Solid support quantitation of c-myc PCR products using a cleavable reporter

We described here a simple and specific methodology to quantify competitive PCR products for the determination of c-myc gene copies number. The c-myc oncogene is coamplified with a synthetic standard gene, followed by a separate hybridization of the two PCR products in conditions previously determined. The PCR efficiency, cycle number, sensitivity and hybridization parameters have been described, as well as the evaluation of the level of c-myc gene amplification through a reference curve established from serial normal DNA concentrations run along with the tumoral samples.