Selection of chemically defined media for CHO cell fed-batch culture processes
Two CHO cell clones derived from the same parental CHO BC® cell line and producing the same monoclonal antibody (BC-G, a low producing clone; BC-P, a high producing clone) were tested in four basal media in all possible combinations with three feeds (=12 conditions) in fed-batch cultures. Higher ami...
Gespeichert in:
| Veröffentlicht in: | Cytotechnology (Dordrecht) 2017-02, Vol.69 (1), p.39-56 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 56 |
|---|---|
| container_issue | 1 |
| container_start_page | 39 |
| container_title | Cytotechnology (Dordrecht) |
| container_volume | 69 |
| creator | Pan, Xiao Streefland, Mathieu Dalm, Ciska Wijffels, René H. Martens, Dirk E. |
| description | Two CHO cell clones derived from the same parental CHO
BC®
cell line and producing the same monoclonal antibody (BC-G, a low producing clone; BC-P, a high producing clone) were tested in four basal media in all possible combinations with three feeds (=12 conditions) in fed-batch cultures. Higher amino acid feeding did not always lead to higher mAb production. The two clones showed differences in cell physiology, metabolism and optimal medium-feed combinations. During the phase transitions of all cultures, cell metabolism showed a shift represented by lower specific consumption and production rates, except for the specific glucose consumption rate in cultures fed by Actifeed A/B. The BC-P clone fed by Actifeed A/B showed a threefold cell volume increase and an increase of the specific consumption rate of glucose in the stationary phase. Since feeding was based on glucose this resulted in accumulation of amino acids for this feed, while this did not occur for the poorer feed (EFA/B). The same feed also led to an increase of cell size for the BC-G clone, but to a lesser extent. |
| doi_str_mv | 10.1007/s10616-016-0036-5 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5264622</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1880792288</sourcerecordid><originalsourceid>FETCH-LOGICAL-c601t-e3ef4727f734cb5e51c30bca4d15b55532cf6c93afef7df2b6a259fbd86e5abf3</originalsourceid><addsrcrecordid>eNp9kUFv1DAQhS1ERbeFH8AFWeLCJXQ8ie34goRWtEWq6AE4W44z7qbKxoudVOq_x6ttq4IEh9Ec5ps38_QYeyvgowDQZ1mAEqqCfUGtKvmCrYTUdQVaty_ZCgxCZUCZY3aS8y0AGC3qV-wYtQFQqFbs23cayc9DnHgM3G9oO3g3jve8pzBM1PMt9YPjISa-vrzmnsaRB-qrzs1-w_0yzksivkvRU86UX7Oj4MZMbx76Kft5_uXH-rK6ur74uv58VXkFYq6optBo1EHXje8kSeFr6LxreiE7KWWNPihvahco6D5gpxxKE7q-VSRdF-pT9umgu1u68qGnaU5utLs0bF26t9EN9s_JNGzsTbyzElWjEIvAhweBFH8tlGe7HfLenZsoLtmKtpHYtC2Ygr7_C72NS5qKPYtGtCgRGvwfJYqMNohtWyhxoHyKOScKTy8LsPtM7SFTC_sqmVpZdt499_q08RhiAfAA5DKabig9O_1P1d-0Yax5</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2918252042</pqid></control><display><type>article</type><title>Selection of chemically defined media for CHO cell fed-batch culture processes</title><source>SpringerNature Complete Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>ProQuest Central UK/Ireland</source><source>PubMed Central</source><source>ProQuest Central</source><creator>Pan, Xiao ; Streefland, Mathieu ; Dalm, Ciska ; Wijffels, René H. ; Martens, Dirk E.</creator><creatorcontrib>Pan, Xiao ; Streefland, Mathieu ; Dalm, Ciska ; Wijffels, René H. ; Martens, Dirk E.</creatorcontrib><description>Two CHO cell clones derived from the same parental CHO
BC®
cell line and producing the same monoclonal antibody (BC-G, a low producing clone; BC-P, a high producing clone) were tested in four basal media in all possible combinations with three feeds (=12 conditions) in fed-batch cultures. Higher amino acid feeding did not always lead to higher mAb production. The two clones showed differences in cell physiology, metabolism and optimal medium-feed combinations. During the phase transitions of all cultures, cell metabolism showed a shift represented by lower specific consumption and production rates, except for the specific glucose consumption rate in cultures fed by Actifeed A/B. The BC-P clone fed by Actifeed A/B showed a threefold cell volume increase and an increase of the specific consumption rate of glucose in the stationary phase. Since feeding was based on glucose this resulted in accumulation of amino acids for this feed, while this did not occur for the poorer feed (EFA/B). The same feed also led to an increase of cell size for the BC-G clone, but to a lesser extent.