Lactobacillus rhamnosus GG supernatant enhance neonatal resistance to systemic Escherichia coli K1 infection by accelerating development of intestinal defense
The objective of this study was to determine whether Lactobacillus rhamnosus GG culture supernatant (LCS) has a preventive effect against gut-derived systemic neonatal Escherichia coli (E. coli ) K1 infection. The preventive effects were evaluated in human colonic carcinoma cell line Caco-2 and neon...
Gespeichert in:
| Veröffentlicht in: | Scientific reports 2017-03, Vol.7 (1), p.43305, Article 43305 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 1 |
| container_start_page | 43305 |
| container_title | Scientific reports |
| container_volume | 7 |
| creator | He, Xiaolong Zeng, Qing Puthiyakunnon, Santhosh Zeng, Zhijie Yang, Weijun Qiu, Jiawen Du, Lei Boddu, Swapna Wu, Tongwei Cai, Danxian Huang, Sheng-He Cao, Hong |
| description | The objective of this study was to determine whether
Lactobacillus rhamnosus
GG culture supernatant (LCS) has a preventive effect against gut-derived systemic neonatal
Escherichia coli (E. coli
) K1 infection. The preventive effects were evaluated in human colonic carcinoma cell line Caco-2 and neonatal rat models. Our
in vitro
results showed that LCS could block adhesion, invasion and translocation of
E. coli
K1 to Caco-2 monolayer via up-regulating mucin production and maintaining intestinal integrity.
In vivo
experiments revealed that pre-treatment with LCS significantly decrease susceptibility of neonatal rats to oral
E. coli
K1 infection as reflected by reduced bacterial intestinal colonization, translocation, dissemination and systemic infections. Further, we found that LCS treated neonatal rats have higher intestinal expressions of Ki67, MUC2, ZO-1, IgA, mucin and lower barrier permeability than those in untreated rats. These results indicated that LCS could enhance neonatal resistance to systemic
E. coli
K1 infection via promoting maturation of neonatal intestinal defense. In conclusions, our findings suggested that LCS has a prophylactic effect against systemic
E. coli
K1 infection in neonates. Future studies aimed at identifying the specific active ingredients in LCS will be helpful in developing effective pharmacological strategies for preventing neonatal
E. coli
K1 infection. |
| doi_str_mv | 10.1038/srep43305 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5338013</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1903363918</sourcerecordid><originalsourceid>FETCH-LOGICAL-c471t-435fff2c79c88ffdaa84747c92714754d14eba9455a88b72a1edeb9ddbdb2c6f3</originalsourceid><addsrcrecordid>eNplkd9qFDEUxoMottRe9AUk4JXC6uTP7GRuBCl1Ky54Y69DJjnZSckkY5Ip7Mv4rKbduqyYmxzO-fF9J_kQuiLNR9Iw8SknmDljTfsCndOGtyvKKH15Up-hy5zvm3pa2nPSv0ZnVNA1XQtxjn5vlS5xUNp5v2ScRjWFmGu12eC8zJCCKioUDGFUQQMOEB87HifILpenXok473OByWl8k_UIyenRKayjd_g7wS5Y0MXFgIc9VlqDh6SKCzts4AF8nCeoDtFWsECugypvwELI8Aa9sspnuHy-L9Dd15uf17er7Y_Nt-sv25XmHSkrzlprLdVdr4Ww1igleMc73dOO8K7lhnAYVM_bVgkxdFQRMDD0xgxmoHpt2QX6fNCdl2ECo-tCSXk5JzeptJdROfnvJLhR7uKDbBkTDWFV4N2zQIq_lvoKeR-X-nk-S9I3jK1ZT0Sl3h8onWKuwdmjA2nkY5rymGZl356udCT_ZleBDwcg11HYQTqx_E_tD4v9rxk</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1903363918</pqid></control><display><type>article</type><title>Lactobacillus rhamnosus GG supernatant enhance neonatal resistance to systemic Escherichia coli K1 infection by accelerating development of intestinal defense</title><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Springer Nature OA Free Journals</source><source>Nature Free</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>He, Xiaolong ; Zeng, Qing ; Puthiyakunnon, Santhosh ; Zeng, Zhijie ; Yang, Weijun ; Qiu, Jiawen ; Du, Lei ; Boddu, Swapna ; Wu, Tongwei ; Cai, Danxian ; Huang, Sheng-He ; Cao, Hong</creator><creatorcontrib>He, Xiaolong ; Zeng, Qing ; Puthiyakunnon, Santhosh ; Zeng, Zhijie ; Yang, Weijun ; Qiu, Jiawen ; Du, Lei ; Boddu, Swapna ; Wu, Tongwei ; Cai, Danxian ; Huang, Sheng-He ; Cao, Hong</creatorcontrib><description>The objective of this study was to determine whether
Lactobacillus rhamnosus
GG culture supernatant (LCS) has a preventive effect against gut-derived systemic neonatal
Escherichia coli (E. coli
) K1 infection. The preventive effects were evaluated in human colonic carcinoma cell line Caco-2 and neonatal rat models. Our
in vitro
results showed that LCS could block adhesion, invasion and translocation of
E. coli
K1 to Caco-2 monolayer via up-regulating mucin production and maintaining intestinal integrity.
