Characterization of the Porphyromonas gingivalis Type IX Secretion Trans-envelope PorKLMNP Core Complex
The transport of proteins at the cell surface of Bacteroidetes depends on a secretory apparatus known as type IX secretion system (T9SS). This machine is responsible for the cell surface exposition of various proteins, such as adhesins, required for gliding motility in , S-layer components in , and...
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| Veröffentlicht in: | The Journal of biological chemistry 2017-02, Vol.292 (8), p.3252-3261 |
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| creator | Vincent, Maxence S Canestrari, Mickaël J Leone, Philippe Stathopulos, Julien Ize, Bérengère Zoued, Abdelrahim Cambillau, Christian Kellenberger, Christine Roussel, Alain Cascales, Eric |
| description | The transport of proteins at the cell surface of Bacteroidetes depends on a secretory apparatus known as type IX secretion system (T9SS). This machine is responsible for the cell surface exposition of various proteins, such as adhesins, required for gliding motility in
, S-layer components in
, and tooth tissue-degrading enzymes in the oral pathogen
Although a number of subunits of the T9SS have been identified, we lack details on the architecture of this secretion apparatus. Here we provide evidence that five of the genes encoding the core complex of the T9SS are co-transcribed and that the gene products are distributed in the cell envelope. Protein-protein interaction studies then revealed that these proteins oligomerize and interact through a dense network of contacts. |
| doi_str_mv | 10.1074/jbc.m116.765081 |
| format | Article |
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, S-layer components in
, and tooth tissue-degrading enzymes in the oral pathogen
Although a number of subunits of the T9SS have been identified, we lack details on the architecture of this secretion apparatus. Here we provide evidence that five of the genes encoding the core complex of the T9SS are co-transcribed and that the gene products are distributed in the cell envelope. Protein-protein interaction studies then revealed that these proteins oligomerize and interact through a dense network of contacts.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1074/jbc.m116.765081</identifier><identifier>PMID: 28057754</identifier><language>eng</language><publisher>United States: American Society for Biochemistry and Molecular Biology</publisher><subject>Bacterial Proteins - analysis ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Bacterial Secretion Systems - analysis ; Bacterial Secretion Systems - genetics ; Bacterial Secretion Systems - metabolism ; Bacteriology ; Bacteroidaceae Infections - microbiology ; Biochemistry ; Biochemistry, Molecular Biology ; Biophysics ; Crystallography, X-Ray ; Genes, Bacterial ; Humans ; Life Sciences ; Microbiology ; Microbiology and Parasitology ; Molecular biology ; Molecular Networks ; Porphyromonas gingivalis - chemistry ; Porphyromonas gingivalis - genetics ; Porphyromonas gingivalis - metabolism ; Protein Interaction Maps ; Protein Subunits - analysis ; Protein Subunits - genetics ; Protein Subunits - metabolism ; Structural Biology</subject><ispartof>The Journal of biological chemistry, 2017-02, Vol.292 (8), p.3252-3261</ispartof><rights>2017 by The American Society for Biochemistry and Molecular Biology, Inc.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><rights>2017 by The American Society for Biochemistry and Molecular Biology, Inc. 2017 The American Society for Biochemistry and Molecular Biology, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c539t-f0b12f4aec8059be6f0f7b55bda113c716e39a6de2870c6e43ffe10b569cada93</citedby><cites>FETCH-LOGICAL-c539t-f0b12f4aec8059be6f0f7b55bda113c716e39a6de2870c6e43ffe10b569cada93</cites><orcidid>0000-0003-0611-9179 ; 0000-0001-9106-9254 ; 0000-0003-0425-5775 ; 0000-0001-5502-4729 ; 0000-0003-1902-3889</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5336160/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5336160/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,728,781,785,886,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28057754$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://amu.hal.science/hal-01780705$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Vincent, Maxence