Modulations of SIR-nucleosome interactions of reconstructed yeast silent pre-heterochromatin by O-acetyl-ADP-ribose and magnesium
Yeast silent heterochromatin provides an excellent model with which to study epigenetic inheritance. Previously we developed an in vitro assembly system to demonstrate the formation of filament structures with requirements that mirror yeast epigenetic gene silencing in vivo. However, the properties...
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| Veröffentlicht in: | Molecular biology of the cell 2017-02, Vol.28 (3), p.381-386 |
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| creator | Tung, Shu-Yun Wang, Sue-Hong Lee, Sue-Ping Tsai, Shu-Ping Shen, Hsiao-Hsuian Chen, Feng-Jung Wu, Yu-Yi Hsiao, Sheng-Pin Liou, Gunn-Guang |
| description | Yeast silent heterochromatin provides an excellent model with which to study epigenetic inheritance. Previously we developed an in vitro assembly system to demonstrate the formation of filament structures with requirements that mirror yeast epigenetic gene silencing in vivo. However, the properties of these filaments were not investigated in detail. Here we show that the assembly system requires Sir2, Sir3, Sir4, nucleosomes, and O-acetyl-ADP-ribose. We also demonstrate that all Sir proteins and nucleosomes are components of these filaments to prove that they are SIR-nucleosome filaments. Furthermore, we show that the individual localization patterns of Sir proteins on the SIR-nucleosome filament reflect those patterns on telomeres in vivo. In addition, we reveal that magnesium exists in the SIR-nucleosome filament, with a role similar to that for chromatin condensation. These results suggest that a small number of proteins and molecules are sufficient to mediate the formation of a minimal yeast silent pre-heterochromatin in vitro. |
| doi_str_mv | 10.1091/mbc.E16-06-0359 |
| format | Article |
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Previously we developed an in vitro assembly system to demonstrate the formation of filament structures with requirements that mirror yeast epigenetic gene silencing in vivo. However, the properties of these filaments were not investigated in detail. Here we show that the assembly system requires Sir2, Sir3, Sir4, nucleosomes, and O-acetyl-ADP-ribose. We also demonstrate that all Sir proteins and nucleosomes are components of these filaments to prove that they are SIR-nucleosome filaments. Furthermore, we show that the individual localization patterns of Sir proteins on the SIR-nucleosome filament reflect those patterns on telomeres in vivo. In addition, we reveal that magnesium exists in the SIR-nucleosome filament, with a role similar to that for chromatin condensation. These results suggest that a small number of proteins and molecules are sufficient to mediate the formation of a minimal yeast silent pre-heterochromatin in vitro.</description><identifier>ISSN: 1059-1524</identifier><identifier>EISSN: 1939-4586</identifier><identifier>DOI: 10.1091/mbc.E16-06-0359</identifier><identifier>PMID: 27932495</identifier><language>eng</language><publisher>United States: The American Society for Cell Biology</publisher><subject>Binding Sites ; Brief Reports ; Chromatin - metabolism ; Chromatin Assembly and Disassembly ; Epigenomics - methods ; Gene Silencing - physiology ; Heterochromatin - metabolism ; Histones - metabolism ; Magnesium ; Nucleosomes - metabolism ; O-Acetyl-ADP-Ribose - metabolism ; Protein Processing, Post-Translational ; Saccharomyces cerevisiae - metabolism ; Saccharomyces cerevisiae Proteins - metabolism ; Silent Information Regulator Proteins, Saccharomyces cerevisiae - genetics ; Silent Information Regulator Proteins, Saccharomyces cerevisiae - metabolism ; Sirtuins - metabolism ; Telomere - metabolism</subject><ispartof>Molecular biology of the cell, 2017-02, Vol.28 (3), p.381-386</ispartof><rights>2017 Tung et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).</rights><rights>2017 Tung This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License ( ). 