</description><identifier>ISSN: 0920-9069</identifier><identifier>EISSN: 1573-0778</identifier><identifier>DOI: 10.1007/s10616-016-0036-5</identifier><identifier>PMID: 27900626</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Amino acids ; Antibodies ; Batch culture ; Batch processes ; Biochemistry ; Biomedicine ; Biotechnology ; Cell culture ; Cell growth ; Cell size ; Chemistry ; Chemistry and Materials Science ; Cloning ; Fed-batch culture ; Feeding ; Glucose ; Lymphocytes B ; Media ; Metabolism ; Monoclonal antibodies ; Original ; Original Article ; Ovaries ; Phase transitions ; Productivity ; Proteins ; Stationary phase ; Suppliers</subject><ispartof>Cytotechnology (Dordrecht), 2017-02, Vol.69 (1), p.39-56</ispartof><rights>The Author(s) 2016</rights><rights>Copyright Springer Science & Business Media 2017</rights><rights>The Author(s) 2016. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c601t-e3ef4727f734cb5e51c30bca4d15b55532cf6c93afef7df2b6a259fbd86e5abf3</citedby><cites>FETCH-LOGICAL-c601t-e3ef4727f734cb5e51c30bca4d15b55532cf6c93afef7df2b6a259fbd86e5abf3</cites><orcidid>0000-0003-0561-846X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5264622/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2918252042?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,21387,27923,27924,33743,33744,41487,42556,43804,51318,53790,53792,64384,64386,64388,72240</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27900626$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pan, Xiao</creatorcontrib><creatorcontrib>Streefland, Mathieu</creatorcontrib><creatorcontrib>Dalm, Ciska</creatorcontrib><creatorcontrib>Wijffels, René H.</creatorcontrib><creatorcontrib>Martens, Dirk E.</creatorcontrib><title>Selection of chemically defined media for CHO cell fed-batch culture processes</title><title>Cytotechnology (Dordrecht)</title><addtitle>Cytotechnology</addtitle><addtitle>Cytotechnology</addtitle><description>Two CHO cell clones derived from the same parental CHO
BC®
cell line and producing the same monoclonal antibody (BC-G, a low producing clone; BC-P, a high producing clone) were tested in four basal media in all possible combinations with three feeds (=12 conditions) in fed-batch cultures. Higher amino acid feeding did not always lead to higher mAb production. The two clones showed differences in cell physiology, metabolism and optimal medium-feed combinations. During the phase transitions of all cultures, cell metabolism showed a shift represented by lower specific consumption and production rates, except for the specific glucose consumption rate in cultures fed by Actifeed A/B. The BC-P clone fed by Actifeed A/B showed a threefold cell volume increase and an increase of the specific consumption rate of glucose in the stationary phase. Since feeding was based on glucose this resulted in accumulation of amino acids for this feed, while this did not occur for the poorer feed (EFA/B). The same feed also led to an increase of cell size for the BC-G clone, but to a lesser extent.</description><subject>Amino acids</subject><subject>Antibodies</subject><subject>Batch culture</subject><subject>Batch processes</subject><subject>Biochemistry</subject><subject>Biomedicine</subject><subject>Biotechnology</subject><subject>Cell culture</subject><subject>Cell growth</subject><subject>Cell size</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Cloning</subject><subject>Fed-batch culture</subject><subject>Feeding</subject><subject>Glucose</subject><subject>Lymphocytes B</subject><subject>Media</subject><subject>Metabolism</subject><subject>Monoclonal antibodies</subject><subject>Original</subject><subject>Original Article</subject><subject>Ovaries</subject><subject>Phase transitions</subject><subject>Productivity</subject><subject>Proteins</subject><subject>Stationary phase</subject><subject>Suppliers</subject><issn>0920-9069</issn><issn>1573-0778</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kUFv1DAQhS1ERbeFH8AFWeLCJXQ8ie34goRWtEWq6AE4W44z7qbKxoudVOq_x6ttq4IEh9Ec5ps38_QYeyvgowDQZ1mAEqqCfUGtKvmCrYTUdQVaty_ZCgxCZUCZY3aS8y0AGC3qV-wYtQFQqFbs23cayc9DnHgM3G9oO3g3jve8pzBM1PMt9YPjISa-vrzmnsaRB-qrzs1-w_0yzksivkvRU86UX7Oj4MZMbx76Kft5_uXH-rK6ur74uv58VXkFYq6optBo1EHXje8kSeFr6LxreiE7KWWNPihvahco6D5gpxxKE7q-VSRdF-pT9umgu1u68qGnaU5utLs0bF26t9EN9s_JNGzsTbyzElWjEIvAhweBFH8tlGe7HfLenZsoLtmKtpHYtC2Ygr7_C72NS5qKPYtGtCgRGvwfJYqMNohtWyhxoHyKOScKTy8LsPtM7SFTC_sqmVpZdt499_q08RhiAfAA5DKabig9O_1P1d-0Yax5</recordid><startdate>20170201</startdate><enddate>20170201</enddate><creator>Pan, Xiao</creator><creator>Streefland, Mathieu</creator><creator>Dalm, Ciska</creator><creator>Wijffels, René H.</creator><creator>Martens, Dirk E.