Comparison of two sperm processing techniques for low complexity assisted fertilization: sperm washing followed by swim-up and discontinuous density gradient centrifugation
This study aimed to assess and compare sperm motility, concentration, and morphology recovery rates, before and after processing through sperm washing followed by swim-up or discontinuous density gradient centrifugation in normospermic individuals. Fifty-eight semen samples were used in double intra...
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| Veröffentlicht in: | JBRA assisted reproduction 2016, Vol.20 (4), p.206-211 |
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| creator | Fácio, Cássio L Previato, Lígia F Machado-Paula, Ligiane A Matheus, Paulo Cs Araújo, Filho, Edilberto |
| description | This study aimed to assess and compare sperm motility, concentration, and morphology recovery rates, before and after processing through sperm washing followed by swim-up or discontinuous density gradient centrifugation in normospermic individuals.
Fifty-eight semen samples were used in double intrauterine insemination procedures; 17 samples (group 1) were prepared with sperm washing followed by swim-up, and 41 (group 2) by discontinuous density gradient centrifugation. This prospective non-randomized study assessed seminal parameters before and after semen processing. A dependent t-test was used for the same technique to analyze seminal parameters before and after semen processing; an independent t-test was used to compare the results before and after processing for both techniques.
The two techniques produced decreases in sample concentration (sperm washing followed by swim-up: P |
| doi_str_mv | 10.5935/1518-0557.20160040 |
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Fifty-eight semen samples were used in double intrauterine insemination procedures; 17 samples (group 1) were prepared with sperm washing followed by swim-up, and 41 (group 2) by discontinuous density gradient centrifugation. This prospective non-randomized study assessed seminal parameters before and after semen processing. A dependent t-test was used for the same technique to analyze seminal parameters before and after semen processing; an independent t-test was used to compare the results before and after processing for both techniques.
The two techniques produced decreases in sample concentration (sperm washing followed by swim-up: P<0.000006; discontinuous density gradient centrifugation: P=0.008457) and increases in motility and normal morphology sperm rates after processing. The difference in sperm motility between the two techniques was not statistically significant. Sperm washing followed by swim-up had better morphology recovery rates than discontinuous density gradient centrifugation (P=0.0095); and the density gradient group had better concentration recovery rates than the swim-up group (P=0.0027).
The two methods successfully recovered the minimum sperm values needed to perform intrauterine insemination. Sperm washing followed by swim-up is indicated for semen with high sperm concentration and better morphology recovery rates. Discontinuous density gradient centrifugation produced improved concentration recovery rates.</description><identifier>ISSN: 1518-0557</identifier><identifier>ISSN: 1517-5693</identifier><identifier>EISSN: 1518-0557</identifier><identifier>DOI: 10.5935/1518-0557.20160040</identifier><identifier>PMID: 28050954</identifier><language>eng</language><publisher>Brazil: Sociedade Brasileira de Reprodução Humana (Brazilian Society of Assisted Reproduction)</publisher><subject>Original</subject><ispartof>JBRA assisted reproduction, 2016, Vol.20 (4), p.206-211</ispartof><rights>2017 This work is licensed under the Creative Commons Attribution License (https://creativecommons.org/licenses/by/3.0/) (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2016. This work is licensed under the Creative Commons Attribution License (https://creativecommons.org/licenses/by/3.0/) (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c458t-41949254cfe835b96ab4213d20036d3d249afd474e80e343f5976d595f1afd0a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5265618/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5265618/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,725,778,782,883,4012,27906,27907,27908,53774,53776</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28050954$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fácio, Cássio L</creatorcontrib><creatorcontrib>Previato, Lígia F</creatorcontrib><creatorcontrib>Machado-Paula, Ligiane A</creatorcontrib><creatorcontrib>Matheus, Paulo Cs</creatorcontrib><creatorcontrib>Araújo, Filho, Edilberto</creatorcontrib><title>Comparison of two sperm processing techniques for low complexity assisted fertilization: sperm washing followed by swim-up and discontinuous density gradient centrifugation</title><title>JBRA assisted reproduction</title><addtitle>JBRA Assist Reprod</addtitle><description>This study aimed to assess and compare sperm motility, concentration, and morphology recovery rates, before and after processing through sperm washing followed by swim-up or discontinuous density gradient centrifugation in normospermic individuals.
Fifty-eight semen samples were used in double intrauterine insemination procedures; 17 samples (group 1) were prepared with sperm washing followed by swim-up, and 41 (group 2) by discontinuous density gradient centrifugation. This prospective non-randomized study assessed seminal parameters before and after semen processing. A dependent t-test was used for the same technique to analyze seminal parameters before and after semen processing; an independent t-test was used to compare the results before and after processing for both techniques.
The two techniques produced decreases in sample concentration (sperm washing followed by swim-up: P<0.000006; discontinuous density gradient centrifugation: P=0.008457) and increases in motility and normal morphology sperm rates after processing. The difference in sperm motility between the two techniques was not statistically significant. Sperm washing followed by swim-up had better morphology recovery rates than discontinuous density gradient centrifugation (P=0.0095); and the density gradient group had better concentration recovery rates than the swim-up group (P=0.0027).