S</creatorcontrib><creatorcontrib>Canestrari, Mickaël J</creatorcontrib><creatorcontrib>Leone, Philippe</creatorcontrib><creatorcontrib>Stathopulos, Julien</creatorcontrib><creatorcontrib>Ize, Bérengère</creatorcontrib><creatorcontrib>Zoued, Abdelrahim</creatorcontrib><creatorcontrib>Cambillau, Christian</creatorcontrib><creatorcontrib>Kellenberger, Christine</creatorcontrib><creatorcontrib>Roussel, Alain</creatorcontrib><creatorcontrib>Cascales, Eric</creatorcontrib><title>Characterization of the Porphyromonas gingivalis Type IX Secretion Trans-envelope PorKLMNP Core Complex</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>The transport of proteins at the cell surface of Bacteroidetes depends on a secretory apparatus known as type IX secretion system (T9SS). This machine is responsible for the cell surface exposition of various proteins, such as adhesins, required for gliding motility in
, S-layer components in
, and tooth tissue-degrading enzymes in the oral pathogen
Although a number of subunits of the T9SS have been identified, we lack details on the architecture of this secretion apparatus. Here we provide evidence that five of the genes encoding the core complex of the T9SS are co-transcribed and that the gene products are distributed in the cell envelope. Protein-protein interaction studies then revealed that these proteins oligomerize and interact through a dense network of contacts.</description><subject>Bacterial Proteins - analysis</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bacterial Secretion Systems - analysis</subject><subject>Bacterial Secretion Systems - genetics</subject><subject>Bacterial Secretion Systems - metabolism</subject><subject>Bacteriology</subject><subject>Bacteroidaceae Infections - microbiology</subject><subject>Biochemistry</subject><subject>Biochemistry, Molecular Biology</subject><subject>Biophysics</subject><subject>Crystallography, X-Ray</subject><subject>Genes, Bacterial</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>Microbiology</subject><subject>Microbiology and Parasitology</subject><subject>Molecular biology</subject><subject>Molecular Networks</subject><subject>Porphyromonas gingivalis - chemistry</subject><subject>Porphyromonas gingivalis - genetics</subject><subject>Porphyromonas gingivalis - metabolism</subject><subject>Protein Interaction Maps</subject><subject>Protein Subunits - analysis</subject><subject>Protein Subunits - genetics</subject><subject>Protein Subunits - metabolism</subject><subject>Structural Biology</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkcFv0zAUxi0EYt3gzA3lCId0fnFsxxekqQI20cEkirSb5bjPjackDnZa0f31pOuYgHfwk56_73u2foS8AToHKsvzu9rOOwAxl4LTCp6RGdCK5YzD7XMyo7SAXBW8OiGnKd3RqUoFL8lJUVEuJS9nZLNoTDR2xOjvzehDnwWXjQ1mNyEOzT6GLvQmZRvfb_zOtD5lq_2A2dVt9h1txAfHKpo-5djvsA3Dg_PL8vrrTbYIEaejG1r89Yq8cKZN-Pqxn5Efnz6uFpf58tvnq8XFMrecqTF3tIbClQbt9EJVo3DUyZrzem0AmJUgkCkj1lhUklqBJXMOgdZcKGvWRrEz8uGYO2zrDtcW-zGaVg_RdybudTBe_3vT-0Zvwk5zxgQIOgW8PwY0_9kuL5b6MKMgKyop38Gkffe4LIafW0yj7nyy2Lamx7BNGiouuKKqOEjPj1IbQ0oR3VM2UH1AqSeU-npCqY8oJ8fbv3_ypP_Djv0GSGWcnQ</recordid><startdate>20170224</startdate><enddate>20170224</enddate><creator>Vincent, Maxence S</creator><creator>Canestrari, Mickaël J</creator><creator>Leone, Philippe</creator><creator>Stathopulos, Julien</creator><creator>Ize, Bérengère</creator><creator>Zoued, Abdelrahim</creator><creator>Cambillau, Christian</creator><creator>Kellenberger, Christine</creator><creator>Roussel, Alain</creator><creator>Cascales, Eric</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-0611-9179</orcidid><orcidid>https://orcid.org/0000-0001-9106-9254</orcidid><orcidid>https://orcid.org/0000-0003-0425-5775</orcidid><orcidid>https://orcid.org/0000-0001-5502-4729</orcidid><orcidid>https://orcid.org/0000-0003-1902-3889</orcidid></search><sort><creationdate>20170224</creationdate><title>Characterization of the Porphyromonas gingivalis Type IX Secretion Trans-envelope PorKLMNP Core Complex</title><author>Vincent, Maxence S ; Canestrari, Mickaël J ; Leone, Philippe ; Stathopulos, Julien ; Ize, Bérengère ; Zoued, Abdelrahim ; Cambillau, Christian ; Kellenberger, Christine ; Roussel, Alain ; Cascales, Eric</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c539t-f0b12f4aec8059be6f0f7b55bda113c716e39a6de2870c6e43ffe10b569cada93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Bacterial