Knockdown of a cellulose synthase gene BoiCesA affects the leaf anatomy, cellulose content and salt tolerance in broccoli
Cellulose is the major component of cell wall materials. A 300 bp specific fragment from the cDNA fragment was chosen to insert into vector pFGC1008 at forward and reverse orientations to construct the recombinant RNAi vector. Knockdown of BoiCesA caused “dwarf” phenotype with smaller leaves and a l...
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| Veröffentlicht in: | Scientific reports 2017-02, Vol.7 (1), p.41397-41397, Article 41397 |
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| creator | Li, Shuangtao Zhang, Lei Wang, Ying Xu, Fengfeng Liu, Mengyun Lin, Peng Ren, Shuxin Ma, Rui Guo, Yang-Dong |
| description | Cellulose is the major component of cell wall materials. A 300 bp specific fragment from the cDNA fragment was chosen to insert into vector pFGC1008 at forward and reverse orientations to construct the recombinant RNAi vector. Knockdown of
BoiCesA
caused “dwarf” phenotype with smaller leaves and a loss of the content of cellulose. Moreover, RT-PCR analysis confirmed that the expression of the RNAi apparatus could repress expression of the
CesA
gene. Meanwhile, examination of the leaves from the T3 of RNAi transformants indicated reduction of cell expansion in vascular bundles, particularly on their abaxial surface. The proline and soluble sugar content increased contrarily. Under the salt stress, the T3 of RNAi plants showed significant higher resistance. The expression levels of some salt tolerance related genes (
BoiProH, BoiPIP2;2, BoiPIP2;3
) were significantly changed in T3 of RNAi plants. The results showed that the hairpin structure of
CesA
specific fragment inhibited the endogenous gene expression and it was proved that the cDNA fragment was relevant to the cellulose biosynthesis. Moreover, modulation cellulose synthesis probably was an important influencing factor in polysaccharide metabolism and adaptations of plants to stresses. This will provide technological possibilities for the further study of modulation of the cellulose content of crops. |
| doi_str_mv | 10.1038/srep41397 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5294630</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1865819884</sourcerecordid><originalsourceid>FETCH-LOGICAL-c438t-18b5d5336860c25c793c46593d7194cdf4353705bf542b3d47a918946f34b8543</originalsourceid><addsrcrecordid>eNplkctuEzEUhq2qqK1KF7xAZakbqAj4OmNvkErETVRiA2vL4zmTTOvYqe0B5e3rKCUK1Bsf6XznP5cfoVeUvKOEq_c5wVpQrtsjdMaIkDPGGTs-iE_RRc53pD7JtKD6BJ0yRRvNNDlDm-8huvs-_gk4DthiB95PPmbAeRPK0tZgAQHwxzjOId9gOwzgSsZlCdiDrSXBlrjavD2odDEUCKWmepytL7hED8kGB3gMuEvRuejHl-jFYH2Gi6f_HP36_Onn_Ovs9seXb_Ob25kTXJUZVZ3sJeeNaohj0rWaO9FIzfuWauH6QXDJWyK7QQrW8V60VlOlRTNw0Skp-Dn6sNNdT90KelcnS9abdRpXNm1MtKP5NxPGpVnE32Z7rYaTKvD6SSDFhwlyMasxb7e1AeKUDVWNVFQrte119R96F6cU6nqGakJbQuoalXqzo1yKudo37IehxGw9NXtPK3t5OP2e_OtgBa53QK6psIB00PKZ2iN7WatH</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1901700686</pqid></control><display><type>article</type><title>Knockdown of a cellulose synthase gene BoiCesA affects the leaf anatomy, cellulose content and salt tolerance in broccoli</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Springer Nature OA Free Journals</source><source>Nature Free</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Li, Shuangtao ; Zhang, Lei ; Wang, Ying ; Xu, Fengfeng ; Liu, Mengyun ; Lin, Peng ; Ren, Shuxin ; Ma, Rui ; Guo, Yang-Dong</creator><creatorcontrib>Li, Shuangtao ; Zhang, Lei ; Wang, Ying ; Xu, Fengfeng ; Liu, Mengyun ; Lin, Peng ; Ren, Shuxin ; Ma, Rui ; Guo, Yang-Dong</creatorcontrib><description>Cellulose is the major component of cell wall materials. A 300 bp specific fragment from the cDNA fragment was chosen to insert into vector pFGC1008 at forward and reverse orientations to construct the recombinant RNAi vector. Knockdown of
