumprecht, Lars</au><au>Kirian, Richard A.</au><au>Barty, Anton</au><au>Mariani, Valerio</au><au>Tolstikova, Aleksandra</au><au>Adriano, Luigi</au><au>Awel, Salah</au><au>Barthelmess, Miriam</au><au>Dörner, Katerina</au><au>Xavier, P. Lourdu</au><au>Yefanov, Oleksandr</au><au>James, Daniel R.</au><au>Nelson, Garrett</au><au>Wang, Dingjie</au><au>Calvey, George</au><au>Chen, Yujie</au><au>Schmidt, Andrea</au><au>Szczepek, Michael</au><au>Frielingsdorf, Stefan</au><au>Lenz, Oliver</au><au>Snell, Edward</au><au>Robinson, Philip J.</au><au>Šarler, Božidar</au><au>Belšak, Grega</au><au>Maček, Marjan</au><au>Wilde, Fabian</au><au>Aquila, Andrew</au><au>Boutet, Sébastien</au><au>Liang, Mengning</au><au>Hunter, Mark S.</au><au>Scheerer, Patrick</au><au>Lipscomb, John D.</au><au>Weierstall, Uwe</au><au>Kornberg, Roger D.</au><au>Spence, John C. H.</au><au>Pollack, Lois</au><au>Chapman, Henry N.</au><au>Bajt, Saša</au><aucorp>SLAC National Accelerator Lab., Menlo Park, CA (United States)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Double-flow focused liquid injector for efficient serial femtosecond crystallography</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2017-03-16</date><risdate>2017</risdate><volume>7</volume><issue>1</issue><spage>44628</spage><epage>44628</epage><pages>44628-44628</pages><artnum>44628</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Serial femtosecond crystallography requires reliable and efficient delivery of fresh crystals across the beam of an X-ray free-electron laser over the course of an experiment. We introduce a double-flow focusing nozzle to meet this challenge, with significantly reduced sample consumption, while improving jet stability over previous generations of nozzles. We demonstrate its use to determine the first room-temperature structure of RNA polymerase II at high resolution, revealing new structural details. Moreover, the double flow-focusing nozzles were successfully tested with three other protein samples and the first room temperature structure of an extradiol ring-cleaving dioxygenase was solved by utilizing the improved operation and characteristics of these devices.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>28300169</pmid><doi>10.1038/srep44628</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2045-2322 |
| ispartof | Scientific reports, 2017-03, Vol.7 (1), p.44628-44628, Article 44628 |
| issn | 2045-2322 2045-2322 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5353652 |
| source | MEDLINE; Nature Free; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry; Springer Nature OA Free Journals |
| subjects | 631/1647/2258/1266/1265 639/766/189 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS Computer Simulation Crystallography Crystallography - instrumentation Crystals Dioxygenase DNA-directed RNA polymerase fluid dynamics Humanities and Social Sciences multidisciplinary nanocrystallography Nozzles Rheology - instrumentation RNA Polymerase II - chemistry Saccharomyces cerevisiae - enzymology Science Temperature Temperature effects Time Factors X-Ray Diffraction |
| title | Double-flow focused liquid injector for efficient serial femtosecond crystallography |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-31T20%3A05%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Double-flow%20focused%20liquid%20injector%20for%20efficient%20serial%20femtosecond%20crystallography&rft.jtitle=Scientific%20reports&rft.au=Oberthuer,%20Dominik&rft.aucorp=SLAC%20National%20Accelerator%20Lab.,%20Menlo%20Park,%20CA%20(United%20States)&rft.date=2017-03-16&rft.volume=7&rft.issue=1&rft.spage=44628&rft.epage=44628&rft.pages=44628-44628&rft.artnum=44628&rft.issn=2045-2322&rft.eissn=2045-2322&rft_id=info:doi/10.1038/srep44628&rft_dat=%3Cproquest_pubme%3E1903385183%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1903385183&rft_id=info:pmid/28300169&rfr_iscdi=true |