Development and application of a selective PCR-denaturing gradient gel electrophoresis approach to detect a recently cultivated Bacillus group predominant in soil

The worldwide presence of a hitherto-nondescribed group of predominant soil microorganisms related to Bacillus benzoevorans was analyzed after development of two sets of selective primers targeting 16S rRNA genes in combination with denaturing gradient gel electrophoresis (DGGE). The high abundance...

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Veröffentlicht in:	Applied and Environmental Microbiology 2004-10, Vol.70 (10), p.5801-5809
Hauptverfasser:	Tzeneva, V.A, Li, Y, Felske, A.D.M, Vos, W.M. de, Akkermans, A.D.L, Vaughan, E.E, Smidt, H
Format:	Artikel
Sprache:	eng
Schlagworte:	16s ribosomal-rna Bacillus - classification Bacillus - genetics Bacillus - isolation & purification Bacillus benzoevorans Bacteria Bacteriological methods and techniques used in bacteriology Bacteriology Base Sequence Biological and medical sciences denaturing gradient gel electrophoresis dna DNA Fingerprinting - methods DNA Primers - genetics DNA, Bacterial - genetics DNA, Bacterial - isolation & purification drentse Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology gel electrophoresis Genes Genes, Bacterial genetic variation geographical variation grassland soils identification in-situ detection microbial detection Microbial Ecology Microbiology molecular microbial diversity molecular sequence data netherlands Nucleic Acid Denaturation nucleotide sequences Phylogeny polymerase chain reaction Polymerase Chain Reaction - methods Ribonucleic acid ribosomal RNA RNA RNA, Bacterial - genetics RNA, Ribosomal, 16S - genetics soil bacteria Soil Microbiology Soil microorganisms sp nov Species Specificity
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