Biogenic silver nanoparticles based on trichoderma harzianum: synthesis, characterization, toxicity evaluation and biological activity
White mold is an agricultural disease caused by the fungus Sclerotinia sclerotiorum , which affects important crops. There are different ways of controlling this organism, but none provides inhibition of its resistance structures (sclerotia). Nanotechnology offers promising applications in agricultu...
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| Veröffentlicht in: | Scientific reports 2017-03, Vol.7 (1), p.44421, Article 44421 |
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| description | White mold is an agricultural disease caused by the fungus
Sclerotinia sclerotiorum
, which affects important crops. There are different ways of controlling this organism, but none provides inhibition of its resistance structures (sclerotia). Nanotechnology offers promising applications in agricultural area. Here, silver nanoparticles were biogenically synthesized using the fungus
Trichoderma harzianum
and characterized. Cytotoxicity and genotoxicity were evaluated, and the nanoparticles were initially tested against white mold sclerotia. Their effects on soybean were also investigated with no effects observed. The nanoparticles showed potential against
S. sclerotiorum
, inhibiting sclerotia germination and mycelial growth. Nanoparticle characterization data indicated spherical morphology, satisfactory polydispersity and size distribution. Cytotoxicity and genotoxicity assays showed that the nanoparticles caused both the effects, although, the most toxic concentrations were above those applied for white mold control. Given the potential of the nanoparticles against
S. sclerotiorum
, we conclude that this study presents a first step for a new alternative in white mold control. |
| doi_str_mv | 10.1038/srep44421 |
| format | Article |
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Sclerotinia sclerotiorum
, which affects important crops. There are different ways of controlling this organism, but none provides inhibition of its resistance structures (sclerotia). Nanotechnology offers promising applications in agricultural area. Here, silver nanoparticles were biogenically synthesized using the fungus
Trichoderma harzianum
and characterized. Cytotoxicity and genotoxicity were evaluated, and the nanoparticles were initially tested against white mold sclerotia. Their effects on soybean were also investigated with no effects observed. The nanoparticles showed potential against
S. sclerotiorum
, inhibiting sclerotia germination and mycelial growth. Nanoparticle characterization data indicated spherical morphology, satisfactory polydispersity and size distribution. Cytotoxicity and genotoxicity assays showed that the nanoparticles caused both the effects, although, the most toxic concentrations were above those applied for white mold control. Given the potential of the nanoparticles against
S. sclerotiorum
, we conclude that this study presents a first step for a new alternative in white mold control.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep44421</identifier><identifier>PMID: 28300141</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>704/158 ; 704/172/169 ; A549 Cells ; Animals ; Antifungal Agents - chemistry ; Antifungal Agents - pharmacology ; Ascomycota - drug effects ; Ascomycota - growth & development ; Beans ; Biological activity ; Cell Survival - drug effects ; Chromosome Aberrations - drug effects ; Cytotoxicity ; Genotoxicity ; Germination ; Glycine max - drug effects ; Glycine max - microbiology ; HeLa Cells ; Humanities and Social Sciences ; Humans ; Metal Nanoparticles - chemistry ; Metal Nanoparticles - toxicity ; Mice ; Microbial Sensitivity Tests ; Mitotic Index ; Mold ; multidisciplinary ; Mycelia ; Mycelium - drug effects ; Mycelium - growth & development ; Nanoparticles ; Nanotechnology ; NIH 3T3 Cells ; Onions - cytology ; Onions - drug effects ; Oxidation-Reduction ; Particle Size ; Plant Diseases - microbiology ; Plant Diseases - therapy ; Science ; Sclerotia ; Silver ; Silver - chemistry ; Silver - pharmacology ; Size distribution ; Soybeans ; Toxicity ; Trichoderma - chemistry ; Trichoderma - metabolism ; White mold</subject><ispartof>Scientific reports, 2017-03, Vol.7 (1), p.44421, Article 44421</ispartof><rights>The Author(s) 2017</rights><rights>Copyright Nature Publishing Group Mar 2017</rights><rights>Copyright © 2017, The Author(s) 2017 The Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-b0431eac63776816d9cf828972976b378c0e11d4cfc4a28795eea24e10c4a38a3</citedby><cites>FETCH-LOGICAL-c438t-b0431eac63776816d9cf828972976b378c0e11d4cfc4a28795eea24e10c4a38a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5353535/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5353535/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27903,27904,41099,42168,51555,53770,53772</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28300141$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guilger, Mariana</creatorcontrib><creatorcontrib>Pasquoto-Stigliani, Tatiane</creatorcontrib><creatorcontrib>Bilesky-Jose, Natália</creatorcontrib><creatorcontrib>Grillo, Renato</creatorcontrib><creatorcontrib>Abhilash, P. C.</creatorcontrib><creatorcontrib>Fraceto, Leonardo Fernandes</creatorcontrib><creatorcontrib>Lima, Renata de</creatorcontrib><title>Biogenic silver nanoparticles based on trichoderma harzianum: synthesis, characterization, toxicity evaluation and biological activity</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>White mold is an agricultural disease caused by the fungus
