11R-P53 and GM-CSF Expressing Oncolytic Adenovirus Target Cancer Stem Cells with Enhanced Synergistic Activity
Targeting cancer stem cells with oncolytic virus (OV) holds great potential for thorough elimination of cancer cells. Based on our previous studies, we here established 11R-P53 and mGM-CSF carrying oncolytic adenovirus (OAV) SG655-mGMP and investigated its therapeutic effect on hepatocellular carcin...
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| Veröffentlicht in: | Journal of Cancer 2017-01, Vol.8 (2), p.199-206 |
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| container_title | Journal of Cancer |
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| creator | Lv, Sai-Qun Ye, Zhen-Long Liu, Pin-Yi Huang, Yao Li, Lin-Fang Liu, Hui Zhu, Hai-Li Jin, Hua-Jun Qian, Qi-Jun |
| description | Targeting cancer stem cells with oncolytic virus (OV) holds great potential for thorough elimination of cancer cells. Based on our previous studies, we here established 11R-P53 and mGM-CSF carrying oncolytic adenovirus (OAV) SG655-mGMP and investigated its therapeutic effect on hepatocellular carcinoma stem cells Hep3B-C and teratoma stem cells ECCG5. Firstly, the augmenting effect of 11R in our construct was tested and confirmed by examining the expression of EGFP with Fluorescence and FCM assays after transfecting Hep3B-C and ECCG5 cells with OVA SG7605-EGFP and SG7605-11R-EGFP. Secondly, the expressions of 11R-P53 and GM-CSF in Hep3B-C and ECCG5 cells after transfection with OAV SG655-mGMP were detected by Western blot and Elisa assays, respectively. Thirdly, the enhanced growth inhibitory and augmented apoptosis inducing effects of OAV SG655-mGMP on Hep3B-C and ECCG5 cells were tested with FCM assays by comparing with the control, wild type 5 adenovirus, 11R-P53 carrying OVA
. Lastly, the
therapeutic effect of OAV SG655-mGMP toward ECCG5 cell-formed xenografts was studied by measuring tumor volumes post different treatments with PBS, OAV SG655-11R-P53, OAV SG655-mGM-CSF and OAV SG655-mGMP. Treatment with OAV SG655-mGMP induced significant xenograft growth inhibition, inflammation factor AIF1 expression and immune cells infiltration. Therefore, our OAV SG655-mGMP provides a novel platform to arm OVs to target cancer stem cells. |
| doi_str_mv | 10.7150/jca.16406 |
| format | Article |
| fullrecord | <record><control><sourceid>pubmed_cross</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5327369</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>28243324</sourcerecordid><originalsourceid>FETCH-LOGICAL-c375t-b864328da0e50a066791ef1a18ce212f1f79888a1d241bf54e99e14b565c340a3</originalsourceid><addsrcrecordid>eNpVkFtPwjAUxxujEYI8-AVMX30Y9rZuezEhC6AJBiP4vHRdN0pGR9qC7tvLRQmel3OS_-UkPwDuMRpEOERPKykGmDPEr0AXxzQKEs7Z9cXdAX3nVmg_NCERo7egQ2LCKCWsCwzGH8F7SKEwBZy8Bel8DEffG6uc06aCMyObuvVawmGhTLPTduvgQthKeZgKI5WFc6_WMFV17eCX9ks4MsuDUMB5a5SttDumpdc77ds7cFOK2qn-7-6Bz_Fokb4E09nkNR1OA0mj0Ad5zBklcSGQCpFAnEcJViUWOJaKYFLiMkriOBa4IAznZchUkijM8pCHkjIkaA88n3o323ytCqmMt6LONlavhW2zRujsv2L0MquaXRZSElGe7AseTwXSNs5ZVZ6zGGUH7tmee3bkvvc-XD47O_8o0x8M7n4h</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>11R-P53 and GM-CSF Expressing Oncolytic Adenovirus Target Cancer Stem Cells with Enhanced Synergistic Activity</title><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>PubMed Central Open Access</source><creator>Lv, Sai-Qun ; Ye, Zhen-Long ; Liu, Pin-Yi ; Huang, Yao ; Li, Lin-Fang ; Liu, Hui ; Zhu, Hai-Li ; Jin, Hua-Jun ; Qian, Qi-Jun</creator><creatorcontrib>Lv, Sai-Qun ; Ye, Zhen-Long ; Liu, Pin-Yi ; Huang, Yao ; Li, Lin-Fang ; Liu, Hui ; Zhu, Hai-Li ; Jin, Hua-Jun ; Qian, Qi-Jun</creatorcontrib><description>Targeting cancer stem cells with oncolytic virus (OV) holds great potential for thorough elimination of cancer cells. Based on our previous studies, we here established 11R-P53 and mGM-CSF carrying oncolytic adenovirus (OAV) SG655-mGMP and investigated its therapeutic effect on hepatocellular carcinoma stem cells Hep3B-C and teratoma stem cells ECCG5. Firstly, the augmenting effect of 11R in our construct was tested and confirmed by examining the expression of EGFP with Fluorescence and FCM assays after transfecting Hep3B-C and ECCG5 cells with OVA SG7605-EGFP and SG7605-11R-EGFP. Secondly, the expressions of 11R-P53 and GM-CSF in Hep3B-C and ECCG5 cells after transfection with OAV SG655-mGMP were detected by Western blot and Elisa assays, respectively. Thirdly, the enhanced growth inhibitory and augmented apoptosis inducing effects of OAV SG655-mGMP on Hep3B-C and ECCG5 cells were tested with FCM assays by comparing with the control, wild type 5 adenovirus, 11R-P53 carrying OVA
