RNAi-mediated knockdown of mouse melanocortin-4 receptor in vitro and in vivo, using an siRNA expression construct based on the mir-187 precursor

RNA interference (RNAi) is a powerful tool for the study of gene function in mammalian systems, including transgenic mice. Here, we report a gene knockdown system based on the human mir-187 precursor. We introduced small interfering RNA (siRNA) sequences against the mouse melanocortin-4 receptor (mM...

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Veröffentlicht in:	Experimental Animals 2017, Vol.66(1), pp.41-50
Hauptverfasser:	Kato, Minoru, Huang, Yi-Ying, Matsuo, Mina, Takashina, Yoko, Sasaki, Kazuyo, Horai, Yasushi, Juni, Aya, Kamijo, Shin-Ichi, Saigo, Kaoru, Ui-Tei, Kumiko, Tei, Hajime
Format:	Artikel
Sprache:	eng
Schlagworte:	Actins - genetics Animals Chickens - genetics Cytomegalovirus - genetics Gene Knockdown Techniques Genetic Vectors - genetics Herpesviridae Male Mice Mice, Inbred C57BL Mice, Transgenic MicroRNAs - genetics mir-187 mMC4R Original Promoter Regions, Genetic Receptor, Melanocortin, Type 4 - genetics Receptor, Melanocortin, Type 4 - metabolism RNA Interference RNA Polymerase II - genetics RNA, Small Interfering - genetics RNAi Transgenic mice
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