Characterization and robust nature of newly isolated oleaginous marine yeast Rhodosporidium spp. from coastal water of Northern China
A total of ten marine yeast strains isolated from Bohai Sea, Northern China were identified to be members of three genera Rhodosporidium , Rhodotorula , and Cryptococcus . Two representative strains Rhodosporidium TJUWZ4 and Cryptococcus TJUWZA11 with high lipid content based on Nile red staining me...
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| creator | Wang, Qiuzhen Cui, Yan Sen, Biswarup Ma, Wenmeng Zheng, Rose Lynn Liu, Xianhua Wang, Guangyi |
| description | A total of ten marine yeast strains isolated from Bohai Sea, Northern China were identified to be members of three genera
Rhodosporidium
,
Rhodotorula
, and
Cryptococcus
. Two representative strains
Rhodosporidium
TJUWZ4 and
Cryptococcus
TJUWZA11 with high lipid content based on Nile red staining method were further characterized. A wide range of culture conditions (C and N sources, pH, temperature, salinity and C/N ratio) were tested to characterize the biomass and lipid production (yield and productivity) of these strains. Results indicated that
Rhodosporidium
TJUWZ4 was capable of achieving lipid yield up to 44% and 0.09 g/l–h productivity on glucose and peptone medium at pH 4, 20 °C, 30% salinity, and C/N 80. Three fatty acids, namely oleic acid (18:1), palmitic acid (C16:0) and linoleic acid (18:2) were the major intracellular fatty acids, which accounted for 90% of total lipids. With promising features for intracellular lipid accumulation,
Rhodosporidium
TJUWZ4 is a robust strain with great potentials for application in biodiesel production from renewable feedstocks. |
| doi_str_mv | 10.1186/s13568-017-0329-x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5302000</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1863693961</sourcerecordid><originalsourceid>FETCH-LOGICAL-c503t-bb115956545023451d943c01b861e25923fc7d97bc2e9707e2c9b01c9aab28093</originalsourceid><addsrcrecordid>eNqNkkGL1TAUhYsozjDOD3AjATduOuYmTZtsBHnoKAwKouuQprevGdqkJq0zz_38b_N44_AUBLNJ4H7nJPfmFMVzoBcAsn6dgItalhSaknKmyttHxSkDBSWVQjw-Op8U5yld07wEpaoWT4sTJqGqpJSnxd1mMNHYBaP7aRYXPDG-IzG0a1qIN8sakYSeeLwZd8SlMJoFOxJGNFvnw5rIZKLzSHZosuDLELqQ5hBd59aJpHm-IH0ME7Ehl81IbrI87g0_hbgMGD3ZDM6bZ8WT3owJz-_3s-Lb-3dfNx_Kq8-XHzdvr0orKF_KtgUQStSiEpTxSkCnKm4ptLIGZEIx3tumU01rGaqGNsisailYZUzLJFX8rHhz8J3XdsLOol-iGfUcXW5jp4Nx-s-Kd4Pehh9acMryALPBq3uDGL6vmBY9uWRxHI3HPA2dP0ZyLgD4_6C8VlzVkNGXf6HXYY0-T2JPNYqzum4yBQfKxpBSxP7h3UD1PhL6EAmdI6H3kdC3WfPiuOEHxe8AZIAdgJRLfovx6Op_uv4CqefD2A</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1867932667</pqid></control><display><type>article</type><title>Characterization and robust nature of newly isolated oleaginous marine yeast Rhodosporidium spp. from coastal water of Northern China</title><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central Open Access</source><source>PubMed Central</source><source>Springer Nature OA/Free Journals</source><source>Free Full-Text Journals in Chemistry</source><source>SpringerLink Journals - AutoHoldings</source><creator>Wang, Qiuzhen ; Cui, Yan ; Sen, Biswarup ; Ma, Wenmeng ; Zheng, Rose Lynn ; Liu, Xianhua ; Wang, Guangyi</creator><creatorcontrib>Wang, Qiuzhen ; Cui, Yan ; Sen, Biswarup ; Ma, Wenmeng ; Zheng, Rose Lynn ; Liu, Xianhua ; Wang, Guangyi</creatorcontrib><description>A total of ten marine yeast strains isolated from Bohai Sea, Northern China were identified to be members of three genera
Rhodosporidium
,
Rhodotorula
, and
Cryptococcus
. Two representative strains
Rhodosporidium
TJUWZ4 and
Cryptococcus
TJUWZA11 with high lipid content based on Nile red staining method were further characterized. A wide range of culture conditions (C and N sources, pH, temperature, salinity and C/N ratio) were tested to characterize the biomass and lipid production (yield and productivity) of these strains. Results indicated that
Rhodosporidium
