Detection of Isoforms Differing by a Single Charge Unit in Individual Cells

To measure protein isoforms in individual mammalian cells, we report single‐cell resolution isoelectric focusing (scIEF) and high‐selectivity immunoprobing. Microfluidic design and photoactivatable materials establish the tunable pH gradients required by IEF and precisely control the transport and h...

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Veröffentlicht in:	Angewandte Chemie International Edition 2016-09, Vol.55 (40), p.12431-12435
Hauptverfasser:	Tentori, Augusto M., Yamauchi, Kevin A., Herr, Amy E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Assaying Cell Line, Tumor Design Design analysis Fluorescent Dyes - chemistry Humans Hydrogen-Ion Concentration Immunoassay immunoassays Isoelectric focusing Isoelectric Focusing - methods Isoforms lab-on-a-chip Lab-On-A-Chip Devices Mammalian cells Mammals Materials selection Microfluidics Protein Isoforms - analysis Proteins proteomics Selectivity Single-Cell Analysis Transport
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