LWR1 and LWR2 are required for osmoregulation and osmotic adjustment in Arabidopsis
With the goal of identifying molecular components of the low-water-potential response, we have carried out a two-part selection and screening strategy to identify new Arabidopsis mutants. Using a system of polyethylene glycol-infused agar plates to impose a constant low-water-potential stress, putat...
Gespeichert in:
| Veröffentlicht in: | Plant physiology (Bethesda) 2004-09, Vol.136 (1), p.2831-2842 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 2842 |
|---|---|
| container_issue | 1 |
| container_start_page | 2831 |
| container_title | Plant physiology (Bethesda) |
| container_volume | 136 |
| creator | Verslues, P.E Bray, E.A |
| description | With the goal of identifying molecular components of the low-water-potential response, we have carried out a two-part selection and screening strategy to identify new Arabidopsis mutants. Using a system of polyethylene glycol-infused agar plates to impose a constant low-water-potential stress, putative mutants impaired in low-water-potential induction of the tomato (Lycopersicon esculentum) le25 promoter were selected. These lines were then screened for altered accumulation of free Pro. The seedlings of 22 mutant lines had either higher or lower Pro content than wild type when exposed to low water potential. Two mutants, designated low-water-potential response1 (lwr1) and lwr2, were characterized in detail. In addition to higher Pro accumulation, lwr1 seedlings had higher total solute content, greater osmotic adjustment at low water potential, altered abscisic acid content, and increased sensitivity to applied abscisic acid with respect to Pro content. lwr1 also had altered growth and morphology. lwr2, in contrast, had lower Pro content and less osmotic adjustment leading to greater water loss at low water potential. Both lwr1 and lwr2 also had altered leaf solute content and water relations in unstressed soil-grown plants. In both mutants, the effects on solute content were too large to be explained by the changes in Pro content alone, indicating that LWR1 and LWR2 affect multiple aspects of cellular osmoregulation. |
| doi_str_mv | 10.1104/pp.104.045856 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_523345</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>4356633</jstor_id><sourcerecordid>4356633</sourcerecordid><originalsourceid>FETCH-LOGICAL-c457t-ec6626f5d11205c6295d214cf4a6a09dd39da01c7937b1f1e75cdb5b9b7f4ef73</originalsourceid><addsrcrecordid>eNpVkUtv1DAUhS0EotPCkh0Cb8oug992Fl1UFS9pJCRKxdJy_Bg8SuLUTpD493jIqIXVufL97r1HPgC8wmiLMWLvp2lbZYsYV1w8ARvMKWkIZ-op2CBUa6RUewbOSzkghDDF7Dk4qxCTUqkNuN39-IahGR2sBYEme5j9_RKzdzCkDFMZUvb7pTdzTONf8Pg0RwuNOyxlHvw4wzjC62y66NJUYnkBngXTF__ypBfg7uOH7zefm93XT19urneNZVzOjbdCEBG4w5ggbgVpuSOY2cCMMKh1jrbOIGxlS2WHA_aSW9fxru1kYD5IegGu1r3T0g3e2eokm15POQ4m_9bJRP1_Z4w_9T790pxQynidf3eaz-l-8WXWQyzW970ZfVqKFkIpKSWtYLOCNqdSsg8PNzDSxxT0NOmjrClU_s2_xh7p07dX4PIEmGJNH7IZbSyPnMCEMoEq93rlDmVO-aHPKBeCHn29XdvBJG32ua64uyU1ZIRarghp6R-K1qOQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>66887773</pqid></control><display><type>article</type><title>LWR1 and LWR2 are required for osmoregulation and osmotic adjustment in Arabidopsis</title><source>Jstor Complete Legacy</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Verslues, P.E ; Bray, E.A</creator><creatorcontrib>Verslues, P.E ; Bray, E.A</creatorcontrib><description>With the goal of identifying molecular components of the low-water-potential response, we have carried out a two-part selection and screening strategy to identify new Arabidopsis mutants. Using a system of polyethylene glycol-infused agar plates to impose a constant low-water-potential stress, putative mutants impaired in low-water-potential induction of the tomato (Lycopersicon esculentum) le25 promoter were selected. These lines were then screened for altered accumulation of free Pro. The seedlings of 22 mutant lines had either higher or lower Pro content than wild type when exposed to low water potential. Two mutants, designated low-water-potential response1 (lwr1) and lwr2, were characterized in detail. In addition to higher Pro accumulation, lwr1 seedlings had higher total solute content, greater osmotic adjustment at low water potential, altered abscisic acid content, and increased sensitivity to applied abscisic acid with respect to Pro content. lwr1 also had altered growth and morphology. lwr2, in contrast, had lower Pro content and less osmotic adjustment leading to greater water loss at low water potential. Both lwr1 and lwr2 also had altered leaf solute content and water relations in unstressed soil-grown plants. In both mutants, the effects on solute content were too large to be explained by the changes in Pro content alone, indicating that LWR1 and LWR2 affect multiple aspects of cellular osmoregulation.