Antigenic and genetic characterization of influenza viruses circulating in Bulgaria during the 2015/2016 season
Influenza virological surveillance is an essential tool for early detection of novel genetic variants of epidemiologic and clinical significance. The aim of this study was to determine the antigenic and molecular characteristics of influenza viruses circulating in Bulgaria during the 2015/2016 seaso...
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| Veröffentlicht in: | Infection, genetics and evolution genetics and evolution, 2017-04, Vol.49, p.241-250 |
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| creator | Korsun, Neli Angelova, Svetla Gregory, Viki Daniels, Rodney Georgieva, Irina McCauley, John |
| description | Influenza virological surveillance is an essential tool for early detection of novel genetic variants of epidemiologic and clinical significance. The aim of this study was to determine the antigenic and molecular characteristics of influenza viruses circulating in Bulgaria during the 2015/2016 season. The season was characterized by dominant circulation of A(H1N1)pdm09 viruses, accounting for 66% of detected influenza viruses, followed by B/Victoria-lineage viruses (24%) and A(H3N2) viruses (10%). All sequenced influenza A(H1N1)pdm09, A(H3N2) and B/Victoria-lineage viruses belonged to the 6B.1, 3C.2a and 1A genetic groups, respectively. Amino acid analysis of 57 A(H1N1)pdm09 isolates revealed the presence of 16 changes in hemagglutinin (HA) compared to the vaccine virus, five of which occurred in four antigenic sites, together with 16 changes in neuraminidase (NA) and a number of substitutions in proteins MP, NP, NS and PB2. Despite the many amino acid substitutions, A(H1N1)pdm09 viruses remained antigenically closely related to A/California/7/2009 vaccine virus. Bulgarian A(H3N2) strains (subclade 3C.2a) showed changes at 11 HA positions four of which were located in antigenic sites A and B, together with 6 positions in NA, compared to the subclade 3C.3a vaccine virus. They contained unique HA1 substitutions N171K, S312R and HA2 substitutions I77V and G155E compared to Bulgarian 3C.2a viruses of the previous season. All 20 B/Victoria-lineage viruses sequenced harboured two substitutions in the antigenic 120-loop region of HA, and 5 changes in NA, compared to the B/Brisbane/60/2008 vaccine virus. The results of this study reaffirm the continuous genetic variability of circulating seasonal influenza viruses and the need for continued systematic antigenic and molecular surveillance.
•6B.1, 3C.2a and 1A were the most prevalent genetic subclades of A(H1N1)pdm09, A(H3N2) and B/Victoria viruses, respectively.•Differences in antigenic sites, RBS and N-glycosylation motifs in HA and NA between epidemic and vaccine viruses were found.•Amino acid substitutions in internal proteins M, NP, NS1 and PB2 were identified.•All influenza A viruses were resistant to M2 blockers but both A/B viruses were susceptible to oseltamivir and zanamivir. |
| doi_str_mv | 10.1016/j.meegid.2017.01.027 |
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•6B.1, 3C.2a and 1A were the most prevalent genetic subclades of A(H1N1)pdm09, A(H3N2) and B/Victoria viruses, respectively.•Differences in antigenic sites, RBS and N-glycosylation motifs in HA and NA between epidemic and vaccine viruses were found.•Amino acid substitutions in internal proteins M, NP, NS1 and PB2 were identified.•All influenza A viruses were resistant to M2 blockers but both A/B viruses were susceptible to oseltamivir and zanamivir.