Honeybee locomotion is impaired by Am-CaV3 low voltage-activated Ca2+ channel antagonist
Voltage‐gated Ca 2+ channels are key transducers of cellular excitability and participate in several crucial physiological responses. In vertebrates, 10 Ca 2+ channel genes, grouped in 3 families ( Ca V 1, Ca V 2 and Ca V 3 ), have been described and characterized. Insects possess only one member of...
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| Veröffentlicht in: | Scientific reports 2017-02, Vol.7 (1), p.41782, Article 41782 |
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| creator | Rousset, M. Collet, C. Cens, T. Bastin, F. Raymond, V. Massou, I. Menard, C. Thibaud, J.-B. Charreton, M. Vignes, M. Chahine, M. Sandoz, J. C. Charnet, P. |
| description | Voltage‐gated Ca
2+
channels are key transducers of cellular excitability and participate in several crucial physiological responses. In vertebrates, 10 Ca
2+
channel genes, grouped in 3 families (
Ca
V
1, Ca
V
2
and
Ca
V
3
), have been described and characterized. Insects possess only one member of each family. These genes have been isolated in a limited number of species and very few have been characterized although, in addition to their crucial role, they may represent a collateral target for neurotoxic insecticides. We have isolated the 3 genes coding for the 3 Ca
2+
channels expressed in
Apis mellifera
. This work provides the first detailed characterization of the honeybee T-type Ca
V
3 Ca
2+
channel and demonstrates the low toxicity of inhibiting this channel. Comparing Ca
2+
currents recorded in bee neurons and myocytes with Ca
2+
currents recorded in Xenopus oocytes expressing the honeybee
Ca
V
3
gene suggests native expression in bee muscle cells only. High‐voltage activated Ca
2+
channels could be recorded in the somata of different cultured bee neurons. These functional data were confirmed by
in situ
hybridization, immunolocalization and
in vivo
analysis of the effects of a Ca
V
3 inhibitor. The biophysical and pharmacological characterization and the tissue distribution of Ca
V
3 suggest a role in honeybee muscle function. |
| doi_str_mv | 10.1038/srep41782 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5286435</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1901688017</sourcerecordid><originalsourceid>FETCH-LOGICAL-c345t-26bcb74b9a69c811f28b52e1437f2eb8bb5d33d974d5644392bd1aefaea36a573</originalsourceid><addsrcrecordid>eNplkU9LxDAQxYMoKqsHv0HBk0o1f9v0IkhRVxC8qHgLkzZdI22yJt2V_fZm2UUU5zID78ebYR5CJwRfEszkVQxmzkkp6Q46pJiLnDJKd3_NB-g4xg-cStCKk2ofHVBJuBCYH6K3qXdmpY3Jet_4wY_Wu8zGzA5zsMG0mV5lN0NewytLxFe29P0IM5NDM9oljAmogV5kzTs4Z_oMXFK9s3E8Qnsd9NEcb_sEvdzdPtfT_PHp_qG-ecwbxsWY00I3uuS6gqJqJCEdlVpQQzgrO2q01Fq0jLVVyVtRcM4qqlsCpgMDrABRsgm63vjOF3owbWPcGKBX82AHCCvlwaq_irPvauaXSlBZcCaSwenWIPjPhYmj-vCL4NLNilSYFFJisl5ztqGa4GN6efezgWC1zkH95JDY8w0bE-NmJvxy_Ad_A7jeiH8</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1901688017</pqid></control><display><type>article</type><title>Honeybee locomotion is impaired by Am-CaV3 low voltage-activated Ca2+ channel antagonist</title><source>Nature Free</source><source>DOAJ Directory of Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><source>Springer Nature OA Free Journals</source><creator>Rousset, M. ; Collet, C. ; Cens, T. ; Bastin, F. ; Raymond, V. ; Massou, I. ; Menard, C. ; Thibaud, J.-B. ; Charreton, M. ; Vignes, M. ; Chahine, M. ; Sandoz, J. C. ; Charnet, P.</creator><creatorcontrib>Rousset, M. ; Collet, C. ; Cens, T. ; Bastin, F. ; Raymond, V. ; Massou, I. ; Menard, C. ; Thibaud, J.-B. ; Charreton, M. ; Vignes, M. ; Chahine, M. ; Sandoz, J. C. ; Charnet, P.</creatorcontrib><description>Voltage‐gated Ca
2+
