Heart extracellular matrix supports cardiomyocyte differentiation of mouse embryonic stem cells
We have evaluated the effect of heart extracellular matrix (ECM) on the cardiomyocyte differentiation of mouse embryonic stem cells (ES cells) using de-cellularized heart tissue. Several lines of evidence indicate that ECM plays significant roles in cell proliferation, cell death and differentiation...
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| Veröffentlicht in: | Journal of bioscience and bioengineering 2013-03, Vol.115 (3), p.320-325 |
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| creator | Higuchi, Sayaka Lin, Qingsong Wang, Jigang Lim, Teck Kwang Joshi, Shashikant B. Anand, Ganesh Srinivasan Chung, Maxey C.M. Sheetz, Michael P. Fujita, Hideaki |
| description | We have evaluated the effect of heart extracellular matrix (ECM) on the cardiomyocyte differentiation of mouse embryonic stem cells (ES cells) using de-cellularized heart tissue. Several lines of evidence indicate that ECM plays significant roles in cell proliferation, cell death and differentiation, but role of ECM possessing a 3D structure in differentiation has not been studied in detail. We found that there are substantial differences in the quantitative protein profiles of ECM in SDS-treated heart tissue compared to that of liver tissue, as assessed by iTRAQ™ quantitative proteomics analysis. When mouse ES cells were cultured on thin (60 μm) sections of de-cellularized tissue, the expression of cardiac myosin heavy chain (cMHC) and cardiac troponin I (cTnI) was high in ES cells cultured on heart ECM compared with those cultured on liver ECM. In addition, the protein expression of cMHC and cTnI was detected in cells on heart ECM after 2 weeks, which was not detectable in cells on liver ECM. These results indicate that heart ECM plays a critical role in the cardiomyocyte differentiation of ES cells. We propose that tissue-specific ECM induced cell lineage specification through mechano-transduction mediated by the structure, elasticity and components of ECM. |
| doi_str_mv | 10.1016/j.jbiosc.2012.10.004 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5330950</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1389172312004136</els_id><sourcerecordid>2000103870</sourcerecordid><originalsourceid>FETCH-LOGICAL-c673t-afb1a4fb154755e3f7b5539e477aaf0d7493c458aeb8abe209aceedd0c4e6883</originalsourceid><addsrcrecordid>eNqNkU9v1DAQxSMEoqXwDRDkgsQli__GzgUJVUCRKnGgnK2JMy5eJfFiO1X32-OwSwsX4GJb45-f38yrqueUbCih7ZvtZtv7kOyGEcpKaUOIeFCdUi5UIwSjD9ez7hqqGD-pnqS0JYQqoujj6oRx2mqu-WllLhBirvE2R7A4jssIsZ4gR39bp2W3CzGn2kIcfJj2we4z1oN3DiPO2UP2Ya6Dq6ewJKxx6uM-zN7WKeNUr3LpafXIwZjw2XE_q64-vL86v2guP3_8dP7usrGt4rkB11MQZZFCSYncqV5K3qFQCsCRQYmOWyE1YK-hR0a64haHgViBrdb8rHp7kN0t_YSDLe4ijGYX_QRxbwJ48-fN7L-Z63BjJOekk6QIvD4KxPB9wZTN5NPaAcxYmjOMlOkRrtW_Ucq0XFHxP6jikrKWdQUVB9TGkFJEd2eeErMGbrbmELhZA1-rJfDy7MXvjd89-pVwAV4dAUgWRhdhtj7dc4rRrtOycC8PnINg4DoW5uuX8lP7s3Gm2_sZY8nxxmM0yXqcLQ4-os1mCP7vXn8A3THXVg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1273512629</pqid></control><display><type>article</type><title>Heart extracellular matrix supports cardiomyocyte differentiation of mouse embryonic stem cells</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Higuchi, Sayaka ; Lin, Qingsong ; Wang, Jigang ; Lim, Teck Kwang ; Joshi, Shashikant B. ; Anand, Ganesh Srinivasan ; Chung, Maxey C.M. ; Sheetz, Michael P. ; Fujita, Hideaki</creator><creatorcontrib>Higuchi, Sayaka ; Lin, Qingsong ; Wang, Jigang ; Lim, Teck Kwang ; Joshi, Shashikant B. ; Anand, Ganesh Srinivasan ; Chung, Maxey C.M. ; Sheetz, Michael P. ; Fujita, Hideaki</creatorcontrib><description>We have evaluated the effect of heart extracellular matrix (ECM) on the cardiomyocyte differentiation of mouse embryonic stem cells (ES cells) using de-cellularized heart tissue. Several lines of evidence indicate that ECM plays significant roles in cell