</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-0561-846X</orcidid></search><sort><creationdate>20170201</creationdate><title>Selection of chemically defined media for CHO cell fed-batch culture processes</title><author>Pan, Xiao ; Streefland, Mathieu ; Dalm, Ciska ; Wijffels, René H. ; Martens, Dirk E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c601t-e3ef4727f734cb5e51c30bca4d15b55532cf6c93afef7df2b6a259fbd86e5abf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Amino acids</topic><topic>Antibodies</topic><topic>Batch culture</topic><topic>Batch processes</topic><topic>Biochemistry</topic><topic>Biomedicine</topic><topic>Biotechnology</topic><topic>Cell culture</topic><topic>Cell growth</topic><topic>Cell size</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Cloning</topic><topic>Fed-batch culture</topic><topic>Feeding</topic><topic>Glucose</topic><topic>Lymphocytes B</topic><topic>Media</topic><topic>Metabolism</topic><topic>Monoclonal antibodies</topic><topic>Original</topic><topic>Original Article</topic><topic>Ovaries</topic><topic>Phase transitions</topic><topic>Productivity</topic><topic>Proteins</topic><topic>Stationary phase</topic><topic>Suppliers</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pan, Xiao</creatorcontrib><creatorcontrib>Streefland, Mathieu</creatorcontrib><creatorcontrib>Dalm, Ciska</creatorcontrib><creatorcontrib>Wijffels, René H.</creatorcontrib><creatorcontrib>Martens, Dirk E.</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cytotechnology (Dordrecht)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pan, Xiao</au><au>Streefland, Mathieu</au><au>Dalm, Ciska</au><au>Wijffels, René H.</au><au>Martens, Dirk E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Selection of chemically defined media for CHO cell fed-batch culture processes</atitle><jtitle>Cytotechnology (Dordrecht)</jtitle><stitle>Cytotechnology</stitle><addtitle>Cytotechnology</addtitle><date>2017-02-01</date><risdate>2017</risdate><volume>69</volume><issue>1</issue><spage>39</spage><epage>56</epage><pages>39-56</pages><issn>0920-9069</issn><eissn>1573-0778</eissn><abstract>Two CHO cell clones derived from the same parental CHO
BC®
cell line and producing the same monoclonal antibody (BC-G, a low producing clone; BC-P, a high producing clone) were tested in four basal media in all possible combinations with three feeds (=12 conditions) in fed-batch cultures. Higher amino acid feeding did not always lead to higher mAb production. The two clones showed differences in cell physiology, metabolism and optimal medium-feed combinations. During the phase transitions of all cultures, cell metabolism showed a shift represented by lower specific consumption and production rates, except for the specific glucose consumption rate in cultures fed by Actifeed A/B. The BC-P clone fed by Actifeed A/B showed a threefold cell volume increase and an increase of the specific consumption rate of glucose in the stationary phase. Since feeding was based on glucose this resulted in accumulation of amino acids for this feed, while this did not occur for the poorer feed (EFA/B). The same feed also led to an increase of cell size for the BC-G clone, but to a lesser extent.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>27900626</pmid><doi>10.1007/s10616-016-0036-5</doi><tpages>18</tpages><orcidid>https://orcid.org/0000-0003-0561-846X</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0920-9069 |
| ispartof | Cytotechnology (Dordrecht), 2017-02, Vol.69 (1), p.39-56 |
| issn | 0920-9069 1573-0778 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5264622 |
| source | SpringerNature Complete Journals; EZB-FREE-00999 freely available EZB journals; ProQuest Central UK/Ireland; PubMed Central; ProQuest Central |
| subjects | Amino acids Antibodies Batch culture Batch processes Biochemistry Biomedicine Biotechnology Cell culture Cell growth Cell size Chemistry Chemistry and Materials Science Cloning Fed-batch culture Feeding Glucose Lymphocytes B Media Metabolism Monoclonal antibodies Original Original Article Ovaries Phase transitions Productivity Proteins Stationary phase Suppliers |
| title | Selection of chemically defined media for CHO cell fed-batch culture processes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-10T10%3A20%3A21IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Selection%20of%20chemically%20defined%20media%20for%20CHO%20cell%20fed-batch%20culture%20processes&rft.jtitle=Cytotechnology%20(Dordrecht)&rft.au=Pan,%20Xiao&rft.date=2017-02-01&rft.volume=69&rft.issue=1&rft.spage=39&rft.epage=56&rft.pages=39-56&rft.issn=0920-9069&rft.eissn=1573-0778&rft_id=info:doi/10.1007/s10616-016-0036-5&rft_dat=%3Cproquest_pubme%3E1880792288%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2918252042&rft_id=info:pmid/27900626&rfr_iscdi=true |