Role of multiplex polymerase chain reaction in diagnosing tubercular meningitis
Abstract Tuberculous meningitis (TBM) is one of the most serious manifestations of extrapulmonary tuberculosis. Timely and accurate diagnosis provides a favorable prognosis in patients with TBM. The study evaluated the use of multiplex polymerase chain reaction (PCR) in the diagnosis of TBM. A study...
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| Veröffentlicht in: | Journal of laboratory physicians 2017-04, Vol.9 (2), p.145-147 |
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| creator | Berwal, Anupam Chawla, Kiran Vishwanath, Shashidhar Shenoy, Vishnu Prasad |
| description | Abstract
Tuberculous meningitis (TBM) is one of the most serious manifestations of extrapulmonary tuberculosis. Timely and accurate diagnosis provides a favorable prognosis in patients with TBM. The study evaluated the use of multiplex polymerase chain reaction (PCR) in the diagnosis of TBM. A study was conducted on 74 patients clinically suspected with TBM. The cerebrospinal fluid (CSF) specimens were processed for smear microscopy, middle brook 7H9 culture, and multiplex PCR using primers directed against IS6110 gene and 38 kD protein for detection of Mycobacterium tuberculosis. The results were analyzed to assess the role of multiplex PCR in the diagnosis of TBM. A total of 26 (35.1%) patients were diagnosed with TBM. Microscopy was negative in all while culture was positive in two cases only. Comparing with clinical diagnosis and CSF adenosine deaminase levels of ≥ 10 U/L, multiplex PCR showed sensitivity, specificity, positive predictive value, and negative predictive value of 71.4%, 89.6%, 83.3%, and 81.2%, respectively, in the diagnosis of TBM. |
| doi_str_mv | 10.4103/0974-2727.199633 |
| format | Article |
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Tuberculous meningitis (TBM) is one of the most serious manifestations of extrapulmonary tuberculosis. Timely and accurate diagnosis provides a favorable prognosis in patients with TBM. The study evaluated the use of multiplex polymerase chain reaction (PCR) in the diagnosis of TBM. A study was conducted on 74 patients clinically suspected with TBM. The cerebrospinal fluid (CSF) specimens were processed for smear microscopy, middle brook 7H9 culture, and multiplex PCR using primers directed against IS6110 gene and 38 kD protein for detection of Mycobacterium tuberculosis. The results were analyzed to assess the role of multiplex PCR in the diagnosis of TBM. A total of 26 (35.1%) patients were diagnosed with TBM. Microscopy was negative in all while culture was positive in two cases only. Comparing with clinical diagnosis and CSF adenosine deaminase levels of ≥ 10 U/L, multiplex PCR showed sensitivity, specificity, positive predictive value, and negative predictive value of 71.4%, 89.6%, 83.3%, and 81.2%, respectively, in the diagnosis of TBM.</description><identifier>ISSN: 0974-2727</identifier><identifier>EISSN: 0974-7826</identifier><identifier>DOI: 10.4103/0974-2727.199633</identifier><identifier>PMID: 28367034</identifier><language>eng</language><publisher>A-12, Second Floor, Sector -2, NOIDA -201301, India: Thieme Medical and Scientific Publishers Private Ltd</publisher><subject>Bacterial meningitis ; Brief Report ; Diagnosis ; Genetic aspects ; Innovations ; Molecular diagnostic techniques ; Polymerase chain reaction</subject><ispartof>Journal of laboratory physicians, 2017-04, Vol.9 (2), p.145-147</ispartof><rights>COPYRIGHT 2017 Medknow Publications and Media Pvt. Ltd.</rights><rights>Copyright Medknow Publications & Media Pvt Ltd Apr-Jun 2017</rights><rights>Copyright: © 2017 Journal of Laboratory Physicians 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c404e-e25d358712fbe135df492f66a5102c6096c7252e21a20e8f00d829722d0edd103</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5320881/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5320881/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,20870,27901,27902,53766,53768,54562</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28367034$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Berwal, Anupam</creatorcontrib><creatorcontrib>Chawla, Kiran</creatorcontrib><creatorcontrib>Vishwanath, Shashidhar</creatorcontrib><creatorcontrib>Shenoy, Vishnu Prasad</creatorcontrib><title>Role of multiplex polymerase chain reaction in diagnosing tubercular meningitis</title><title>Journal of laboratory physicians</title><addtitle>J Lab Physicians</addtitle><description>Abstract
