Inversion of Extender Unit Selectivity in the Erythromycin Polyketide Synthase by Acyltransferase Domain Engineering

Acyltransferase (AT) domains of polyketide synthases (PKSs) select extender units for incorporation into polyketides and dictate large portions of the structures of clinically relevant natural products. Accordingly, there is significant interest in engineering the substrate specificity of PKS ATs in...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	ACS chemical biology 2017-01, Vol.12 (1), p.114-123
Hauptverfasser:	Koryakina, Irina, Kasey, Christian, McArthur, John B, Lowell, Andrew N, Chemler, Joseph A, Li, Shasha, Hansen, Douglas A, Sherman, David H, Williams, Gavin J
Format:	Artikel
Sprache:	eng
Schlagworte:	Acyltransferases - chemistry Acyltransferases - genetics Acyltransferases - metabolism Erythromycin - chemistry Erythromycin - metabolism Macrolides - chemistry Macrolides - metabolism Mutagenesis, Site-Directed - methods Point Mutation Polyketide Synthases - chemistry Polyketide Synthases - genetics Polyketide Synthases - metabolism Polyketides - chemistry Polyketides - metabolism Protein Domains Saccharopolyspora - enzymology Saccharopolyspora - genetics Saccharopolyspora - metabolism Substrate Specificity
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!