Cell-Specific Expression of mRNAs Encoding Na+, K+-ATPase α- and β-Subunit Isoforms within the Rat Central Nervous System
We have used in situ hybridization histochemistry to analyze the subcellular distribution of mRNAs encoding Na,K-ATPase α- and β-subunit isoforms in the rat central nervous system. Substantial differences in the cell-specific pattern of expression were found for the genes encoding three isoforms of...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1991-08, Vol.88 (16), p.7425-7429 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Watts, Alan G. Sanchez-Watts, Graciela Emanuel, Janet Rettig Levenson, Robert |
| description | We have used in situ hybridization histochemistry to analyze the subcellular distribution of mRNAs encoding Na,K-ATPase α- and β-subunit isoforms in the rat central nervous system. Substantial differences in the cell-specific pattern of expression were found for the genes encoding three isoforms of the α subunit. Transcripts of α1-subunit gene were detected in virtually all cell types and structures examined. Expression of α2-subunit mRNA was characteristic of glia, whereas α3-subunit transcripts were predominant in neurons. Transcripts encoding the β1 subunit were detected in neurons, whereas β2-subunit mRNA expression was characteristic of glia. mRNA encoding both β-subunit isoforms was present in choroidal epithelial cells. The distribution pattern of α- and β-subunit mRNAs in structures throughout the central nervous system is consistent with the possibility of six structurally distinct Na+,K+-ATPase isoenzymes. |
| doi_str_mv | 10.1073/pnas.88.16.7425 |
| format | Article |
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Substantial differences in the cell-specific pattern of expression were found for the genes encoding three isoforms of the α subunit. Transcripts of α1-subunit gene were detected in virtually all cell types and structures examined. Expression of α2-subunit mRNA was characteristic of glia, whereas α3-subunit transcripts were predominant in neurons. Transcripts encoding the β1 subunit were detected in neurons, whereas β2-subunit mRNA expression was characteristic of glia. mRNA encoding both β-subunit isoforms was present in choroidal epithelial cells. The distribution pattern of α- and β-subunit mRNAs in structures throughout the central nervous system is consistent with the possibility of six structurally distinct Na+,K+-ATPase isoenzymes.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.88.16.7425</identifier><identifier>PMID: 1651505</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Animals ; Arachnoid ; Biological and medical sciences ; Brain - enzymology ; Central nervous system ; Central Nervous System - cytology ; Central Nervous System - enzymology ; Choroid Plexus - enzymology ; Complementary DNA ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Isoenzymes - genetics ; Macromolecular Substances ; Male ; Messenger RNA ; Molecular and cellular biology ; Molecular genetics ; Neuroglia ; Neurons ; Nucleic Acid Hybridization ; Organ Specificity ; Photoreceptors ; Pineal gland ; Protein isoforms ; Purkinje cells ; Rats ; Rats, Inbred Strains ; RNA Probes ; RNA, Messenger - analysis ; RNA, Messenger - genetics ; Sodium-Potassium-Exchanging ATPase - genetics ; Spinal Cord - enzymology ; Transcription, Genetic</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1991-08, Vol.88 (16), p.7425-7429</ispartof><rights>Copyright 1991 The National Academy of Sciences of the United States of America</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4725-3aad2a609f31bd96eef5a617b46401ea271fa2227113788da6d9dc91ab7207523</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/88/16.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2357696$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2357696$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4980625$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1651505$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Watts, Alan G.</creatorcontrib><creatorcontrib>Sanchez-Watts, Graciela</creatorcontrib><creatorcontrib>Emanuel, Janet Rettig</creatorcontrib><creatorcontrib>Levenson, Robert</creatorcontrib><title>Cell-Specific Expression of mRNAs Encoding Na+, K+-ATPase α- and β-Subunit Isoforms within the Rat Central Nervous System</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>We have used in situ hybridization histochemistry to analyze the subcellular distribution of mRNAs encoding Na,K-ATPase α- and β-subunit isoforms in the rat central nervous system. Substantial differences in the cell-specific pattern of expression were found for the genes encoding three isoforms of the α subunit. Transcripts of α1-subunit gene were detected in virtually all cell types and structures examined. Expression of α2-subunit mRNA was characteristic of glia, whereas α3-subunit transcripts were predominant in neurons. Transcripts encoding the β1 subunit were detected in neurons, whereas β2-subunit mRNA expression was characteristic of glia. mRNA encoding both β-subunit isoforms was present in choroidal epithelial cells. The distribution pattern of α- and β-subunit mRNAs in structures throughout the central nervous system is consistent with the possibility of six structurally distinct Na+,K+-ATPase isoenzymes.