Protocol for qRT-PCR analysis from formalin fixed paraffin embedded tissue sections from diffuse large b-cell lymphoma: Validation of the six-gene predictor score

As a part of an international study on the molecular analysis of Diffuse Large B-cell Lymphoma (DLBCL), a robust protocol for gene expression analysis from RNA extraction to qRT-PCR using Formalin Fixed Paraffin Embedded tissues was developed. Here a study was conducted to define a strategy to valid...

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Veröffentlicht in:	Oncotarget 2016-12, Vol.7 (50), p.83319-83329
Hauptverfasser:	Tekin, Nilgun, Omidvar, Nader, Morris, Tim Peter, Conget, Paulette, Bruna, Flavia, Timar, Botond, Gagyi, Eva, Basak, Ranjan, Naik, Omkar, Auewarakul, Chirayu, Sritana, Narongrit, Levy, Debora, Cerci, Juliano Julio, Bydlowski, Sergio Paulo, Pereira, Juliana, Dimamay, Mark Pierre, Natividad, Filipinas, Chung, June-Key, Belder, Nevin, Kuzu, Isinsu, Paez, Diana, Dondi, Maurizio, Carr, Robert, Ozdag, Hilal, Padua, Rose Ann
Format:	Artikel
Sprache:	eng
Schlagworte:	Aged Asia Biomarkers, Tumor - genetics Biopsy Disease-Free Survival Europe Female Fixatives - chemistry Formaldehyde - chemistry Gene Expression Profiling - methods Gene Expression Profiling - standards Genetic Predisposition to Disease Humans Kaplan-Meier Estimate Lymphoma, Large B-Cell, Diffuse - drug therapy Lymphoma, Large B-Cell, Diffuse - genetics Lymphoma, Large B-Cell, Diffuse - mortality Lymphoma, Large B-Cell, Diffuse - pathology Male Middle Aged Paraffin Embedding Phenotype Predictive Value of Tests Proportional Hazards Models Real-Time Polymerase Chain Reaction - standards Reproducibility of Results Research Paper Reverse Transcriptase Polymerase Chain Reaction - standards South America Time Factors Tissue Fixation - methods Transcriptome
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