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c352t-c13806a21c339f7abfe5461103d9b5bb50143ba304b67267bedb1c43cc4ad3c13</citedby><cites>FETCH-LOGICAL-c352t-c13806a21c339f7abfe5461103d9b5bb50143ba304b67267bedb1c43cc4ad3c13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5341722/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5341722/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,729,782,786,887,27931,27932,53798,53800</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27932495$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Weis, Karsten</contributor><creatorcontrib>Tung, Shu-Yun</creatorcontrib><creatorcontrib>Wang, Sue-Hong</creatorcontrib><creatorcontrib>Lee, Sue-Ping</creatorcontrib><creatorcontrib>Tsai, Shu-Ping</creatorcontrib><creatorcontrib>Shen, Hsiao-Hsuian</creatorcontrib><creatorcontrib>Chen, Feng-Jung</creatorcontrib><creatorcontrib>Wu, Yu-Yi</creatorcontrib><creatorcontrib>Hsiao, Sheng-Pin</creatorcontrib><creatorcontrib>Liou, Gunn-Guang</creatorcontrib><title>Modulations of SIR-nucleosome interactions of reconstructed yeast silent pre-heterochromatin by O-acetyl-ADP-ribose and magnesium</title><title>Molecular biology of the cell</title><addtitle>Mol Biol Cell</addtitle><description>Yeast silent heterochromatin provides an excellent model with which to study epigenetic inheritance. Previously we developed an in vitro assembly system to demonstrate the formation of filament structures with requirements that mirror yeast epigenetic gene silencing in vivo. However, the properties of these filaments were not investigated in detail. Here we show that the assembly system requires Sir2, Sir3, Sir4, nucleosomes, and O-acetyl-ADP-ribose. We also demonstrate that all Sir proteins and nucleosomes are components of these filaments to prove that they are SIR-nucleosome filaments. Furthermore, we show that the individual localization patterns of Sir proteins on the SIR-nucleosome filament reflect those patterns on telomeres in vivo. In addition, we reveal that magnesium exists in the SIR-nucleosome filament, with a role similar to that for chromatin condensation. These results suggest that a small number of proteins and molecules are sufficient to mediate the formation of a minimal yeast silent pre-heterochromatin in vitro.</description><subject>Binding Sites</subject><subject>Brief Reports</subject><subject>Chromatin - metabolism</subject><subject>Chromatin Assembly and Disassembly</subject><subject>Epigenomics - methods</subject><subject>Gene Silencing - physiology</subject><subject>Heterochromatin - metabolism</subject><subject>Histones - metabolism</subject><subject>Magnesium</subject><subject>Nucleosomes - metabolism</subject><subject>O-Acetyl-ADP-Ribose - metabolism</subject><subject>Protein Processing, Post-Translational</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Saccharomyces cerevisiae Proteins - metabolism</subject><subject>Silent Information Regulator Proteins, Saccharomyces cerevisiae - genetics</subject><subject>Silent Information Regulator Proteins, Saccharomyces cerevisiae - metabolism</subject><subject>Sirtuins - metabolism</subject><subject>Telomere - metabolism</subject><issn>1059-1524</issn><issn>1939-4586</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVUU1rFTEUDaLYD127kyzdpE0mHzPZCKW2tVCptLoOSeZOX2QmeSYZ4S395420PhQu3Avn3HPP5SD0jtETRjU7XZw_uWCK0FZc6hfokGmuiZCDetlmKjVhshMH6KiUH5QyIVT_Gh10vead0PIQ_f6SxnW2NaRYcJrw_fUdiaufIZW0AA6xQrZ-D2fwbap59RVGvANbKi5hhljxNgPZQKMnv8lpaZIRux2-JdZD3c3k7NNXkoNLBbCNI17sQ4QS1uUNejXZucDb536Mvl9efDv_TG5ur67Pz26I57KrxDM-UGU75jnXU2_dBFIoxigftZPOyfYcd5ZT4VTfqd7B6JgX3HthR962j9HHJ93t6hYYffOc7Wy2OSw270yywfyPxLAxD-mXkVywvuuawIdngZx-rlCqWULxMM82QlqLYYPoh0EPijbq6RPV51RKhml_hlHzJzjTgjPAlKGtWnBt4_2_7vb8v0nxR1GImKo</recordid><startdate>20170201</startdate><enddate>20170201</enddate><creator>Tung, Shu-Yun</creator><creator>Wang, Sue-Hong</creator><creator>Lee, Sue-Ping</creator><creator>Tsai, Shu-Ping</creator><creator>Shen, Hsiao-Hsuian</creator><creator>Chen, Feng-Jung</creator><creator>Wu, Yu-Yi</creator><creator>Hsiao, Sheng-Pin</creator><creator>Liou, Gunn-Guang</creator><general>The American Society for Cell Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20170201</creationdate><title>Modulations of SIR-nucleosome interactions of reconstructed