PATHFAST, a novel chemiluminescent enzyme immunoassay for measuring estradiol in equine whole blood and serum
A novel chemiluminescent enzyme immunoassay system, PATHFAST, for the measurement of estradiol in horses was evaluated. The concentrations of estradiol in the whole blood and serum of mares were measured using PATHFAST and the estradiol concentrations measured by PATHFAST were compared with those me...
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| Veröffentlicht in: | Journal of Reproduction and Development 2016, Vol.62(6), pp.631-634 |
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| container_end_page | 634 |
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| container_issue | 6 |
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| container_title | Journal of Reproduction and Development |
| container_volume | 62 |
| creator | TOISHI, Yuko TSUNODA, Nobuo KUME, Kouiti NAGAOKA, Kentaro WATANABE, Gen TAYA, Kazuyoshi |
| description | A novel chemiluminescent enzyme immunoassay system, PATHFAST, for the measurement of estradiol in horses was evaluated. The concentrations of estradiol in the whole blood and serum of mares were measured using PATHFAST and the estradiol concentrations measured by PATHFAST were compared with those measured by a time-resolved fluoro-immunoassay (FIA). To monitor physiological changes, serum estradiol concentrations in mares were measured using PATHFAST throughout the gestation period. The serum estradiol concentrations correlated highly with those in whole blood samples. The serum concentrations of estradiol measured by PATHFAST also correlated well with FIA. Circulating estradiol increased during mid-gestation and high levels of serum estradiol were maintained in late gestation, followed by an abrupt decline to term. These results demonstrate the utility of PATHFAST in equine clinics as an accurate diagnostic tool for the rapid assay of estradiol within 26 min using unextracted whole blood. |
| doi_str_mv | 10.1262/jrd.2016-038 |
| format | Article |
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The concentrations of estradiol in the whole blood and serum of mares were measured using PATHFAST and the estradiol concentrations measured by PATHFAST were compared with those measured by a time-resolved fluoro-immunoassay (FIA). To monitor physiological changes, serum estradiol concentrations in mares were measured using PATHFAST throughout the gestation period. The serum estradiol concentrations correlated highly with those in whole blood samples. The serum concentrations of estradiol measured by PATHFAST also correlated well with FIA. Circulating estradiol increased during mid-gestation and high levels of serum estradiol were maintained in late gestation, followed by an abrupt decline to term. These results demonstrate the utility of PATHFAST in equine clinics as an accurate diagnostic tool for the rapid assay of estradiol within 26 min using unextracted whole blood.</description><identifier>ISSN: 0916-8818</identifier><identifier>EISSN: 1348-4400</identifier><identifier>DOI: 10.1262/jrd.2016-038</identifier><identifier>PMID: 27545960</identifier><language>eng</language><publisher>Japan: THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT</publisher><subject>Animals ; Chemiluminescent enzyme immunoassay ; Estradiol ; Estradiol - blood ; Horses ; Horses - blood ; Immunoenzyme Techniques - methods ; Immunoenzyme Techniques - veterinary ; Luminescent Measurements - methods ; Luminescent Measurements - veterinary ; PATHFAST ; Technology Report ; Time-resolved fluoro-immunoassay</subject><ispartof>Journal of Reproduction and Development, 2016, Vol.62(6), pp.631-634</ispartof><rights>2016 Society for Reproduction and Development</rights><rights>2016 Society for Reproduction and Development 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c650t-ee1edbd4ee4420149cc0120885e32f06a03abb8e40b55ce0f04b719a43f2f60a3</citedby><cites>FETCH-LOGICAL-c650t-ee1edbd4ee4420149cc0120885e32f06a03abb8e40b55ce0f04b719a43f2f60a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5177982/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5177982/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,1877,4010,27900,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27545960$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>TOISHI, Yuko</creatorcontrib><creatorcontrib>TSUNODA, Nobuo</creatorcontrib><creatorcontrib>KUME, Kouiti</creatorcontrib><creatorcontrib>NAGAOKA, Kentaro</creatorcontrib><creatorcontrib>WATANABE, Gen</creatorcontrib><creatorcontrib>TAYA, Kazuyoshi</creatorcontrib><title>PATHFAST, a novel chemiluminescent enzyme immunoassay for measuring estradiol in equine whole blood and serum</title><title>Journal of Reproduction and Development</title><addtitle>J. Reprod. Dev.