The Hydroalcoholic Extract of Matricaria chamomilla Suppresses Migration and Invasion of Human Breast Cancer MDA-MB-468 and MCF-7 Cell Lines
is an aromatic plant with antioxidant, anticancer, and anti-inflammatory properties. However, the inhibitory role of on migration and invasion of human breast cancer cells remains unclear. This study investigated the methods to evaluate these anticancer mechanisms of on human breast cancer MCF-7 and...
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| Veröffentlicht in: | Pharmacognosy research 2017-01, Vol.9 (1), p.87-95 |
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| creator | Nikseresht, Mohsen Kamali, Ali Mohammad Rahimi, Hamid Reza Delaviz, Hamdollah Toori, Mehdi Akbartabar Kashani, Iraj Ragerdi Mahmoudi, Reza |
| description | is an aromatic plant with antioxidant, anticancer, and anti-inflammatory properties. However, the inhibitory role of
on migration and invasion of human breast cancer cells remains unclear.
This study investigated the methods to evaluate these anticancer mechanisms of
on human breast cancer MCF-7 and MDA-MB-468 cell lines.
The cells were treated with hydroalcoholic extract of
at different concentrations (50-1300 μg/mL) for 24, 48, and 72 h in a culture medium containing 10% fetal bovine serum. This study quantified the 50% growth inhibition concentrations (IC
) by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay; apoptosis and necrosis through Hoechst 33342/propidium iodide staining; cell proliferation and clone formation by clonogenic assay as well as cellular migration, invasion, and attachment. After 24, 48, and 72 h of treatment, the IC
levels were 992 ± 2.3 μg/mL, 893 ± 5.4 μg/mL, and 785 ± 4.8 μg/mL against MDA-MB-468, respectively, and 1288 ± 5.6 μg/mL, 926 ± 2.5 μg/mL, and 921 ± 3.5 μg/mL, against MCF-7, respectively. Furthermore, increasing the extract concentrations induced cellular apoptosis and necrosis and decreased cellular invasion or migration through 8 μm pores, colonization and attachment in a dose-dependent manner.
It indicated time- and dose-dependent anti-invasive and antimigrative or proliferative and antitoxic effects of hydroalcoholic extract of aerial parts of chamomile on breast cancer cells.
This study demonstrated an effective plant in preventing or treating breast cancer.
Antioxidant compounds in
have anticancer effects.Hydroalcoholic extract of
controls cellular proliferation and apoptosis induction.Hoechst 33342/propidium iodide staining suggested that the extract induces apoptosis more than necrosis.Hydroalcoholic extract of
prevents colonization and cellular migration of human breast cancer MDA-MB-468 and MCF-7 cell lines in a time- and dose-dependent manner.
has low cytotoxic effects on natural cells.
: IARC: International Agency for Research on Cancer; WHO: World Health Organization; FBS: Fetal bovine serum; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; DMSO: Dimethyl sulfoxide; PI: Propidium iodide; LN: Live cells with normal nucleus; LA: Live cells with apoptized nucleus; DN: Dead cells with normal nucleus; DA: Dead cells with apoptized nucleus; BSA: Bovine serum albumin; ANOVA: Analysis of variance; IC
: 50% growth inhibition concentration; GSE: Grape seed extract. |
| doi_str_mv | 10.4103/0974-8490.199778 |
| format | Article |
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on migration and invasion of human breast cancer cells remains unclear.
This study investigated the methods to evaluate these anticancer mechanisms of
on human breast cancer MCF-7 and MDA-MB-468 cell lines.
The cells were treated with hydroalcoholic extract of
at different concentrations (50-1300 μg/mL) for 24, 48, and 72 h in a culture medium containing 10% fetal bovine serum. This study quantified the 50% growth inhibition concentrations (IC
) by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay; apoptosis and necrosis through Hoechst 33342/propidium iodide staining; cell proliferation and clone formation by clonogenic assay as well as cellular migration, invasion, and attachment. After 24, 48, and 72 h of treatment, the IC
levels were 992 ± 2.3 μg/mL, 893 ± 5.4 μg/mL, and 785 ± 4.8 μg/mL against MDA-MB-468, respectively, and 1288 ± 5.6 μg/mL, 926 ± 2.5 μg/mL, and 921 ± 3.5 μg/mL, against MCF-7, respectively. Furthermore, increasing the extract concentrations induced cellular apoptosis and necrosis and decreased cellular invasion or migration through 8 μm pores, colonization and attachment in a dose-dependent manner.