</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.104.045856</identifier><identifier>PMID: 15347788</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Biologists</publisher><subject>abscisic acid ; Abscisic Acid - metabolism ; Agronomy. Soil science and plant productions ; Alcohols ; Arabidopsis - genetics ; Arabidopsis - growth & development ; Arabidopsis - metabolism ; Arabidopsis thaliana ; Biological and medical sciences ; Economic plant physiology ; Environmental Stress and Adaptation ; Fundamental and applied biological sciences. Psychology ; Genes, Plant ; Genes. Genome ; Growth regulators ; Leaves ; Metabolism ; Molecular and cellular biology ; Molecular genetics ; Mutation ; Osmoregulation ; osmosis ; Osmotic Pressure ; Phenotype ; phenotypic variation ; Plant cells ; plant growth ; Plant Leaves - metabolism ; Plant morphology ; Plant physiology and development ; plant proteins ; plant response ; plant stress ; Plants ; Plants, Genetically Modified ; Potassium - metabolism ; proline ; Proline - metabolism ; promoter regions ; Seedlings ; soil water potential ; Solanum lycopersicum var. lycopersicum ; Solutes ; tomatoes ; Turgor pressure ; Water and solutes. Absorption, translocation and permeability ; Water loss ; Water relations, transpiration, stomata ; Water-Electrolyte Balance - genetics</subject><ispartof>Plant physiology (Bethesda), 2004-09, Vol.136 (1), p.2831-2842</ispartof><rights>Copyright 2004 American Society of Plant Biologists</rights><rights>2004 INIST-CNRS</rights><rights>Copyright © 2004, American Society of Plant Biologists 2004</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c457t-ec6626f5d11205c6295d214cf4a6a09dd39da01c7937b1f1e75cdb5b9b7f4ef73</citedby><cites>FETCH-LOGICAL-c457t-ec6626f5d11205c6295d214cf4a6a09dd39da01c7937b1f1e75cdb5b9b7f4ef73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4356633$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4356633$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,776,780,799,881,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16123460$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15347788$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Verslues, P.E</creatorcontrib><creatorcontrib>Bray, E.A</creatorcontrib><title>LWR1 and LWR2 are required for osmoregulation and osmotic adjustment in Arabidopsis</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>With the goal of identifying molecular components of the low-water-potential response, we have carried out a two-part selection and screening strategy to identify new Arabidopsis mutants. Using a system of polyethylene glycol-infused agar plates to impose a constant low-water-potential stress, putative mutants impaired in low-water-potential induction of the tomato (Lycopersicon esculentum) le25 promoter were selected. These lines were then screened for altered accumulation of free Pro. The seedlings of 22 mutant lines had either higher or lower Pro content than wild type when exposed to low water potential. Two mutants, designated low-water-potential response1 (lwr1) and lwr2, were characterized in detail. In addition to higher Pro accumulation, lwr1 seedlings had higher total solute content, greater osmotic adjustment at low water potential, altered abscisic acid content, and increased sensitivity to applied abscisic acid with respect to Pro content. lwr1 also had altered growth and morphology. lwr2, in contrast, had lower Pro content and less osmotic adjustment leading to greater water loss at low water potential. Both lwr1 and lwr2 also had altered leaf solute content and water relations in unstressed soil-grown plants. In both mutants, the effects on solute content were too large to be explained by the changes in Pro content alone, indicating that LWR1 and LWR2 affect multiple aspects of cellular osmoregulation.