Sequence conservation of Plasmodium vivax glutamate dehydrogenase among Korean isolates and its application in seroepidemiology
Glutamate dehydrogenase of malaria parasites (pGDH) is widely used in rapid diagnostic tests for malaria. Variation in the pGDH gene among Korean isolates of Plasmodium vivax was analysed, and a recombinant pGDH protein was evaluated for use as antigens for the serodiagnosis of vivax malaria. Genomi...
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| Veröffentlicht in: | Malaria journal 2017-01, Vol.16 (1), p.3-3, Article 3 |
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| description | Glutamate dehydrogenase of malaria parasites (pGDH) is widely used in rapid diagnostic tests for malaria. Variation in the pGDH gene among Korean isolates of Plasmodium vivax was analysed, and a recombinant pGDH protein was evaluated for use as antigens for the serodiagnosis of vivax malaria.
Genomic DNA was purified from blood samples of 20 patients and the pGDH gene of P. vivax was sequenced. Recombinant protein was prepared to determine the antigenicity of pGDH by enzyme-linked immunosorbent assay (ELISA).
Partial sequence analysis of the P. vivax pGDH gene from the 20 Korean isolates showed that an open reading frame (ORF) of 1410 nucleotides encoded a deduced protein of 470 amino acids. The amino acid and nucleotide sequences were conserved among all the Korean isolates. This ORF showed 100% homology with P. vivax strain Sal-I (GenBank accession No. XP_001616617.1). The full ORF (amino acids 39-503), excluding the region before the intron, was cloned from isolate P. vivax Bucheon 3 (KJ726751) and subcloned into the expression vector pET28b for transformation into Escherichia coli BL21(DE3)pLysS. The expressed recombinant protein had a molecular mass of approximately 55 kDa and showed 84.8% sensitivity (39/46 cases) and 97.2% specificity (35/36 cases) in an ELISA. The efficacy of recombinant pGDH protein in seroepidemiological studies was also evaluated by ELISA using serum samples collected from 876 inhabitants of Gyodong-myeon, Ganghwa County, Incheon Metropolitan City. Of these samples, 91 (10.39%) showed a positive reaction with recombinant pGDH protein. Among the antibody-positive individuals, 13 (14.29%) had experienced malaria infection during the last 10 years.
The pGDH genes of P. vivax isolates from representative epidemic-prone areas of South Korea are highly conserved. Therefore, pGDH is expected to be a useful antigen in seroepidemiological studies. It was difficult to identify the foci of malaria transmission in Gyodong-myeon based on the patient distribution because of the very low parasitaemia of Korean vivax malaria. However, seroepidemiology with recombinant pGDH protein easily identified regions with the highest incidence of malaria within the study area. Therefore, recombinant pGDH protein may have a useful role in serodiagnosis. |