It indicated time- and dose-dependent anti-invasive and antimigrative or proliferative and antitoxic effects of hydroalcoholic extract of aerial parts of chamomile on breast cancer cells.
This study demonstrated an effective plant in preventing or treating breast cancer.
Antioxidant compounds in
have anticancer effects.Hydroalcoholic extract of
controls cellular proliferation and apoptosis induction.Hoechst 33342/propidium iodide staining suggested that the extract induces apoptosis more than necrosis.Hydroalcoholic extract of
prevents colonization and cellular migration of human breast cancer MDA-MB-468 and MCF-7 cell lines in a time- and dose-dependent manner.
has low cytotoxic effects on natural cells.
: IARC: International Agency for Research on Cancer; WHO: World Health Organization; FBS: Fetal bovine serum; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; DMSO: Dimethyl sulfoxide; PI: Propidium iodide; LN: Live cells with normal nucleus; LA: Live cells with apoptized nucleus; DN: Dead cells with normal nucleus; DA: Dead cells with apoptized nucleus; BSA: Bovine serum albumin; ANOVA: Analysis of variance; IC
: 50% growth inhibition concentration; GSE: Grape seed extract.</description><identifier>ISSN: 0976-4836</identifier><identifier>ISSN: 0974-8490</identifier><identifier>EISSN: 0974-8490</identifier><identifier>DOI: 10.4103/0974-8490.199778</identifier><identifier>PMID: 28250660</identifier><language>eng</language><publisher>India: Medknow Publications & Media Pvt Ltd</publisher><subject>Original</subject><ispartof>Pharmacognosy research, 2017-01, Vol.9 (1), p.87-95</ispartof><rights>Copyright: © 2017 Pharmacognosy Research 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c396t-6d9369004dd201b73624270fffe360773937d20b4f667963338f40c039cbb4a73</citedby><cites>FETCH-LOGICAL-c396t-6d9369004dd201b73624270fffe360773937d20b4f667963338f40c039cbb4a73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5330110/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5330110/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,882,4011,27905,27906,27907,53773,53775</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28250660$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nikseresht, Mohsen</creatorcontrib><creatorcontrib>Kamali, Ali Mohammad</creatorcontrib><creatorcontrib>Rahimi, Hamid Reza</creatorcontrib><creatorcontrib>Delaviz, Hamdollah</creatorcontrib><creatorcontrib>Toori, Mehdi Akbartabar</creatorcontrib><creatorcontrib>Kashani, Iraj Ragerdi</creatorcontrib><creatorcontrib>Mahmoudi, Reza</creatorcontrib><title>The Hydroalcoholic Extract of Matricaria chamomilla Suppresses Migration and Invasion of Human Breast Cancer MDA-MB-468 and MCF-7 Cell Lines</title><title>Pharmacognosy research</title><addtitle>Pharmacognosy Res</addtitle><description>is an aromatic plant with antioxidant, anticancer, and anti-inflammatory properties. However, the inhibitory role of
on migration and invasion of human breast cancer cells remains unclear.
This study investigated the methods to evaluate these anticancer mechanisms of
on human breast cancer MCF-7 and MDA-MB-468 cell lines.
The cells were treated with hydroalcoholic extract of
at different concentrations (50-1300 μg/mL) for 24, 48, and 72 h in a culture medium containing 10% fetal bovine serum. This study quantified the 50% growth inhibition concentrations (IC
) by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay; apoptosis and necrosis through Hoechst 33342/propidium iodide staining; cell proliferation and clone formation by clonogenic assay as well as cellular migration, invasion, and attachment. After 24, 48, and 72 h of treatment, the IC
levels were 992 ± 2.3 μg/mL, 893 ± 5.4 μg/mL, and 785 ± 4.8 μg/mL against MDA-MB-468, respectively, and 1288 ± 5.6 μg/mL, 926 ± 2.5 μg/mL, and 921 ± 3.5 μg/mL, against MCF-7, respectively. Furthermore, increasing the extract concentrations induced cellular apoptosis and necrosis and decreased cellular invasion or migration through 8 μm pores, colonization and attachment in a dose-dependent manner.
