An Innovative Approach for Enhancing Bone Defect Healing Using PLGA Scaffolds Seeded with Extracorporeal-shock-wave-treated Bone Marrow Mesenchymal Stem Cells (BMSCs)
Although great efforts are being made using growth factors and gene therapy, the repair of bone defects remains a major challenge in modern medicine that has resulted in an increased burden on both healthcare and the economy. Emerging tissue engineering techniques that use of combination of biodegra...
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| Veröffentlicht in: | Scientific reports 2017-03, Vol.7 (1), p.44130-44130, Article 44130 |
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| creator | Chen, Youbin Xu, Jiankun Huang, Zhonglian Yu, Menglei Zhang, Yuantao Chen, Hongjiang Ma, Zebin Liao, Haojie Hu, Jun |
| description | Although great efforts are being made using growth factors and gene therapy, the repair of bone defects remains a major challenge in modern medicine that has resulted in an increased burden on both healthcare and the economy. Emerging tissue engineering techniques that use of combination of biodegradable poly-lactic-co-glycolic acid (PLGA) and mesenchymal stem cells have shed light on improving bone defect healing; however, additional growth factors are also required with these methods. Therefore, the development of novel and cost-effective approaches is of great importance. Our
in vitro
results demonstrated that ESW treatment (10 kV, 500 pulses) has a stimulatory effect on the proliferation and osteogenic differentiation of bone marrow-derived MSCs (BMSCs). Histological and micro-CT results showed that PLGA scaffolds seeded with ESW-treated BMSCs produced more bone-like tissue with commitment to the osteogenic lineage when subcutaneously implanted
in vivo
, as compared to control group. Significantly greater bone formation with a faster mineral apposition rate inside the defect site was observed in the ESW group compared to control group. Biomechanical parameters, including ultimate load and stress at failure, improved over time and were superior to those of the control group. Taken together, this innovative approach shows significant potential in bone tissue regeneration. |
| doi_str_mv | 10.1038/srep44130 |
| format | Article |
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in vitro
results demonstrated that ESW treatment (10 kV, 500 pulses) has a stimulatory effect on the proliferation and osteogenic differentiation of bone marrow-derived MSCs (BMSCs). Histological and micro-CT results showed that PLGA scaffolds seeded with ESW-treated BMSCs produced more bone-like tissue with commitment to the osteogenic lineage when subcutaneously implanted
in vivo
, as compared to control group. Significantly greater bone formation with a faster mineral apposition rate inside the defect site was observed in the ESW group compared to control group. Biomechanical parameters, including ultimate load and stress at failure, improved over time and were superior to those of the control group. Taken together, this innovative approach shows significant potential in bone tissue regeneration.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep44130</identifier><identifier>PMID: 28272494</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13/100 ; 13/107 ; 13/51 ; 59/5 ; 631/61/51/1844/2319 ; 631/80/641/83 ; 82/80 ; Apposition ; Biodegradability ; Bone growth ; Bone healing ; Bone marrow ; Computed tomography ; Defects ; Gene therapy ; Glycolic acid ; Growth factors ; Humanities and Social Sciences ; Mesenchymal stem cells ; Mesenchyme ; multidisciplinary ; Osteogenesis ; Polylactide-co-glycolide ; Regeneration ; Science ; Stem cells ; Tissue engineering</subject><ispartof>Scientific reports, 2017-03, Vol.7 (1), p.44130-44130, Article 44130</ispartof><rights>The Author(s) 2017</rights><rights>Copyright Nature Publishing Group Mar 2017</rights><rights>Copyright © 2017, The Author(s) 2017 The Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-e9e4a469b3996e361c3003843a87b8ba653ad5e83e036649c935219fffbd00b03</citedby><cites>FETCH-LOGICAL-c438t-e9e4a469b3996e361c3003843a87b8ba653ad5e83e036649c935219fffbd00b03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5341040/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5341040/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,41096,42165,51551,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28272494$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Youbin</creatorcontrib><creatorcontrib>Xu, Jiankun</creatorcontrib><creatorcontrib>Huang, Zhonglian</creatorcontrib><creatorcontrib>Yu, Menglei</creatorcontrib><creatorcontrib>Zhang, Yuantao</creatorcontrib><creatorcontrib>Chen, Hongjiang</creatorcontrib><creatorcontrib>Ma, Zebin</creatorcontrib><creatorcontrib>Liao, Haojie</creatorcontrib><creatorcontrib>Hu, Jun</creatorcontrib><title>An Innovative Approach for Enhancing Bone Defect Healing Using PLGA Scaffolds Seeded with Extracorporeal-shock-wave-treated Bone Marrow Mesenchymal Stem Cells (BMSCs)</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Although great efforts are being made using growth factors and gene therapy, the repair of bone defects remains a major challenge in modern medicine that has resulted in an increased burden on both healthcare and the economy. Emerging tissue engineering techniques that use of combination of biodegradable poly-lactic-co-glycolic acid (PLGA) and mesenchymal stem cells have shed light on improving bone defect healing; however, additional growth factors are also required with these methods. Therefore, the development of novel and cost-effective approaches is of great importance. Our