| doi_str_mv | 10.1186/s12936-016-1653-3 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5209832</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A507050951</galeid><sourcerecordid>A507050951</sourcerecordid><originalsourceid>FETCH-LOGICAL-c522t-64cfaea3af207f2f1f804a4d845b4d6a94a4fdd00f0a6809f0c754623eb5b0233</originalsourceid><addsrcrecordid>eNp9Ustu1TAQjRCIlsIHsEGW2LBJ8SN-ZINUVbxEJZCAtTU3GaeuHPsSJ1e9K34dR7eUFiHkxXjsM2c8x6eqnjN6yphRrzPjrVA1ZapmSopaPKiOWaNlzY2WD-_sj6onOV9RyrTR_HF1xA1t2ka3x9XPr_hjwdgh6VLMOO1g9imS5MiXAHlMvV9GsvM7uCZDWGYYYUbS4-W-n9KAETISGFMcyKc0IUTicwoFkgnEnvi5xO02-O7A6iMpLRJufY-jTyEN-6fVIwch47ObeFJ9f_f22_mH-uLz-4_nZxd1Jzmfa9V0DhAEOE614465MgE0vWnkpukVtCVxfU-po6AMbR3ttGwUF7iRG8qFOKneHHi3y2bEvsM4TxDsdvIjTHubwNv7N9Ff2iHtrOS0NYIXglc3BFMqiuXZjj53GAJETEu2zEgphNBaFujLv6BXaZliGc9y0RiuOGP8f6iVS2lplPmDGiCg9dGl8rpubW3PJNVU0laygjr9B6qsVefyseh8Ob9XwA4F3ZRyntDdKsGoXb1lD96yxVt29ZZdJXxxV8Lbit9mEr8AIvrLqw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1855675868</pqid></control><display><type>article</type><title>Sequence conservation of Plasmodium vivax glutamate dehydrogenase among Korean isolates and its application in seroepidemiology</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>SpringerNature Journals</source><source>PubMed Central Open Access</source><source>Springer Nature OA Free Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Seol, Bomin ; Shin, Hyun-Il ; Kim, Jung-Yeon ; Jeon, Bo-Young ; Kang, Yoon-Joong ; Pak, Jhang-Ho ; Kim, Tong-Soo ; Lee, Hyeong-Woo</creator><creatorcontrib>Seol, Bomin ; Shin, Hyun-Il ; Kim, Jung-Yeon ; Jeon, Bo-Young ; Kang, Yoon-Joong ; Pak, Jhang-Ho ; Kim, Tong-Soo ; Lee, Hyeong-Woo</creatorcontrib><description>Glutamate dehydrogenase of malaria parasites (pGDH) is widely used in rapid diagnostic tests for malaria. Variation in the pGDH gene among Korean isolates of Plasmodium vivax was analysed, and a recombinant pGDH protein was evaluated for use as antigens for the serodiagnosis of vivax malaria.
Genomic DNA was purified from blood samples of 20 patients and the pGDH gene of P. vivax was sequenced. Recombinant protein was prepared to determine the antigenicity of pGDH by enzyme-linked immunosorbent assay (ELISA).
Partial sequence analysis of the P. vivax pGDH gene from the 20 Korean isolates showed that an open reading frame (ORF) of 1410 nucleotides encoded a deduced protein of 470 amino acids. The amino acid and nucleotide sequences were conserved among all the Korean isolates. This ORF showed 100% homology with P. vivax strain Sal-I (GenBank accession No. XP_001616617.1). The full ORF (amino acids 39-503), excluding the region before the intron, was cloned from isolate P. vivax Bucheon 3 (KJ726751) and subcloned into the expression vector pET28b for transformation into Escherichia coli BL21(DE3)pLysS. The expressed recombinant protein had a molecular mass of approximately 55 kDa and showed 84.8% sensitivity (39/46 cases) and 97.2% specificity (35/36 cases) in an ELISA. The efficacy of recombinant pGDH protein in seroepidemiological studies was also evaluated by ELISA using serum samples collected from 876 inhabitants of Gyodong-myeon, Ganghwa County, Incheon Metropolitan City. Of these samples, 91 (10.39%) showed a positive reaction with recombinant pGDH protein. Among the antibody-positive individuals, 13 (14.29%) had experienced malaria infection during the last 10 years.