It indicated time- and dose-dependent anti-invasive and antimigrative or proliferative and antitoxic effects of hydroalcoholic extract of aerial parts of chamomile on breast cancer cells.
This study demonstrated an effective plant in preventing or treating breast cancer.
Antioxidant compounds in
have anticancer effects.Hydroalcoholic extract of
controls cellular proliferation and apoptosis induction.Hoechst 33342/propidium iodide staining suggested that the extract induces apoptosis more than necrosis.Hydroalcoholic extract of
prevents colonization and cellular migration of human breast cancer MDA-MB-468 and MCF-7 cell lines in a time- and dose-dependent manner.
has low cytotoxic effects on natural cells.
: IARC: International Agency for Research on Cancer; WHO: World Health Organization; FBS: Fetal bovine serum; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; DMSO: Dimethyl sulfoxide; PI: Propidium iodide; LN: Live cells with normal nucleus; LA: Live cells with apoptized nucleus; DN: Dead cells with normal nucleus; DA: Dead cells with apoptized nucleus; BSA: Bovine serum albumin; ANOVA: Analysis of variance; IC
: 50% growth inhibition concentration; GSE: Grape seed extract.</description><subject>Original</subject><issn>0976-4836</issn><issn>0974-8490</issn><issn>0974-8490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNpVkc1u1DAUhS0EotXQPSvkJZsUO9e14w1Sm7ZMpYlYUNbWjeN0jBJ7sJOKvgMPTaZTRuCNf-451z7-CHnP2bngDD4xrURRCc3OudZKVa_I6fHo9fNaFqICeULOcv7BlgG61IK_JSdlVV4wKdkp-X2_dXT91KWIg43bOHhLb35NCe1EY08bnJK3mDxSu8Uxjn4YkH6bd7vkcnaZNv4h4eRjoBg6ehceMe83i3U9jxjoVXKYJ1pjsC7R5vqyaK4KIatneVPfForWbhjoxgeX35E3PQ7Znb3MK_L99ua-Xhebr1_u6stNYUHLqZCdBqkZE11XMt4qkKUoFev73oFkSoEGtVRa0UuptASAqhfMLvFt2wpUsCKfD313czu6zrqwBB7MLvkR05OJ6M3_leC35iE-mgsAxpe_X5GPLw1S_Dm7PJnRZ7vkwODinA2vFKhScA2LlB2kNsWck-uP13Bm9hzNHprZQzMHjovlw7_POxr-UoM_882XSg</recordid><startdate>201701</startdate><enddate>201701</enddate><creator>Nikseresht, Mohsen</creator><creator>Kamali, Ali Mohammad</creator><creator>Rahimi, Hamid Reza</creator><creator>Delaviz, Hamdollah</creator><creator>Toori, Mehdi Akbartabar</creator><creator>Kashani, Iraj Ragerdi</creator><creator>Mahmoudi, Reza</creator><general>Medknow Publications & Media Pvt Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>201701</creationdate><title>The Hydroalcoholic Extract of Matricaria chamomilla Suppresses Migration and Invasion of Human Breast Cancer MDA-MB-468 and MCF-7 Cell Lines</title><author>Nikseresht, Mohsen ; Kamali, Ali Mohammad ; Rahimi, Hamid Reza ; Delaviz, Hamdollah ; Toori, Mehdi Akbartabar ; Kashani, Iraj Ragerdi ; Mahmoudi, Reza</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c396t-6d9369004dd201b73624270fffe360773937d20b4f667963338f40c039cbb4a73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Original</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nikseresht, Mohsen</creatorcontrib><creatorcontrib>Kamali, Ali Mohammad</creatorcontrib><creatorcontrib>Rahimi, Hamid Reza</creatorcontrib><creatorcontrib>Delaviz, Hamdollah</creatorcontrib><creatorcontrib>Toori, Mehdi Akbartabar</creatorcontrib><creatorcontrib>Kashani, Iraj Ragerdi</creatorcontrib><creatorcontrib>Mahmoudi, Reza</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Pharmacognosy research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nikseresht, Mohsen</au><au>Kamali, Ali Mohammad</au><au>Rahimi, Hamid Reza</au><au>Delaviz, Hamdollah</au><au>Toori, Mehdi