Type B Chloramphenicol Acetyltransferases Are Responsible for Chloramphenicol Resistance in Riemerella anatipestifer , China
causes serositis and septicaemia in domestic ducks, geese, and turkeys. Traditionally, the antibiotics were used to treat this disease. Currently, our understanding of susceptibility to chloramphenicol and the underlying resistance mechanism is limited. In this study, the gene was identified in 69/1...
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| Veröffentlicht in: | Frontiers in microbiology 2017-03, Vol.8, p.297-297 |
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| creator | Huang, Li Yuan, Hui Liu, Ma-Feng Zhao, Xin-Xin Wang, Ming-Shu Jia, Ren-Yong Chen, Shun Sun, Kun-Feng Yang, Qiao Wu, Ying Chen, Xiao-Yue Cheng, An-Chun Zhu, De-Kang |
| description | causes serositis and septicaemia in domestic ducks, geese, and turkeys. Traditionally, the antibiotics were used to treat this disease. Currently, our understanding of
susceptibility to chloramphenicol and the underlying resistance mechanism is limited. In this study, the
gene was identified in 69/192 (36%)
isolated from different regions in China, including
CH-2 that has been sequenced in previous study. Sequence analysis suggested that there are two copies of
gene in this strain. Only both two copies of the
mutant strain showed a significant decrease in resistance to chloramphenicol, exhibiting 4 μg/ml in the minimum inhibitory concentration for this antibiotic, but not for the single
gene deletion strains. Functional analysis of the
gene via expression in
BL21 (DE3) cells and
site-directed mutagenesis indicated that His79 is the main catalytic residue of CAT in
. These results suggested that chloramphenicol resistance of
CH-2 is mediated by the
genes. Finally, homology analysis of types A and B CATs indicate that
comprises type B3 CATs. |
| doi_str_mv | 10.3389/fmicb.2017.00297 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5331189</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1878183028</sourcerecordid><originalsourceid>FETCH-LOGICAL-c396t-915ad6cd9493b85dbbb4d112bd5674357bdb8d43b624d880946f6813a77403ef3</originalsourceid><addsrcrecordid>eNplkUtrJCEUhSXMkISe7Gc1uMxiuqOlVaWbQKfJCwIDIQOzEx-30g5VWtHqQEN-fOy8yDAuVLj3nOvxQ-g7JQvGhDzpBm_NoiK0XRBSyXYPHdKm4XNGqj9fPt0P0FHOf0lZnFRl30cHlaikkKQ-RE932xHwGV6t-5j0MK4heBt7vLQwbfsp6ZA7SDpDxssE-BbyGEP2pgfcxfSfrNR9nnSwgH3Atx4GSND3GuugJz9Cnnyxwz-L0Af9DX3tdJ_h6O2cod8X53erq_nNr8vr1fJmbplsprmktXaNdZJLZkTtjDHcUVoZVzctZ3VrnBGOM9NU3AlBJG-6RlCm25YTBh2bodNX33FjBnAWQgnWqzH5QaetitqrfyvBr9V9fFQ1Y5QKWQyO3wxSfNiUFGrw2e6CBYibrKhoBRXls0VpJa-tNsWcE3QfYyhRO27qhZvacVMv3Irkx-fnfQjeKbFncKSXwg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1878183028</pqid></control><display><type>article</type><title>Type B Chloramphenicol Acetyltransferases Are Responsible for Chloramphenicol Resistance in Riemerella anatipestifer , China</title><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>PubMed Central Open Access</source><creator>Huang, Li ; Yuan, Hui ; Liu, Ma-Feng ; Zhao, Xin-Xin ; Wang, Ming-Shu ; Jia, Ren-Yong ; Chen, Shun ; Sun, Kun-Feng ; Yang, Qiao ; Wu, Ying ; Chen, Xiao-Yue ; Cheng, An-Chun ; Zhu, De-Kang</creator><creatorcontrib>Huang, Li ; Yuan, Hui ; Liu, Ma-Feng ; Zhao, Xin-Xin ; Wang, Ming-Shu ; Jia, Ren-Yong ; Chen, Shun ; Sun, Kun-Feng ; Yang, Qiao ; Wu, Ying ; Chen, Xiao-Yue ; Cheng, An-Chun ; Zhu, De-Kang</creatorcontrib><description>causes serositis and septicaemia in domestic ducks, geese, and turkeys. Traditionally, the antibiotics were used to treat this disease. Currently, our understanding of
susceptibility to chloramphenicol and the underlying resistance mechanism is limited. In this study, the
gene was identified in 69/192 (36%)
isolated from different regions in China, including
CH-2 that has been sequenced in previous study. Sequence analysis suggested that there are two copies of
gene in this strain. Only both two copies of the
