Modulations of SIR-nucleosome interactions of reconstructed yeast silent pre-heterochromatin by O-acetyl-ADP-ribose and magnesium

Yeast silent heterochromatin provides an excellent model with which to study epigenetic inheritance. Previously we developed an in vitro assembly system to demonstrate the formation of filament structures with requirements that mirror yeast epigenetic gene silencing in vivo. However, the properties...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Molecular biology of the cell 2017-02, Vol.28 (3), p.381-386
Hauptverfasser: Tung, Shu-Yun, Wang, Sue-Hong, Lee, Sue-Ping, Tsai, Shu-Ping, Shen, Hsiao-Hsuian, Chen, Feng-Jung, Wu, Yu-Yi, Hsiao, Sheng-Pin, Liou, Gunn-Guang
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 386
container_issue 3
container_start_page 381
container_title Molecular biology of the cell
container_volume 28
creator Tung, Shu-Yun
Wang, Sue-Hong
Lee, Sue-Ping
Tsai, Shu-Ping
Shen, Hsiao-Hsuian
Chen, Feng-Jung
Wu, Yu-Yi
Hsiao, Sheng-Pin
Liou, Gunn-Guang
description Yeast silent heterochromatin provides an excellent model with which to study epigenetic inheritance. Previously we developed an in vitro assembly system to demonstrate the formation of filament structures with requirements that mirror yeast epigenetic gene silencing in vivo. However, the properties of these filaments were not investigated in detail. Here we show that the assembly system requires Sir2, Sir3, Sir4, nucleosomes, and O-acetyl-ADP-ribose. We also demonstrate that all Sir proteins and nucleosomes are components of these filaments to prove that they are SIR-nucleosome filaments. Furthermore, we show that the individual localization patterns of Sir proteins on the SIR-nucleosome filament reflect those patterns on telomeres in vivo. In addition, we reveal that magnesium exists in the SIR-nucleosome filament, with a role similar to that for chromatin condensation. These results suggest that a small number of proteins and molecules are sufficient to mediate the formation of a minimal yeast silent pre-heterochromatin in vitro.
doi_str_mv 10.1091/mbc.E16-06-0359
format Article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5341722</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1847889860</sourcerecordid><originalsourceid>FETCH-LOGICAL-c352t-c13806a21c339f7abfe5461103d9b5bb50143ba304b67267bedb1c43cc4ad3c13</originalsourceid><addsrcrecordid>eNpVUU1rFTEUDaLYD127kyzdpE0mHzPZCKW2tVCptLoOSeZOX2QmeSYZ4S395420PhQu3Avn3HPP5SD0jtETRjU7XZw_uWCK0FZc6hfokGmuiZCDetlmKjVhshMH6KiUH5QyIVT_Gh10vead0PIQ_f6SxnW2NaRYcJrw_fUdiaufIZW0AA6xQrZ-D2fwbap59RVGvANbKi5hhljxNgPZQKMnv8lpaZIRux2-JdZD3c3k7NNXkoNLBbCNI17sQ4QS1uUNejXZucDb536Mvl9efDv_TG5ur67Pz26I57KrxDM-UGU75jnXU2_dBFIoxigftZPOyfYcd5ZT4VTfqd7B6JgX3HthR962j9HHJ93t6hYYffOc7Wy2OSw270yywfyPxLAxD-mXkVywvuuawIdngZx-rlCqWULxMM82QlqLYYPoh0EPijbq6RPV51RKhml_hlHzJzjTgjPAlKGtWnBt4_2_7vb8v0nxR1GImKo</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1847889860</pqid></control><display><type>article</type><title>Modulations of SIR-nucleosome interactions of reconstructed yeast silent pre-heterochromatin by O-acetyl-ADP-ribose and magnesium</title><source>MEDLINE</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Tung, Shu-Yun ; Wang, Sue-Hong ; Lee, Sue-Ping ; Tsai, Shu-Ping ; Shen, Hsiao-Hsuian ; Chen, Feng-Jung ; Wu, Yu-Yi ; Hsiao, Sheng-Pin ; Liou, Gunn-Guang</creator><contributor>Weis, Karsten</contributor><creatorcontrib>Tung, Shu-Yun ; Wang, Sue-Hong ; Lee, Sue-Ping ; Tsai, Shu-Ping ; Shen, Hsiao-Hsuian ; Chen, Feng-Jung ; Wu, Yu-Yi ; Hsiao, Sheng-Pin ; Liou, Gunn-Guang ; Weis, Karsten</creatorcontrib><description>Yeast silent heterochromatin provides an excellent model with which to study epigenetic inheritance. Previously we developed an in vitro assembly system to demonstrate the formation of filament structures with requirements that mirror