In vivo
experiments revealed that pre-treatment with LCS significantly decrease susceptibility of neonatal rats to oral
E. coli
K1 infection as reflected by reduced bacterial intestinal colonization, translocation, dissemination and systemic infections. Further, we found that LCS treated neonatal rats have higher intestinal expressions of Ki67, MUC2, ZO-1, IgA, mucin and lower barrier permeability than those in untreated rats. These results indicated that LCS could enhance neonatal resistance to systemic
E. coli
K1 infection via promoting maturation of neonatal intestinal defense. In conclusions, our findings suggested that LCS has a prophylactic effect against systemic
E. coli
K1 infection in neonates. Future studies aimed at identifying the specific active ingredients in LCS will be helpful in developing effective pharmacological strategies for preventing neonatal
E. coli
K1 infection.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep43305</identifier><identifier>PMID: 28262688</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13/1 ; 13/106 ; 631/250/255/1318 ; 631/326/421 ; 692/420/254 ; Animal models ; Cell culture ; Colonization ; E coli ; Escherichia coli ; Gastrointestinal tract ; Humanities and Social Sciences ; Immunoglobulin A ; Infections ; Intestine ; Mucin ; multidisciplinary ; Neonates ; Permeability ; Rodents ; Science ; Translocation ; Zonula occludens-1 protein</subject><ispartof>Scientific reports, 2017-03, Vol.7 (1), p.43305, Article 43305</ispartof><rights>The Author(s) 2017</rights><rights>Copyright Nature Publishing Group Mar 2017</rights><rights>Copyright © 2017, The Author(s) 2017 The Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c471t-435fff2c79c88ffdaa84747c92714754d14eba9455a88b72a1edeb9ddbdb2c6f3</citedby><cites>FETCH-LOGICAL-c471t-435fff2c79c88ffdaa84747c92714754d14eba9455a88b72a1edeb9ddbdb2c6f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5338013/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5338013/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27923,27924,41119,42188,51575,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28262688$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>He, Xiaolong</creatorcontrib><creatorcontrib>Zeng, Qing</creatorcontrib><creatorcontrib>Puthiyakunnon, Santhosh</creatorcontrib><creatorcontrib>Zeng, Zhijie</creatorcontrib><creatorcontrib>Yang, Weijun</creatorcontrib><creatorcontrib>Qiu, Jiawen</creatorcontrib><creatorcontrib>Du, Lei</creatorcontrib><creatorcontrib>Boddu, Swapna</creatorcontrib><creatorcontrib>Wu, Tongwei</creatorcontrib><creatorcontrib>Cai, Danxian</creatorcontrib><creatorcontrib>Huang, Sheng-He</creatorcontrib><creatorcontrib>Cao, Hong</creatorcontrib><title>Lactobacillus rhamnosus GG supernatant enhance neonatal resistance to systemic Escherichia coli K1 infection by accelerating development of intestinal defense</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>The objective of this study was to determine whether
Lactobacillus rhamnosus
GG culture supernatant (LCS) has a preventive effect against gut-derived systemic neonatal
Escherichia coli (E. coli
) K1 infection. The preventive effects were evaluated in human colonic carcinoma cell line Caco-2 and neonatal rat models. Our
in vitro
results showed that LCS could block adhesion, invasion and translocation of
E. coli
K1 to Caco-2 monolayer via up-regulating mucin production and maintaining intestinal integrity.