in vitro
results demonstrated that ESW treatment (10 kV, 500 pulses) has a stimulatory effect on the proliferation and osteogenic differentiation of bone marrow-derived MSCs (BMSCs). Histological and micro-CT results showed that PLGA scaffolds seeded with ESW-treated BMSCs produced more bone-like tissue with commitment to the osteogenic lineage when subcutaneously implanted
in vivo
, as compared to control group. Significantly greater bone formation with a faster mineral apposition rate inside the defect site was observed in the ESW group compared to control group. Biomechanical parameters, including ultimate load and stress at failure, improved over time and were superior to those of the control group. Taken together, this innovative approach shows significant potential in bone tissue regeneration.</description><subject>13/100</subject><subject>13/107</subject><subject>13/51</subject><subject>59/5</subject><subject>631/61/51/1844/2319</subject><subject>631/80/641/83</subject><subject>82/80</subject><subject>Apposition</subject><subject>Biodegradability</subject><subject>Bone growth</subject><subject>Bone healing</subject><subject>Bone marrow</subject><subject>Computed tomography</subject><subject>Defects</subject><subject>Gene therapy</subject><subject>Glycolic acid</subject><subject>Growth factors</subject><subject>Humanities and Social Sciences</subject><subject>Mesenchymal stem cells</subject><subject>Mesenchyme</subject><subject>multidisciplinary</subject><subject>Osteogenesis</subject><subject>Polylactide-co-glycolide</subject><subject>Regeneration</subject><subject>Science</subject><subject>Stem cells</subject><subject>Tissue engineering</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>BENPR</sourceid><recordid>eNplklFv0zAQxy0EYtPYA18AWeJlmxRmx44bvyB1XdkmtQKp7NlynEuTkdiZnbbsC_E5celWFfCDbd397u873yH0npJPlLD8MnjoOaeMvELHKeFZkrI0fX1wP0KnITyQuLJUcirfoqM0T0cpl_wY_RpbfGetW-uhWQMe97132tS4ch5Pba2taewSXzkL-BoqMAO-Bd1ubfdhu3-b3Yzxwuiqcm0Z8AKghBJvmqHG05-D18b53vkYkoTamR_JRq8hGaJhiNgf2bn23m3wHAJYUz91usWLATo8gbYN-OxqvpiE83foTaXbAKfP5wm6_zL9PrlNZl9v7ibjWWI4y4cEJHDNhSyYlAKYoIaR-Eec6XxU5IUWGdNlBjkDwoTg0kiWpVRWVVWUhBSEnaDPO91-VXRQGrCxhlb1vum0f1JON-pvj21qtXRrlTFOCd8KnD0LePe4gjCorgkmlqItuFVQNB9lnAghRUQ__oM-uJW3sTxFJWFMcJrTSJ3vKONdiL2u9slQorYDoPYDENkPh9nvyZd2R-BiB4ToskvwB0_-p_YbWDe61A</recordid><startdate>20170308</startdate><enddate>20170308</enddate><creator>Chen, Youbin</creator><creator>Xu, Jiankun</creator><creator>Huang, Zhonglian</creator><creator>Yu, Menglei</creator><creator>Zhang, Yuantao</creator><creator>Chen, Hongjiang</creator><creator>Ma, Zebin</creator><creator>Liao, Haojie</creator><creator>Hu, Jun</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20170308</creationdate><title>An Innovative Approach for Enhancing Bone Defect Healing Using PLGA Scaffolds Seeded with Extracorporeal-shock-wave-treated Bone Marrow Mesenchymal Stem Cells (BMSCs)</title><author>Chen, Youbin ; 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Emerging tissue engineering techniques that use of combination of biodegradable poly-lactic-co-glycolic acid (PLGA) and mesenchymal stem cells have shed light on improving bone defect healing; however, additional growth factors are also required with these methods. Therefore, the development of novel and cost-effective approaches is of great importance. Our
in vitro
results demonstrated that ESW treatment (10 kV, 500 pulses) has a stimulatory effect on the proliferation and osteogenic differentiation of bone marrow-derived MSCs (BMSCs). Histological and micro-CT results showed that PLGA scaffolds seeded with ESW-treated BMSCs produced more bone-like tissue with commitment to the osteogenic lineage when subcutaneously implanted
in vivo
, as compared to control group. Significantly greater bone formation with a faster mineral apposition rate inside the defect site was observed in the ESW group compared to control group. Biomechanical parameters, including ultimate load and stress at failure, improved over time and were superior to those of the control group. Taken together, this innovative approach shows significant potential in bone tissue regeneration.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>28272494</pmid><doi>10.1038/srep44130</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 13/100 13/107 13/51 59/5 631/61/51/1844/2319 631/80/641/83 82/80 Apposition Biodegradability Bone growth Bone healing Bone marrow Computed tomography Defects Gene therapy Glycolic acid Growth factors Humanities and Social Sciences Mesenchymal stem cells Mesenchyme multidisciplinary Osteogenesis Polylactide-co-glycolide Regeneration Science Stem cells Tissue engineering |
| title | An Innovative Approach for Enhancing Bone Defect Healing Using PLGA Scaffolds Seeded with Extracorporeal-shock-wave-treated Bone Marrow Mesenchymal Stem Cells (BMSCs) |
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