yeast epigenetic gene silencing in vivo. However, the properties of these filaments were not investigated in detail. Here we show that the assembly system requires Sir2, Sir3, Sir4, nucleosomes, and O-acetyl-ADP-ribose. We also demonstrate that all Sir proteins and nucleosomes are components of these filaments to prove that they are SIR-nucleosome filaments. Furthermore, we show that the individual localization patterns of Sir proteins on the SIR-nucleosome filament reflect those patterns on telomeres in vivo. In addition, we reveal that magnesium exists in the SIR-nucleosome filament, with a role similar to that for chromatin condensation. These results suggest that a small number of proteins and molecules are sufficient to mediate the formation of a minimal yeast silent pre-heterochromatin in vitro.</description><identifier>ISSN: 1059-1524</identifier><identifier>EISSN: 1939-4586</identifier><identifier>DOI: 10.1091/mbc.E16-06-0359</identifier><identifier>PMID: 27932495</identifier><language>eng</language><publisher>United States: The American Society for Cell Biology</publisher><subject>Binding Sites ; Brief Reports ; Chromatin - metabolism ; Chromatin Assembly and Disassembly ; Epigenomics - methods ; Gene Silencing - physiology ; Heterochromatin - metabolism ; Histones - metabolism ; Magnesium ; Nucleosomes - metabolism ; O-Acetyl-ADP-Ribose - metabolism ; Protein Processing, Post-Translational ; Saccharomyces cerevisiae - metabolism ; Saccharomyces cerevisiae Proteins - metabolism ; Silent Information Regulator Proteins, Saccharomyces cerevisiae - genetics ; Silent Information Regulator Proteins, Saccharomyces cerevisiae - metabolism ; Sirtuins - metabolism ; Telomere - metabolism</subject><ispartof>Molecular biology of the cell, 2017-02, Vol.28 (3), p.381-386</ispartof><rights>2017 Tung et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).</rights><rights>2017 Tung This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License ( ). 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c352t-c13806a21c339f7abfe5461103d9b5bb50143ba304b67267bedb1c43cc4ad3c13</citedby><cites>FETCH-LOGICAL-c352t-c13806a21c339f7abfe5461103d9b5bb50143ba304b67267bedb1c43cc4ad3c13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5341722/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5341722/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,729,782,786,887,27931,27932,53798,53800</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27932495$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Weis, Karsten</contributor><creatorcontrib>Tung, Shu-Yun</creatorcontrib><creatorcontrib>Wang, Sue-Hong</creatorcontrib><creatorcontrib>Lee, Sue-Ping</creatorcontrib><creatorcontrib>Tsai, Shu-Ping</creatorcontrib><creatorcontrib>Shen, Hsiao-Hsuian</creatorcontrib><creatorcontrib>Chen, Feng-Jung</creatorcontrib><creatorcontrib>Wu, Yu-Yi</creatorcontrib><creatorcontrib>Hsiao, Sheng-Pin</creatorcontrib><creatorcontrib>Liou, Gunn-Guang</creatorcontrib><title>Modulations of SIR-nucleosome interactions of reconstructed yeast silent pre-heterochromatin by O-acetyl-ADP-ribose and magnesium</title><title>Molecular biology of the cell</title><addtitle>Mol Biol Cell</addtitle><description>Yeast silent heterochromatin provides an excellent model with which to study epigenetic inheritance. Previously we developed an in vitro assembly system to demonstrate the formation of filament structures with requirements that mirror yeast epigenetic gene silencing in vivo. However, the properties of these filaments were not investigated in detail. Here we show that the assembly system requires Sir2, Sir3, Sir4, nucleosomes, and O-acetyl-ADP-ribose. We also demonstrate that all Sir proteins and nucleosomes are components of these filaments to prove that they are SIR-nucleosome filaments. Furthermore, we show that the individual localization patterns of Sir proteins on the SIR-nucleosome filament reflect those patterns on telomeres in vivo. In addition, we reveal that magnesium exists in the SIR-nucleosome filament, with a role similar to that for chromatin condensation. These results suggest that a small number of proteins and molecules are sufficient to mediate the formation of a minimal yeast silent pre-heterochromatin in vitro.