In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration

A method for CRISPR-based genome editing that harnesses cellular non-homologous end joining activity to achieve targeted DNA knock-in in non-dividing tissues. A novel method for knock-in gene integration A current challenge in genome editing is achieving efficient targeted integration of transgenes...

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Veröffentlicht in:	Nature (London) 2016-12, Vol.540 (7631), p.144-149
Hauptverfasser:	Suzuki, Keiichiro, Tsunekawa, Yuji, Hernandez-Benitez, Reyna, Wu, Jun, Zhu, Jie, Kim, Euiseok J., Hatanaka, Fumiyuki, Yamamoto, Mako, Araoka, Toshikazu, Li, Zhe, Kurita, Masakazu, Hishida, Tomoaki, Li, Mo, Aizawa, Emi, Guo, Shicheng, Chen, Song, Goebl, April, Soligalla, Rupa Devi, Qu, Jing, Jiang, Tingshuai, Fu, Xin, Jafari, Maryam, Esteban, Concepcion Rodriguez, Berggren, W. Travis, Lajara, Jeronimo, Nuñez-Delicado, Estrella, Guillen, Pedro, Campistol, Josep M., Matsuzaki, Fumio, Liu, Guang-Hui, Magistretti, Pierre, Zhang, Kun, Callaway, Edward M., Zhang, Kang, Belmonte, Juan Carlos Izpisua
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/1513 631/208 Animals Cell Division CRISPR-Cas Systems - genetics Deoxyribonucleic acid Disease Models, Animal DNA Gene Editing - methods Gene Knock-In Techniques Gene targeting Gene Targeting - methods Gene therapy Genetic engineering Genetic Therapy - methods Genome - genetics Genomes Humanities and Social Sciences letter Methods multidisciplinary Neurons - cytology Neurons - metabolism Rats Retinitis Pigmentosa - genetics Retinitis Pigmentosa - therapy Science Sequence Homology Stem cells
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