proliferation, cell death and differentiation, but role of ECM possessing a 3D structure in differentiation has not been studied in detail. We found that there are substantial differences in the quantitative protein profiles of ECM in SDS-treated heart tissue compared to that of liver tissue, as assessed by iTRAQ™ quantitative proteomics analysis. When mouse ES cells were cultured on thin (60 μm) sections of de-cellularized tissue, the expression of cardiac myosin heavy chain (cMHC) and cardiac troponin I (cTnI) was high in ES cells cultured on heart ECM compared with those cultured on liver ECM. In addition, the protein expression of cMHC and cTnI was detected in cells on heart ECM after 2 weeks, which was not detectable in cells on liver ECM. These results indicate that heart ECM plays a critical role in the cardiomyocyte differentiation of ES cells. We propose that tissue-specific ECM induced cell lineage specification through mechano-transduction mediated by the structure, elasticity and components of ECM.</description><identifier>ISSN: 1389-1723</identifier><identifier>EISSN: 1347-4421</identifier><identifier>DOI: 10.1016/j.jbiosc.2012.10.004</identifier><identifier>PMID: 23168383</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Biological and medical sciences ; Biotechnology ; Cardiomyocyte ; cardiomyocytes ; Cell death ; Cell Differentiation ; cell proliferation ; Cells, Cultured ; cultured cells ; Differentiation ; Embryonic stem cells ; Embryonic Stem Cells - cytology ; Embryonic Stem Cells - metabolism ; Extracellular matrix ; Extracellular Matrix Proteins - analysis ; Extracellular Matrix Proteins - physiology ; Fundamental and applied biological sciences. Psychology ; hepatocytes ; liver ; Liver - chemistry ; Mechanobiology ; Mice ; Myocardium - chemistry ; Myocytes, Cardiac - cytology ; Myocytes, Cardiac - metabolism ; myosin heavy chains ; protein synthesis ; proteomics ; tissue ; troponin I</subject><ispartof>Journal of bioscience and bioengineering, 2013-03, Vol.115 (3), p.320-325</ispartof><rights>2012 The Society for Biotechnology, Japan</rights><rights>2014 INIST-CNRS</rights><rights>Copyright © 2012 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c673t-afb1a4fb154755e3f7b5539e477aaf0d7493c458aeb8abe209aceedd0c4e6883</citedby><cites>FETCH-LOGICAL-c673t-afb1a4fb154755e3f7b5539e477aaf0d7493c458aeb8abe209aceedd0c4e6883</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1389172312004136$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27219985$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23168383$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Higuchi, Sayaka</creatorcontrib><creatorcontrib>Lin, Qingsong</creatorcontrib><creatorcontrib>Wang, Jigang</creatorcontrib><creatorcontrib>Lim, Teck Kwang</creatorcontrib><creatorcontrib>Joshi, Shashikant B.</creatorcontrib><creatorcontrib>Anand, Ganesh Srinivasan</creatorcontrib><creatorcontrib>Chung, Maxey C.M.</creatorcontrib><creatorcontrib>Sheetz, Michael P.</creatorcontrib><creatorcontrib>Fujita, Hideaki</creatorcontrib><title>Heart extracellular matrix supports cardiomyocyte differentiation of mouse embryonic stem cells</title><title>Journal of bioscience and bioengineering</title><addtitle>J Biosci Bioeng</addtitle><description>We have evaluated the effect of heart extracellular matrix (ECM) on the cardiomyocyte differentiation of mouse embryonic stem cells (ES cells) using de-cellularized heart tissue. Several lines of evidence indicate that ECM plays significant roles in cell proliferation, cell death and differentiation, but role of ECM possessing a 3D structure in differentiation has not been studied in detail. We found that there are substantial differences in the quantitative protein profiles of ECM in SDS-treated heart tissue compared to that of liver tissue, as assessed by iTRAQ™ quantitative proteomics analysis. When mouse ES cells were cultured on thin (60 μm) sections of de-cellularized tissue, the expression of cardiac myosin heavy chain (cMHC) and cardiac troponin I (cTnI) was high in ES cells cultured on heart ECM compared with those cultured on liver ECM. In addition, the protein expression of cMHC and cTnI was detected in cells on heart ECM after 2 weeks, which was not detectable in cells on liver ECM. These results indicate that heart ECM plays a critical role in the cardiomyocyte differentiation of ES cells. We propose that tissue-specific ECM induced cell lineage specification through mechano-transduction mediated by the structure, elasticity and components of ECM.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cardiomyocyte</subject><subject>cardiomyocytes</subject><subject>Cell death</subject><subject>Cell Differentiation</subject><subject>cell proliferation</subject><subject>Cells, Cultured</subject><subject>cultured cells</subject><subject>Differentiation</subject><subject>Embryonic stem cells</subject><subject>Embryonic Stem Cells - cytology</subject><subject>Embryonic Stem Cells - metabolism</subject><subject>Extracellular matrix</subject><subject>Extracellular Matrix Proteins - analysis</subject><subject>Extracellular Matrix Proteins - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>hepatocytes</subject><subject>liver</subject><subject>Liver - chemistry</subject><subject>Mechanobiology</subject><subject>Mice</subject><subject>Myocardium - chemistry</subject><subject>Myocytes, Cardiac - cytology</subject><subject>Myocytes, Cardiac - metabolism</subject><subject>myosin heavy chains</subject><subject>protein synthesis</subject><subject>proteomics</subject><subject>tissue</subject><subject>troponin I</subject><issn>1389-1723</issn><issn>1347-4421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU9v1DAQxSMEoqXwDRDkgsQli__GzgUJVUCRKnGgnK2JMy5eJfFiO1X32-OwSwsX4GJb45-f38yrqueUbCih7ZvtZtv7kOyGEcpKaUOIeFCdUi5UIwSjD9ez7hqqGD-pnqS0JYQqoujj6oRx2mqu-WllLhBirvE2R7A4jssIsZ4gR39bp2W3CzGn2kIcfJj2we4z1oN3DiPO2UP2Ya6Dq6ewJKxx6uM-zN7WKeNUr3LpafXIwZjw2XE_q64-vL86v2guP3_8dP7usrGt4rkB11MQZZFCSYncqV5K3qFQCsCRQYmOWyE1YK-hR0a64haHgViBrdb8rHp7kN0t_YSDLe4ijGYX_QRxbwJ48-fN7L-Z63BjJOekk6QIvD4KxPB9wZTN5NPaAcxYmjOMlOkRrtW_Ucq0XFHxP6jikrKWdQUVB9TGkFJEd2eeErMGbrbmELhZA1-rJfDy7MXvjd89-pVwAV4dAUgWRhdhtj7dc4rRrtOycC8PnINg4DoW5uuX8lP7s3Gm2_sZY8nxxmM0yXqcLQ4-os1mCP7vXn8A3THXVg</recordid><startdate>20130301</startdate><enddate>20130301</enddate><creator>Higuchi, Sayaka</creator><creator>Lin, Qingsong</creator><creator>Wang, Jigang</creator><creator>Lim, Teck Kwang</creator><creator>Joshi, Shashikant B.</creator><creator>Anand, Ganesh Srinivasan</creator><creator>Chung, Maxey C.M.</creator><creator>Sheetz, Michael P.</creator><creator>Fujita, Hideaki</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope></search><sort><creationdate>20130301</creationdate><title>Heart extracellular matrix supports cardiomyocyte differentiation of mouse embryonic stem cells</title><author>Higuchi, Sayaka ; Lin, Qingsong ; Wang, Jigang ; Lim, Teck Kwang ; Joshi, Shashikant B. ; Anand, Ganesh Srinivasan ; Chung, Maxey C.M. ; Sheetz, Michael P. ; Fujita, Hideaki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c673t-afb1a4fb154755e3f7b5539e477aaf0d7493c458aeb8abe209aceedd0c4e6883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cardiomyocyte</topic><topic>cardiomyocytes</topic><topic>Cell death</topic><topic>Cell Differentiation</topic><topic>cell proliferation</topic><topic>Cells, Cultured</topic><topic>cultured cells</topic><topic>Differentiation</topic><topic>Embryonic stem cells</topic><topic>Embryonic Stem Cells - cytology</topic><topic>Embryonic Stem Cells - metabolism</topic><topic>Extracellular matrix</topic><topic>Extracellular Matrix Proteins - analysis</topic><topic>Extracellular Matrix Proteins - physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>hepatocytes</topic><topic>liver</topic><topic>Liver - chemistry</topic><topic>Mechanobiology</topic><topic>Mice</topic><topic>Myocardium - chemistry</topic><topic>Myocytes, Cardiac - cytology</topic><topic>Myocytes, Cardiac - metabolism</topic><topic>myosin heavy chains</topic><topic>protein synthesis</topic><topic>proteomics</topic><topic>tissue</topic><topic>troponin I</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Higuchi, Sayaka</creatorcontrib><creatorcontrib>Lin, Qingsong</creatorcontrib><creatorcontrib>Wang, Jigang</creatorcontrib><creatorcontrib>Lim, Teck Kwang</creatorcontrib><creatorcontrib>Joshi, Shashikant B.