SNHG8 is identified as a key regulator of epstein-barr virus(EBV)-associated gastric cancer by an integrative analysis of lncRNA and mRNA expression
The Epstein-Barr virus (EBV) is associated with a variety of cancers, including gastric cancer, which has one of the highest mortality rates of all human cancers. Long non-coding RNAs (lncRNAs) have been suggested to have important causal roles in gastric cancer. However, the interaction between lnc...
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| Veröffentlicht in: | Oncotarget 2016-12, Vol.7 (49), p.80990-81002 |
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| creator | Huang, Tao Ji, Yan Hu, Dan Chen, Baozheng Zhang, Hejun Li, Chao Chen, Gang Luo, Xingguang Zheng, Xiong-Wei Lin, Xiandong |
| description | The Epstein-Barr virus (EBV) is associated with a variety of cancers, including gastric cancer, which has one of the highest mortality rates of all human cancers. Long non-coding RNAs (lncRNAs) have been suggested to have important causal roles in gastric cancer. However, the interaction between lncRNAs and EBV has not yet been studied. To this end, we sequenced 11,311 lncRNAs and 144,826 protein-coding transcripts from four types of tissue: one non-EBV-infected gastric carcinoma (EBVnGC) and its adjacent normal tissue, and one EBV-associated gastric carcinoma (EBVaGC) and its adjacent normal tissue. Five lncRNAs showed EBVaGC-specific expression; of those, one (SNHG8) was validated using real-time PCR in an independent cohort with 88 paired gastric cancer and adjacent tissue samples. To explore the functions of SNHG8, we identified its mRNA targets on the lncRNA-mRNA co-expression network of the Illumina Body Map, which contains the RNA sequencing data of mRNAs and lncRNAs from 16 normal human tissues. SNHG8 lncRNA was found to affect several gastric cancer-specific pathways and target genes of EBV. Our results reveal the intertwined tumorigenesis mechanisms of lncRNA and EBV and identify SNHG8 as a highly possible candidate biomarker and drug target of gastric cancer. |
| doi_str_mv | 10.18632/oncotarget.13167 |
| format | Article |
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Long non-coding RNAs (lncRNAs) have been suggested to have important causal roles in gastric cancer. However, the interaction between lncRNAs and EBV has not yet been studied. To this end, we sequenced 11,311 lncRNAs and 144,826 protein-coding transcripts from four types of tissue: one non-EBV-infected gastric carcinoma (EBVnGC) and its adjacent normal tissue, and one EBV-associated gastric carcinoma (EBVaGC) and its adjacent normal tissue. Five lncRNAs showed EBVaGC-specific expression; of those, one (SNHG8) was validated using real-time PCR in an independent cohort with 88 paired gastric cancer and adjacent tissue samples. To explore the functions of SNHG8, we identified its mRNA targets on the lncRNA-mRNA co-expression network of the Illumina Body Map, which contains the RNA sequencing data of mRNAs and lncRNAs from 16 normal human tissues. SNHG8 lncRNA was found to affect several gastric cancer-specific pathways and target genes of EBV. Our results reveal the intertwined tumorigenesis mechanisms of lncRNA and EBV and identify SNHG8 as a highly possible candidate biomarker and drug target of gastric cancer.</description><identifier>ISSN: 1949-2553</identifier><identifier>EISSN: 1949-2553</identifier><identifier>DOI: 10.18632/oncotarget.13167</identifier><identifier>PMID: 27835598</identifier><language>eng</language><publisher>United States: Impact Journals LLC</publisher><subject>Adult ; Aged ; Cell Transformation, Viral ; Computational Biology ; Databases, Genetic ; Epstein-Barr Virus Infections - virology ; Female ; Gene Expression Profiling ; Gene Expression Regulation, Neoplastic ; Gene Regulatory Networks ; Herpesvirus 4, Human - pathogenicity ; Host-Pathogen Interactions ; Humans ; Male ; Middle Aged ; Real-Time Polymerase Chain Reaction ; Research Paper ; RNA, Long Noncoding - genetics ; RNA, Messenger - genetics ; RNA, Neoplasm - genetics ; Sequence Analysis, RNA ; Stomach Neoplasms - genetics ; Stomach Neoplasms - pathology ; Stomach Neoplasms - virology ; Young Adult</subject><ispartof>Oncotarget, 2016-12, Vol.7 (49), p.80990-81002</ispartof><rights>Copyright: © 2016 Huang et al. 2016</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c337t-448d083c193e83f4fcb52779b6c57de4ed1b9db25292f81a76943ecf8ca39d893</citedby><cites>FETCH-LOGICAL-c337t-448d083c193e83f4fcb52779b6c57de4ed1b9db25292f81a76943ecf8ca39d893</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5348371/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5348371/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27835598$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huang, Tao</creatorcontrib><creatorcontrib>Ji, Yan</creatorcontrib><creatorcontrib>Hu, Dan</creatorcontrib><creatorcontrib>Chen, Baozheng</creatorcontrib><creatorcontrib>Zhang, Hejun</creatorcontrib><creatorcontrib>Li, Chao</creatorcontrib><creatorcontrib>Chen, Gang</creatorcontrib><creatorcontrib>Luo, Xingguang</creatorcontrib><creatorcontrib>Zheng, Xiong-Wei</creatorcontrib><creatorcontrib>Lin, Xiandong</creatorcontrib><title>SNHG8 is identified as a key regulator of epstein-barr virus(EBV)-associated gastric cancer by an integrative analysis of lncRNA and mRNA expression</title><title>Oncotarget</title><addtitle>Oncotarget</addtitle><description>The Epstein-Barr virus (EBV) is associated with a variety of cancers, including gastric cancer, which has one of the highest mortality rates of all human cancers. 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Our results reveal the intertwined tumorigenesis mechanisms of lncRNA and EBV and identify SNHG8 as a highly possible candidate biomarker and drug target of gastric cancer.</description><subject>Adult</subject><subject>Aged</subject><subject>Cell Transformation, Viral</subject><subject>Computational Biology</subject><subject>Databases, Genetic</subject><subject>Epstein-Barr Virus Infections - virology</subject><subject>Female</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Gene Regulatory Networks</subject><subject>Herpesvirus 4, Human - pathogenicity</subject><subject>Host-Pathogen Interactions</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Research Paper</subject><subject>RNA, Long Noncoding - genetics</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Neoplasm - genetics</subject><subject>Sequence Analysis, RNA</subject><subject>Stomach Neoplasms - genetics</subject><subject>Stomach Neoplasms - pathology</subject><subject>Stomach Neoplasms - virology</subject><subject>Young Adult</subject><issn>1949-2553</issn><issn>1949-2553</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVUU1PGzEQtSpQQcAP6KXyEQ4L8Xod2xckiCggoSDRj-tq1p7dut3Yke1E5H_0B2NCS2Eu82ZG7z1pHiGf2OSUqSmvz4I3IUMcMJ8yzqbyA9lnutFVLQTfeYP3yFFKvyalRCNVrT-SvVoqLoRW--TP1_nNtaIuUWfRZ9c7tBQSBfobNzTisBohh0hDT3GZMjpfdRAjXbu4SsdXlz9OKkgpGAe5EAdIOTpDDXiDkXYbCp46n3GIkN0aywjjJhW3ojd68zC_KCtLF88AH5cRU3LBH5LdHsaER3_7Afn-5erb7Ka6u7--nV3cVYZzmaumUXaiuGGao-J905tO1FLqbmqEtNigZZ22XS1qXfeKgZzqhqPplQGurdL8gJy_6C5X3QKtKQ-IMLbL6BYQN20A176_ePezHcK6FbxRXLIiwF4ETAwpRexfuWzSblNq_6fUblMqnM9vTV8Z_zLhT5TwlAQ</recordid><startdate>20161206</startdate><enddate>20161206</enddate><creator>Huang, Tao</creator><creator>Ji, Yan</creator><creator>Hu, Dan</creator><creator>Chen, Baozheng</creator><creator>Zhang, Hejun</creator><creator>Li, Chao</creator><creator>Chen, Gang</creator><creator>Luo, Xingguang</creator><creator>Zheng, Xiong-Wei</creator><creator>Lin, Xiandong</creator><general>Impact Journals LLC</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>20161206</creationdate><title>SNHG8 is identified as a key regulator of epstein-barr virus(EBV)-associated gastric cancer by an integrative analysis of lncRNA and mRNA expression</title><author>Huang, Tao ; Ji, Yan ; Hu, Dan ; Chen, Baozheng ; Zhang, Hejun ; Li, Chao ; Chen, Gang ; Luo, Xingguang ; Zheng, Xiong-Wei ; Lin, Xiandong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c337t-448d083c193e83f4fcb52779b6c57de4ed1b9db25292f81a76943ecf8ca39d893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Cell Transformation, Viral</topic><topic>Computational