The pGDH genes of P. vivax isolates from representative epidemic-prone areas of South Korea are highly conserved. Therefore, pGDH is expected to be a useful antigen in seroepidemiological studies. It was difficult to identify the foci of malaria transmission in Gyodong-myeon based on the patient distribution because of the very low parasitaemia of Korean vivax malaria. However, seroepidemiology with recombinant pGDH protein easily identified regions with the highest incidence of malaria within the study area. Therefore, recombinant pGDH protein may have a useful role in serodiagnosis.</description><identifier>ISSN: 1475-2875</identifier><identifier>EISSN: 1475-2875</identifier><identifier>DOI: 10.1186/s12936-016-1653-3</identifier><identifier>PMID: 28049479</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Amino acids ; Antigenicity ; Antigens ; Causes of ; Conserved Sequence ; Dehydrogenase ; Dehydrogenases ; Deoxyribonucleic acid ; Diagnosis ; Disease transmission ; DNA ; DNA, Protozoan - chemistry ; DNA, Protozoan - genetics ; DNA, Protozoan - isolation & purification ; E coli ; ELISA ; Enzyme-linked immunosorbent assay ; Enzyme-Linked Immunosorbent Assay - methods ; Enzymes ; Escherichia coli ; Genes ; Genetic aspects ; Genetic Variation ; Genomics ; Glutamate dehydrogenase ; Glutamate Dehydrogenase - genetics ; Homology ; Human diseases ; Humans ; Malaria ; Malaria, Vivax - diagnosis ; Molecular weight ; Nucleotide sequence ; Nucleotides ; Open reading frames ; Parasites ; Patients ; Physiological aspects ; Plasmodium vivax ; Plasmodium vivax - enzymology ; Plasmodium vivax - genetics ; Proteins ; Recombinant Proteins - genetics ; Recombinant Proteins - isolation & purification ; Recombinant Proteins - metabolism ; Recombinants ; Republic of Korea ; Sequence analysis ; Sequence Analysis, DNA ; Sequencing ; Seroepidemiology ; Serologic Tests - methods ; Serum ; Specificity ; Vector-borne diseases</subject><ispartof>Malaria journal, 2017-01, Vol.16 (1), p.3-3, Article 3</ispartof><rights>COPYRIGHT 2017 BioMed Central Ltd.</rights><rights>Copyright BioMed Central 2017</rights><rights>2017. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>The Author(s) 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c522t-64cfaea3af207f2f1f804a4d845b4d6a94a4fdd00f0a6809f0c754623eb5b0233</citedby><cites>FETCH-LOGICAL-c522t-64cfaea3af207f2f1f804a4d845b4d6a94a4fdd00f0a6809f0c754623eb5b0233</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5209832/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5209832/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28049479$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Seol, Bomin</creatorcontrib><creatorcontrib>Shin, Hyun-Il</creatorcontrib><creatorcontrib>Kim, Jung-Yeon</creatorcontrib><creatorcontrib>Jeon, Bo-Young</creatorcontrib><creatorcontrib>Kang, Yoon-Joong</creatorcontrib><creatorcontrib>Pak, Jhang-Ho</creatorcontrib><creatorcontrib>Kim, Tong-Soo</creatorcontrib><creatorcontrib>Lee, Hyeong-Woo</creatorcontrib><title>Sequence conservation of Plasmodium vivax glutamate dehydrogenase among Korean isolates and its application in seroepidemiology</title><title>Malaria journal</title><addtitle>Malar J</addtitle><description>Glutamate dehydrogenase of malaria parasites (pGDH) is widely used in rapid diagnostic tests for malaria. Variation in the pGDH gene among Korean isolates of Plasmodium vivax was analysed, and a recombinant pGDH protein was evaluated for use as antigens for the serodiagnosis of vivax malaria.
Genomic DNA was purified from blood samples of 20 patients and the pGDH gene of P. vivax was sequenced. Recombinant protein was prepared to determine the antigenicity of pGDH by enzyme-linked immunosorbent assay (ELISA).
Partial sequence analysis of the P. vivax pGDH gene from the 20 Korean isolates showed that an open reading frame (ORF) of 1410 nucleotides encoded a deduced protein of 470 amino acids. The amino acid and nucleotide sequences were conserved among all the Korean isolates. This ORF showed 100% homology with P. vivax strain Sal-I (GenBank accession No. XP_001616617.1). The full ORF (amino acids 39-503), excluding the region before the intron, was cloned from isolate P. vivax Bucheon 3 (KJ726751) and subcloned into the expression vector pET28b for transformation into Escherichia coli BL21(DE3)pLysS. The expressed recombinant protein had a molecular mass of approximately 55 kDa and showed 84.8% sensitivity (39/46 cases) and 97.2% specificity (35/36 cases) in an ELISA. The efficacy of recombinant pGDH protein in seroepidemiological studies was also evaluated by ELISA using serum samples collected from 876 inhabitants of Gyodong-myeon, Ganghwa County, Incheon Metropolitan City. Of these samples, 91 (10.39%) showed a positive reaction with recombinant pGDH protein. Among the antibody-positive individuals, 13 (14.29%) had experienced malaria infection during the last 10 years.