Sclerotinia sclerotiorum
, which affects important crops. There are different ways of controlling this organism, but none provides inhibition of its resistance structures (sclerotia). Nanotechnology offers promising applications in agricultural area. Here, silver nanoparticles were biogenically synthesized using the fungus
Trichoderma harzianum
and characterized. Cytotoxicity and genotoxicity were evaluated, and the nanoparticles were initially tested against white mold sclerotia. Their effects on soybean were also investigated with no effects observed. The nanoparticles showed potential against
S. sclerotiorum
, inhibiting sclerotia germination and mycelial growth. Nanoparticle characterization data indicated spherical morphology, satisfactory polydispersity and size distribution. Cytotoxicity and genotoxicity assays showed that the nanoparticles caused both the effects, although, the most toxic concentrations were above those applied for white mold control. Given the potential of the nanoparticles against
S. sclerotiorum
, we conclude that this study presents a first step for a new alternative in white mold control.</description><subject>704/158</subject><subject>704/172/169</subject><subject>A549 Cells</subject><subject>Animals</subject><subject>Antifungal Agents - chemistry</subject><subject>Antifungal Agents - pharmacology</subject><subject>Ascomycota - drug effects</subject><subject>Ascomycota - growth & development</subject><subject>Beans</subject><subject>Biological activity</subject><subject>Cell Survival - drug effects</subject><subject>Chromosome Aberrations - drug effects</subject><subject>Cytotoxicity</subject><subject>Genotoxicity</subject><subject>Germination</subject><subject>Glycine max - drug effects</subject><subject>Glycine max - microbiology</subject><subject>HeLa Cells</subject><subject>Humanities and Social Sciences</subject><subject>Humans</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Metal Nanoparticles - toxicity</subject><subject>Mice</subject><subject>Microbial Sensitivity Tests</subject><subject>Mitotic Index</subject><subject>Mold</subject><subject>multidisciplinary</subject><subject>Mycelia</subject><subject>Mycelium - drug effects</subject><subject>Mycelium - growth & development</subject><subject>Nanoparticles</subject><subject>Nanotechnology</subject><subject>NIH 3T3 Cells</subject><subject>Onions - cytology</subject><subject>Onions - drug effects</subject><subject>Oxidation-Reduction</subject><subject>Particle Size</subject><subject>Plant Diseases - microbiology</subject><subject>Plant Diseases - therapy</subject><subject>Science</subject><subject>Sclerotia</subject><subject>Silver</subject><subject>Silver - chemistry</subject><subject>Silver - pharmacology</subject><subject>Size distribution</subject><subject>Soybeans</subject><subject>Toxicity</subject><subject>Trichoderma - chemistry</subject><subject>Trichoderma - metabolism</subject><subject>White mold</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNplkd1uEzEQhS0EolXoBS-ALHEFasB_2fVyUQkq_qRK3MC1NeudJFNt7GB7I9IH4LkxpERB2Be2Zz6fOdJh7KkUr6TQ9nVOuDXGKPmAnSthFnOllXp4cj9jFznfiroWqjOye8zOlNVCSCPP2c93FFcYyPNM4w4TDxDiFlIhP2LmPWQceAy8JPLrOGDaAF9DuiMI0-YNz_tQ1pgpX3Jfy-ALJrqDQjFc8hJ_kKey57iDcfpT5BAG3lMc44o8jLx-oF1FnrBHSxgzXtyfM_btw_uv15_mN18-fr5-ezP3Rtsy74XREsE3um0bK5uh80urbNeqrm163VovUMrB-KU3oGzbLRBBGZSivrUFPWNXB93t1G9w8BhKgtFtE20g7V0Ecv92Aq3dKu7cQh_2jD2_F0jx-4S5uNs4pVA9O9kJXS3ItqnUiwPlU8w1oOVxghTud2rumFpln51aOpJ_M6rAywOQayusMJ2M_E_tF-zPpUI</recordid><startdate>20170316</startdate><enddate>20170316</enddate><creator>Guilger, Mariana</creator><creator>Pasquoto-Stigliani, Tatiane</creator><creator>Bilesky-Jose, Natália</creator><creator>Grillo, Renato</creator><creator>Abhilash, P. C.