Akbartabar</au><au>Kashani, Iraj Ragerdi</au><au>Mahmoudi, Reza</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Hydroalcoholic Extract of Matricaria chamomilla Suppresses Migration and Invasion of Human Breast Cancer MDA-MB-468 and MCF-7 Cell Lines</atitle><jtitle>Pharmacognosy research</jtitle><addtitle>Pharmacognosy Res</addtitle><date>2017-01</date><risdate>2017</risdate><volume>9</volume><issue>1</issue><spage>87</spage><epage>95</epage><pages>87-95</pages><issn>0976-4836</issn><issn>0974-8490</issn><eissn>0974-8490</eissn><abstract>is an aromatic plant with antioxidant, anticancer, and anti-inflammatory properties. However, the inhibitory role of
on migration and invasion of human breast cancer cells remains unclear.
This study investigated the methods to evaluate these anticancer mechanisms of
on human breast cancer MCF-7 and MDA-MB-468 cell lines.
The cells were treated with hydroalcoholic extract of
at different concentrations (50-1300 μg/mL) for 24, 48, and 72 h in a culture medium containing 10% fetal bovine serum. This study quantified the 50% growth inhibition concentrations (IC
) by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay; apoptosis and necrosis through Hoechst 33342/propidium iodide staining; cell proliferation and clone formation by clonogenic assay as well as cellular migration, invasion, and attachment. After 24, 48, and 72 h of treatment, the IC
levels were 992 ± 2.3 μg/mL, 893 ± 5.4 μg/mL, and 785 ± 4.8 μg/mL against MDA-MB-468, respectively, and 1288 ± 5.6 μg/mL, 926 ± 2.5 μg/mL, and 921 ± 3.5 μg/mL, against MCF-7, respectively. Furthermore, increasing the extract concentrations induced cellular apoptosis and necrosis and decreased cellular invasion or migration through 8 μm pores, colonization and attachment in a dose-dependent manner.
It indicated time- and dose-dependent anti-invasive and antimigrative or proliferative and antitoxic effects of hydroalcoholic extract of aerial parts of chamomile on breast cancer cells.
This study demonstrated an effective plant in preventing or treating breast cancer.
Antioxidant compounds in
have anticancer effects.Hydroalcoholic extract of
controls cellular proliferation and apoptosis induction.Hoechst 33342/propidium iodide staining suggested that the extract induces apoptosis more than necrosis.Hydroalcoholic extract of
prevents colonization and cellular migration of human breast cancer MDA-MB-468 and MCF-7 cell lines in a time- and dose-dependent manner.
has low cytotoxic effects on natural cells.
: IARC: International Agency for Research on Cancer; WHO: World Health Organization; FBS: Fetal bovine serum; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; DMSO: Dimethyl sulfoxide; PI: Propidium iodide; LN: Live cells with normal nucleus; LA: Live cells with apoptized nucleus; DN: Dead cells with normal nucleus; DA: Dead cells with apoptized nucleus; BSA: Bovine serum albumin; ANOVA: Analysis of variance; IC
: 50% growth inhibition concentration; GSE: Grape seed extract.</abstract><cop>India</cop><pub>Medknow Publications & Media Pvt Ltd</pub><pmid>28250660</pmid><doi>10.4103/0974-8490.199778</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Original |
| title | The Hydroalcoholic Extract of Matricaria chamomilla Suppresses Migration and Invasion of Human Breast Cancer MDA-MB-468 and MCF-7 Cell Lines |
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