Geosmin-producing Species of Coelosphaerium (Synechococcales, Cyanobacteria) in Lake Shinji, Japan
In Lake Shinji, Japan, periodic outbreaks of musty odour have occurred since mid-May 2007. Although the substance responsible for the odour was identified as geosmin, the odour-producing organism was unknown. We cultivated an axenic unialgal strain and determined that a species of Coelosphaerium (Sy...
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| Veröffentlicht in: | Scientific reports 2017-02, Vol.7 (1), p.41928-41928, Article 41928 |
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| creator | Godo, T. Saki, Y. Nojiri, Y. Tsujitani, M. Sugahara, S. Hayashi, S. Kamiya, H. Ohtani, S. Seike, Y. |
| description | In Lake Shinji, Japan, periodic outbreaks of musty odour have occurred since mid-May 2007. Although the substance responsible for the odour was identified as geosmin, the odour-producing organism was unknown. We cultivated an axenic unialgal strain and determined that a species of
Coelosphaerium
(Synechococcales) was responsible for the production of geosmin in Lake Shinji. Our analysis was conducted using gas chromatography/mass spectrometry to determine the odorous compound. To determine the algae species, it was observed by optical microscopy to describe its morphological characteristics and the polymerase chain reaction was used to characterise the nucleotide sequence of the 16S rRNA gene and the 16S-23S rRNA internal transcribed spacer region. In addition, we explored the relationship between the number of cells of the
Coelosphaerium
sp. and the concentration of geosmin. In conclusion, geosmin, the cause of the musty odour in Lake Shinji in autumn 2009, was produced by
Coelosphaerium
sp., and to our knowledge, this is the first report of a geosmin-producing species in the family Coelosphaeriaceae. |
| doi_str_mv | 10.1038/srep41928 |
| format | Article |
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Coelosphaerium
(Synechococcales) was responsible for the production of geosmin in Lake Shinji. Our analysis was conducted using gas chromatography/mass spectrometry to determine the odorous compound. To determine the algae species, it was observed by optical microscopy to describe its morphological characteristics and the polymerase chain reaction was used to characterise the nucleotide sequence of the 16S rRNA gene and the 16S-23S rRNA internal transcribed spacer region. In addition, we explored the relationship between the number of cells of the
Coelosphaerium
sp. and the concentration of geosmin. In conclusion, geosmin, the cause of the musty odour in Lake Shinji in autumn 2009, was produced by
Coelosphaerium
sp., and to our knowledge, this is the first report of a geosmin-producing species in the family Coelosphaeriaceae.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep41928</identifier><identifier>PMID: 28195147</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/45 ; 704/286 ; Algae ; Chromatography ; Cyanobacteria ; Cyanobacteria - cytology ; Cyanobacteria - genetics ; Cyanobacteria - isolation & purification ; Cyanobacteria - metabolism ; Drinking water ; Environmental science ; Fisheries ; Gas chromatography ; Geosmin ; Humanities and Social Sciences ; Lakes ; Lakes - microbiology ; Light microscopy ; Mass spectrometry ; Mass spectroscopy ; Morphology ; multidisciplinary ; Naphthols - metabolism ; Nucleotide sequence ; Odor ; Odors ; Organisms ; Outbreaks ; Physical characteristics ; Plankton ; Polymerase chain reaction ; Public health ; RNA, Ribosomal, 16S - genetics ; RNA, Ribosomal, 23S - genetics ; rRNA 16S ; rRNA 23S ; Science ; Scientific imaging ; Spacer region ; Species ; Water analysis</subject><ispartof>Scientific reports, 2017-02, Vol.7 (1), p.41928-41928, Article 41928</ispartof><rights>The Author(s) 2017</rights><rights>Copyright Nature Publishing Group Feb 2017</rights><rights>Copyright © 2017, The Author(s) 2017 The Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c548t-209f438fe946f4593ddec01f9d4ff23ee2b3c73c2e7b54bb5d42dbe1d1e7ba603</citedby><cites>FETCH-LOGICAL-c548t-209f438fe946f4593ddec01f9d4ff23ee2b3c73c2e7b54bb5d42dbe1d1e7ba603</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5307322/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5307322/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,27905,27906,41101,42170,51557,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28195147$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Godo, T.