A Cas9-based toolkit to program gene expression in Saccharomyces cerevisiae

Despite the extensive use of Saccharomyces cerevisiae as a platform for synthetic biology, strain engineering remains slow and laborious. Here, we employ CRISPR/Cas9 technology to build a cloning-free toolkit that addresses commonly encountered obstacles in metabolic engineering, including chromosom...

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Veröffentlicht in:	Nucleic acids research 2017-01, Vol.45 (1), p.496-508
Hauptverfasser:	Reider Apel, Amanda, d'Espaux, Leo, Wehrs, Maren, Sachs, Daniel, Li, Rachel A, Tong, Gary J, Garber, Megan, Nnadi, Oge, Zhuang, William, Hillson, Nathan J, Keasling, Jay D, Mukhopadhyay, Aindrila
Format:	Artikel
Sprache:	eng
Schlagworte:	60 APPLIED LIFE SCIENCES Bacterial Proteins - genetics Bacterial Proteins - metabolism BASIC BIOLOGICAL SCIENCES catabolism chromosomes cloning Clustered Regularly Interspaced Short Palindromic Repeats crispr CRISPR-Associated Protein 9 CRISPR-Cas Systems DNA DNA fragments DNA, Fungal - genetics DNA, Fungal - metabolism donors Endonucleases - genetics Endonucleases - metabolism engineering enzymes fluorescence gene editing Gene Expression Genetic Engineering - methods genetics internet intracellular protein transport Isomerases - genetics Isomerases - metabolism oncogenes plasmids Plasmids - chemistry Plasmids - metabolism Promoter Regions, Genetic RNA, Guide, CRISPR-Cas Systems - genetics RNA, Guide, CRISPR-Cas Systems - metabolism saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Software solubility Synthetic Biology and Bioengineering yeasts
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