Construction of Gpm6a/ReelinGFPCreERT2 by BAC recombination using a specific gene in hepatic mesothelial or stellate cells

AIM To prepare a Gpm6a/ReelinGFPCreERT2 construct with a rapid and reliable strategy using a bacterial artificial chromosome（BAC）. METHODS Gpm6 a and Reelin BACs were purified and transformed into SW102 E. coli by electroporation. The GFPCreE RT2 fragment was prepared from a shuttle vector and trans...

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Veröffentlicht in:	World journal of gastroenterology : WJG 2017-01, Vol.23 (2), p.224-231
Hauptverfasser:	Shi, Hong-Bo, Lou, Jin-Li, Shi, Hong-Lin, Ren, Feng, Chen, Yu, Duan, Zhong-Ping
Format:	Artikel
Sprache:	eng
Schlagworte:	Basic Study
Online-Zugang:	Volltext
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