</description><subject>Animals</subject><subject>Arachnoid</subject><subject>Biological and medical sciences</subject><subject>Brain - enzymology</subject><subject>Central nervous system</subject><subject>Central Nervous System - cytology</subject><subject>Central Nervous System - enzymology</subject><subject>Choroid Plexus - enzymology</subject><subject>Complementary DNA</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Isoenzymes - genetics</subject><subject>Macromolecular Substances</subject><subject>Male</subject><subject>Messenger RNA</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Neuroglia</subject><subject>Neurons</subject><subject>Nucleic Acid Hybridization</subject><subject>Organ Specificity</subject><subject>Photoreceptors</subject><subject>Pineal gland</subject><subject>Protein isoforms</subject><subject>Purkinje cells</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>RNA Probes</subject><subject>RNA, Messenger - analysis</subject><subject>RNA, Messenger - genetics</subject><subject>Sodium-Potassium-Exchanging ATPase - genetics</subject><subject>Spinal Cord - enzymology</subject><subject>Transcription, Genetic</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcFu1DAURSMEKkNhzQaQFwgWJVPbSexEYjMaDVBRDahT1taLY3dcJXawndKKr4IP6TeRYYaBbli9xT33vmffJHlK8JRgnh33FsK0LKeETXlOi3vJhOCKpCyv8P1kgjHlaZnT_GHyKIRLjHFVlPggOSCsIAUuJsn3uWrbdNUrabSRaHHdexWCcRY5jbqz5SyghZWuMfYCLeHoDfp4lM7OP0NQ6PZHisA26PZnuhrqwZqIToLTzncBfTNxbSyKa4XOIKK5stFDi5bKX7khoNVNiKp7nDzQ0Ab1ZDcPky_vFufzD-npp_cn89lpKnNOizQDaCgwXOmM1E3FlNIFMMLrnOWYKKCcaKB0HCTjZdkAa6pGVgRqTjEvaHaYvN3m9kPdqUZujxG9Nx34G-HAiLuKNWtx4a7E6MXlaH-1s3v3dVAhis4EOX4bWDU-RoxbMkZzNoLHW1B6F4JXer-CYLFpS2zaEmUpCBObtkbH838v-8tv6xn1lzsdgoRWe7DShD2WVyVmv2Ne7LBN_h_1zp7X_wWEHto2qus4ks-25GWIzu9RmhWcVSz7BW8hwF8</recordid><startdate>19910815</startdate><enddate>19910815</enddate><creator>Watts, Alan G.</creator><creator>Sanchez-Watts, Graciela</creator><creator>Emanuel, Janet Rettig</creator><creator>Levenson, Robert</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19910815</creationdate><title>Cell-Specific Expression of mRNAs Encoding Na+, K+-ATPase α- and β-Subunit Isoforms within the Rat Central Nervous System</title><author>Watts, Alan G. ; Sanchez-Watts, Graciela ; Emanuel, Janet Rettig ; Levenson, Robert</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4725-3aad2a609f31bd96eef5a617b46401ea271fa2227113788da6d9dc91ab7207523</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Animals</topic><topic>Arachnoid</topic><topic>Biological and medical sciences</topic><topic>Brain - enzymology</topic><topic>Central nervous system</topic><topic>Central Nervous System - cytology</topic><topic>Central Nervous System - enzymology</topic><topic>Choroid Plexus - enzymology</topic><topic>Complementary DNA</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Isoenzymes - genetics</topic><topic>Macromolecular Substances</topic><topic>Male</topic><topic>Messenger RNA</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Neuroglia</topic><topic>Neurons</topic><topic>Nucleic Acid Hybridization</topic><topic>Organ Specificity</topic><topic>Photoreceptors</topic><topic>Pineal gland</topic><topic>Protein isoforms</topic><topic>Purkinje cells</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>RNA Probes</topic><topic>RNA, Messenger - analysis</topic><topic>RNA, Messenger - genetics</topic><topic>Sodium-Potassium-Exchanging ATPase - genetics</topic><topic>Spinal Cord - enzymology</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Watts, Alan G.</creatorcontrib><creatorcontrib>Sanchez-Watts, Graciela</creatorcontrib><creatorcontrib>Emanuel, Janet Rettig</creatorcontrib><creatorcontrib>Levenson, Robert</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Watts, Alan G.</au><au>Sanchez-Watts, Graciela</au><au>Emanuel, Janet Rettig</au><au>Levenson, Robert</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cell-Specific Expression of mRNAs Encoding Na+, K+-ATPase α- and β-Subunit Isoforms within the Rat Central Nervous System</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1991-08-15</date><risdate>1991</risdate><volume>88</volume><issue>16</issue><spage>7425</spage><epage>7429</epage><pages>7425-7429</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>We have used in situ hybridization histochemistry to analyze the subcellular distribution of mRNAs encoding Na,K-ATPase α- and β-subunit isoforms in the rat central nervous system. Substantial differences in the cell-specific pattern of expression were found for the genes encoding three isoforms of the α subunit. Transcripts of α1-subunit gene were detected in virtually all cell types and structures examined. Expression of α2-subunit mRNA was characteristic of glia, whereas α3-subunit transcripts were predominant in neurons. Transcripts encoding the β1 subunit were detected in neurons, whereas β2-subunit mRNA expression was characteristic of glia. mRNA encoding both β-subunit isoforms was present in choroidal epithelial cells. The distribution pattern of α- and β-subunit mRNAs in structures throughout the central nervous system is consistent with the possibility of six structurally distinct Na+,K+-ATPase isoenzymes.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>1651505</pmid><doi>10.1073/pnas.88.16.7425</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1991-08, Vol.88 (16), p.7425-7429 |
| issn | 0027-8424 1091-6490 |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Animals Arachnoid Biological and medical sciences Brain - enzymology Central nervous system Central Nervous System - cytology Central Nervous System - enzymology Choroid Plexus - enzymology Complementary DNA Fundamental and applied biological sciences. Psychology Gene Expression Isoenzymes - genetics Macromolecular Substances Male Messenger RNA Molecular and cellular biology Molecular genetics Neuroglia Neurons Nucleic Acid Hybridization Organ Specificity Photoreceptors Pineal gland Protein isoforms Purkinje cells Rats Rats, Inbred Strains RNA Probes RNA, Messenger - analysis RNA, Messenger - genetics Sodium-Potassium-Exchanging ATPase - genetics Spinal Cord - enzymology Transcription, Genetic |
| title | Cell-Specific Expression of mRNAs Encoding Na+, K+-ATPase α- and β-Subunit Isoforms within the Rat Central Nervous System |
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