ThermoAlign: a genome-aware primer design tool for tiled amplicon resequencing

Isolating and sequencing specific regions in a genome is a cornerstone of molecular biology. This has been facilitated by computationally encoding the thermodynamics of DNA hybridization for automated design of hybridization and priming oligonucleotides. However, the repetitive composition of genome...

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Veröffentlicht in:	Scientific reports 2017-03, Vol.7 (1), p.44437-44437, Article 44437
Hauptverfasser:	Francis, Felix, Dumas, Michael D., Wisser, Randall J.
Format:	Artikel
Sprache:	eng
Schlagworte:	45/22 45/56 631/114/794 631/208/514 Base Sequence Deoxyribonucleic acid Design DNA DNA Primers - chemical synthesis DNA Primers - metabolism Genetics Genome, Plant Genomes Genomics High-Throughput Nucleotide Sequencing Humanities and Social Sciences Hybridization Microsatellite Repeats Molecular biology multidisciplinary Nucleic Acid Hybridization - methods Oligonucleotides Polymerase chain reaction Polymerase Chain Reaction - methods Polymorphism, Single Nucleotide Primers Science Sequence Alignment Sequence Analysis, DNA - methods Software Thermodynamics Zea mays - genetics
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