TJUWZ4 was capable of achieving lipid yield up to 44% and 0.09 g/l–h productivity on glucose and peptone medium at pH 4, 20 °C, 30% salinity, and C/N 80. Three fatty acids, namely oleic acid (18:1), palmitic acid (C16:0) and linoleic acid (18:2) were the major intracellular fatty acids, which accounted for 90% of total lipids. With promising features for intracellular lipid accumulation,
Rhodosporidium
TJUWZ4 is a robust strain with great potentials for application in biodiesel production from renewable feedstocks.</description><identifier>ISSN: 2191-0855</identifier><identifier>EISSN: 2191-0855</identifier><identifier>DOI: 10.1186/s13568-017-0329-x</identifier><identifier>PMID: 28144888</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Biomedical and Life Sciences ; Biotechnology ; Cryptococcus ; Life Sciences ; Microbial Genetics and Genomics ; Microbiology ; Original ; Original Article ; Rhodosporidium ; Rhodotorula</subject><ispartof>AMB Express, 2017-02, Vol.7 (1), p.30-30, Article 30</ispartof><rights>The Author(s) 2017</rights><rights>AMB Express is a copyright of Springer, 2017.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c503t-bb115956545023451d943c01b861e25923fc7d97bc2e9707e2c9b01c9aab28093</citedby><cites>FETCH-LOGICAL-c503t-bb115956545023451d943c01b861e25923fc7d97bc2e9707e2c9b01c9aab28093</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5302000/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5302000/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27923,27924,41119,41487,42188,42556,51318,51575,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28144888$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Qiuzhen</creatorcontrib><creatorcontrib>Cui, Yan</creatorcontrib><creatorcontrib>Sen, Biswarup</creatorcontrib><creatorcontrib>Ma, Wenmeng</creatorcontrib><creatorcontrib>Zheng, Rose Lynn</creatorcontrib><creatorcontrib>Liu, Xianhua</creatorcontrib><creatorcontrib>Wang, Guangyi</creatorcontrib><title>Characterization and robust nature of newly isolated oleaginous marine yeast Rhodosporidium spp. from coastal water of Northern China</title><title>AMB Express</title><addtitle>AMB Expr</addtitle><addtitle>AMB Express</addtitle><description>A total of ten marine yeast strains isolated from Bohai Sea, Northern China were identified to be members of three genera
Rhodosporidium
,
Rhodotorula
, and
Cryptococcus
. Two representative strains
Rhodosporidium
TJUWZ4 and
Cryptococcus
TJUWZA11 with high lipid content based on Nile red staining method were further characterized. A wide range of culture conditions (C and N sources, pH, temperature, salinity and C/N ratio) were tested to characterize the biomass and lipid production (yield and productivity) of these strains. Results indicated that
Rhodosporidium
TJUWZ4 was capable of achieving lipid yield up to 44% and 0.09 g/l–h productivity on glucose and peptone medium at pH 4, 20 °C, 30% salinity, and C/N 80. Three fatty acids, namely oleic acid (18:1), palmitic acid (C16:0) and linoleic acid (18:2) were the major intracellular fatty acids, which accounted for 90% of total lipids. With promising features for intracellular lipid accumulation,
Rhodosporidium
TJUWZ4 is a robust strain with great potentials for application in biodiesel production from renewable feedstocks.</description><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Cryptococcus</subject><subject>Life Sciences</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiology</subject><subject>Original</subject><subject>Original Article</subject><subject>Rhodosporidium</subject><subject>Rhodotorula</subject><issn>2191-0855</issn><issn>2191-0855</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkkGL1TAUhYsozjDOD3AjATduOuYmTZtsBHnoKAwKouuQprevGdqkJq0zz_38b_N44_AUBLNJ4H7nJPfmFMVzoBcAsn6dgItalhSaknKmyttHxSkDBSWVQjw-Op8U5yld07wEpaoWT4sTJqGqpJSnxd1mMNHYBaP7aRYXPDG-IzG0a1qIN8sakYSeeLwZd8SlMJoFOxJGNFvnw5rIZKLzSHZosuDLELqQ5hBd59aJpHm-IH0ME7Ehl81IbrI87g0_hbgMGD3ZDM6bZ8WT3owJz-_3s-Lb-3dfNx_Kq8-XHzdvr0orKF_KtgUQStSiEpTxSkCnKm4ptLIGZEIx3tumU01rGaqGNsisailYZUzLJFX8rHhz8J3XdsLOol-iGfUcXW5jp4Nx-s-Kd4Pehh9acMryALPBq3uDGL6vmBY9uWRxHI3HPA2dP0ZyLgD4_6C8VlzVkNGXf6HXYY0-T2JPNYqzum4yBQfKxpBSxP7h3UD1PhL6EAmdI6H3kdC3WfPiuOEHxe8AZIAdgJRLfovx6Op_uv4CqefD2A</recordid><startdate>20170201</startdate><enddate>20170201</enddate><creator>Wang, Qiuzhen</creator><creator>Cui, Yan</creator><creator>Sen, Biswarup</creator><creator>Ma, Wenmeng</creator><creator>Zheng, Rose