Detection of West Nile virus in six mosquito species in synchrony with seroconversion among sentinel chickens in India
West Nile virus (WNV) is a zoonotic flavivirus maintained in mosquito-bird transmission cycle. Although humans are accidental hosts, fatal outcomes following WNV infection have been reported from India. Studies have identified WNV as an important etiological agent causing acute encephalitis syndrome...
Gespeichert in:
| Veröffentlicht in: | Parasites & vectors 2017-01, Vol.10 (1), p.13-13, Article 13 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 13 |
|---|---|
| container_issue | 1 |
| container_start_page | 13 |
| container_title | Parasites & vectors |
| container_volume | 10 |
| creator | Khan, Siraj A Chowdhury, Purvita Choudhury, Parveena Dutta, Prafulla |
| description | West Nile virus (WNV) is a zoonotic flavivirus maintained in mosquito-bird transmission cycle. Although humans are accidental hosts, fatal outcomes following WNV infection have been reported from India. Studies have identified WNV as an important etiological agent causing acute encephalitis syndrome in Assam, Northeast India. While circulation of WNV is evident, the role of vectors and avian hosts involved in the transmission remains unclear. In this study we identified local mosquito species for evidence of WNV infection along with seroconversion among sentinel chickens.
Mosquitoes were collected and pooled species wise from June 2014 through December 2015. Virus was screened using reverse transcriptase PCR followed by sequencing and phylogenetic analysis. Sentinel chicken blood was screened for WNV antibody to assess their role in WNV transmission.
A total of 52,882 mosquitoes belonging to 16 species were collected. WNV was detected in 18 pools of Culex vishnui, Culex tritaeniorhynchus, Culex quinquefasciatus, Culex whitmorei, Culex pseudovishnui and Mansonia uniformis. Phylogenetic analysis revealed that all mosquito derived sequences belonged to Lineage 5 and were 99-100% similar to the Assam strain of WNV isolated from human CSF sample in 2007. All sentinel chickens had seroconverted by the month of July that happens to be the peak WNV transmission month among humans as well.
To the best of our knowledge, this is the first report of WNV identification from field-collected Cx. pseudovishnui and Mansonia uniformis in India. Our study demonstrates potential vectors which may play a crucial role in WNV transmission and should be considered in the vector control strategies. Additionally, our study highlights the role of sentinel chickens for WNV surveillance. |
| doi_str_mv | 10.1186/s13071-016-1948-9 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5219652</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A476741005</galeid><sourcerecordid>A476741005</sourcerecordid><originalsourceid>FETCH-LOGICAL-c528t-dbd8f934e4317e298e7187a84407c75c0484f0b8cc3660ecdabbc4397cc96d833</originalsourceid><addsrcrecordid>eNptkktv1DAUhSMEoqXwA9ggS2zaRYodP2JvkKpSYKQKJB5iaTnOzYxLYk_tZOj8exymVDMIeWHr-jvH9vUpipcEnxMixZtEKK5JiYkoiWKyVI-KY1JzUVKK-eO99VHxLKUbjAVWXDwtjiqJBVGYHhebdzCCHV3wKHToB6QRfXI9oI2LU0LOo-Tu0BDS7eTGgNIarINdfevtKga_Rb_cuEIJYrDBbyCm2csMwS9z0Y_OQ4_sytmf4P8IF7515nnxpDN9ghf380nx_f3Vt8uP5fXnD4vLi-vS8kqOZdu0slOUAaOkhkpJqImsjWQM17bmFjPJOtxIa6kQGGxrmsYyqmprlWglpSfF253vemoGaG2-UDS9Xkc3mLjVwTh9uOPdSi_DRvOKKMGrbHB6bxDD7ZTboweXLPS98RCmpInkgivO1Iy-_ge9CVP0-XmZEpwyKas9aml60M53IZ9rZ1N9wWpRM4Ixz9T5f6g8Whhc7jN0-ZMOBWcHgsyMcDcuzZSSXnz9csiSHWtjSClC99APgvUcLL0Lls7B0nOwtMqaV_uNfFD8TRL9DS8vyQw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1865348822</pqid></control><display><type>article</type><title>Detection of West Nile virus in six mosquito species in synchrony with seroconversion among sentinel chickens in India</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>SpringerNature Journals</source><source>PubMed Central</source><source>Springer Nature OA/Free Journals</source><creator>Khan, Siraj A ; Chowdhury, Purvita ; Choudhury, Parveena ; Dutta, Prafulla</creator><creatorcontrib>Khan, Siraj A ; Chowdhury, Purvita ; Choudhury, Parveena ; Dutta, Prafulla</creatorcontrib><description>West Nile virus (WNV) is a zoonotic flavivirus maintained in mosquito-bird transmission cycle. Although humans are accidental hosts, fatal outcomes following WNV infection have been reported from India. Studies have identified WNV as an important etiological agent causing acute encephalitis syndrome in Assam, Northeast India. While circulation of WNV is evident, the role of vectors and avian hosts involved in the transmission remains unclear. In this study we identified local mosquito species for evidence of WNV infection along with seroconversion among sentinel chickens.