BoiCesA
caused “dwarf” phenotype with smaller leaves and a loss of the content of cellulose. Moreover, RT-PCR analysis confirmed that the expression of the RNAi apparatus could repress expression of the
CesA
gene. Meanwhile, examination of the leaves from the T3 of RNAi transformants indicated reduction of cell expansion in vascular bundles, particularly on their abaxial surface. The proline and soluble sugar content increased contrarily. Under the salt stress, the T3 of RNAi plants showed significant higher resistance. The expression levels of some salt tolerance related genes (
BoiProH, BoiPIP2;2, BoiPIP2;3
) were significantly changed in T3 of RNAi plants. The results showed that the hairpin structure of
CesA
specific fragment inhibited the endogenous gene expression and it was proved that the cDNA fragment was relevant to the cellulose biosynthesis. Moreover, modulation cellulose synthesis probably was an important influencing factor in polysaccharide metabolism and adaptations of plants to stresses. This will provide technological possibilities for the further study of modulation of the cellulose content of crops.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep41397</identifier><identifier>PMID: 28169290</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>38/77 ; 42/44 ; 42/89 ; 45/22 ; 45/23 ; 631/449/2661/2665 ; 631/449/448/1365 ; Adaptation ; Adaptation, Physiological - drug effects ; Adaptation, Physiological - genetics ; Amino Acid Sequence ; Anatomy ; Brassica - drug effects ; Brassica - enzymology ; Brassica - genetics ; Brassica - physiology ; Cell Wall - metabolism ; Cell walls ; Cellulose ; Cellulose - metabolism ; Cellulose synthase ; CesA gene ; Cloning, Molecular ; DNA, Complementary - genetics ; Gene expression ; Gene Expression Profiling ; Gene Expression Regulation, Plant ; Gene Knockdown Techniques ; Genes, Plant ; Glucosyltransferases - chemistry ; Glucosyltransferases - genetics ; Glucosyltransferases - metabolism ; Humanities and Social Sciences ; Leaves ; multidisciplinary ; Organ Specificity - genetics ; Pectins - metabolism ; Phenotype ; Plant Leaves - anatomy & histology ; Plant Leaves - drug effects ; Plant Leaves - ultrastructure ; Plants, Genetically Modified ; Polymerase chain reaction ; Proline ; Proline - metabolism ; RNA Interference ; RNA-mediated interference ; Science ; Sequence Analysis, DNA ; Sodium Chloride - pharmacology ; Solubility ; Sugar ; Sugars - metabolism ; Transcription, Genetic</subject><ispartof>Scientific reports, 2017-02, Vol.7 (1), p.41397-41397, Article 41397</ispartof><rights>The Author(s) 2017</rights><rights>Copyright Nature Publishing Group Feb 2017</rights><rights>Copyright © 2017, The Author(s) 2017 The Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-18b5d5336860c25c793c46593d7194cdf4353705bf542b3d47a918946f34b8543</citedby><cites>FETCH-LOGICAL-c438t-18b5d5336860c25c793c46593d7194cdf4353705bf542b3d47a918946f34b8543</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5294630/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5294630/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,41120,42189,51576,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28169290$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Shuangtao</creatorcontrib><creatorcontrib>Zhang, Lei</creatorcontrib><creatorcontrib>Wang, Ying</creatorcontrib><creatorcontrib>Xu, Fengfeng</creatorcontrib><creatorcontrib>Liu, Mengyun</creatorcontrib><creatorcontrib>Lin, Peng</creatorcontrib><creatorcontrib>Ren, Shuxin</creatorcontrib><creatorcontrib>Ma, Rui</creatorcontrib><creatorcontrib>Guo, Yang-Dong</creatorcontrib><title>Knockdown of a cellulose synthase gene BoiCesA affects the leaf anatomy, cellulose content and salt tolerance in broccoli</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Cellulose is the major component of cell wall materials. A 300 bp specific fragment from the cDNA fragment was chosen to insert into vector pFGC1008 at forward and reverse orientations to construct the recombinant RNAi vector. Knockdown of