Biology</topic><topic>Databases, Genetic</topic><topic>Epstein-Barr Virus Infections - virology</topic><topic>Female</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Gene Regulatory Networks</topic><topic>Herpesvirus 4, Human - pathogenicity</topic><topic>Host-Pathogen Interactions</topic><topic>Humans</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Research Paper</topic><topic>RNA, Long Noncoding - genetics</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Neoplasm - genetics</topic><topic>Sequence Analysis, RNA</topic><topic>Stomach Neoplasms - genetics</topic><topic>Stomach Neoplasms - pathology</topic><topic>Stomach Neoplasms - virology</topic><topic>Young Adult</topic><toplevel>online_resources</toplevel><creatorcontrib>Huang, Tao</creatorcontrib><creatorcontrib>Ji, Yan</creatorcontrib><creatorcontrib>Hu, Dan</creatorcontrib><creatorcontrib>Chen, Baozheng</creatorcontrib><creatorcontrib>Zhang, Hejun</creatorcontrib><creatorcontrib>Li, Chao</creatorcontrib><creatorcontrib>Chen, Gang</creatorcontrib><creatorcontrib>Luo, Xingguang</creatorcontrib><creatorcontrib>Zheng, Xiong-Wei</creatorcontrib><creatorcontrib>Lin, Xiandong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Oncotarget</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, Tao</au><au>Ji, Yan</au><au>Hu, Dan</au><au>Chen, Baozheng</au><au>Zhang, Hejun</au><au>Li, Chao</au><au>Chen, Gang</au><au>Luo, Xingguang</au><au>Zheng, Xiong-Wei</au><au>Lin, Xiandong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>SNHG8 is identified as a key regulator of epstein-barr virus(EBV)-associated gastric cancer by an integrative analysis of lncRNA and mRNA expression</atitle><jtitle>Oncotarget</jtitle><addtitle>Oncotarget</addtitle><date>2016-12-06</date><risdate>2016</risdate><volume>7</volume><issue>49</issue><spage>80990</spage><epage>81002</epage><pages>80990-81002</pages><issn>1949-2553</issn><eissn>1949-2553</eissn><abstract>The Epstein-Barr virus (EBV) is associated with a variety of cancers, including gastric cancer, which has one of the highest mortality rates of all human cancers. Long non-coding RNAs (lncRNAs) have been suggested to have important causal roles in gastric cancer. However, the interaction between lncRNAs and EBV has not yet been studied. To this end, we sequenced 11,311 lncRNAs and 144,826 protein-coding transcripts from four types of tissue: one non-EBV-infected gastric carcinoma (EBVnGC) and its adjacent normal tissue, and one EBV-associated gastric carcinoma (EBVaGC) and its adjacent normal tissue. Five lncRNAs showed EBVaGC-specific expression; of those, one (SNHG8) was validated using real-time PCR in an independent cohort with 88 paired gastric cancer and adjacent tissue samples. To explore the functions of SNHG8, we identified its mRNA targets on the lncRNA-mRNA co-expression network of the Illumina Body Map, which contains the RNA sequencing data of mRNAs and lncRNAs from 16 normal human tissues. SNHG8 lncRNA was found to affect several gastric cancer-specific pathways and target genes of EBV. Our results reveal the intertwined tumorigenesis mechanisms of lncRNA and EBV and identify SNHG8 as a highly possible candidate biomarker and drug target of gastric cancer.</abstract><cop>United States</cop><pub>Impact Journals LLC</pub><pmid>27835598</pmid><doi>10.18632/oncotarget.13167</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Aged Cell Transformation, Viral Computational Biology Databases, Genetic Epstein-Barr Virus Infections - virology Female Gene Expression Profiling Gene Expression Regulation, Neoplastic Gene Regulatory Networks Herpesvirus 4, Human - pathogenicity Host-Pathogen Interactions Humans Male Middle Aged Real-Time Polymerase Chain Reaction Research Paper RNA, Long Noncoding - genetics RNA, Messenger - genetics RNA, Neoplasm - genetics Sequence Analysis, RNA Stomach Neoplasms - genetics Stomach Neoplasms - pathology Stomach Neoplasms - virology Young Adult |
| title | SNHG8 is identified as a key regulator of epstein-barr virus(EBV)-associated gastric cancer by an integrative analysis of lncRNA and mRNA expression |
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