</creator><creator>Fraceto, Leonardo Fernandes</creator><creator>Lima, Renata de</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>5PM</scope></search><sort><creationdate>20170316</creationdate><title>Biogenic silver nanoparticles based on trichoderma harzianum: synthesis, characterization, toxicity evaluation and biological activity</title><author>Guilger, Mariana ; Pasquoto-Stigliani, Tatiane ; Bilesky-Jose, Natália ; Grillo, Renato ; Abhilash, P. C. ; Fraceto, Leonardo Fernandes ; Lima, Renata de</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-b0431eac63776816d9cf828972976b378c0e11d4cfc4a28795eea24e10c4a38a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>704/158</topic><topic>704/172/169</topic><topic>A549 Cells</topic><topic>Animals</topic><topic>Antifungal Agents - chemistry</topic><topic>Antifungal Agents - pharmacology</topic><topic>Ascomycota - drug effects</topic><topic>Ascomycota - growth & development</topic><topic>Beans</topic><topic>Biological activity</topic><topic>Cell Survival - drug effects</topic><topic>Chromosome Aberrations - drug effects</topic><topic>Cytotoxicity</topic><topic>Genotoxicity</topic><topic>Germination</topic><topic>Glycine max - drug effects</topic><topic>Glycine max - microbiology</topic><topic>HeLa Cells</topic><topic>Humanities and Social Sciences</topic><topic>Humans</topic><topic>Metal Nanoparticles - chemistry</topic><topic>Metal Nanoparticles - toxicity</topic><topic>Mice</topic><topic>Microbial Sensitivity Tests</topic><topic>Mitotic Index</topic><topic>Mold</topic><topic>multidisciplinary</topic><topic>Mycelia</topic><topic>Mycelium - drug effects</topic><topic>Mycelium - growth & development</topic><topic>Nanoparticles</topic><topic>Nanotechnology</topic><topic>NIH 3T3 Cells</topic><topic>Onions - cytology</topic><topic>Onions - drug effects</topic><topic>Oxidation-Reduction</topic><topic>Particle Size</topic><topic>Plant Diseases - microbiology</topic><topic>Plant Diseases - therapy</topic><topic>Science</topic><topic>Sclerotia</topic><topic>Silver</topic><topic>Silver - chemistry</topic><topic>Silver - pharmacology</topic><topic>Size distribution</topic><topic>Soybeans</topic><topic>Toxicity</topic><topic>Trichoderma - chemistry</topic><topic>Trichoderma - metabolism</topic><topic>White mold</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guilger, Mariana</creatorcontrib><creatorcontrib>Pasquoto-Stigliani, Tatiane</creatorcontrib><creatorcontrib>Bilesky-Jose, Natália</creatorcontrib><creatorcontrib>Grillo, Renato</creatorcontrib><creatorcontrib>Abhilash, P. 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C.</au><au>Fraceto, Leonardo Fernandes</au><au>Lima, Renata de</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biogenic silver nanoparticles based on trichoderma harzianum: synthesis, characterization, toxicity evaluation and biological activity</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2017-03-16</date><risdate>2017</risdate><volume>7</volume><issue>1</issue><spage>44421</spage><pages>44421-</pages><artnum>44421</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>White mold is an agricultural disease caused by the fungus
Sclerotinia sclerotiorum
, which affects important crops. There are different ways of controlling this organism, but none provides inhibition of its resistance structures (sclerotia). Nanotechnology offers promising applications in agricultural area. Here, silver nanoparticles were biogenically synthesized using the fungus
Trichoderma harzianum
and characterized. Cytotoxicity and genotoxicity were evaluated, and the nanoparticles were initially tested against white mold sclerotia. Their effects on soybean were also investigated with no effects observed. The nanoparticles showed potential against
S. sclerotiorum
, inhibiting sclerotia germination and mycelial growth. Nanoparticle characterization data indicated spherical morphology, satisfactory polydispersity and size distribution. Cytotoxicity and genotoxicity assays showed that the nanoparticles caused both the effects, although, the most toxic concentrations were above those applied for white mold control. Given the potential of the nanoparticles against
S. sclerotiorum
, we conclude that this study presents a first step for a new alternative in white mold control.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>28300141</pmid><doi>10.1038/srep44421</doi><oa>free_for_read</oa></addata></record> |
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| subjects | 704/158 704/172/169 A549 Cells Animals Antifungal Agents - chemistry Antifungal Agents - pharmacology Ascomycota - drug effects Ascomycota - growth & development Beans Biological activity Cell Survival - drug effects Chromosome Aberrations - drug effects Cytotoxicity Genotoxicity Germination Glycine max - drug effects Glycine max - microbiology HeLa Cells Humanities and Social Sciences Humans Metal Nanoparticles - chemistry Metal Nanoparticles - toxicity Mice Microbial Sensitivity Tests Mitotic Index Mold multidisciplinary Mycelia Mycelium - drug effects Mycelium - growth & development Nanoparticles Nanotechnology NIH 3T3 Cells Onions - cytology Onions - drug effects Oxidation-Reduction Particle Size Plant Diseases - microbiology Plant Diseases - therapy Science Sclerotia Silver Silver - chemistry Silver - pharmacology Size distribution Soybeans Toxicity Trichoderma - chemistry Trichoderma - metabolism White mold |
| title | Biogenic silver nanoparticles based on trichoderma harzianum: synthesis, characterization, toxicity evaluation and biological activity |
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