In vivo
experiments revealed that pre-treatment with LCS significantly decrease susceptibility of neonatal rats to oral
E. coli
K1 infection as reflected by reduced bacterial intestinal colonization, translocation, dissemination and systemic infections. Further, we found that LCS treated neonatal rats have higher intestinal expressions of Ki67, MUC2, ZO-1, IgA, mucin and lower barrier permeability than those in untreated rats. These results indicated that LCS could enhance neonatal resistance to systemic
E. coli
K1 infection via promoting maturation of neonatal intestinal defense. In conclusions, our findings suggested that LCS has a prophylactic effect against systemic
E. coli
K1 infection in neonates. Future studies aimed at identifying the specific active ingredients in LCS will be helpful in developing effective pharmacological strategies for preventing neonatal
E. coli
K1 infection.</description><subject>13/1</subject><subject>13/106</subject><subject>631/250/255/1318</subject><subject>631/326/421</subject><subject>692/420/254</subject><subject>Animal models</subject><subject>Cell culture</subject><subject>Colonization</subject><subject>E coli</subject><subject>Escherichia coli</subject><subject>Gastrointestinal tract</subject><subject>Humanities and Social Sciences</subject><subject>Immunoglobulin A</subject><subject>Infections</subject><subject>Intestine</subject><subject>Mucin</subject><subject>multidisciplinary</subject><subject>Neonates</subject><subject>Permeability</subject><subject>Rodents</subject><subject>Science</subject><subject>Translocation</subject><subject>Zonula occludens-1 protein</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNplkd9qFDEUxoMottRe9AUk4JXC6uTP7GRuBCl1Ky54Y69DJjnZSckkY5Ip7Mv4rKbduqyYmxzO-fF9J_kQuiLNR9Iw8SknmDljTfsCndOGtyvKKH15Up-hy5zvm3pa2nPSv0ZnVNA1XQtxjn5vlS5xUNp5v2ScRjWFmGu12eC8zJCCKioUDGFUQQMOEB87HifILpenXok473OByWl8k_UIyenRKayjd_g7wS5Y0MXFgIc9VlqDh6SKCzts4AF8nCeoDtFWsECugypvwELI8Aa9sspnuHy-L9Dd15uf17er7Y_Nt-sv25XmHSkrzlprLdVdr4Ww1igleMc73dOO8K7lhnAYVM_bVgkxdFQRMDD0xgxmoHpt2QX6fNCdl2ECo-tCSXk5JzeptJdROfnvJLhR7uKDbBkTDWFV4N2zQIq_lvoKeR-X-nk-S9I3jK1ZT0Sl3h8onWKuwdmjA2nkY5rymGZl356udCT_ZleBDwcg11HYQTqx_E_tD4v9rxk</recordid><startdate>20170306</startdate><enddate>20170306</enddate><creator>He, Xiaolong</creator><creator>Zeng, Qing</creator><creator>Puthiyakunnon, Santhosh</creator><creator>Zeng, Zhijie</creator><creator>Yang, Weijun</creator><creator>Qiu, Jiawen</creator><creator>Du, Lei</creator><creator>Boddu, Swapna</creator><creator>Wu, Tongwei</creator><creator>Cai, Danxian</creator><creator>Huang, Sheng-He</creator><creator>Cao, Hong</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>5PM</scope></search><sort><creationdate>20170306</creationdate><title>Lactobacillus rhamnosus GG supernatant enhance neonatal resistance to systemic Escherichia coli K1 infection by accelerating development of intestinal defense</title><author>He, Xiaolong ; Zeng, Qing ; Puthiyakunnon, Santhosh ; Zeng, Zhijie ; Yang, Weijun ; Qiu, Jiawen ; Du, Lei ; Boddu, Swapna ; Wu, Tongwei ; Cai, Danxian ; Huang, Sheng-He ; Cao, Hong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c471t-435fff2c79c88ffdaa84747c92714754d14eba9455a88b72a1edeb9ddbdb2c6f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>13/1</topic><topic>13/106</topic><topic>631/250/255/1318</topic><topic>631/326/421</topic><topic>692/420/254</topic><topic>Animal models</topic><topic>Cell culture</topic><topic>Colonization</topic><topic>E coli</topic><topic>Escherichia coli</topic><topic>Gastrointestinal tract</topic><topic>Humanities and Social Sciences</topic><topic>Immunoglobulin A</topic><topic>Infections</topic><topic>Intestine</topic><topic>Mucin</topic><topic>multidisciplinary</topic><topic>Neonates</topic><topic>Permeability</topic><topic>Rodents</topic><topic>Science</topic><topic>Translocation</topic><topic>Zonula occludens-1 protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>He, Xiaolong</creatorcontrib><creatorcontrib>Zeng, Qing</creatorcontrib><creatorcontrib>Puthiyakunnon, Santhosh</creatorcontrib><creatorcontrib>Zeng, Zhijie</creatorcontrib><creatorcontrib>Yang, Weijun</creatorcontrib><creatorcontrib>Qiu, Jiawen</creatorcontrib><creatorcontrib>Du, Lei</creatorcontrib><creatorcontrib>Boddu, Swapna</creatorcontrib><creatorcontrib>Wu, Tongwei</creatorcontrib><creatorcontrib>Cai, Danxian</creatorcontrib><creatorcontrib>Huang, Sheng-He</creatorcontrib><creatorcontrib>Cao, Hong</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>He, Xiaolong</au><au>Zeng, Qing</au><au>Puthiyakunnon, Santhosh</au><au>Zeng, Zhijie</au><au>Yang, Weijun</au><au>Qiu, Jiawen</au><au>Du, Lei</au><au>Boddu, Swapna</au><au>Wu, Tongwei</au><au>Cai, Danxian</au><au>Huang, Sheng-He</au><au>Cao, Hong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lactobacillus rhamnosus GG supernatant enhance neonatal resistance to systemic Escherichia coli K1 infection by accelerating development of intestinal defense</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2017-03-06</date><risdate>2017</risdate><volume>7</volume><issue>1</issue><spage>43305</spage><pages>43305-</pages><artnum>43305</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>The objective of this study was to determine whether
Lactobacillus rhamnosus
GG culture supernatant (LCS) has a preventive effect against gut-derived systemic neonatal
Escherichia coli (E. coli
) K1 infection. The preventive effects were evaluated in human colonic carcinoma cell line Caco-2 and neonatal rat models. Our
in vitro
results showed that LCS could block adhesion, invasion and translocation of
E. coli
K1 to Caco-2 monolayer via up-regulating mucin production and maintaining intestinal integrity.
In vivo
experiments revealed that pre-treatment with LCS significantly decrease susceptibility of neonatal rats to oral
E. coli
K1 infection as reflected by reduced bacterial intestinal colonization, translocation, dissemination and systemic infections. Further, we found that LCS treated neonatal rats have higher intestinal expressions of Ki67, MUC2, ZO-1, IgA, mucin and lower barrier permeability than those in untreated rats. These results indicated that LCS could enhance neonatal resistance to systemic
E. coli
K1 infection via promoting maturation of neonatal intestinal defense. In conclusions, our findings suggested that LCS has a prophylactic effect against systemic
E. coli
K1 infection in neonates. Future studies aimed at identifying the specific active ingredients in LCS will be helpful in developing effective pharmacological strategies for preventing neonatal
E. coli
K1 infection.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>28262688</pmid><doi>10.1038/srep43305</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2045-2322 |
| ispartof | Scientific reports, 2017-03, Vol.7 (1), p.43305, Article 43305 |
| issn | 2045-2322 2045-2322 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5338013 |
| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Springer Nature OA Free Journals; Nature Free; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | 13/1 13/106 631/250/255/1318 631/326/421 692/420/254 Animal models Cell culture Colonization E coli Escherichia coli Gastrointestinal tract Humanities and Social Sciences Immunoglobulin A Infections Intestine Mucin multidisciplinary Neonates Permeability Rodents Science Translocation Zonula occludens-1 protein |
| title | Lactobacillus rhamnosus GG supernatant enhance neonatal resistance to systemic Escherichia coli K1 infection by accelerating development of intestinal defense |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-11T20%3A14%3A04IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Lactobacillus%20rhamnosus%20GG%20supernatant%20enhance%20neonatal%20resistance%20to%20systemic%20Escherichia%20coli%20K1%20infection%20by%20accelerating%20development%20of%20intestinal%20defense&rft.jtitle=Scientific%20reports&rft.au=He,%20Xiaolong&rft.date=2017-03-06&rft.volume=7&rft.issue=1&rft.spage=43305&rft.pages=43305-&rft.artnum=43305&rft.issn=2045-2322&rft.eissn=2045-2322&rft_id=info:doi/10.1038/srep43305&rft_dat=%3Cproquest_pubme%3E1903363918%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1903363918&rft_id=info:pmid/28262688&rfr_iscdi=true |