In vitro formation of recycling vesicles from endosomes requires adaptor protein-1/clathrin and is regulated by rab4 and the connector rabaptin-5

The involvement of clathrin and associated adaptor proteins in receptor recycling from endosomes back to the plasma membrane is controversial. We have used an in vitro assay to identify the molecular requirements for the formation of recycling vesicles. Cells expressing the asialoglycoprotein recept...

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Veröffentlicht in:	Molecular biology of the cell 2004-11, Vol.15 (11), p.4990-5000
Hauptverfasser:	Pagano, Adriana, Crottet, Pascal, Prescianotto-Baschong, Cristina, Spiess, Martin
Format:	Artikel
Sprache:	eng
Schlagworte:	Adaptor Protein Complex 2 - metabolism Adaptor Protein Complex 3 - metabolism Animals Biotin - chemistry Biotinylation Cattle Cell Membrane - metabolism Cytosol - metabolism Endosomes - physiology Histones - metabolism Microscopy, Electron Models, Biological Protein Binding rab4 GTP-Binding Proteins - physiology Temperature Time Factors Transcription Factor AP-1 - metabolism Transcription Factor AP-1 - physiology Vesicular Transport Proteins - physiology
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