Efficient Adhesion Culture of Human Pluripotent Stem Cells Using Laminin Fragments in an Uncoated Manner

We describe highly effective adhesion culture of human pluripotent stem cells (hPSCs) using laminin fragments without precoating. Culture substrates have been generally thought to exert a cell adhesion effect when they are precoated onto culture vessels. However, simple addition of laminin fragments...

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Veröffentlicht in:	Scientific reports 2017-01, Vol.7 (1), p.41165-41165, Article 41165
Hauptverfasser:	Miyazaki, Takamichi, Isobe, Takehisa, Nakatsuji, Norio, Suemori, Hirofumi
Format:	Artikel
Sprache:	eng
Schlagworte:	13 13/106 631/532/2064/2117 631/532/2064/2158 Adhesion Cell adhesion Cell adhesion & migration Cell culture Humanities and Social Sciences Laminin multidisciplinary Pluripotency Regenerative medicine Science Science (multidisciplinary) Stem cells Subculture Vitronectin
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