channels are key transducers of cellular excitability and participate in several crucial physiological responses. In vertebrates, 10 Ca
2+
channel genes, grouped in 3 families (
Ca
V
1, Ca
V
2
and
Ca
V
3
), have been described and characterized. Insects possess only one member of each family. These genes have been isolated in a limited number of species and very few have been characterized although, in addition to their crucial role, they may represent a collateral target for neurotoxic insecticides. We have isolated the 3 genes coding for the 3 Ca
2+
channels expressed in
Apis mellifera
. This work provides the first detailed characterization of the honeybee T-type Ca
V
3 Ca
2+
channel and demonstrates the low toxicity of inhibiting this channel. Comparing Ca
2+
currents recorded in bee neurons and myocytes with Ca
2+
currents recorded in Xenopus oocytes expressing the honeybee
Ca
V
3
gene suggests native expression in bee muscle cells only. High‐voltage activated Ca
2+
channels could be recorded in the somata of different cultured bee neurons. These functional data were confirmed by
in situ
hybridization, immunolocalization and
in vivo
analysis of the effects of a Ca
V
3 inhibitor. The biophysical and pharmacological characterization and the tissue distribution of Ca
V
3 suggest a role in honeybee muscle function.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep41782</identifier><identifier>PMID: 28145504</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13/106 ; 14/63 ; 631/378/340 ; 631/92/436 ; 64/114 ; 9/74 ; Bees ; Calcium channels ; Calcium channels (T-type) ; Calcium channels (voltage-gated) ; Caveolin-1 ; Excitability ; Humanities and Social Sciences ; Insecticides ; Locomotion ; multidisciplinary ; Myocytes ; Neural coding ; Neurotoxicity ; Oocytes ; Physiological responses ; Science ; Science (multidisciplinary) ; Toxicity ; Transducers</subject><ispartof>Scientific reports, 2017-02, Vol.7 (1), p.41782, Article 41782</ispartof><rights>The Author(s) 2017</rights><rights>Copyright Nature Publishing Group Feb 2017</rights><rights>Copyright © 2017, The Author(s) 2017 The Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c345t-26bcb74b9a69c811f28b52e1437f2eb8bb5d33d974d5644392bd1aefaea36a573</citedby><cites>FETCH-LOGICAL-c345t-26bcb74b9a69c811f28b52e1437f2eb8bb5d33d974d5644392bd1aefaea36a573</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5286435/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5286435/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,41096,42165,51551,53766,53768</link.rule.ids></links><search><creatorcontrib>Rousset, M.</creatorcontrib><creatorcontrib>Collet, C.</creatorcontrib><creatorcontrib>Cens, T.</creatorcontrib><creatorcontrib>Bastin, F.</creatorcontrib><creatorcontrib>Raymond, V.</creatorcontrib><creatorcontrib>Massou, I.</creatorcontrib><creatorcontrib>Menard, C.</creatorcontrib><creatorcontrib>Thibaud, J.-B.</creatorcontrib><creatorcontrib>Charreton, M.</creatorcontrib><creatorcontrib>Vignes, M.</creatorcontrib><creatorcontrib>Chahine, M.</creatorcontrib><creatorcontrib>Sandoz, J. C.</creatorcontrib><creatorcontrib>Charnet, P.</creatorcontrib><title>Honeybee locomotion is impaired by Am-CaV3 low voltage-activated Ca2+ channel antagonist</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><description>Voltage‐gated Ca
2+
channels are key transducers of cellular excitability and participate in several crucial physiological responses. In vertebrates, 10 Ca
2+
channel genes, grouped in 3 families (
Ca
V
1, Ca
V
2
and
Ca
V
3