Lynn</creator><creator>Liu, Xianhua</creator><creator>Wang, Guangyi</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>L6V</scope><scope>LK8</scope><scope>M7P</scope><scope>M7S</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>7X8</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>5PM</scope></search><sort><creationdate>20170201</creationdate><title>Characterization and robust nature of newly isolated oleaginous marine yeast Rhodosporidium spp. from coastal water of Northern China</title><author>Wang, Qiuzhen ; Cui, Yan ; Sen, Biswarup ; Ma, Wenmeng ; Zheng, Rose Lynn ; Liu, Xianhua ; Wang, Guangyi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c503t-bb115956545023451d943c01b861e25923fc7d97bc2e9707e2c9b01c9aab28093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Cryptococcus</topic><topic>Life Sciences</topic><topic>Microbial Genetics and Genomics</topic><topic>Microbiology</topic><topic>Original</topic><topic>Original Article</topic><topic>Rhodosporidium</topic><topic>Rhodotorula</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Qiuzhen</creatorcontrib><creatorcontrib>Cui, Yan</creatorcontrib><creatorcontrib>Sen, Biswarup</creatorcontrib><creatorcontrib>Ma, Wenmeng</creatorcontrib><creatorcontrib>Zheng, Rose Lynn</creatorcontrib><creatorcontrib>Liu, Xianhua</creatorcontrib><creatorcontrib>Wang, Guangyi</creatorcontrib><collection>Springer Nature OA/Free Journals</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>MEDLINE - Academic</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>AMB Express</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Qiuzhen</au><au>Cui, Yan</au><au>Sen, Biswarup</au><au>Ma, Wenmeng</au><au>Zheng, Rose Lynn</au><au>Liu, Xianhua</au><au>Wang, Guangyi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization and robust nature of newly isolated oleaginous marine yeast Rhodosporidium spp. from coastal water of Northern China</atitle><jtitle>AMB Express</jtitle><stitle>AMB Expr</stitle><addtitle>AMB Express</addtitle><date>2017-02-01</date><risdate>2017</risdate><volume>7</volume><issue>1</issue><spage>30</spage><epage>30</epage><pages>30-30</pages><artnum>30</artnum><issn>2191-0855</issn><eissn>2191-0855</eissn><abstract>A total of ten marine yeast strains isolated from Bohai Sea, Northern China were identified to be members of three genera
Rhodosporidium
,
Rhodotorula
, and
Cryptococcus
. Two representative strains
Rhodosporidium
TJUWZ4 and
Cryptococcus
TJUWZA11 with high lipid content based on Nile red staining method were further characterized. A wide range of culture conditions (C and N sources, pH, temperature, salinity and C/N ratio) were tested to characterize the biomass and lipid production (yield and productivity) of these strains. Results indicated that
Rhodosporidium
TJUWZ4 was capable of achieving lipid yield up to 44% and 0.09 g/l–h productivity on glucose and peptone medium at pH 4, 20 °C, 30% salinity, and C/N 80. Three fatty acids, namely oleic acid (18:1), palmitic acid (C16:0) and linoleic acid (18:2) were the major intracellular fatty acids, which accounted for 90% of total lipids. With promising features for intracellular lipid accumulation,
Rhodosporidium
TJUWZ4 is a robust strain with great potentials for application in biodiesel production from renewable feedstocks.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>28144888</pmid><doi>10.1186/s13568-017-0329-x</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Biomedical and Life Sciences Biotechnology Cryptococcus Life Sciences Microbial Genetics and Genomics Microbiology Original Original Article Rhodosporidium Rhodotorula |
| title | Characterization and robust nature of newly isolated oleaginous marine yeast Rhodosporidium spp. from coastal water of Northern China |
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