The two methods successfully recovered the minimum sperm values needed to perform intrauterine insemination. Sperm washing followed by swim-up is indicated for semen with high sperm concentration and better morphology recovery rates. Discontinuous density gradient centrifugation produced improved concentration recovery rates.</description><subject>Original</subject><issn>1518-0557</issn><issn>1517-5693</issn><issn>1518-0557</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNp9Ustu1DAUjRCIVqU_wAJZYsMmxa-bxCyQ0IiXVKmbsrY8sT3jKrGD7TAM38RH4rTTUemiG1_r-pyje65PVb0m-AIEg_cESFdjgPaCYtJgzPGz6vTYfP7gflKdp3SDMSaCUMbxy-qEdhiwAH5a_V2FcVLRpeBRsCjvAkqTiSOaYuhNSs5vUDb91rufs0nIhoiGsEN9YQ3mt8t7pAooZaORNTG7wf1R2QX_4SCzU2m7aNgwFF5Brfco7dxYzxNSXiPtUh98dn4Oc0La-LRobqLSzviM-nJEZ-fNreir6oVVQzLnh3pW_fjy-Xr1rb68-vp99emy7jl0ueZEcEGB99Z0DNaiUWtOCdMUY9boUrlQVvOWmw4bxpkF0TYaBFhS-lixs-rjne40r0ejb4dQg5yiG1Xcy6Cc_P_Fu63chF8SaAMN6YrAu4NADMveshyLTzMMypviU5IOgDHStqxA3z6C3oQ5-mJPEgGEtqJM_TSKEGhZ-c6ConeoPoaUorHHkQmWS2rkEgq5hELep6aQ3jw0e6TcZ4T9A35Cwag</recordid><startdate>2016</startdate><enddate>2016</enddate><creator>Fácio, Cássio L</creator><creator>Previato, Lígia F</creator><creator>Machado-Paula, Ligiane A</creator><creator>Matheus, Paulo Cs</creator><creator>Araújo, Filho, Edilberto</creator><general>Sociedade Brasileira de Reprodução Humana (Brazilian Society of Assisted Reproduction)</general><general>Brazilian Society of Assisted Reproduction</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>2016</creationdate><title>Comparison of two sperm processing techniques for low complexity assisted fertilization: sperm washing followed by swim-up and discontinuous density gradient centrifugation</title><author>Fácio, Cássio L ; Previato, Lígia F ; Machado-Paula, Ligiane A ; Matheus, Paulo Cs ; Araújo, Filho, Edilberto</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c458t-41949254cfe835b96ab4213d20036d3d249afd474e80e343f5976d595f1afd0a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Original</topic><toplevel>online_resources</toplevel><creatorcontrib>Fácio, Cássio L</creatorcontrib><creatorcontrib>Previato, Lígia F</creatorcontrib><creatorcontrib>Machado-Paula, Ligiane A</creatorcontrib><creatorcontrib>Matheus, Paulo Cs</creatorcontrib><creatorcontrib>Araújo, Filho, Edilberto</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>JBRA assisted reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fácio, Cássio L</au><au>Previato, Lígia F</au><au>Machado-Paula, Ligiane A</au><au>Matheus, Paulo Cs</au><au>Araújo, Filho, Edilberto</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of two sperm processing techniques for low complexity assisted fertilization: sperm washing followed by swim-up and discontinuous density gradient centrifugation</atitle><jtitle>JBRA assisted reproduction</jtitle><addtitle>JBRA Assist Reprod</addtitle><date>2016</date><risdate>2016</risdate><volume>20</volume><issue>4</issue><spage>206</spage><epage>211</epage><pages>206-211</pages><issn>1518-0557</issn><issn>1517-5693</issn><eissn>1518-0557</eissn><abstract>This study aimed to assess and compare sperm motility, concentration, and morphology recovery rates, before and after processing through sperm washing followed by swim-up or discontinuous density gradient centrifugation in normospermic individuals.
Fifty-eight semen samples were used in double intrauterine insemination procedures; 17 samples (group 1) were prepared with sperm washing followed by swim-up, and 41 (group 2) by discontinuous density gradient centrifugation. This prospective non-randomized study assessed seminal parameters before and after semen processing. A dependent t-test was used for the same technique to analyze seminal parameters before and after semen processing; an independent t-test was used to compare the results before and after processing for both techniques.
The two techniques produced decreases in sample concentration (sperm washing followed by swim-up: P<0.000006; discontinuous density gradient centrifugation: P=0.008457) and increases in motility and normal morphology sperm rates after processing. The difference in sperm motility between the two techniques was not statistically significant. Sperm washing followed by swim-up had better morphology recovery rates than discontinuous density gradient centrifugation (P=0.0095); and the density gradient group had better concentration recovery rates than the swim-up group (P=0.0027).
The two methods successfully recovered the minimum sperm values needed to perform intrauterine insemination. Sperm washing followed by swim-up is indicated for semen with high sperm concentration and better morphology recovery rates. Discontinuous density gradient centrifugation produced improved concentration recovery rates.</abstract><cop>Brazil</cop><pub>Sociedade Brasileira de Reprodução Humana (Brazilian Society of Assisted Reproduction)</pub><pmid>28050954</pmid><doi>10.5935/1518-0557.20160040</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| title | Comparison of two sperm processing techniques for low complexity assisted fertilization: sperm washing followed by swim-up and discontinuous density gradient centrifugation |
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