Extraction of high-quality RNA from human articular cartilage

Extracting high-quality RNA from articular cartilage is challenging due to low cellularity and high proteoglycan content. This problem hinders efficient application of RNA sequencing (RNA-seq) analysis in studying cartilage homeostasis. Here we developed a method that purifies high-quality RNA direc...

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Veröffentlicht in:	Analytical biochemistry 2017-02, Vol.518, p.134-138
Hauptverfasser:	Le Bleu, Heather K., Kamal, Fadia A., Kelly, Meghan, Ketz, John P., Zuscik, Michael J., Elbarbary, Reyad A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Cartilage, Articular - chemistry Humans Knee Joint - chemistry Male RNA - chemistry RNA - isolation & purification
Online-Zugang:	Volltext
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