</description><subject>abscisic acid</subject><subject>Abscisic Acid - metabolism</subject><subject>Agronomy. Soil science and plant productions</subject><subject>Alcohols</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - growth & development</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis thaliana</subject><subject>Biological and medical sciences</subject><subject>Economic plant physiology</subject><subject>Environmental Stress and Adaptation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Plant</subject><subject>Genes. Genome</subject><subject>Growth regulators</subject><subject>Leaves</subject><subject>Metabolism</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Mutation</subject><subject>Osmoregulation</subject><subject>osmosis</subject><subject>Osmotic Pressure</subject><subject>Phenotype</subject><subject>phenotypic variation</subject><subject>Plant cells</subject><subject>plant growth</subject><subject>Plant Leaves - metabolism</subject><subject>Plant morphology</subject><subject>Plant physiology and development</subject><subject>plant proteins</subject><subject>plant response</subject><subject>plant stress</subject><subject>Plants</subject><subject>Plants, Genetically Modified</subject><subject>Potassium - metabolism</subject><subject>proline</subject><subject>Proline - metabolism</subject><subject>promoter regions</subject><subject>Seedlings</subject><subject>soil water potential</subject><subject>Solanum lycopersicum var. lycopersicum</subject><subject>Solutes</subject><subject>tomatoes</subject><subject>Turgor pressure</subject><subject>Water and solutes. Absorption, translocation and permeability</subject><subject>Water loss</subject><subject>Water relations, transpiration, stomata</subject><subject>Water-Electrolyte Balance - genetics</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkUtv1DAUhS0EotPCkh0Cb8oug992Fl1UFS9pJCRKxdJy_Bg8SuLUTpD493jIqIXVufL97r1HPgC8wmiLMWLvp2lbZYsYV1w8ARvMKWkIZ-op2CBUa6RUewbOSzkghDDF7Dk4qxCTUqkNuN39-IahGR2sBYEme5j9_RKzdzCkDFMZUvb7pTdzTONf8Pg0RwuNOyxlHvw4wzjC62y66NJUYnkBngXTF__ypBfg7uOH7zefm93XT19urneNZVzOjbdCEBG4w5ggbgVpuSOY2cCMMKh1jrbOIGxlS2WHA_aSW9fxru1kYD5IegGu1r3T0g3e2eokm15POQ4m_9bJRP1_Z4w_9T790pxQynidf3eaz-l-8WXWQyzW970ZfVqKFkIpKSWtYLOCNqdSsg8PNzDSxxT0NOmjrClU_s2_xh7p07dX4PIEmGJNH7IZbSyPnMCEMoEq93rlDmVO-aHPKBeCHn29XdvBJG32ua64uyU1ZIRarghp6R-K1qOQ</recordid><startdate>20040901</startdate><enddate>20040901</enddate><creator>Verslues, P.E</creator><creator>Bray, E.A</creator><general>American Society of Plant Biologists</general><general>American Society of Plant Physiologists</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20040901</creationdate><title>LWR1 and LWR2 are required for osmoregulation and osmotic adjustment in Arabidopsis</title><author>Verslues, P.E ; Bray, E.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c457t-ec6626f5d11205c6295d214cf4a6a09dd39da01c7937b1f1e75cdb5b9b7f4ef73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>abscisic acid</topic><topic>Abscisic Acid - metabolism</topic><topic>Agronomy. Soil science and plant productions</topic><topic>Alcohols</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - growth & development</topic><topic>Arabidopsis - metabolism</topic><topic>Arabidopsis thaliana</topic><topic>Biological and medical sciences</topic><topic>Economic plant physiology</topic><topic>Environmental Stress and Adaptation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Plant</topic><topic>Genes. Genome</topic><topic>Growth regulators</topic><topic>Leaves</topic><topic>Metabolism</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Mutation</topic><topic>Osmoregulation</topic><topic>osmosis</topic><topic>Osmotic Pressure</topic><topic>Phenotype</topic><topic>phenotypic variation</topic><topic>Plant cells</topic><topic>plant growth</topic><topic>Plant Leaves - metabolism</topic><topic>Plant morphology</topic><topic>Plant physiology and development</topic><topic>plant proteins</topic><topic>plant response</topic><topic>plant stress</topic><topic>Plants</topic><topic>Plants, Genetically Modified</topic><topic>Potassium - metabolism</topic><topic>proline</topic><topic>Proline - metabolism</topic><topic>promoter regions</topic><topic>Seedlings</topic><topic>soil water potential</topic><topic>Solanum lycopersicum var. lycopersicum</topic><topic>Solutes</topic><topic>tomatoes</topic><topic>Turgor pressure</topic><topic>Water and solutes. Absorption, translocation and permeability</topic><topic>Water loss</topic><topic>Water relations, transpiration, stomata</topic><topic>Water-Electrolyte Balance - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Verslues, P.E</creatorcontrib><creatorcontrib>Bray, E.A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Verslues, P.E</au><au>Bray, E.