. Lastly, the
therapeutic effect of OAV SG655-mGMP toward ECCG5 cell-formed xenografts was studied by measuring tumor volumes post different treatments with PBS, OAV SG655-11R-P53, OAV SG655-mGM-CSF and OAV SG655-mGMP. Treatment with OAV SG655-mGMP induced significant xenograft growth inhibition, inflammation factor AIF1 expression and immune cells infiltration. Therefore, our OAV SG655-mGMP provides a novel platform to arm OVs to target cancer stem cells.</description><identifier>ISSN: 1837-9664</identifier><identifier>EISSN: 1837-9664</identifier><identifier>DOI: 10.7150/jca.16406</identifier><identifier>PMID: 28243324</identifier><language>eng</language><publisher>Australia: Ivyspring International Publisher</publisher><subject>Research Paper</subject><ispartof>Journal of Cancer, 2017-01, Vol.8 (2), p.199-206</ispartof><rights>Ivyspring International Publisher 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-b864328da0e50a066791ef1a18ce212f1f79888a1d241bf54e99e14b565c340a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5327369/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5327369/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28243324$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lv, Sai-Qun</creatorcontrib><creatorcontrib>Ye, Zhen-Long</creatorcontrib><creatorcontrib>Liu, Pin-Yi</creatorcontrib><creatorcontrib>Huang, Yao</creatorcontrib><creatorcontrib>Li, Lin-Fang</creatorcontrib><creatorcontrib>Liu, Hui</creatorcontrib><creatorcontrib>Zhu, Hai-Li</creatorcontrib><creatorcontrib>Jin, Hua-Jun</creatorcontrib><creatorcontrib>Qian, Qi-Jun</creatorcontrib><title>11R-P53 and GM-CSF Expressing Oncolytic Adenovirus Target Cancer Stem Cells with Enhanced Synergistic Activity</title><title>Journal of Cancer</title><addtitle>J Cancer</addtitle><description>Targeting cancer stem cells with oncolytic virus (OV) holds great potential for thorough elimination of cancer cells. Based on our previous studies, we here established 11R-P53 and mGM-CSF carrying oncolytic adenovirus (OAV) SG655-mGMP and investigated its therapeutic effect on hepatocellular carcinoma stem cells Hep3B-C and teratoma stem cells ECCG5. Firstly, the augmenting effect of 11R in our construct was tested and confirmed by examining the expression of EGFP with Fluorescence and FCM assays after transfecting Hep3B-C and ECCG5 cells with OVA SG7605-EGFP and SG7605-11R-EGFP. Secondly, the expressions of 11R-P53 and GM-CSF in Hep3B-C and ECCG5 cells after transfection with OAV SG655-mGMP were detected by Western blot and Elisa assays, respectively. Thirdly, the enhanced growth inhibitory and augmented apoptosis inducing effects of OAV SG655-mGMP on Hep3B-C and ECCG5 cells were tested with FCM assays by comparing with the control, wild type 5 adenovirus, 11R-P53 carrying OVA
. Lastly, the
therapeutic effect of OAV SG655-mGMP toward ECCG5 cell-formed xenografts was studied by measuring tumor volumes post different treatments with PBS, OAV SG655-11R-P53, OAV SG655-mGM-CSF and OAV SG655-mGMP. Treatment with OAV SG655-mGMP induced significant xenograft growth inhibition, inflammation factor AIF1 expression and immune cells infiltration. Therefore, our OAV SG655-mGMP provides a novel platform to arm OVs to target cancer stem cells.