Solid support quantitation of c-myc PCR products using a cleavable reporter
We described here a simple and specific methodology to quantify competitive PCR products for the determination of c-myc gene copies number. The c-myc oncogene is coamplified with a synthetic standard gene, followed by a separate hybridization of the two PCR products in conditions previously determin...
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| Veröffentlicht in: | Nucleic acids research 1994-02, Vol.22 (3), p.547-548 |
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| container_end_page | 548 |
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| container_issue | 3 |
| container_start_page | 547 |
| container_title | Nucleic acids research |
| container_volume | 22 |
| creator | Rhoer-Moja, Sandrine Bazin, Hervé Sauvaigo, Sylvie Chypre, Camille Vindimian, Monique |
| description | We described here a simple and specific methodology to quantify competitive PCR products for the determination of c-myc gene copies number. The c-myc oncogene is coamplified with a synthetic standard gene, followed by a separate hybridization of the two PCR products in conditions previously determined. The PCR efficiency, cycle number, sensitivity and hybridization parameters have been described, as well as the evaluation of the level of c-myc gene amplification through a reference curve established from serial normal DNA concentrations run along with the tumoral samples. |
| doi_str_mv | 10.1093/nar/22.3.547 |
| format | Article |
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The c-myc oncogene is coamplified with a synthetic standard gene, followed by a separate hybridization of the two PCR products in conditions previously determined. The PCR efficiency, cycle number, sensitivity and hybridization parameters have been described, as well as the evaluation of the level of c-myc gene amplification through a reference curve established from serial normal DNA concentrations run along with the tumoral samples.</description><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Diverse techniques</subject><subject>DNA Primers - chemistry</subject><subject>Fundamental and applied biological sciences. 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| fulltext | fulltext |
| identifier | ISSN: 0305-1048 |
| ispartof | Nucleic acids research, 1994-02, Vol.22 (3), p.547-548 |
| issn | 0305-1048 1362-4962 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_523623 |
| source | MEDLINE; Oxford University Press Journals Digital Archive Legacy; PubMed Central |
| subjects | Base Sequence Biological and medical sciences Diverse techniques DNA Primers - chemistry Fundamental and applied biological sciences. Psychology Gene Amplification Genes, myc Humans Molecular and cellular biology Molecular Sequence Data Polymerase Chain Reaction - methods |
| title | Solid support quantitation of c-myc PCR products using a cleavable reporter |
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