P450BM3 fused to phosphite dehydrogenase allows phosphite-driven selective oxidations

To facilitate the wider application of the NADPH-dependent P450 BM3, we fused the monooxygenase with a phosphite dehydrogenase (PTDH). The resulting monooxygenase-dehydrogenase fusion enzyme acts as a self-sufficient bifunctional catalyst, accepting phosphite as a cheap electron donor for the regene...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Applied microbiology and biotechnology 2017-03, Vol.101 (6), p.2319-2331
Hauptverfasser:	Beyer, Nina, Kulig, Justyna K., Bartsch, Anette, Hayes, Martin A., Janssen, Dick B., Fraaije, Marco W.
Format:	Artikel
Sprache:	eng
Schlagworte:	biocatalysts Biomedical and Life Sciences Biotechnologically Relevant Enzymes and Proteins Biotechnology dodecanoic acid drugs enzymes hydroxylation Life Sciences Microbial Genetics and Genomics Microbiology NADP (coenzyme) pharmacokinetics
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!