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>LWR1 and LWR2 are required for osmoregulation and osmotic adjustment in Arabidopsis</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>2004-09-01</date><risdate>2004</risdate><volume>136</volume><issue>1</issue><spage>2831</spage><epage>2842</epage><pages>2831-2842</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>With the goal of identifying molecular components of the low-water-potential response, we have carried out a two-part selection and screening strategy to identify new Arabidopsis mutants. Using a system of polyethylene glycol-infused agar plates to impose a constant low-water-potential stress, putative mutants impaired in low-water-potential induction of the tomato (Lycopersicon esculentum) le25 promoter were selected. These lines were then screened for altered accumulation of free Pro. The seedlings of 22 mutant lines had either higher or lower Pro content than wild type when exposed to low water potential. Two mutants, designated low-water-potential response1 (lwr1) and lwr2, were characterized in detail. In addition to higher Pro accumulation, lwr1 seedlings had higher total solute content, greater osmotic adjustment at low water potential, altered abscisic acid content, and increased sensitivity to applied abscisic acid with respect to Pro content. lwr1 also had altered growth and morphology. lwr2, in contrast, had lower Pro content and less osmotic adjustment leading to greater water loss at low water potential. Both lwr1 and lwr2 also had altered leaf solute content and water relations in unstressed soil-grown plants. In both mutants, the effects on solute content were too large to be explained by the changes in Pro content alone, indicating that LWR1 and LWR2 affect multiple aspects of cellular osmoregulation.</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Biologists</pub><pmid>15347788</pmid><doi>10.1104/pp.104.045856</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0032-0889 |
| ispartof | Plant physiology (Bethesda), 2004-09, Vol.136 (1), p.2831-2842 |
| issn | 0032-0889 1532-2548 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_523345 |
| source | Jstor Complete Legacy; Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | abscisic acid Abscisic Acid - metabolism Agronomy. Soil science and plant productions Alcohols Arabidopsis - genetics Arabidopsis - growth & development Arabidopsis - metabolism Arabidopsis thaliana Biological and medical sciences Economic plant physiology Environmental Stress and Adaptation Fundamental and applied biological sciences. Psychology Genes, Plant Genes. Genome Growth regulators Leaves Metabolism Molecular and cellular biology Molecular genetics Mutation Osmoregulation osmosis Osmotic Pressure Phenotype phenotypic variation Plant cells plant growth Plant Leaves - metabolism Plant morphology Plant physiology and development plant proteins plant response plant stress Plants Plants, Genetically Modified Potassium - metabolism proline Proline - metabolism promoter regions Seedlings soil water potential Solanum lycopersicum var. lycopersicum Solutes tomatoes Turgor pressure Water and solutes. Absorption, translocation and permeability Water loss Water relations, transpiration, stomata Water-Electrolyte Balance - genetics |
| title | LWR1 and LWR2 are required for osmoregulation and osmotic adjustment in Arabidopsis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-30T16%3A12%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=LWR1%20and%20LWR2%20are%20required%20for%20osmoregulation%20and%20osmotic%20adjustment%20in%20Arabidopsis&rft.jtitle=Plant%20physiology%20(Bethesda)&rft.au=Verslues,%20P.E&rft.date=2004-09-01&rft.volume=136&rft.issue=1&rft.spage=2831&rft.epage=2842&rft.pages=2831-2842&rft.issn=0032-0889&rft.eissn=1532-2548&rft.coden=PPHYA5&rft_id=info:doi/10.1104/pp.104.045856&rft_dat=%3Cjstor_pubme%3E4356633%3C/jstor_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=66887773&rft_id=info:pmid/15347788&rft_jstor_id=4356633&rfr_iscdi=true |