), have been described and characterized. Insects possess only one member of each family. These genes have been isolated in a limited number of species and very few have been characterized although, in addition to their crucial role, they may represent a collateral target for neurotoxic insecticides. We have isolated the 3 genes coding for the 3 Ca
2+
channels expressed in
Apis mellifera
. This work provides the first detailed characterization of the honeybee T-type Ca
V
3 Ca
2+
channel and demonstrates the low toxicity of inhibiting this channel. Comparing Ca
2+
currents recorded in bee neurons and myocytes with Ca
2+
currents recorded in Xenopus oocytes expressing the honeybee
Ca
V
3
gene suggests native expression in bee muscle cells only. High‐voltage activated Ca
2+
channels could be recorded in the somata of different cultured bee neurons. These functional data were confirmed by
in situ
hybridization, immunolocalization and
in vivo
analysis of the effects of a Ca
V
3 inhibitor. The biophysical and pharmacological characterization and the tissue distribution of Ca
V
3 suggest a role in honeybee muscle function.</description><subject>13/106</subject><subject>14/63</subject><subject>631/378/340</subject><subject>631/92/436</subject><subject>64/114</subject><subject>9/74</subject><subject>Bees</subject><subject>Calcium channels</subject><subject>Calcium channels (T-type)</subject><subject>Calcium channels (voltage-gated)</subject><subject>Caveolin-1</subject><subject>Excitability</subject><subject>Humanities and Social Sciences</subject><subject>Insecticides</subject><subject>Locomotion</subject><subject>multidisciplinary</subject><subject>Myocytes</subject><subject>Neural coding</subject><subject>Neurotoxicity</subject><subject>Oocytes</subject><subject>Physiological responses</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Toxicity</subject><subject>Transducers</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>BENPR</sourceid><recordid>eNplkU9LxDAQxYMoKqsHv0HBk0o1f9v0IkhRVxC8qHgLkzZdI22yJt2V_fZm2UUU5zID78ebYR5CJwRfEszkVQxmzkkp6Q46pJiLnDJKd3_NB-g4xg-cStCKk2ofHVBJuBCYH6K3qXdmpY3Jet_4wY_Wu8zGzA5zsMG0mV5lN0NewytLxFe29P0IM5NDM9oljAmogV5kzTs4Z_oMXFK9s3E8Qnsd9NEcb_sEvdzdPtfT_PHp_qG-ecwbxsWY00I3uuS6gqJqJCEdlVpQQzgrO2q01Fq0jLVVyVtRcM4qqlsCpgMDrABRsgm63vjOF3owbWPcGKBX82AHCCvlwaq_irPvauaXSlBZcCaSwenWIPjPhYmj-vCL4NLNilSYFFJisl5ztqGa4GN6efezgWC1zkH95JDY8w0bE-NmJvxy_Ad_A7jeiH8</recordid><startdate>20170201</startdate><enddate>20170201</enddate><creator>Rousset, M.</creator><creator>Collet, C.</creator><creator>Cens, T.</creator><creator>Bastin, F.</creator><creator>Raymond, V.</creator><creator>Massou, I.</creator><creator>Menard, C.</creator><creator>Thibaud, J.-B.</creator><creator>Charreton, M.</creator><creator>Vignes, M.</creator><creator>Chahine, M.</creator><creator>Sandoz, J. C.</creator><creator>Charnet, P.</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>5PM</scope></search><sort><creationdate>20170201</creationdate><title>Honeybee locomotion is impaired by Am-CaV3 low voltage-activated Ca2+ channel antagonist</title><author>Rousset, M. ; Collet, C. ; Cens, T. ; Bastin, F. ; Raymond, V. ; Massou, I. ; Menard, C. ; Thibaud, J.-B. ; Charreton, M. ; Vignes, M. ; Chahine, M. ; Sandoz, J. C. ; Charnet, P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c345t-26bcb74b9a69c811f28b52e1437f2eb8bb5d33d974d5644392bd1aefaea36a573</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>13/106</topic><topic>14/63</topic><topic>631/378/340</topic><topic>631/92/436</topic><topic>64/114</topic><topic>9/74</topic><topic>Bees</topic><topic>Calcium channels</topic><topic>Calcium channels (T-type)</topic><topic>Calcium channels (voltage-gated)</topic><topic>Caveolin-1</topic><topic>Excitability</topic><topic>Humanities and Social Sciences</topic><topic>Insecticides</topic><topic>Locomotion</topic><topic>multidisciplinary</topic><topic>Myocytes</topic><topic>Neural coding</topic><topic>Neurotoxicity</topic><topic>Oocytes</topic><topic>Physiological responses</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Toxicity</topic><topic>Transducers</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rousset, M.