Modulations of SIR-nucleosome interactions of reconstructed yeast silent pre-heterochromatin by O-acetyl-ADP-ribose and magnesium

Yeast silent heterochromatin provides an excellent model with which to study epigenetic inheritance. Previously we developed an in vitro assembly system to demonstrate the formation of filament structures with requirements that mirror yeast epigenetic gene silencing in vivo. However, the properties...

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Veröffentlicht in:Molecular biology of the cell 2017-02, Vol.28 (3), p.381-386
Hauptverfasser: Tung, Shu-Yun, Wang, Sue-Hong, Lee, Sue-Ping, Tsai, Shu-Ping, Shen, Hsiao-Hsuian, Chen, Feng-Jung, Wu, Yu-Yi, Hsiao, Sheng-Pin, Liou, Gunn-Guang
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container_end_page 386
container_issue 3
container_start_page 381
container_title Molecular biology of the cell
container_volume 28
creator Tung, Shu-Yun
Wang, Sue-Hong
Lee, Sue-Ping
Tsai, Shu-Ping
Shen, Hsiao-Hsuian
Chen, Feng-Jung
Wu, Yu-Yi
Hsiao, Sheng-Pin
Liou, Gunn-Guang
description Yeast silent heterochromatin provides an excellent model with which to study epigenetic inheritance. Previously we developed an in vitro assembly system to demonstrate the formation of filament structures with requirements that mirror yeast epigenetic gene silencing in vivo. However, the properties of these filaments were not investigated in detail. Here we show that the assembly system requires Sir2, Sir3, Sir4, nucleosomes, and O-acetyl-ADP-ribose. We also demonstrate that all Sir proteins and nucleosomes are components of these filaments to prove that they are SIR-nucleosome filaments. Furthermore, we show that the individual localization patterns of Sir proteins on the SIR-nucleosome filament reflect those patterns on telomeres in vivo. In addition, we reveal that magnesium exists in the SIR-nucleosome filament, with a role similar to that for chromatin condensation. These results suggest that a small number of proteins and molecules are sufficient to mediate the formation of a minimal yeast silent pre-heterochromatin in vitro.
doi_str_mv 10.1091/mbc.E16-06-0359
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subjects Binding Sites
Brief Reports
Chromatin - metabolism
Chromatin Assembly and Disassembly
Epigenomics - methods
Gene Silencing - physiology
Heterochromatin - metabolism
Histones - metabolism
Magnesium
Nucleosomes - metabolism
O-Acetyl-ADP-Ribose - metabolism
Protein Processing, Post-Translational
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - metabolism
Silent Information Regulator Proteins, Saccharomyces cerevisiae - genetics
Silent Information Regulator Proteins, Saccharomyces cerevisiae - metabolism
Sirtuins - metabolism
Telomere - metabolism
title Modulations of SIR-nucleosome interactions of reconstructed yeast silent pre-heterochromatin by O-acetyl-ADP-ribose and magnesium
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