The pGDH genes of P. vivax isolates from representative epidemic-prone areas of South Korea are highly conserved. Therefore, pGDH is expected to be a useful antigen in seroepidemiological studies. It was difficult to identify the foci of malaria transmission in Gyodong-myeon based on the patient distribution because of the very low parasitaemia of Korean vivax malaria. However, seroepidemiology with recombinant pGDH protein easily identified regions with the highest incidence of malaria within the study area. Therefore, recombinant pGDH protein may have a useful role in serodiagnosis.</description><subject>Amino acids</subject><subject>Antigenicity</subject><subject>Antigens</subject><subject>Causes of</subject><subject>Conserved Sequence</subject><subject>Dehydrogenase</subject><subject>Dehydrogenases</subject><subject>Deoxyribonucleic acid</subject><subject>Diagnosis</subject><subject>Disease transmission</subject><subject>DNA</subject><subject>DNA, Protozoan - chemistry</subject><subject>DNA, Protozoan - genetics</subject><subject>DNA, Protozoan - isolation & purification</subject><subject>E coli</subject><subject>ELISA</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Enzymes</subject><subject>Escherichia coli</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Genetic Variation</subject><subject>Genomics</subject><subject>Glutamate dehydrogenase</subject><subject>Glutamate Dehydrogenase - genetics</subject><subject>Homology</subject><subject>Human diseases</subject><subject>Humans</subject><subject>Malaria</subject><subject>Malaria, Vivax - diagnosis</subject><subject>Molecular weight</subject><subject>Nucleotide sequence</subject><subject>Nucleotides</subject><subject>Open reading frames</subject><subject>Parasites</subject><subject>Patients</subject><subject>Physiological aspects</subject><subject>Plasmodium vivax</subject><subject>Plasmodium vivax - enzymology</subject><subject>Plasmodium vivax - genetics</subject><subject>Proteins</subject><subject>Recombinant Proteins - genetics</subject><subject>Recombinant Proteins - isolation & purification</subject><subject>Recombinant Proteins - metabolism</subject><subject>Recombinants</subject><subject>Republic of Korea</subject><subject>Sequence analysis</subject><subject>Sequence Analysis, DNA</subject><subject>Sequencing</subject><subject>Seroepidemiology</subject><subject>Serologic Tests - methods</subject><subject>Serum</subject><subject>Specificity</subject><subject>Vector-borne diseases</subject><issn>1475-2875</issn><issn>1475-2875</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNp9Ustu1TAQjRCIlsIHsEGW2LBJ8SN-ZINUVbxEJZCAtTU3GaeuHPsSJ1e9K34dR7eUFiHkxXjsM2c8x6eqnjN6yphRrzPjrVA1ZapmSopaPKiOWaNlzY2WD-_sj6onOV9RyrTR_HF1xA1t2ka3x9XPr_hjwdgh6VLMOO1g9imS5MiXAHlMvV9GsvM7uCZDWGYYYUbS4-W-n9KAETISGFMcyKc0IUTicwoFkgnEnvi5xO02-O7A6iMpLRJufY-jTyEN-6fVIwch47ObeFJ9f_f22_mH-uLz-4_nZxd1Jzmfa9V0DhAEOE614465MgE0vWnkpukVtCVxfU-po6AMbR3ttGwUF7iRG8qFOKneHHi3y2bEvsM4TxDsdvIjTHubwNv7N9Ff2iHtrOS0NYIXglc3BFMqiuXZjj53GAJETEu2zEgphNBaFujLv6BXaZliGc9y0RiuOGP8f6iVS2lplPmDGiCg9dGl8rpubW3PJNVU0laygjr9B6qsVefyseh8Ob9XwA4F3ZRyntDdKsGoXb1lD96yxVt29ZZdJXxxV8Lbit9mEr8AIvrLqw</recordid><startdate>20170103</startdate><enddate>20170103</enddate><creator>Seol, Bomin</creator><creator>Shin, Hyun-Il</creator><creator>Kim, Jung-Yeon</creator><creator>Jeon, Bo-Young</creator><creator>Kang, Yoon-Joong</creator><creator>Pak, Jhang-Ho</creator><creator>Kim, Tong-Soo</creator><creator>Lee, Hyeong-Woo</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SS</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>H95</scope><scope>H97</scope><scope>K9.