</creatorcontrib><creatorcontrib>Saki, Y.</creatorcontrib><creatorcontrib>Nojiri, Y.</creatorcontrib><creatorcontrib>Tsujitani, M.</creatorcontrib><creatorcontrib>Sugahara, S.</creatorcontrib><creatorcontrib>Hayashi, S.</creatorcontrib><creatorcontrib>Kamiya, H.</creatorcontrib><creatorcontrib>Ohtani, S.</creatorcontrib><creatorcontrib>Seike, Y.</creatorcontrib><title>Geosmin-producing Species of Coelosphaerium (Synechococcales, Cyanobacteria) in Lake Shinji, Japan</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>In Lake Shinji, Japan, periodic outbreaks of musty odour have occurred since mid-May 2007. Although the substance responsible for the odour was identified as geosmin, the odour-producing organism was unknown. We cultivated an axenic unialgal strain and determined that a species of
Coelosphaerium
(Synechococcales) was responsible for the production of geosmin in Lake Shinji. Our analysis was conducted using gas chromatography/mass spectrometry to determine the odorous compound. To determine the algae species, it was observed by optical microscopy to describe its morphological characteristics and the polymerase chain reaction was used to characterise the nucleotide sequence of the 16S rRNA gene and the 16S-23S rRNA internal transcribed spacer region. In addition, we explored the relationship between the number of cells of the
Coelosphaerium
sp. and the concentration of geosmin. In conclusion, geosmin, the cause of the musty odour in Lake Shinji in autumn 2009, was produced by
Coelosphaerium
sp., and to our knowledge, this is the first report of a geosmin-producing species in the family Coelosphaeriaceae.</description><subject>631/45</subject><subject>704/286</subject><subject>Algae</subject><subject>Chromatography</subject><subject>Cyanobacteria</subject><subject>Cyanobacteria - cytology</subject><subject>Cyanobacteria - genetics</subject><subject>Cyanobacteria - isolation & purification</subject><subject>Cyanobacteria - metabolism</subject><subject>Drinking water</subject><subject>Environmental science</subject><subject>Fisheries</subject><subject>Gas chromatography</subject><subject>Geosmin</subject><subject>Humanities and Social Sciences</subject><subject>Lakes</subject><subject>Lakes - microbiology</subject><subject>Light microscopy</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Morphology</subject><subject>multidisciplinary</subject><subject>Naphthols - metabolism</subject><subject>Nucleotide sequence</subject><subject>Odor</subject><subject>Odors</subject><subject>Organisms</subject><subject>Outbreaks</subject><subject>Physical characteristics</subject><subject>Plankton</subject><subject>Polymerase chain reaction</subject><subject>Public health</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>RNA, Ribosomal, 23S - genetics</subject><subject>rRNA 16S</subject><subject>rRNA 23S</subject><subject>Science</subject><subject>Scientific imaging</subject><subject>Spacer region</subject><subject>Species</subject><subject>Water analysis</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNplkU9rGzEQxUVpaEKSQ79AEfSSlGyrv97VpVBMk6YYenByFlrtyJa7K20lb8HfvgpOjNvqMkLz483TPITeUvKREt58yglGQRVrXqEzRoSsGGfs9dH9FF3mvCHlSKYK-QadsoYqSUV9hto7iHnwoRpT7CbrwwovR7AeMo4OzyP0MY9rA8lPA75a7gLYdbTRWtNDvsHznQmxNXZbAHONfcAL8xPwcu3Dxt_g72Y04QKdONNnuHyu5-jx9uvD_Fu1-HF3P_-yqKwUzbZiRDnBGwdKzJyQincdWEKd6oRzjAOwltuaWwZ1K0Xbyk6wrgXa0fJgZoSfo8973XFqB-gshG0yvR6TH0za6Wi8_rsT_Fqv4m8tOanLnorA1bNAir8myFs9-Gyh702AOGVNm1nDVc0kL-j7f9BNnFIo39NUEVoz0nBRqOs9ZVPMJSd3MEOJfgpPH8Ir7Ltj9wfyJaoCfNgDubTCCtLRyP_U_gBxKqSl</recordid><startdate>20170214</startdate><enddate>20170214</enddate><creator>Godo, T.</creator><creator>Saki, Y.</creator><creator>Nojiri, Y.</creator><creator>Tsujitani, M.</creator><creator>Sugahara, S.</creator><creator>Hayashi, S.</creator><creator>Kamiya, H.</creator><creator>Ohtani, S.</creator><creator>Seike, Y.</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20170214</creationdate><title>Geosmin-producing Species of Coelosphaerium (Synechococcales, Cyanobacteria) in Lake Shinji, Japan</title><author>Godo, T. ; Saki, Y. ; Nojiri, Y. ; Tsujitani, M. ; Sugahara, S. ; Hayashi, S. ; Kamiya, H. ; Ohtani, S. ; Seike, Y.