</description><identifier>ISSN: 1567-1348</identifier><identifier>EISSN: 1567-7257</identifier><identifier>DOI: 10.1016/j.meegid.2017.01.027</identifier><identifier>PMID: 28132927</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Adolescent ; Adult ; Aged ; Amino Acid Sequence ; Amino Acid Substitution ; amino acids ; Antigenic and genetic characterization ; Antigens, Viral - genetics ; Bulgaria ; Bulgaria - epidemiology ; Child ; Child, Preschool ; Epidemiological Monitoring ; Female ; genetic variation ; Hemagglutinin Glycoproteins, Influenza Virus - genetics ; hemagglutinins ; Humans ; Infant ; Infant, Newborn ; influenza ; Influenza A virus ; Influenza A Virus, H1N1 Subtype - classification ; Influenza A Virus, H1N1 Subtype - genetics ; Influenza A Virus, H3N2 Subtype - classification ; Influenza A Virus, H3N2 Subtype - genetics ; Influenza virus ; Influenza, Human - epidemiology ; Influenza, Human - transmission ; Influenza, Human - virology ; Male ; Middle Aged ; monitoring ; Neuraminidase - genetics ; Phylogeny ; Research Paper ; Seasons ; Sequence Analysis, DNA ; sialidase ; vaccines ; viruses</subject><ispartof>Infection, genetics and evolution, 2017-04, Vol.49, p.241-250</ispartof><rights>2017 The Francis Crick Institute</rights><rights>Copyright © 2017 The Francis Crick Institute. Published by Elsevier B.V. All rights reserved.</rights><rights>2017 The Francis Crick Institute 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c562t-5363faec92e280e1bf9622b8f5b7816505d909705045959823c661d811337153</citedby><cites>FETCH-LOGICAL-c562t-5363faec92e280e1bf9622b8f5b7816505d909705045959823c661d811337153</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1567134817300278$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28132927$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Korsun, Neli</creatorcontrib><creatorcontrib>Angelova, Svetla</creatorcontrib><creatorcontrib>Gregory, Viki</creatorcontrib><creatorcontrib>Daniels, Rodney</creatorcontrib><creatorcontrib>Georgieva, Irina</creatorcontrib><creatorcontrib>McCauley, John</creatorcontrib><title>Antigenic and genetic characterization of influenza viruses circulating in Bulgaria during the 2015/2016 season</title><title>Infection, genetics and evolution</title><addtitle>Infect Genet Evol</addtitle><description>Influenza virological surveillance is an essential tool for early detection of novel genetic variants of epidemiologic and clinical significance. The aim of this study was to determine the antigenic and molecular characteristics of influenza viruses circulating in Bulgaria during the 2015/2016 season. The season was characterized by dominant circulation of A(H1N1)pdm09 viruses, accounting for 66% of detected influenza viruses, followed by B/Victoria-lineage viruses (24%) and A(H3N2) viruses (10%). All sequenced influenza A(H1N1)pdm09, A(H3N2) and B/Victoria-lineage viruses belonged to the 6B.1, 3C.2a and 1A genetic groups, respectively. Amino acid analysis of 57 A(H1N1)pdm09 isolates revealed the presence of 16 changes in hemagglutinin (HA) compared to the vaccine virus, five of which occurred in four antigenic sites, together with 16 changes in neuraminidase (NA) and a number of substitutions in proteins MP, NP, NS and PB2. Despite the many amino acid substitutions, A(H1N1)pdm09 viruses remained antigenically closely related to A/California/7/2009 vaccine virus. Bulgarian A(H3N2) strains (subclade 3C.2a) showed changes at 11 HA positions four of which were located in antigenic sites A and B, together with 6 positions in NA, compared to the subclade 3C.3a vaccine virus. They contained unique HA1 substitutions N171K, S312R and HA2 substitutions I77V and G155E compared to Bulgarian 3C.2a viruses of the previous season. All 20 B/Victoria-lineage viruses sequenced harboured two substitutions in the antigenic 120-loop region of HA, and 5 changes in NA, compared to the B/Brisbane/60/2008 vaccine virus. The results of this study reaffirm the continuous genetic variability of circulating seasonal influenza viruses and the need for continued systematic antigenic and molecular surveillance.