yeast silent pre-heterochromatin by O-acetyl-ADP-ribose and magnesium</title><author>Tung, Shu-Yun ; Wang, Sue-Hong ; Lee, Sue-Ping ; Tsai, Shu-Ping ; Shen, Hsiao-Hsuian ; Chen, Feng-Jung ; Wu, Yu-Yi ; Hsiao, Sheng-Pin ; Liou, Gunn-Guang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c352t-c13806a21c339f7abfe5461103d9b5bb50143ba304b67267bedb1c43cc4ad3c13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Binding Sites</topic><topic>Brief Reports</topic><topic>Chromatin - metabolism</topic><topic>Chromatin Assembly and Disassembly</topic><topic>Epigenomics - methods</topic><topic>Gene Silencing - physiology</topic><topic>Heterochromatin - metabolism</topic><topic>Histones - metabolism</topic><topic>Magnesium</topic><topic>Nucleosomes - metabolism</topic><topic>O-Acetyl-ADP-Ribose - metabolism</topic><topic>Protein Processing, Post-Translational</topic><topic>Saccharomyces cerevisiae - metabolism</topic><topic>Saccharomyces cerevisiae Proteins - metabolism</topic><topic>Silent Information Regulator Proteins, Saccharomyces cerevisiae - genetics</topic><topic>Silent Information Regulator Proteins, Saccharomyces cerevisiae - metabolism</topic><topic>Sirtuins - metabolism</topic><topic>Telomere - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tung, Shu-Yun</creatorcontrib><creatorcontrib>Wang, Sue-Hong</creatorcontrib><creatorcontrib>Lee, Sue-Ping</creatorcontrib><creatorcontrib>Tsai, Shu-Ping</creatorcontrib><creatorcontrib>Shen, Hsiao-Hsuian</creatorcontrib><creatorcontrib>Chen, Feng-Jung</creatorcontrib><creatorcontrib>Wu, Yu-Yi</creatorcontrib><creatorcontrib>Hsiao, Sheng-Pin</creatorcontrib><creatorcontrib>Liou, Gunn-Guang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Molecular biology of the cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tung, Shu-Yun</au><au>Wang, Sue-Hong</au><au>Lee, Sue-Ping</au><au>Tsai, Shu-Ping</au><au>Shen, Hsiao-Hsuian</au><au>Chen, Feng-Jung</au><au>Wu, Yu-Yi</au><au>Hsiao, Sheng-Pin</au><au>Liou, Gunn-Guang</au><au>Weis, Karsten</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Modulations of SIR-nucleosome interactions of reconstructed yeast silent pre-heterochromatin by O-acetyl-ADP-ribose and magnesium</atitle><jtitle>Molecular biology of the cell</jtitle><addtitle>Mol Biol Cell</addtitle><date>2017-02-01</date><risdate>2017</risdate><volume>28</volume><issue>3</issue><spage>381</spage><epage>386</epage><pages>381-386</pages><issn>1059-1524</issn><eissn>1939-4586</eissn><abstract>Yeast silent heterochromatin provides an excellent model with which to study epigenetic inheritance. Previously we developed an in vitro assembly system to demonstrate the formation of filament structures with requirements that mirror yeast epigenetic gene silencing in vivo. However, the properties of these filaments were not investigated in detail. Here we show that the assembly system requires Sir2, Sir3, Sir4, nucleosomes, and O-acetyl-ADP-ribose. We also demonstrate that all Sir proteins and nucleosomes are components of these filaments to prove that they are SIR-nucleosome filaments. Furthermore, we show that the individual localization patterns of Sir proteins on the SIR-nucleosome filament reflect those patterns on telomeres in vivo. In addition, we reveal that magnesium exists in the SIR-nucleosome filament, with a role similar to that for chromatin condensation. 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| subjects | Binding Sites Brief Reports Chromatin - metabolism Chromatin Assembly and Disassembly Epigenomics - methods Gene Silencing - physiology Heterochromatin - metabolism Histones - metabolism Magnesium Nucleosomes - metabolism O-Acetyl-ADP-Ribose - metabolism Protein Processing, Post-Translational Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins - metabolism Silent Information Regulator Proteins, Saccharomyces cerevisiae - genetics Silent Information Regulator Proteins, Saccharomyces cerevisiae - metabolism Sirtuins - metabolism Telomere - metabolism |
| title | Modulations of SIR-nucleosome interactions of reconstructed yeast silent pre-heterochromatin by O-acetyl-ADP-ribose and magnesium |
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