Proteins - analysis</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Bacterial Secretion Systems - analysis</topic><topic>Bacterial Secretion Systems - genetics</topic><topic>Bacterial Secretion Systems - metabolism</topic><topic>Bacteriology</topic><topic>Bacteroidaceae Infections - microbiology</topic><topic>Biochemistry</topic><topic>Biochemistry, Molecular Biology</topic><topic>Biophysics</topic><topic>Crystallography, X-Ray</topic><topic>Genes, Bacterial</topic><topic>Humans</topic><topic>Life Sciences</topic><topic>Microbiology</topic><topic>Microbiology and Parasitology</topic><topic>Molecular biology</topic><topic>Molecular Networks</topic><topic>Porphyromonas gingivalis - chemistry</topic><topic>Porphyromonas gingivalis - genetics</topic><topic>Porphyromonas gingivalis - metabolism</topic><topic>Protein Interaction Maps</topic><topic>Protein Subunits - analysis</topic><topic>Protein Subunits - genetics</topic><topic>Protein Subunits - metabolism</topic><topic>Structural Biology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vincent, Maxence S</creatorcontrib><creatorcontrib>Canestrari, Mickaël J</creatorcontrib><creatorcontrib>Leone, Philippe</creatorcontrib><creatorcontrib>Stathopulos, Julien</creatorcontrib><creatorcontrib>Ize, Bérengère</creatorcontrib><creatorcontrib>Zoued, Abdelrahim</creatorcontrib><creatorcontrib>Cambillau, Christian</creatorcontrib><creatorcontrib>Kellenberger, Christine</creatorcontrib><creatorcontrib>Roussel, Alain</creatorcontrib><creatorcontrib>Cascales, Eric</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vincent, Maxence S</au><au>Canestrari, Mickaël J</au><au>Leone, Philippe</au><au>Stathopulos, Julien</au><au>Ize, Bérengère</au><au>Zoued, Abdelrahim</au><au>Cambillau, Christian</au><au>Kellenberger, Christine</au><au>Roussel, Alain</au><au>Cascales, Eric</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of the Porphyromonas gingivalis Type IX Secretion Trans-envelope PorKLMNP Core Complex</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>2017-02-24</date><risdate>2017</risdate><volume>292</volume><issue>8</issue><spage>3252</spage><epage>3261</epage><pages>3252-3261</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>The transport of proteins at the cell surface of Bacteroidetes depends on a secretory apparatus known as type IX secretion system (T9SS). This machine is responsible for the cell surface exposition of various proteins, such as adhesins, required for gliding motility in
, S-layer components in
, and tooth tissue-degrading enzymes in the oral pathogen
Although a number of subunits of the T9SS have been identified, we lack details on the architecture of this secretion apparatus. Here we provide evidence that five of the genes encoding the core complex of the T9SS are co-transcribed and that the gene products are distributed in the cell envelope. Protein-protein interaction studies then revealed that these proteins oligomerize and interact through a dense network of contacts.</abstract><cop>United States</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>28057754</pmid><doi>10.1074/jbc.m116.765081</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-0611-9179</orcidid><orcidid>https://orcid.org/0000-0001-9106-9254</orcidid><orcidid>https://orcid.org/0000-0003-0425-5775</orcidid><orcidid>https://orcid.org/0000-0001-5502-4729</orcidid><orcidid>https://orcid.org/0000-0003-1902-3889</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Bacterial Proteins - analysis Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacterial Secretion Systems - analysis Bacterial Secretion Systems - genetics Bacterial Secretion Systems - metabolism Bacteriology Bacteroidaceae Infections - microbiology Biochemistry Biochemistry, Molecular Biology Biophysics Crystallography, X-Ray Genes, Bacterial Humans Life Sciences Microbiology Microbiology and Parasitology Molecular biology Molecular Networks Porphyromonas gingivalis - chemistry Porphyromonas gingivalis - genetics Porphyromonas gingivalis - metabolism Protein Interaction Maps Protein Subunits - analysis Protein Subunits - genetics Protein Subunits - metabolism Structural Biology |
| title | Characterization of the Porphyromonas gingivalis Type IX Secretion Trans-envelope PorKLMNP Core Complex |
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