•6B.1, 3C.2a and 1A were the most prevalent genetic subclades of A(H1N1)pdm09, A(H3N2) and B/Victoria viruses, respectively.•Differences in antigenic sites, RBS and N-glycosylation motifs in HA and NA between epidemic and vaccine viruses were found.•Amino acid substitutions in internal proteins M, NP, NS1 and PB2 were identified.•All influenza A viruses were resistant to M2 blockers but both A/B viruses were susceptible to oseltamivir and zanamivir.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Amino Acid Sequence</subject><subject>Amino Acid Substitution</subject><subject>amino acids</subject><subject>Antigenic and genetic characterization</subject><subject>Antigens, Viral - genetics</subject><subject>Bulgaria</subject><subject>Bulgaria - epidemiology</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Epidemiological Monitoring</subject><subject>Female</subject><subject>genetic variation</subject><subject>Hemagglutinin Glycoproteins, Influenza Virus - genetics</subject><subject>hemagglutinins</subject><subject>Humans</subject><subject>Infant</subject><subject>Infant, Newborn</subject><subject>influenza</subject><subject>Influenza A virus</subject><subject>Influenza A Virus, H1N1 Subtype - classification</subject><subject>Influenza A Virus, H1N1 Subtype - genetics</subject><subject>Influenza A Virus, H3N2 Subtype - classification</subject><subject>Influenza A Virus, H3N2 Subtype - genetics</subject><subject>Influenza virus</subject><subject>Influenza, Human - epidemiology</subject><subject>Influenza, Human - transmission</subject><subject>Influenza, Human - virology</subject><subject>Male</subject><subject>Middle Aged</subject><subject>monitoring</subject><subject>Neuraminidase - genetics</subject><subject>Phylogeny</subject><subject>Research Paper</subject><subject>Seasons</subject><subject>Sequence Analysis, DNA</subject><subject>sialidase</subject><subject>vaccines</subject><subject>viruses</subject><issn>1567-1348</issn><issn>1567-7257</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UcFuEzEQtRCItoE_QMhHLtmO7djevSCVCihSJS69W453duNoYxfbG4l-fZ0mFLj0MvPkefNmPI-QDwwaBkxdbpsd4uj7hgPTDbAGuH5FzplUeqm51K9PmIlVe0Yuct5CJQJv35Iz3jLBO67PSbwKxY8YvKM29LQiLBW7jU3WFUz-wRYfA40D9WGYZgwPlu59mjNm6nxy81QJYaxV-mWeRpu8pf2cDk9lg7QuJy9rUDSjzTG8I28GO2V8f8oLcvft6931zfL25_cf11e3SycVL0splBgsuo4jbwHZeugU5-t2kGvdMiVB9h10GiSsZCe7lgunFOtbxoTQTIoF-XyUvZ_XO-wdhpLsZO6T39n020Trzf-V4DdmjHsj67VYVVmQTyeBFH_NmIvZ-exwmmzAOGfDAWAlNDxRV0eqSzHnhMPzGAbmYJXZmqNV5mCVAWaqVbXt478rPjf98ebvH7Deae8xmew8Boe9T-iK6aN_ecIjKr2mnA</recordid><startdate>201704</startdate><enddate>201704</enddate><creator>Korsun, Neli</creator><creator>Angelova, Svetla</creator><creator>Gregory, Viki</creator><creator>Daniels, Rodney</creator><creator>Georgieva, Irina</creator><creator>McCauley, John</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope></search><sort><creationdate>201704</creationdate><title>Antigenic and genetic characterization of influenza viruses circulating in Bulgaria during the 2015/2016 season</title><author>Korsun, Neli ; Angelova, Svetla ; Gregory, Viki ; Daniels, Rodney ; Georgieva, Irina ; McCauley, John</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c562t-5363faec92e280e1bf9622b8f5b7816505d909705045959823c661d811337153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Amino Acid Sequence</topic><topic>Amino Acid Substitution</topic><topic>amino acids</topic><topic>Antigenic and genetic characterization</topic><topic>Antigens, Viral - genetics</topic><topic>Bulgaria</topic><topic>Bulgaria - epidemiology</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Epidemiological Monitoring</topic><topic>Female</topic><topic>genetic variation</topic><topic>Hemagglutinin Glycoproteins, Influenza Virus - genetics</topic><topic>hemagglutinins</topic><topic>Humans</topic><topic>Infant</topic><topic>Infant, Newborn</topic><topic>influenza</topic><topic>Influenza A virus</topic><topic>Influenza A Virus, H1N1 Subtype - classification</topic><topic>Influenza A Virus, H1N1 Subtype - genetics</topic><topic>Influenza A Virus, H3N2 Subtype - classification</topic><topic>Influenza A Virus, H3N2 Subtype - genetics</topic><topic>Influenza virus</topic><topic>Influenza, Human - epidemiology</topic><topic>Influenza, Human - transmission</topic><topic>Influenza, Human - virology</topic><topic>Male</topic><topic>Middle Aged</topic><topic>monitoring</topic><topic>Neuraminidase - genetics</topic><topic>Phylogeny</topic><topic>Research Paper</topic><topic>Seasons</topic><topic>Sequence