Tuberculous meningitis (TBM) is one of the most serious manifestations of extrapulmonary tuberculosis. Timely and accurate diagnosis provides a favorable prognosis in patients with TBM. The study evaluated the use of multiplex polymerase chain reaction (PCR) in the diagnosis of TBM. A study was conducted on 74 patients clinically suspected with TBM. The cerebrospinal fluid (CSF) specimens were processed for smear microscopy, middle brook 7H9 culture, and multiplex PCR using primers directed against IS6110 gene and 38 kD protein for detection of Mycobacterium tuberculosis. The results were analyzed to assess the role of multiplex PCR in the diagnosis of TBM. A total of 26 (35.1%) patients were diagnosed with TBM. Microscopy was negative in all while culture was positive in two cases only. Comparing with clinical diagnosis and CSF adenosine deaminase levels of ≥ 10 U/L, multiplex PCR showed sensitivity, specificity, positive predictive value, and negative predictive value of 71.4%, 89.6%, 83.3%, and 81.2%, respectively, in the diagnosis of TBM.</description><subject>Bacterial meningitis</subject><subject>Brief Report</subject><subject>Diagnosis</subject><subject>Genetic aspects</subject><subject>Innovations</subject><subject>Molecular diagnostic techniques</subject><subject>Polymerase chain reaction</subject><issn>0974-2727</issn><issn>0974-7826</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>0U6</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp1kc1r3DAQxU1paUKae0_F0EsvTvRhydKlEEI_CQRKexZaebSrRJa2kp1t_vvK9SbdFKqLxMxvnubxquo1RmctRvQcya5tSEe6Mywlp_RZdfyn1AnCn-_fc_uoOs35BpVDZYta8bI6IoLyDtH2uLr-Fj3U0dbD5Ee39fCr3kZ_P0DSGWqz0S7UCbQZXQx1efdOr0PMLqzrcVpBMpPXqR4glIobXX5VvbDaZzjd3yfVj48fvl9-bq6uP325vLhqTFkBGiCsp0x0mNgVYMp620piOdcMI2I4ktx0hBEgWBMEwiLUCyI7QnoEfV_cn1TvF93ttBqgNxDGpL3aJjfodK-iduppJ7iNWsc7xShBQuAi8G4vkOLPCfKoBpcNeK8DxCkrLAQVLZKIFvTtP-hNnFIo9grFOZcSY_aXWmsPygUby79mFlUXrUCMY0lFodBCmRRzTmAfV8ZIzbGqOTc156aWWMvIm0OrjwMPIRbg6wLsoh8h5Vs_7SCpwt6GuHsi3BwIK9wyNcevolUP8Rex80Vs3DgY4MDq__b7DVFiwvM</recordid><startdate>201704</startdate><enddate>201704</enddate><creator>Berwal, Anupam</creator><creator>Chawla, Kiran</creator><creator>Vishwanath, Shashidhar</creator><creator>Shenoy, Vishnu Prasad</creator><general>Thieme Medical and Scientific Publishers Private Ltd</general><general>Wolters Kluwer India Pvt. 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Tuberculous meningitis (TBM) is one of the most serious manifestations of extrapulmonary tuberculosis. Timely and accurate diagnosis provides a favorable prognosis in patients with TBM. The study evaluated the use of multiplex polymerase chain reaction (PCR) in the diagnosis of TBM. A study was conducted on 74 patients clinically suspected with TBM. The cerebrospinal fluid (CSF) specimens were processed for smear microscopy, middle brook 7H9 culture, and multiplex PCR using primers directed against IS6110 gene and 38 kD protein for detection of Mycobacterium tuberculosis. The results were analyzed to assess the role of multiplex PCR in the diagnosis of TBM. A total of 26 (35.1%) patients were diagnosed with TBM. Microscopy was negative in all while culture was positive in two cases only. Comparing with clinical diagnosis and CSF adenosine deaminase levels of ≥ 10 U/L, multiplex PCR showed sensitivity, specificity, positive predictive value, and negative predictive value of 71.4%, 89.6%, 83.3%, and 81.2%, respectively, in the diagnosis of TBM.</abstract><cop>A-12, Second Floor, Sector -2, NOIDA -201301, India</cop><pub>Thieme Medical and Scientific Publishers Private Ltd</pub><pmid>28367034</pmid><doi>10.4103/0974-2727.199633</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection; PubMed Central Open Access; Thieme Connect Journals Open Access |
| subjects | Bacterial meningitis Brief Report Diagnosis Genetic aspects Innovations Molecular diagnostic techniques Polymerase chain reaction |
| title | Role of multiplex polymerase chain reaction in diagnosing tubercular meningitis |
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