Long-Term High-Resolution Imaging of Developing C. elegans Larvae with Microfluidics

Long-term studies of Caenorhabditis elegans larval development traditionally require tedious manual observations because larvae must move to develop, and existing immobilization techniques either perturb development or are unsuited for young larvae. Here, we present a simple microfluidic device to s...

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Veröffentlicht in:	Developmental cell 2017-01, Vol.40 (2), p.202-214
Hauptverfasser:	Keil, Wolfgang, Kutscher, Lena M., Shaham, Shai, Siggia, Eric D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Caenorhabditis elegans Caenorhabditis elegans - genetics Caenorhabditis elegans - growth & development Caenorhabditis elegans - metabolism Cell Cycle Cell Death Cell Division Cell Tracking Cell Transdifferentiation dendritic arborization Female Gene Expression Regulation, Developmental Imaging, Three-Dimensional Larva - metabolism larval development linker cell death long-term imaging Male microfluidics Microfluidics - methods Neurites - metabolism PDA neuron PVD neuron Time Factors Time-Lapse Imaging transdifferentiation Vulva - cytology Vulva - growth & development vulval development
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