</creatorcontrib><creatorcontrib>Anand, Ganesh Srinivasan</creatorcontrib><creatorcontrib>Chung, Maxey C.M.</creatorcontrib><creatorcontrib>Sheetz, Michael P.</creatorcontrib><creatorcontrib>Fujita, Hideaki</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of bioscience and bioengineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Higuchi, Sayaka</au><au>Lin, Qingsong</au><au>Wang, Jigang</au><au>Lim, Teck Kwang</au><au>Joshi, Shashikant B.</au><au>Anand, Ganesh Srinivasan</au><au>Chung, Maxey C.M.</au><au>Sheetz, Michael P.</au><au>Fujita, Hideaki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Heart extracellular matrix supports cardiomyocyte differentiation of mouse embryonic stem cells</atitle><jtitle>Journal of bioscience and bioengineering</jtitle><addtitle>J Biosci Bioeng</addtitle><date>2013-03-01</date><risdate>2013</risdate><volume>115</volume><issue>3</issue><spage>320</spage><epage>325</epage><pages>320-325</pages><issn>1389-1723</issn><eissn>1347-4421</eissn><abstract>We have evaluated the effect of heart extracellular matrix (ECM) on the cardiomyocyte differentiation of mouse embryonic stem cells (ES cells) using de-cellularized heart tissue. Several lines of evidence indicate that ECM plays significant roles in cell proliferation, cell death and differentiation, but role of ECM possessing a 3D structure in differentiation has not been studied in detail. We found that there are substantial differences in the quantitative protein profiles of ECM in SDS-treated heart tissue compared to that of liver tissue, as assessed by iTRAQ™ quantitative proteomics analysis. When mouse ES cells were cultured on thin (60 μm) sections of de-cellularized tissue, the expression of cardiac myosin heavy chain (cMHC) and cardiac troponin I (cTnI) was high in ES cells cultured on heart ECM compared with those cultured on liver ECM. In addition, the protein expression of cMHC and cTnI was detected in cells on heart ECM after 2 weeks, which was not detectable in cells on liver ECM. These results indicate that heart ECM plays a critical role in the cardiomyocyte differentiation of ES cells. We propose that tissue-specific ECM induced cell lineage specification through mechano-transduction mediated by the structure, elasticity and components of ECM.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>23168383</pmid><doi>10.1016/j.jbiosc.2012.10.004</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of bioscience and bioengineering, 2013-03, Vol.115 (3), p.320-325 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Biological and medical sciences Biotechnology Cardiomyocyte cardiomyocytes Cell death Cell Differentiation cell proliferation Cells, Cultured cultured cells Differentiation Embryonic stem cells Embryonic Stem Cells - cytology Embryonic Stem Cells - metabolism Extracellular matrix Extracellular Matrix Proteins - analysis Extracellular Matrix Proteins - physiology Fundamental and applied biological sciences. Psychology hepatocytes liver Liver - chemistry Mechanobiology Mice Myocardium - chemistry Myocytes, Cardiac - cytology Myocytes, Cardiac - metabolism myosin heavy chains protein synthesis proteomics tissue troponin I |
| title | Heart extracellular matrix supports cardiomyocyte differentiation of mouse embryonic stem cells |
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