</description><subject>Research Paper</subject><issn>1837-9664</issn><issn>1837-9664</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNpVkFtPwjAUxxujEYI8-AVMX30Y9rZuezEhC6AJBiP4vHRdN0pGR9qC7tvLRQmel3OS_-UkPwDuMRpEOERPKykGmDPEr0AXxzQKEs7Z9cXdAX3nVmg_NCERo7egQ2LCKCWsCwzGH8F7SKEwBZy8Bel8DEffG6uc06aCMyObuvVawmGhTLPTduvgQthKeZgKI5WFc6_WMFV17eCX9ks4MsuDUMB5a5SttDumpdc77ds7cFOK2qn-7-6Bz_Fokb4E09nkNR1OA0mj0Ad5zBklcSGQCpFAnEcJViUWOJaKYFLiMkriOBa4IAznZchUkijM8pCHkjIkaA88n3o323ytCqmMt6LONlavhW2zRujsv2L0MquaXRZSElGe7AseTwXSNs5ZVZ6zGGUH7tmee3bkvvc-XD47O_8o0x8M7n4h</recordid><startdate>20170101</startdate><enddate>20170101</enddate><creator>Lv, Sai-Qun</creator><creator>Ye, Zhen-Long</creator><creator>Liu, Pin-Yi</creator><creator>Huang, Yao</creator><creator>Li, Lin-Fang</creator><creator>Liu, Hui</creator><creator>Zhu, Hai-Li</creator><creator>Jin, Hua-Jun</creator><creator>Qian, Qi-Jun</creator><general>Ivyspring International Publisher</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>20170101</creationdate><title>11R-P53 and GM-CSF Expressing Oncolytic Adenovirus Target Cancer Stem Cells with Enhanced Synergistic Activity</title><author>Lv, Sai-Qun ; Ye, Zhen-Long ; Liu, Pin-Yi ; Huang, Yao ; Li, Lin-Fang ; Liu, Hui ; Zhu, Hai-Li ; Jin, Hua-Jun ; Qian, Qi-Jun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-b864328da0e50a066791ef1a18ce212f1f79888a1d241bf54e99e14b565c340a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Research Paper</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lv, Sai-Qun</creatorcontrib><creatorcontrib>Ye, Zhen-Long</creatorcontrib><creatorcontrib>Liu, Pin-Yi</creatorcontrib><creatorcontrib>Huang, Yao</creatorcontrib><creatorcontrib>Li, Lin-Fang</creatorcontrib><creatorcontrib>Liu, Hui</creatorcontrib><creatorcontrib>Zhu, Hai-Li</creatorcontrib><creatorcontrib>Jin, Hua-Jun</creatorcontrib><creatorcontrib>Qian, Qi-Jun</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lv, Sai-Qun</au><au>Ye, Zhen-Long</au><au>Liu, Pin-Yi</au><au>Huang, Yao</au><au>Li, Lin-Fang</au><au>Liu, Hui</au><au>Zhu, Hai-Li</au><au>Jin, Hua-Jun</au><au>Qian, Qi-Jun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>11R-P53 and GM-CSF Expressing Oncolytic Adenovirus Target Cancer Stem Cells with Enhanced Synergistic Activity</atitle><jtitle>Journal of Cancer</jtitle><addtitle>J Cancer</addtitle><date>2017-01-01</date><risdate>2017</risdate><volume>8</volume><issue>2</issue><spage>199</spage><epage>206</epage><pages>199-206</pages><issn>1837-9664</issn><eissn>1837-9664</eissn><abstract>Targeting cancer stem cells with oncolytic virus (OV) holds great potential for thorough elimination of cancer cells. Based on our previous studies, we here established 11R-P53 and mGM-CSF carrying oncolytic adenovirus (OAV) SG655-mGMP and investigated its therapeutic effect on hepatocellular carcinoma stem cells Hep3B-C and teratoma stem cells ECCG5. Firstly, the augmenting effect of 11R in our construct was tested and confirmed by examining the expression of EGFP with Fluorescence and FCM assays after transfecting Hep3B-C and ECCG5 cells with OVA SG7605-EGFP and SG7605-11R-EGFP. Secondly, the expressions of 11R-P53 and GM-CSF in Hep3B-C and ECCG5 cells after transfection with OAV SG655-mGMP were detected by Western blot and Elisa assays, respectively. Thirdly, the enhanced growth inhibitory and augmented apoptosis inducing effects of OAV SG655-mGMP on Hep3B-C and ECCG5 cells were tested with FCM assays by comparing with the control, wild type 5 adenovirus, 11R-P53 carrying OVA
. Lastly, the
therapeutic effect of OAV SG655-mGMP toward ECCG5 cell-formed xenografts was studied by measuring tumor volumes post different treatments with PBS, OAV SG655-11R-P53, OAV SG655-mGM-CSF and OAV SG655-mGMP. Treatment with OAV SG655-mGMP induced significant xenograft growth inhibition, inflammation factor AIF1 expression and immune cells infiltration. Therefore, our OAV SG655-mGMP provides a novel platform to arm OVs to target cancer stem cells.</abstract><cop>Australia</cop><pub>Ivyspring International Publisher</pub><pmid>28243324</pmid><doi>10.7150/jca.16406</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Research Paper |
| title | 11R-P53 and GM-CSF Expressing Oncolytic Adenovirus Target Cancer Stem Cells with Enhanced Synergistic Activity |
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