BoiCesA
caused “dwarf” phenotype with smaller leaves and a loss of the content of cellulose. Moreover, RT-PCR analysis confirmed that the expression of the RNAi apparatus could repress expression of the
CesA
gene. Meanwhile, examination of the leaves from the T3 of RNAi transformants indicated reduction of cell expansion in vascular bundles, particularly on their abaxial surface. The proline and soluble sugar content increased contrarily. Under the salt stress, the T3 of RNAi plants showed significant higher resistance. The expression levels of some salt tolerance related genes (
BoiProH, BoiPIP2;2, BoiPIP2;3
) were significantly changed in T3 of RNAi plants. The results showed that the hairpin structure of
CesA
specific fragment inhibited the endogenous gene expression and it was proved that the cDNA fragment was relevant to the cellulose biosynthesis. Moreover, modulation cellulose synthesis probably was an important influencing factor in polysaccharide metabolism and adaptations of plants to stresses. This will provide technological possibilities for the further study of modulation of the cellulose content of crops.</description><subject>38/77</subject><subject>42/44</subject><subject>42/89</subject><subject>45/22</subject><subject>45/23</subject><subject>631/449/2661/2665</subject><subject>631/449/448/1365</subject><subject>Adaptation</subject><subject>Adaptation, Physiological - drug effects</subject><subject>Adaptation, Physiological - genetics</subject><subject>Amino Acid Sequence</subject><subject>Anatomy</subject><subject>Brassica - drug effects</subject><subject>Brassica - enzymology</subject><subject>Brassica - genetics</subject><subject>Brassica - physiology</subject><subject>Cell Wall - metabolism</subject><subject>Cell walls</subject><subject>Cellulose</subject><subject>Cellulose - metabolism</subject><subject>Cellulose synthase</subject><subject>CesA gene</subject><subject>Cloning, Molecular</subject><subject>DNA, Complementary - genetics</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Plant</subject><subject>Gene Knockdown Techniques</subject><subject>Genes, Plant</subject><subject>Glucosyltransferases - chemistry</subject><subject>Glucosyltransferases - genetics</subject><subject>Glucosyltransferases - metabolism</subject><subject>Humanities and Social Sciences</subject><subject>Leaves</subject><subject>multidisciplinary</subject><subject>Organ Specificity - genetics</subject><subject>Pectins - metabolism</subject><subject>Phenotype</subject><subject>Plant Leaves - anatomy & histology</subject><subject>Plant Leaves - drug effects</subject><subject>Plant Leaves - ultrastructure</subject><subject>Plants, Genetically Modified</subject><subject>Polymerase chain reaction</subject><subject>Proline</subject><subject>Proline - metabolism</subject><subject>RNA Interference</subject><subject>RNA-mediated interference</subject><subject>Science</subject><subject>Sequence Analysis, DNA</subject><subject>Sodium Chloride - pharmacology</subject><subject>Solubility</subject><subject>Sugar</subject><subject>Sugars - metabolism</subject><subject>Transcription, Genetic</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNplkctuEzEUhq2qqK1KF7xAZakbqAj4OmNvkErETVRiA2vL4zmTTOvYqe0B5e3rKCUK1Bsf6XznP5cfoVeUvKOEq_c5wVpQrtsjdMaIkDPGGTs-iE_RRc53pD7JtKD6BJ0yRRvNNDlDm-8huvs-_gk4DthiB95PPmbAeRPK0tZgAQHwxzjOId9gOwzgSsZlCdiDrSXBlrjavD2odDEUCKWmepytL7hED8kGB3gMuEvRuejHl-jFYH2Gi6f_HP36_Onn_Ovs9seXb_Ob25kTXJUZVZ3sJeeNaohj0rWaO9FIzfuWauH6QXDJWyK7QQrW8V60VlOlRTNw0Skp-Dn6sNNdT90KelcnS9abdRpXNm1MtKP5NxPGpVnE32Z7rYaTKvD6SSDFhwlyMasxb7e1AeKUDVWNVFQrte119R96F6cU6nqGakJbQuoalXqzo1yKudo37IehxGw9NXtPK3t5OP2e_OtgBa53QK6psIB00PKZ2iN7WatH</recordid><startdate>20170207</startdate><enddate>20170207</enddate><creator>Li, Shuangtao</creator><creator>Zhang, Lei</creator><creator>Wang, Ying</creator><creator>Xu, Fengfeng</creator><creator>Liu, Mengyun</creator><creator>Lin, Peng</creator><creator>Ren, Shuxin</creator><creator>Ma, Rui</creator><creator>Guo, Yang-Dong</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20170207</creationdate><title>Knockdown of a cellulose synthase gene BoiCesA affects the leaf anatomy, cellulose content and salt tolerance in broccoli</title><author>Li, Shuangtao ; Zhang, Lei ; Wang, Ying ; Xu, Fengfeng ; Liu, Mengyun ; Lin, Peng ; Ren, Shuxin ; Ma, Rui ; Guo, Yang-Dong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-18b5d5336860c25c793c46593d7194cdf4353705bf542b3d47a918946f34b8543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>38/77</topic><topic>42/44</topic><topic>42/89</topic><topic>45/22</topic><topic>45/23</topic><topic>631/449/2661/2665</topic><topic>631/449/448/1365</topic><topic>Adaptation</topic><topic>Adaptation, Physiological - drug effects</topic><topic>Adaptation, Physiological - genetics</topic><topic>Amino Acid Sequence</topic><topic>Anatomy</topic><topic>Brassica - drug effects</topic><topic>Brassica - enzymology</topic><topic>Brassica - genetics</topic><topic>Brassica - physiology</topic><topic>Cell Wall - metabolism</topic><topic>Cell walls</topic><topic>Cellulose</topic><topic>Cellulose - metabolism</topic><topic>Cellulose synthase</topic><topic>CesA gene</topic><topic>Cloning, Molecular</topic><topic>DNA, Complementary - genetics</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Plant</topic><topic>Gene Knockdown Techniques</topic><topic>Genes, Plant</topic><topic>Glucosyltransferases - chemistry</topic><topic>Glucosyltransferases - genetics</topic><topic>Glucosyltransferases - metabolism</topic><topic>Humanities and Social Sciences</topic><topic>Leaves</topic><topic>multidisciplinary</topic><topic>Organ Specificity - genetics</topic><topic>Pectins - metabolism</topic><topic>Phenotype</topic><topic>Plant Leaves - anatomy & histology</topic><topic>Plant Leaves - drug effects</topic><topic>Plant Leaves - ultrastructure</topic><topic>Plants, Genetically Modified</topic><topic>Polymerase chain reaction</topic><topic>Proline</topic><topic>Proline - metabolism</topic><topic>RNA Interference</topic><topic>RNA-mediated interference</topic><topic>Science</topic><topic>Sequence Analysis, DNA</topic><topic>Sodium Chloride - pharmacology</topic><topic>Solubility</topic><topic>Sugar</topic><topic>Sugars - metabolism</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Shuangtao</creatorcontrib><creatorcontrib>Zhang, Lei</creatorcontrib><creatorcontrib>Wang, Ying</creatorcontrib><creatorcontrib>Xu, Fengfeng</creatorcontrib><creatorcontrib>Liu, Mengyun</creatorcontrib><creatorcontrib>Lin, Peng</creatorcontrib><creatorcontrib>Ren, Shuxin</creatorcontrib><creatorcontrib>Ma, Rui</creatorcontrib><creatorcontrib>Guo, Yang-Dong</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Shuangtao</au><au>Zhang, Lei</au><au>Wang, Ying</au><au>Xu, Fengfeng</au><au>Liu, Mengyun</au><au>Lin, Peng</au><au>Ren, Shuxin</au><au>Ma, Rui</au><au>Guo, Yang-Dong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Knockdown of a cellulose synthase gene BoiCesA affects