Mosquitoes were collected and pooled species wise from June 2014 through December 2015. Virus was screened using reverse transcriptase PCR followed by sequencing and phylogenetic analysis. Sentinel chicken blood was screened for WNV antibody to assess their role in WNV transmission.
A total of 52,882 mosquitoes belonging to 16 species were collected. WNV was detected in 18 pools of Culex vishnui, Culex tritaeniorhynchus, Culex quinquefasciatus, Culex whitmorei, Culex pseudovishnui and Mansonia uniformis. Phylogenetic analysis revealed that all mosquito derived sequences belonged to Lineage 5 and were 99-100% similar to the Assam strain of WNV isolated from human CSF sample in 2007. All sentinel chickens had seroconverted by the month of July that happens to be the peak WNV transmission month among humans as well.
To the best of our knowledge, this is the first report of WNV identification from field-collected Cx. pseudovishnui and Mansonia uniformis in India. Our study demonstrates potential vectors which may play a crucial role in WNV transmission and should be considered in the vector control strategies. Additionally, our study highlights the role of sentinel chickens for WNV surveillance.</description><identifier>ISSN: 1756-3305</identifier><identifier>EISSN: 1756-3305</identifier><identifier>DOI: 10.1186/s13071-016-1948-9</identifier><identifier>PMID: 28061903</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Animals ; Antibodies, Viral - blood ; Chickens ; Culicidae - virology ; Disease Models, Animal ; Disease Transmission, Infectious ; Health aspects ; Identification and classification ; India ; Mosquitoes ; Phylogeny ; Prevalence studies (Epidemiology) ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Analysis, DNA ; Seroconversion ; West Nile Fever - transmission ; West Nile virus ; West Nile virus - isolation & purification</subject><ispartof>Parasites & vectors, 2017-01, Vol.10 (1), p.13-13, Article 13</ispartof><rights>COPYRIGHT 2017 BioMed Central Ltd.</rights><rights>Copyright BioMed Central 2017</rights><rights>The Author(s). 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c528t-dbd8f934e4317e298e7187a84407c75c0484f0b8cc3660ecdabbc4397cc96d833</citedby><cites>FETCH-LOGICAL-c528t-dbd8f934e4317e298e7187a84407c75c0484f0b8cc3660ecdabbc4397cc96d833</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5219652/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5219652/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,729,782,786,866,887,27931,27932,53798,53800</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28061903$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Khan, Siraj A</creatorcontrib><creatorcontrib>Chowdhury, Purvita</creatorcontrib><creatorcontrib>Choudhury, Parveena</creatorcontrib><creatorcontrib>Dutta, Prafulla</creatorcontrib><title>Detection of West Nile virus in six mosquito species in synchrony with seroconversion among sentinel chickens in India</title><title>Parasites & vectors</title><addtitle>Parasit Vectors</addtitle><description>West Nile virus (WNV) is a zoonotic flavivirus maintained in mosquito-bird transmission cycle. Although humans are accidental hosts, fatal outcomes following WNV infection have been reported from India. Studies have identified WNV as an important etiological agent causing acute encephalitis syndrome in Assam, Northeast India. While circulation of WNV is evident, the role of vectors and avian hosts involved in the transmission remains unclear. In this study we identified local mosquito species for evidence of WNV infection along with seroconversion among sentinel chickens.