</scope><scope>L.G</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20170103</creationdate><title>Sequence conservation of Plasmodium vivax glutamate dehydrogenase among Korean isolates and its application in seroepidemiology</title><author>Seol, Bomin ; Shin, Hyun-Il ; Kim, Jung-Yeon ; Jeon, Bo-Young ; Kang, Yoon-Joong ; Pak, Jhang-Ho ; Kim, Tong-Soo ; Lee, Hyeong-Woo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c522t-64cfaea3af207f2f1f804a4d845b4d6a94a4fdd00f0a6809f0c754623eb5b0233</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Amino acids</topic><topic>Antigenicity</topic><topic>Antigens</topic><topic>Causes of</topic><topic>Conserved Sequence</topic><topic>Dehydrogenase</topic><topic>Dehydrogenases</topic><topic>Deoxyribonucleic acid</topic><topic>Diagnosis</topic><topic>Disease transmission</topic><topic>DNA</topic><topic>DNA, Protozoan - chemistry</topic><topic>DNA, Protozoan - genetics</topic><topic>DNA, Protozoan - isolation & purification</topic><topic>E coli</topic><topic>ELISA</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Enzymes</topic><topic>Escherichia coli</topic><topic>Genes</topic><topic>Genetic aspects</topic><topic>Genetic Variation</topic><topic>Genomics</topic><topic>Glutamate dehydrogenase</topic><topic>Glutamate Dehydrogenase - genetics</topic><topic>Homology</topic><topic>Human diseases</topic><topic>Humans</topic><topic>Malaria</topic><topic>Malaria, Vivax - diagnosis</topic><topic>Molecular weight</topic><topic>Nucleotide sequence</topic><topic>Nucleotides</topic><topic>Open reading frames</topic><topic>Parasites</topic><topic>Patients</topic><topic>Physiological aspects</topic><topic>Plasmodium vivax</topic><topic>Plasmodium vivax - enzymology</topic><topic>Plasmodium vivax - genetics</topic><topic>Proteins</topic><topic>Recombinant Proteins - genetics</topic><topic>Recombinant Proteins - isolation & purification</topic><topic>Recombinant Proteins - metabolism</topic><topic>Recombinants</topic><topic>Republic of Korea</topic><topic>Sequence analysis</topic><topic>Sequence Analysis, DNA</topic><topic>Sequencing</topic><topic>Seroepidemiology</topic><topic>Serologic Tests - methods</topic><topic>Serum</topic><topic>Specificity</topic><topic>Vector-borne diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Seol, Bomin</creatorcontrib><creatorcontrib>Shin, Hyun-Il</creatorcontrib><creatorcontrib>Kim, Jung-Yeon</creatorcontrib><creatorcontrib>Jeon, Bo-Young</creatorcontrib><creatorcontrib>Kang, Yoon-Joong</creatorcontrib><creatorcontrib>Pak, Jhang-Ho</creatorcontrib><creatorcontrib>Kim, Tong-Soo</creatorcontrib><creatorcontrib>Lee, Hyeong-Woo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Public Health Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Malaria journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Seol, Bomin</au><au>Shin, Hyun-Il</au><au>Kim, Jung-Yeon</au><au>Jeon, Bo-Young</au><au>Kang, Yoon-Joong</au><au>Pak, Jhang-Ho</au><au>Kim, Tong-Soo</au><au>Lee, Hyeong-Woo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sequence conservation of Plasmodium vivax glutamate dehydrogenase among Korean isolates and its application in seroepidemiology</atitle><jtitle>Malaria journal</jtitle><addtitle>Malar J</addtitle><date>2017-01-03</date><risdate>2017</risdate><volume>16</volume><issue>1</issue><spage>3</spage><epage>3</epage><pages>3-3</pages><artnum>3</artnum><issn>1475-2875</issn><eissn>1475-2875</eissn><abstract>Glutamate dehydrogenase of malaria parasites (pGDH) is widely used in rapid diagnostic tests for malaria. Variation in the pGDH gene among Korean isolates of Plasmodium vivax was analysed, and a recombinant pGDH protein was evaluated for use as antigens for the serodiagnosis of vivax malaria.
