The Montpellier Leishmania Collection, from a Laboratory Collection to a Biological Resource Center: A 39-Year-Long Story

We report the development of a laboratory collection of Leishmania that was initiated in 1975 and, after 39 years, has become an international Biological Resource Center (BRC-Leish, Montpellier, France, BioBank No. BB-0033-00052), which includes 6353 strains belonging to 36 Leishmania taxa. This is...

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Veröffentlicht in:Biopreservation and biobanking 2016-12, Vol.14 (6), p.470-479
Hauptverfasser: Pratlong, Francine, Balard, Yves, Lami, Patrick, Talignani, Loïc, Ravel, Christophe, Dereure, Jacques, Lefebvre, Michèle, Serres, Ghislaine, Bastien, Patrick, Dedet, Jean-Pierre
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container_issue 6
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container_title Biopreservation and biobanking
container_volume 14
creator Pratlong, Francine
Balard, Yves
Lami, Patrick
Talignani, Loïc
Ravel, Christophe
Dereure, Jacques
Lefebvre, Michèle
Serres, Ghislaine
Bastien, Patrick
Dedet, Jean-Pierre
description We report the development of a laboratory collection of Leishmania that was initiated in 1975 and, after 39 years, has become an international Biological Resource Center (BRC-Leish, Montpellier, France, BioBank No. BB-0033-00052), which includes 6353 strains belonging to 36 Leishmania taxa. This is a retrospective analysis of the technical and organizational changes that have been adopted over time to take into account the technological advances and related modifications in the collection management and quality system. The technical improvements concerned the culture and cryopreservation techniques, strain identification by isoenzymatic and molecular techniques, data computerization and quality management to meet the changes in international standards, and in the cryogenic and microbiological safety procedures. The BRC is working toward obtaining the NF-S 96-900 certification in the coming years. Our long-term expertise in Leishmania storage and typing and collection maintenance should encourage field epidemiologists and clinical practitioners in endemic countries to secure their own strain collection with the help of the French BRC-Leish.
doi_str_mv 10.1089/bio.2015.0101
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source MEDLINE; Alma/SFX Local Collection
subjects Biological Specimen Banks - standards
Biological Specimen Banks - trends
Cellular Biology
Cryopreservation
Humans
Leishmania - classification
Leishmania - growth & development
Life Sciences
Microbiological Techniques
Original
Retrospective Studies
Specimen Handling - standards
Specimen Handling - trends
title The Montpellier Leishmania Collection, from a Laboratory Collection to a Biological Resource Center: A 39-Year-Long Story
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