mutant strain showed a significant decrease in resistance to chloramphenicol, exhibiting 4 μg/ml in the minimum inhibitory concentration for this antibiotic, but not for the single
gene deletion strains. Functional analysis of the
gene via expression in
BL21 (DE3) cells and
site-directed mutagenesis indicated that His79 is the main catalytic residue of CAT in
. These results suggested that chloramphenicol resistance of
CH-2 is mediated by the
genes. Finally, homology analysis of types A and B CATs indicate that
comprises type B3 CATs.</description><identifier>ISSN: 1664-302X</identifier><identifier>EISSN: 1664-302X</identifier><identifier>DOI: 10.3389/fmicb.2017.00297</identifier><identifier>PMID: 28298905</identifier><language>eng</language><publisher>Switzerland: Frontiers Media S.A</publisher><subject>Microbiology</subject><ispartof>Frontiers in microbiology, 2017-03, Vol.8, p.297-297</ispartof><rights>Copyright © 2017 Huang, Yuan, Liu, Zhao, Wang, Jia, Chen, Sun, Yang, Wu, Chen, Cheng and Zhu. 2017 Huang, Yuan, Liu, Zhao, Wang, Jia, Chen, Sun, Yang, Wu, Chen, Cheng and Zhu</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c396t-915ad6cd9493b85dbbb4d112bd5674357bdb8d43b624d880946f6813a77403ef3</citedby><cites>FETCH-LOGICAL-c396t-915ad6cd9493b85dbbb4d112bd5674357bdb8d43b624d880946f6813a77403ef3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5331189/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5331189/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28298905$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huang, Li</creatorcontrib><creatorcontrib>Yuan, Hui</creatorcontrib><creatorcontrib>Liu, Ma-Feng</creatorcontrib><creatorcontrib>Zhao, Xin-Xin</creatorcontrib><creatorcontrib>Wang, Ming-Shu</creatorcontrib><creatorcontrib>Jia, Ren-Yong</creatorcontrib><creatorcontrib>Chen, Shun</creatorcontrib><creatorcontrib>Sun, Kun-Feng</creatorcontrib><creatorcontrib>Yang, Qiao</creatorcontrib><creatorcontrib>Wu, Ying</creatorcontrib><creatorcontrib>Chen, Xiao-Yue</creatorcontrib><creatorcontrib>Cheng, An-Chun</creatorcontrib><creatorcontrib>Zhu, De-Kang</creatorcontrib><title>Type B Chloramphenicol Acetyltransferases Are Responsible for Chloramphenicol Resistance in Riemerella anatipestifer , China</title><title>Frontiers in microbiology</title><addtitle>Front Microbiol</addtitle><description>causes serositis and septicaemia in domestic ducks, geese, and turkeys. Traditionally, the antibiotics were used to treat this disease. Currently, our understanding of
susceptibility to chloramphenicol and the underlying resistance mechanism is limited. In this study, the
gene was identified in 69/192 (36%)
isolated from different regions in China, including
CH-2 that has been sequenced in previous study. Sequence analysis suggested that there are two copies of
gene in this strain. Only both two copies of the
mutant strain showed a significant decrease in resistance to chloramphenicol, exhibiting 4 μg/ml in the minimum inhibitory concentration for this antibiotic, but not for the single
gene deletion strains. Functional analysis of the
gene via expression in
BL21 (DE3) cells and
site-directed mutagenesis indicated that His79 is the main catalytic residue of CAT in
. These results suggested that chloramphenicol resistance of
CH-2 is mediated by the
genes. Finally, homology analysis of types A and B CATs indicate that