Genomic DNA was purified from blood samples of 20 patients and the pGDH gene of P. vivax was sequenced. Recombinant protein was prepared to determine the antigenicity of pGDH by enzyme-linked immunosorbent assay (ELISA).
Partial sequence analysis of the P. vivax pGDH gene from the 20 Korean isolates showed that an open reading frame (ORF) of 1410 nucleotides encoded a deduced protein of 470 amino acids. The amino acid and nucleotide sequences were conserved among all the Korean isolates. This ORF showed 100% homology with P. vivax strain Sal-I (GenBank accession No. XP_001616617.1). The full ORF (amino acids 39-503), excluding the region before the intron, was cloned from isolate P. vivax Bucheon 3 (KJ726751) and subcloned into the expression vector pET28b for transformation into Escherichia coli BL21(DE3)pLysS. The expressed recombinant protein had a molecular mass of approximately 55 kDa and showed 84.8% sensitivity (39/46 cases) and 97.2% specificity (35/36 cases) in an ELISA. The efficacy of recombinant pGDH protein in seroepidemiological studies was also evaluated by ELISA using serum samples collected from 876 inhabitants of Gyodong-myeon, Ganghwa County, Incheon Metropolitan City. Of these samples, 91 (10.39%) showed a positive reaction with recombinant pGDH protein. Among the antibody-positive individuals, 13 (14.29%) had experienced malaria infection during the last 10 years.
The pGDH genes of P. vivax isolates from representative epidemic-prone areas of South Korea are highly conserved. Therefore, pGDH is expected to be a useful antigen in seroepidemiological studies. It was difficult to identify the foci of malaria transmission in Gyodong-myeon based on the patient distribution because of the very low parasitaemia of Korean vivax malaria. However, seroepidemiology with recombinant pGDH protein easily identified regions with the highest incidence of malaria within the study area. Therefore, recombinant pGDH protein may have a useful role in serodiagnosis.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>28049479</pmid><doi>10.1186/s12936-016-1653-3</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; SpringerNature Journals; PubMed Central Open Access; Springer Nature OA Free Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Amino acids Antigenicity Antigens Causes of Conserved Sequence Dehydrogenase Dehydrogenases Deoxyribonucleic acid Diagnosis Disease transmission DNA DNA, Protozoan - chemistry DNA, Protozoan - genetics DNA, Protozoan - isolation & purification E coli ELISA Enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - methods Enzymes Escherichia coli Genes Genetic aspects Genetic Variation Genomics Glutamate dehydrogenase Glutamate Dehydrogenase - genetics Homology Human diseases Humans Malaria Malaria, Vivax - diagnosis Molecular weight Nucleotide sequence Nucleotides Open reading frames Parasites Patients Physiological aspects Plasmodium vivax Plasmodium vivax - enzymology Plasmodium vivax - genetics Proteins Recombinant Proteins - genetics Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism Recombinants Republic of Korea Sequence analysis Sequence Analysis, DNA Sequencing Seroepidemiology Serologic Tests - methods Serum Specificity Vector-borne diseases |
| title | Sequence conservation of Plasmodium vivax glutamate dehydrogenase among Korean isolates and its application in seroepidemiology |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-19T17%3A23%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Sequence%20conservation%20of%20Plasmodium%20vivax%20glutamate%20dehydrogenase%20among%20Korean%20isolates%20and%20its%20application%20in%20seroepidemiology&rft.jtitle=Malaria%20journal&rft.au=Seol,%20Bomin&rft.date=2017-01-03&rft.volume=16&rft.issue=1&rft.spage=3&rft.epage=3&rft.pages=3-3&rft.artnum=3&rft.issn=1475-2875&rft.eissn=1475-2875&rft_id=info:doi/10.1186/s12936-016-1653-3&rft_dat=%3Cgale_pubme%3EA507050951%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1855675868&rft_id=info:pmid/28049479&rft_galeid=A507050951&rfr_iscdi=true |