Mosquitoes were collected and pooled species wise from June 2014 through December 2015. Virus was screened using reverse transcriptase PCR followed by sequencing and phylogenetic analysis. Sentinel chicken blood was screened for WNV antibody to assess their role in WNV transmission.
A total of 52,882 mosquitoes belonging to 16 species were collected. WNV was detected in 18 pools of Culex vishnui, Culex tritaeniorhynchus, Culex quinquefasciatus, Culex whitmorei, Culex pseudovishnui and Mansonia uniformis. Phylogenetic analysis revealed that all mosquito derived sequences belonged to Lineage 5 and were 99-100% similar to the Assam strain of WNV isolated from human CSF sample in 2007. All sentinel chickens had seroconverted by the month of July that happens to be the peak WNV transmission month among humans as well.
To the best of our knowledge, this is the first report of WNV identification from field-collected Cx. pseudovishnui and Mansonia uniformis in India. Our study demonstrates potential vectors which may play a crucial role in WNV transmission and should be considered in the vector control strategies. Additionally, our study highlights the role of sentinel chickens for WNV surveillance.</description><subject>Animals</subject><subject>Antibodies, Viral - blood</subject><subject>Chickens</subject><subject>Culicidae - virology</subject><subject>Disease Models, Animal</subject><subject>Disease Transmission, Infectious</subject><subject>Health aspects</subject><subject>Identification and classification</subject><subject>India</subject><subject>Mosquitoes</subject><subject>Phylogeny</subject><subject>Prevalence studies (Epidemiology)</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Sequence Analysis, DNA</subject><subject>Seroconversion</subject><subject>West Nile Fever - transmission</subject><subject>West Nile virus</subject><subject>West Nile virus - isolation & purification</subject><issn>1756-3305</issn><issn>1756-3305</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNptkktv1DAUhSMEoqXwA9ggS2zaRYodP2JvkKpSYKQKJB5iaTnOzYxLYk_tZOj8exymVDMIeWHr-jvH9vUpipcEnxMixZtEKK5JiYkoiWKyVI-KY1JzUVKK-eO99VHxLKUbjAVWXDwtjiqJBVGYHhebdzCCHV3wKHToB6QRfXI9oI2LU0LOo-Tu0BDS7eTGgNIarINdfevtKga_Rb_cuEIJYrDBbyCm2csMwS9z0Y_OQ4_sytmf4P8IF7515nnxpDN9ghf380nx_f3Vt8uP5fXnD4vLi-vS8kqOZdu0slOUAaOkhkpJqImsjWQM17bmFjPJOtxIa6kQGGxrmsYyqmprlWglpSfF253vemoGaG2-UDS9Xkc3mLjVwTh9uOPdSi_DRvOKKMGrbHB6bxDD7ZTboweXLPS98RCmpInkgivO1Iy-_ge9CVP0-XmZEpwyKas9aml60M53IZ9rZ1N9wWpRM4Ixz9T5f6g8Whhc7jN0-ZMOBWcHgsyMcDcuzZSSXnz9csiSHWtjSClC99APgvUcLL0Lls7B0nOwtMqaV_uNfFD8TRL9DS8vyQw</recordid><startdate>20170107</startdate><enddate>20170107</enddate><creator>Khan, Siraj A</creator><creator>Chowdhury, Purvita</creator><creator>Choudhury, Parveena</creator><creator>Dutta, Prafulla</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7SN</scope><scope>7SS</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H95</scope><scope>K9.</scope><scope>L.G</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20170107</creationdate><title>Detection of West Nile virus in six mosquito species in synchrony with seroconversion among sentinel chickens in India</title><author>Khan, Siraj A ; Chowdhury, Purvita ; Choudhury, Parveena ; Dutta, Prafulla</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c528t-dbd8f934e4317e298e7187a84407c75c0484f0b8cc3660ecdabbc4397cc96d833</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Antibodies, Viral - blood</topic><topic>Chickens</topic><topic>Culicidae - virology</topic><topic>Disease Models, Animal</topic><topic>Disease Transmission, Infectious</topic><topic>Health aspects</topic><topic>Identification and classification</topic><topic>India</topic><topic>Mosquitoes</topic><topic>Phylogeny</topic><topic>Prevalence studies (Epidemiology)</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Sequence Analysis, DNA</topic><topic>Seroconversion</topic><topic>West Nile Fever - transmission</topic><topic>West Nile virus</topic><topic>West Nile virus - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Khan, Siraj A</creatorcontrib><creatorcontrib>Chowdhury, Purvita</creatorcontrib><creatorcontrib>Choudhury, Parveena</creatorcontrib><creatorcontrib>Dutta, Prafulla</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Parasites & vectors</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Khan, Siraj A</au><au>Chowdhury, Purvita</au><au>Choudhury, Parveena</au><au>Dutta, Prafulla</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of West Nile virus in six mosquito species in synchrony with seroconversion among sentinel chickens in India</atitle><jtitle>Parasites & vectors</jtitle><addtitle>Parasit Vectors</addtitle><date>2017-01-07</date><risdate>2017</risdate><volume>10</volume><issue>1</issue><spage>13</spage><epage>13</epage><pages>13-13</pages><artnum>13</artnum><issn>1756-3305</issn><eissn>1756-3305</eissn><abstract>West Nile virus (WNV) is a zoonotic flavivirus maintained in mosquito-bird transmission cycle. Although humans are accidental hosts, fatal outcomes following WNV infection have been reported from India. Studies have identified WNV as an important etiological agent causing acute encephalitis syndrome in Assam, Northeast India. While circulation of WNV is evident, the role of vectors and avian hosts involved in the transmission remains unclear. In this study we identified local mosquito species for evidence of WNV infection along with seroconversion among sentinel chickens.
Mosquitoes were collected and pooled species wise from June 2014 through December 2015. Virus was screened using reverse transcriptase PCR followed by sequencing and phylogenetic analysis. Sentinel chicken blood was screened for WNV antibody to assess their role in WNV transmission.
A total of 52,882 mosquitoes belonging to 16 species were collected. WNV was detected in 18 pools of Culex vishnui, Culex tritaeniorhynchus, Culex quinquefasciatus, Culex whitmorei, Culex pseudovishnui and Mansonia uniformis. Phylogenetic analysis revealed that all mosquito derived sequences belonged to Lineage 5 and were 99-100% similar to the Assam strain of WNV isolated from human CSF sample in 2007. All sentinel chickens had seroconverted by the month of July that happens to be the peak WNV transmission month among humans as well.
To the best of our knowledge, this is the first report of WNV identification from field-collected Cx. pseudovishnui and Mansonia uniformis in India. Our study demonstrates potential vectors which may play a crucial role in WNV transmission and should be considered in the vector control strategies. Additionally, our study highlights the role of sentinel chickens for WNV surveillance.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>28061903</pmid><doi>10.1186/s13071-016-1948-9</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1756-3305 |
| ispartof | Parasites & vectors, 2017-01, Vol.10 (1), p.13-13, Article 13 |
| issn | 1756-3305 1756-3305 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5219652 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; SpringerNature Journals; PubMed Central; Springer Nature OA/Free Journals |
| subjects | Animals Antibodies, Viral - blood Chickens Culicidae - virology Disease Models, Animal Disease Transmission, Infectious Health aspects Identification and classification India Mosquitoes Phylogeny Prevalence studies (Epidemiology) Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis, DNA Seroconversion West Nile Fever - transmission West Nile virus West Nile virus - isolation & purification |
| title | Detection of West Nile virus in six mosquito species in synchrony with seroconversion among sentinel chickens in India |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-04T04%3A24%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Detection%20of%20West%20Nile%20virus%20in%20six%20mosquito%20species%20in%20synchrony%20with%20seroconversion%20among%20sentinel%20chickens%20in%20India&rft.jtitle=Parasites%20&%20vectors&rft.au=Khan,%20Siraj%20A&rft.date=2017-01-07&rft.volume=10&rft.issue=1&rft.spage=13&rft.epage=13&rft.pages=13-13&rft.artnum=13&rft.issn=1756-3305&rft.eissn=1756-3305&rft_id=info:doi/10.1186/s13071-016-1948-9&rft_dat=%3Cgale_pubme%3EA476741005%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1865348822&rft_id=info:pmid/28061903&rft_galeid=A476741005&rfr_iscdi=true |