Profiling single-guide RNA specificity reveals a mismatch sensitive core sequence

Targeting specificity is an essential issue in the development of CRISPR-Cas technology. Using a luciferase activation assay, off-target cleavage activity of sgRNA was systematically investigated on single nucleotide-mismatched targets. In addition to confirming that PAM-proximal mismatches are less...

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Veröffentlicht in:	Scientific reports 2017-01, Vol.7 (1), p.40638-40638, Article 40638
Hauptverfasser:	Zheng, Ting, Hou, Yingzi, Zhang, Pingjing, Zhang, Zhenxi, Xu, Ying, Zhang, Letian, Niu, Leilei, Yang, Yi, Liang, Da, Yi, Fan, Peng, Wei, Feng, Wenjian, Yang, Ying, Chen, Jianxin, Zhu, York Yuanyuan, Zhang, Li-He, Du, Quan
Format:	Artikel
Sprache:	eng
Schlagworte:	42/41 631/337 631/61 Base Pairing Base Sequence CRISPR CRISPR-Cas Systems Endonuclease Gene Expression Profiling Genes, Reporter Genetic Engineering Genome Genomes Humanities and Social Sciences Humans multidisciplinary Mutation Nucleotide sequence Plasmids Ribonucleic acid RNA RNA, Guide, CRISPR-Cas Systems - chemistry RNA, Guide, CRISPR-Cas Systems - genetics Science Science (multidisciplinary)
Online-Zugang:	Volltext
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