the leaf anatomy, cellulose content and salt tolerance in broccoli</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2017-02-07</date><risdate>2017</risdate><volume>7</volume><issue>1</issue><spage>41397</spage><epage>41397</epage><pages>41397-41397</pages><artnum>41397</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Cellulose is the major component of cell wall materials. A 300 bp specific fragment from the cDNA fragment was chosen to insert into vector pFGC1008 at forward and reverse orientations to construct the recombinant RNAi vector. Knockdown of
BoiCesA
caused “dwarf” phenotype with smaller leaves and a loss of the content of cellulose. Moreover, RT-PCR analysis confirmed that the expression of the RNAi apparatus could repress expression of the
CesA
gene. Meanwhile, examination of the leaves from the T3 of RNAi transformants indicated reduction of cell expansion in vascular bundles, particularly on their abaxial surface. The proline and soluble sugar content increased contrarily. Under the salt stress, the T3 of RNAi plants showed significant higher resistance. The expression levels of some salt tolerance related genes (
BoiProH, BoiPIP2;2, BoiPIP2;3
) were significantly changed in T3 of RNAi plants. The results showed that the hairpin structure of
CesA
specific fragment inhibited the endogenous gene expression and it was proved that the cDNA fragment was relevant to the cellulose biosynthesis. Moreover, modulation cellulose synthesis probably was an important influencing factor in polysaccharide metabolism and adaptations of plants to stresses. This will provide technological possibilities for the further study of modulation of the cellulose content of crops.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>28169290</pmid><doi>10.1038/srep41397</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 38/77 42/44 42/89 45/22 45/23 631/449/2661/2665 631/449/448/1365 Adaptation Adaptation, Physiological - drug effects Adaptation, Physiological - genetics Amino Acid Sequence Anatomy Brassica - drug effects Brassica - enzymology Brassica - genetics Brassica - physiology Cell Wall - metabolism Cell walls Cellulose Cellulose - metabolism Cellulose synthase CesA gene Cloning, Molecular DNA, Complementary - genetics Gene expression Gene Expression Profiling Gene Expression Regulation, Plant Gene Knockdown Techniques Genes, Plant Glucosyltransferases - chemistry Glucosyltransferases - genetics Glucosyltransferases - metabolism Humanities and Social Sciences Leaves multidisciplinary Organ Specificity - genetics Pectins - metabolism Phenotype Plant Leaves - anatomy & histology Plant Leaves - drug effects Plant Leaves - ultrastructure Plants, Genetically Modified Polymerase chain reaction Proline Proline - metabolism RNA Interference RNA-mediated interference Science Sequence Analysis, DNA Sodium Chloride - pharmacology Solubility Sugar Sugars - metabolism Transcription, Genetic |
| title | Knockdown of a cellulose synthase gene BoiCesA affects the leaf anatomy, cellulose content and salt tolerance in broccoli |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T20%3A06%3A38IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Knockdown%20of%20a%20cellulose%20synthase%20gene%20BoiCesA%20affects%20the%20leaf%20anatomy,%20cellulose%20content%20and%20salt%20tolerance%20in%20broccoli&rft.jtitle=Scientific%20reports&rft.au=Li,%20Shuangtao&rft.date=2017-02-07&rft.volume=7&rft.issue=1&rft.spage=41397&rft.epage=41397&rft.pages=41397-41397&rft.artnum=41397&rft.issn=2045-2322&rft.eissn=2045-2322&rft_id=info:doi/10.1038/srep41397&rft_dat=%3Cproquest_pubme%3E1865819884%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1901700686&rft_id=info:pmid/28169290&rfr_iscdi=true |