Analysis, DNA</topic><topic>sialidase</topic><topic>vaccines</topic><topic>viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Korsun, Neli</creatorcontrib><creatorcontrib>Angelova, Svetla</creatorcontrib><creatorcontrib>Gregory, Viki</creatorcontrib><creatorcontrib>Daniels, Rodney</creatorcontrib><creatorcontrib>Georgieva, Irina</creatorcontrib><creatorcontrib>McCauley, John</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Infection, genetics and evolution</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Korsun, Neli</au><au>Angelova, Svetla</au><au>Gregory, Viki</au><au>Daniels, Rodney</au><au>Georgieva, Irina</au><au>McCauley, John</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antigenic and genetic characterization of influenza viruses circulating in Bulgaria during the 2015/2016 season</atitle><jtitle>Infection, genetics and evolution</jtitle><addtitle>Infect Genet Evol</addtitle><date>2017-04</date><risdate>2017</risdate><volume>49</volume><spage>241</spage><epage>250</epage><pages>241-250</pages><issn>1567-1348</issn><eissn>1567-7257</eissn><abstract>Influenza virological surveillance is an essential tool for early detection of novel genetic variants of epidemiologic and clinical significance. The aim of this study was to determine the antigenic and molecular characteristics of influenza viruses circulating in Bulgaria during the 2015/2016 season. The season was characterized by dominant circulation of A(H1N1)pdm09 viruses, accounting for 66% of detected influenza viruses, followed by B/Victoria-lineage viruses (24%) and A(H3N2) viruses (10%). All sequenced influenza A(H1N1)pdm09, A(H3N2) and B/Victoria-lineage viruses belonged to the 6B.1, 3C.2a and 1A genetic groups, respectively. Amino acid analysis of 57 A(H1N1)pdm09 isolates revealed the presence of 16 changes in hemagglutinin (HA) compared to the vaccine virus, five of which occurred in four antigenic sites, together with 16 changes in neuraminidase (NA) and a number of substitutions in proteins MP, NP, NS and PB2. Despite the many amino acid substitutions, A(H1N1)pdm09 viruses remained antigenically closely related to A/California/7/2009 vaccine virus. Bulgarian A(H3N2) strains (subclade 3C.2a) showed changes at 11 HA positions four of which were located in antigenic sites A and B, together with 6 positions in NA, compared to the subclade 3C.3a vaccine virus. They contained unique HA1 substitutions N171K, S312R and HA2 substitutions I77V and G155E compared to Bulgarian 3C.2a viruses of the previous season. All 20 B/Victoria-lineage viruses sequenced harboured two substitutions in the antigenic 120-loop region of HA, and 5 changes in NA, compared to the B/Brisbane/60/2008 vaccine virus. The results of this study reaffirm the continuous genetic variability of circulating seasonal influenza viruses and the need for continued systematic antigenic and molecular surveillance.
•6B.1, 3C.2a and 1A were the most prevalent genetic subclades of A(H1N1)pdm09, A(H3N2) and B/Victoria viruses, respectively.•Differences in antigenic sites, RBS and N-glycosylation motifs in HA and NA between epidemic and vaccine viruses were found.•Amino acid substitutions in internal proteins M, NP, NS1 and PB2 were identified.•All influenza A viruses were resistant to M2 blockers but both A/B viruses were susceptible to oseltamivir and zanamivir.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>28132927</pmid><doi>10.1016/j.meegid.2017.01.027</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adolescent Adult Aged Amino Acid Sequence Amino Acid Substitution amino acids Antigenic and genetic characterization Antigens, Viral - genetics Bulgaria Bulgaria - epidemiology Child Child, Preschool Epidemiological Monitoring Female genetic variation Hemagglutinin Glycoproteins, Influenza Virus - genetics hemagglutinins Humans Infant Infant, Newborn influenza Influenza A virus Influenza A Virus, H1N1 Subtype - classification Influenza A Virus, H1N1 Subtype - genetics Influenza A Virus, H3N2 Subtype - classification Influenza A Virus, H3N2 Subtype - genetics Influenza virus Influenza, Human - epidemiology Influenza, Human - transmission Influenza, Human - virology Male Middle Aged monitoring Neuraminidase - genetics Phylogeny Research Paper Seasons Sequence Analysis, DNA sialidase vaccines viruses |
| title | Antigenic and genetic characterization of influenza viruses circulating in Bulgaria during the 2015/2016 season |
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