</description><subject>Binding Sites</subject><subject>Brief Reports</subject><subject>Chromatin - metabolism</subject><subject>Chromatin Assembly and Disassembly</subject><subject>Epigenomics - methods</subject><subject>Gene Silencing - physiology</subject><subject>Heterochromatin - metabolism</subject><subject>Histones - metabolism</subject><subject>Magnesium</subject><subject>Nucleosomes - metabolism</subject><subject>O-Acetyl-ADP-Ribose - metabolism</subject><subject>Protein Processing, Post-Translational</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Saccharomyces cerevisiae Proteins - metabolism</subject><subject>Silent Information Regulator Proteins, Saccharomyces cerevisiae - genetics</subject><subject>Silent Information Regulator Proteins, Saccharomyces cerevisiae - metabolism</subject><subject>Sirtuins - metabolism</subject><subject>Telomere - metabolism</subject><issn>1059-1524</issn><issn>1939-4586</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVUU1rFTEUDaLYD127kyzdpE0mHzPZCKW2tVCptLoOSeZOX2QmeSYZ4S395420PhQu3Avn3HPP5SD0jtETRjU7XZw_uWCK0FZc6hfokGmuiZCDetlmKjVhshMH6KiUH5QyIVT_Gh10vead0PIQ_f6SxnW2NaRYcJrw_fUdiaufIZW0AA6xQrZ-D2fwbap59RVGvANbKi5hhljxNgPZQKMnv8lpaZIRux2-JdZD3c3k7NNXkoNLBbCNI17sQ4QS1uUNejXZucDb536Mvl9efDv_TG5ur67Pz26I57KrxDM-UGU75jnXU2_dBFIoxigftZPOyfYcd5ZT4VTfqd7B6JgX3HthR962j9HHJ93t6hYYffOc7Wy2OSw270yywfyPxLAxD-mXkVywvuuawIdngZx-rlCqWULxMM82QlqLYYPoh0EPijbq6RPV51RKhml_hlHzJzjTgjPAlKGtWnBt4_2_7vb8v0nxR1GImKo</recordid><startdate>20170201</startdate><enddate>20170201</enddate><creator>Tung, Shu-Yun</creator><creator>Wang, Sue-Hong</creator><creator>Lee, Sue-Ping</creator><creator>Tsai, Shu-Ping</creator><creator>Shen, Hsiao-Hsuian</creator><creator>Chen, Feng-Jung</creator><creator>Wu, Yu-Yi</creator><creator>Hsiao, Sheng-Pin</creator><creator>Liou, Gunn-Guang</creator><general>The American Society for Cell Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20170201</creationdate><title>Modulations of SIR-nucleosome interactions of reconstructed yeast silent pre-heterochromatin by O-acetyl-ADP-ribose and magnesium</title><author>Tung, Shu-Yun ; Wang, Sue-Hong ; Lee, Sue-Ping ; Tsai, Shu-Ping ; Shen, Hsiao-Hsuian ; Chen, Feng-Jung ; Wu, Yu-Yi ; Hsiao, Sheng-Pin ; Liou, Gunn-Guang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c352t-c13806a21c339f7abfe5461103d9b5bb50143ba304b67267bedb1c43cc4ad3c13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Binding Sites</topic><topic>Brief Reports</topic><topic>Chromatin - metabolism</topic><topic>Chromatin Assembly and Disassembly</topic><topic>Epigenomics - methods</topic><topic>Gene Silencing - physiology</topic><topic>Heterochromatin - metabolism</topic><topic>Histones - metabolism</topic><topic>Magnesium</topic><topic>Nucleosomes - metabolism</topic><topic>O-Acetyl-ADP-Ribose - metabolism</topic><topic>Protein Processing, Post-Translational</topic><topic>Saccharomyces cerevisiae - metabolism</topic><topic>Saccharomyces cerevisiae Proteins - metabolism</topic><topic>Silent Information Regulator Proteins, Saccharomyces cerevisiae - genetics</topic><topic>Silent Information Regulator Proteins, Saccharomyces cerevisiae - metabolism</topic><topic>Sirtuins - metabolism</topic><topic>Telomere - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tung, Shu-Yun</creatorcontrib><creatorcontrib>Wang, Sue-Hong</creatorcontrib><creatorcontrib>Lee, Sue-Ping</creatorcontrib><creatorcontrib>Tsai, Shu-Ping</creatorcontrib><creatorcontrib>Shen, Hsiao-Hsuian</creatorcontrib><creatorcontrib>Chen, Feng-Jung</creatorcontrib><creatorcontrib>Wu, Yu-Yi</creatorcontrib><creatorcontrib>Hsiao, Sheng-Pin</creatorcontrib><creatorcontrib>Liou, Gunn-Guang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Molecular biology of the cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tung, Shu-Yun</au><au>Wang, Sue-Hong</au><au>Lee, Sue-Ping</au><au>Tsai, Shu-Ping</au><au>Shen, Hsiao-Hsuian</au><au>Chen, Feng-Jung</au><au>Wu, Yu-Yi</au><au>Hsiao, Sheng-Pin</au><au>Liou, Gunn-Guang</au><au>Weis, Karsten</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Modulations of SIR-nucleosome interactions of reconstructed yeast silent pre-heterochromatin by O-acetyl-ADP-ribose and magnesium</atitle><jtitle>Molecular biology of the cell</jtitle><addtitle>Mol Biol Cell</addtitle><date>2017-02-01</date><risdate>2017</risdate><volume>28</volume><issue>3</issue><spage>381</spage><epage>386</epage><pages>381-386</pages><issn>1059-1524</issn><eissn>1939-4586</eissn><abstract>Yeast silent heterochromatin provides an excellent model with which to study epigenetic inheritance. Previously we developed an in vitro assembly system to demonstrate the formation of filament structures with requirements that mirror yeast epigenetic gene silencing in vivo. However, the properties of these filaments were not investigated in detail. Here we show that the assembly system requires Sir2, Sir3, Sir4, nucleosomes, and O-acetyl-ADP-ribose. We also demonstrate that all Sir proteins and nucleosomes are components of these filaments to prove that they are SIR-nucleosome filaments. Furthermore, we show that the individual localization patterns of Sir proteins on the SIR-nucleosome filament reflect those patterns on telomeres in vivo. In addition, we reveal that magnesium exists in the SIR-nucleosome filament, with a role similar to that for chromatin condensation. These results suggest that a small number of proteins and molecules are sufficient to mediate the formation of a minimal yeast silent pre-heterochromatin in vitro.</abstract><cop>United States</cop><pub>The American Society for Cell Biology</pub><pmid>27932495</pmid><doi>10.1091/mbc.E16-06-0359</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1059-1524
ispartof Molecular biology of the cell, 2017-02, Vol.28 (3), p.381-386
issn 1059-1524
1939-4586
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5341722
source MEDLINE; PubMed Central; Free Full-Text Journals in Chemistry
subjects Binding Sites
Brief Reports
Chromatin - metabolism
Chromatin Assembly and Disassembly
Epigenomics - methods
Gene Silencing - physiology
Heterochromatin - metabolism
Histones - metabolism
Magnesium
Nucleosomes - metabolism
O-Acetyl-ADP-Ribose - metabolism
Protein Processing, Post-Translational
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - metabolism
Silent Information Regulator Proteins, Saccharomyces cerevisiae - genetics
Silent Information Regulator Proteins, Saccharomyces cerevisiae - metabolism
Sirtuins - metabolism
Telomere - metabolism
title Modulations of SIR-nucleosome interactions of reconstructed yeast silent pre-heterochromatin by O-acetyl-ADP-ribose and magnesium
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-06T20%3A41%3A18IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Modulations%20of%20SIR-nucleosome%20interactions%20of%20reconstructed%20yeast%20silent%20pre-heterochromatin%20by%20O-acetyl-ADP-ribose%20and%20magnesium&rft.jtitle=Molecular%20biology%20of%20the%20cell&rft.au=Tung,%20Shu-Yun&rft.date=2017-02-01&rft.volume=28&rft.issue=3&rft.spage=381&rft.epage=386&rft.pages=381-386&rft.issn=1059-1524&rft.eissn=1939-4586&rft_id=info:doi/10.1091/mbc.E16-06-0359&rft_dat=%3Cproquest_pubme%3E1847889860%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1847889860&rft_id=info:pmid/27932495&rfr_iscdi=true