Nondestructive nanostraw intracellular sampling for longitudinal cell monitoring

Here, we report a method for time-resolved, longitudinal extraction and quantitative measurement of intracellular proteins and mRNA from a variety of cell types. Cytosolic contents were repeatedly sampled from the same cell or population of cells for more than 5 d through a cell-culture substrate, i...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 2017-03, Vol.114 (10), p.E1866-E1874
Hauptverfasser:	Cao, Yuhong, Hjort, Martin, Chen, Haodong, Birey, Fikri, Leal-Ortiz, Sergio A., Han, Crystal M., Santiago, Juan G., Paşca, Sergiu P., Wu, Joseph C., Melosh, Nicholas A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bioassays Biological Sciences Cardiomyocytes Cell culture Cellular biology Physical Sciences PNAS Plus Polymerase chain reaction RNA-protein interactions Stem cells
Online-Zugang:	Volltext
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