comprises type B3 CATs.</description><subject>Microbiology</subject><issn>1664-302X</issn><issn>1664-302X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNplkUtrJCEUhSXMkISe7Gc1uMxiuqOlVaWbQKfJCwIDIQOzEx-30g5VWtHqQEN-fOy8yDAuVLj3nOvxQ-g7JQvGhDzpBm_NoiK0XRBSyXYPHdKm4XNGqj9fPt0P0FHOf0lZnFRl30cHlaikkKQ-RE932xHwGV6t-5j0MK4heBt7vLQwbfsp6ZA7SDpDxssE-BbyGEP2pgfcxfSfrNR9nnSwgH3Atx4GSND3GuugJz9Cnnyxwz-L0Af9DX3tdJ_h6O2cod8X53erq_nNr8vr1fJmbplsprmktXaNdZJLZkTtjDHcUVoZVzctZ3VrnBGOM9NU3AlBJG-6RlCm25YTBh2bodNX33FjBnAWQgnWqzH5QaetitqrfyvBr9V9fFQ1Y5QKWQyO3wxSfNiUFGrw2e6CBYibrKhoBRXls0VpJa-tNsWcE3QfYyhRO27qhZvacVMv3Irkx-fnfQjeKbFncKSXwg</recordid><startdate>20170301</startdate><enddate>20170301</enddate><creator>Huang, Li</creator><creator>Yuan, Hui</creator><creator>Liu, Ma-Feng</creator><creator>Zhao, Xin-Xin</creator><creator>Wang, Ming-Shu</creator><creator>Jia, Ren-Yong</creator><creator>Chen, Shun</creator><creator>Sun, Kun-Feng</creator><creator>Yang, Qiao</creator><creator>Wu, Ying</creator><creator>Chen, Xiao-Yue</creator><creator>Cheng, An-Chun</creator><creator>Zhu, De-Kang</creator><general>Frontiers Media S.A</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20170301</creationdate><title>Type B Chloramphenicol Acetyltransferases Are Responsible for Chloramphenicol Resistance in Riemerella anatipestifer , China</title><author>Huang, Li ; Yuan, Hui ; Liu, Ma-Feng ; Zhao, Xin-Xin ; Wang, Ming-Shu ; Jia, Ren-Yong ; Chen, Shun ; Sun, Kun-Feng ; Yang, Qiao ; Wu, Ying ; Chen, Xiao-Yue ; Cheng, An-Chun ; Zhu, De-Kang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c396t-915ad6cd9493b85dbbb4d112bd5674357bdb8d43b624d880946f6813a77403ef3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huang, Li</creatorcontrib><creatorcontrib>Yuan, Hui</creatorcontrib><creatorcontrib>Liu, Ma-Feng</creatorcontrib><creatorcontrib>Zhao, Xin-Xin</creatorcontrib><creatorcontrib>Wang, Ming-Shu</creatorcontrib><creatorcontrib>Jia, Ren-Yong</creatorcontrib><creatorcontrib>Chen, Shun</creatorcontrib><creatorcontrib>Sun, Kun-Feng</creatorcontrib><creatorcontrib>Yang, Qiao</creatorcontrib><creatorcontrib>Wu, Ying</creatorcontrib><creatorcontrib>Chen, Xiao-Yue</creatorcontrib><creatorcontrib>Cheng, An-Chun</creatorcontrib><creatorcontrib>Zhu, De-Kang</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Frontiers in microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, Li</au><au>Yuan, Hui</au><au>Liu, Ma-Feng</au><au>Zhao, Xin-Xin</au><au>Wang, Ming-Shu</au><au>Jia, Ren-Yong</au><au>Chen, Shun</au><au>Sun, Kun-Feng</au><au>Yang, Qiao</au><au>Wu, Ying</au><au>Chen, Xiao-Yue</au><au>Cheng, An-Chun</au><au>Zhu, De-Kang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Type B Chloramphenicol Acetyltransferases Are Responsible for Chloramphenicol Resistance in Riemerella anatipestifer , China</atitle><jtitle>Frontiers in microbiology</jtitle><addtitle>Front Microbiol</addtitle><date>2017-03-01</date><risdate>2017</risdate><volume>8</volume><spage>297</spage><epage>297</epage><pages>297-297</pages><issn>1664-302X</issn><eissn>1664-302X</eissn><abstract>causes serositis and septicaemia in domestic ducks, geese, and turkeys. Traditionally, the antibiotics were used to treat this disease. Currently, our understanding of
susceptibility to chloramphenicol and the underlying resistance mechanism is limited. In this study, the
gene was identified in 69/192 (36%)
isolated from different regions in China, including
CH-2 that has been sequenced in previous study. Sequence analysis suggested that there are two copies of
gene in this strain. Only both two copies of the
mutant strain showed a significant decrease in resistance to chloramphenicol, exhibiting 4 μg/ml in the minimum inhibitory concentration for this antibiotic, but not for the single
gene deletion strains. Functional analysis of the
gene via expression in
BL21 (DE3) cells and
site-directed mutagenesis indicated that His79 is the main catalytic residue of CAT in
. These results suggested that chloramphenicol resistance of
CH-2 is mediated by the
genes. Finally, homology analysis of types A and B CATs indicate that
comprises type B3 CATs.</abstract><cop>Switzerland</cop><pub>Frontiers Media S.A</pub><pmid>28298905</pmid><doi>10.3389/fmicb.2017.00297</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Microbiology |
| title | Type B Chloramphenicol Acetyltransferases Are Responsible for Chloramphenicol Resistance in Riemerella anatipestifer , China |
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