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c548t-209f438fe946f4593ddec01f9d4ff23ee2b3c73c2e7b54bb5d42dbe1d1e7ba603</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>631/45</topic><topic>704/286</topic><topic>Algae</topic><topic>Chromatography</topic><topic>Cyanobacteria</topic><topic>Cyanobacteria - cytology</topic><topic>Cyanobacteria - genetics</topic><topic>Cyanobacteria - isolation & purification</topic><topic>Cyanobacteria - metabolism</topic><topic>Drinking water</topic><topic>Environmental science</topic><topic>Fisheries</topic><topic>Gas chromatography</topic><topic>Geosmin</topic><topic>Humanities and Social Sciences</topic><topic>Lakes</topic><topic>Lakes - microbiology</topic><topic>Light microscopy</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Morphology</topic><topic>multidisciplinary</topic><topic>Naphthols - metabolism</topic><topic>Nucleotide sequence</topic><topic>Odor</topic><topic>Odors</topic><topic>Organisms</topic><topic>Outbreaks</topic><topic>Physical characteristics</topic><topic>Plankton</topic><topic>Polymerase chain reaction</topic><topic>Public health</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>RNA, Ribosomal, 23S - genetics</topic><topic>rRNA 16S</topic><topic>rRNA 23S</topic><topic>Science</topic><topic>Scientific imaging</topic><topic>Spacer region</topic><topic>Species</topic><topic>Water analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Godo, T.</creatorcontrib><creatorcontrib>Saki, Y.</creatorcontrib><creatorcontrib>Nojiri, Y.</creatorcontrib><creatorcontrib>Tsujitani, M.</creatorcontrib><creatorcontrib>Sugahara, S.</creatorcontrib><creatorcontrib>Hayashi, S.</creatorcontrib><creatorcontrib>Kamiya, H.</creatorcontrib><creatorcontrib>Ohtani, S.</creatorcontrib><creatorcontrib>Seike, Y.</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Godo, T.</au><au>Saki, Y.</au><au>Nojiri, Y.</au><au>Tsujitani, M.</au><au>Sugahara, S.</au><au>Hayashi, S.</au><au>Kamiya, H.</au><au>Ohtani, S.</au><au>Seike, Y.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Geosmin-producing Species of Coelosphaerium (Synechococcales, Cyanobacteria) in Lake Shinji, Japan</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2017-02-14</date><risdate>2017</risdate><volume>7</volume><issue>1</issue><spage>41928</spage><epage>41928</epage><pages>41928-41928</pages><artnum>41928</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>In Lake Shinji, Japan, periodic outbreaks of musty odour have occurred since mid-May 2007. Although the substance responsible for the odour was identified as geosmin, the odour-producing organism was unknown. We cultivated an axenic unialgal strain and determined that a species of
Coelosphaerium
(Synechococcales) was responsible for the production of geosmin in Lake Shinji. Our analysis was conducted using gas chromatography/mass spectrometry to determine the odorous compound. To determine the algae species, it was observed by optical microscopy to describe its morphological characteristics and the polymerase chain reaction was used to characterise the nucleotide sequence of the 16S rRNA gene and the 16S-23S rRNA internal transcribed spacer region. In addition, we explored the relationship between the number of cells of the
Coelosphaerium
sp. and the concentration of geosmin. In conclusion, geosmin, the cause of the musty odour in Lake Shinji in autumn 2009, was produced by
Coelosphaerium
sp., and to our knowledge, this is the first report of a geosmin-producing species in the family Coelosphaeriaceae.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>28195147</pmid><doi>10.1038/srep41928</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 631/45 704/286 Algae Chromatography Cyanobacteria Cyanobacteria - cytology Cyanobacteria - genetics Cyanobacteria - isolation & purification Cyanobacteria - metabolism Drinking water Environmental science Fisheries Gas chromatography Geosmin Humanities and Social Sciences Lakes Lakes - microbiology Light microscopy Mass spectrometry Mass spectroscopy Morphology multidisciplinary Naphthols - metabolism Nucleotide sequence Odor Odors Organisms Outbreaks Physical characteristics Plankton Polymerase chain reaction Public health RNA, Ribosomal, 16S - genetics RNA, Ribosomal, 23S - genetics rRNA 16S rRNA 23S Science Scientific imaging Spacer region Species Water analysis |
| title | Geosmin-producing Species of Coelosphaerium (Synechococcales, Cyanobacteria) in Lake Shinji, Japan |
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