</creatorcontrib><creatorcontrib>Collet, C.</creatorcontrib><creatorcontrib>Cens, T.</creatorcontrib><creatorcontrib>Bastin, F.</creatorcontrib><creatorcontrib>Raymond, V.</creatorcontrib><creatorcontrib>Massou, I.</creatorcontrib><creatorcontrib>Menard, C.</creatorcontrib><creatorcontrib>Thibaud, J.-B.</creatorcontrib><creatorcontrib>Charreton, M.</creatorcontrib><creatorcontrib>Vignes, M.</creatorcontrib><creatorcontrib>Chahine, M.</creatorcontrib><creatorcontrib>Sandoz, J. C.</creatorcontrib><creatorcontrib>Charnet, P.</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rousset, M.</au><au>Collet, C.</au><au>Cens, T.</au><au>Bastin, F.</au><au>Raymond, V.</au><au>Massou, I.</au><au>Menard, C.</au><au>Thibaud, J.-B.</au><au>Charreton, M.</au><au>Vignes, M.</au><au>Chahine, M.</au><au>Sandoz, J. C.</au><au>Charnet, P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Honeybee locomotion is impaired by Am-CaV3 low voltage-activated Ca2+ channel antagonist</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><date>2017-02-01</date><risdate>2017</risdate><volume>7</volume><issue>1</issue><spage>41782</spage><pages>41782-</pages><artnum>41782</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Voltage‐gated Ca
2+
channels are key transducers of cellular excitability and participate in several crucial physiological responses. In vertebrates, 10 Ca
2+
channel genes, grouped in 3 families (
Ca
V
1, Ca
V
2
and
Ca
V
3
), have been described and characterized. Insects possess only one member of each family. These genes have been isolated in a limited number of species and very few have been characterized although, in addition to their crucial role, they may represent a collateral target for neurotoxic insecticides. We have isolated the 3 genes coding for the 3 Ca
2+
channels expressed in
Apis mellifera
. This work provides the first detailed characterization of the honeybee T-type Ca
V
3 Ca
2+
channel and demonstrates the low toxicity of inhibiting this channel. Comparing Ca
2+
currents recorded in bee neurons and myocytes with Ca
2+
currents recorded in Xenopus oocytes expressing the honeybee
Ca
V
3
gene suggests native expression in bee muscle cells only. High‐voltage activated Ca
2+
channels could be recorded in the somata of different cultured bee neurons. These functional data were confirmed by
in situ
hybridization, immunolocalization and
in vivo
analysis of the effects of a Ca
V
3 inhibitor. The biophysical and pharmacological characterization and the tissue distribution of Ca
V
3 suggest a role in honeybee muscle function.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>28145504</pmid><doi>10.1038/srep41782</doi><oa>free_for_read</oa></addata></record> |
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| source | Nature Free; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry; Springer Nature OA Free Journals |
| subjects | 13/106 14/63 631/378/340 631/92/436 64/114 9/74 Bees Calcium channels Calcium channels (T-type) Calcium channels (voltage-gated) Caveolin-1 Excitability Humanities and Social Sciences Insecticides Locomotion multidisciplinary Myocytes Neural coding Neurotoxicity Oocytes Physiological responses Science Science (multidisciplinary) Toxicity Transducers |
| title | Honeybee locomotion is impaired by Am-CaV3 low voltage-activated Ca2+ channel antagonist |
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