</addtitle><description>A novel chemiluminescent enzyme immunoassay system, PATHFAST, for the measurement of estradiol in horses was evaluated. The concentrations of estradiol in the whole blood and serum of mares were measured using PATHFAST and the estradiol concentrations measured by PATHFAST were compared with those measured by a time-resolved fluoro-immunoassay (FIA). To monitor physiological changes, serum estradiol concentrations in mares were measured using PATHFAST throughout the gestation period. The serum estradiol concentrations correlated highly with those in whole blood samples. The serum concentrations of estradiol measured by PATHFAST also correlated well with FIA. Circulating estradiol increased during mid-gestation and high levels of serum estradiol were maintained in late gestation, followed by an abrupt decline to term. These results demonstrate the utility of PATHFAST in equine clinics as an accurate diagnostic tool for the rapid assay of estradiol within 26 min using unextracted whole blood.</description><subject>Animals</subject><subject>Chemiluminescent enzyme immunoassay</subject><subject>Estradiol</subject><subject>Estradiol - blood</subject><subject>Horses</subject><subject>Horses - blood</subject><subject>Immunoenzyme Techniques - methods</subject><subject>Immunoenzyme Techniques - veterinary</subject><subject>Luminescent Measurements - methods</subject><subject>Luminescent Measurements - veterinary</subject><subject>PATHFAST</subject><subject>Technology Report</subject><subject>Time-resolved fluoro-immunoassay</subject><issn>0916-8818</issn><issn>1348-4400</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkU1vEzEQhi0EoqFw44x85NAt47XX8V6QQkUpUiWQCGfLuzubOPJHa-8WhV-PQ9oILrbkefyMxy8hbxlcslrWH3ZpuKyByQq4ekYWjAtVCQHwnCygLcdKMXVGXuW8A-B1I8VLclYvG9G0EhbEf1-tb65XP9YX1NAQH9DRfoveutnbgLnHMFEMv_ceqfV-DtHkbPZ0jIl6NHlONmwo5imZwUZHbaB4P5eb9Nc2OqSdi3GgJgw0Y5r9a_JiNC7jm8f9nPy8_ry-uqluv335erW6rXrZwFQhMhy6QSAKUUYTbd8Dq0GpBnk9gjTATdcpFNA1TY8wguiWrDWCj_UowfBz8vHovZs7j8NhimScvkvWm7TX0Vj9fyXYrd7EB92w5bJVdRG8fxSkeD-X-bS35TOcMwHjnDVTUnEmGiYLenFE-xRzTjie2jDQh4R0SUgfEtIloYK_-_dpJ_gpkgJ8OgK7PJkNngCTJts7_GsrUnlYnqynYr81SWPgfwD2FKan</recordid><startdate>2016</startdate><enddate>2016</enddate><creator>TOISHI, Yuko</creator><creator>TSUNODA, Nobuo</creator><creator>KUME, Kouiti</creator><creator>NAGAOKA, Kentaro</creator><creator>WATANABE, Gen</creator><creator>TAYA, Kazuyoshi</creator><general>THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT</general><general>The Society for Reproduction and Development</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>5PM</scope></search><sort><creationdate>2016</creationdate><title>PATHFAST, a novel chemiluminescent enzyme immunoassay for measuring estradiol in equine whole blood and serum</title><author>TOISHI, Yuko ; TSUNODA, Nobuo ; KUME, Kouiti ; NAGAOKA, Kentaro ; WATANABE, Gen ; TAYA, Kazuyoshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c650t-ee1edbd4ee4420149cc0120885e32f06a03abb8e40b55ce0f04b719a43f2f60a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Chemiluminescent enzyme immunoassay</topic><topic>Estradiol</topic><topic>Estradiol - blood</topic><topic>Horses</topic><topic>Horses - blood</topic><topic>Immunoenzyme Techniques - methods</topic><topic>Immunoenzyme Techniques - veterinary</topic><topic>Luminescent Measurements - methods</topic><topic>Luminescent Measurements - veterinary</topic><topic>PATHFAST</topic><topic>Technology Report</topic><topic>Time-resolved fluoro-immunoassay</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>TOISHI, Yuko</creatorcontrib><creatorcontrib>TSUNODA, Nobuo</creatorcontrib><creatorcontrib>KUME, Kouiti</creatorcontrib><creatorcontrib>NAGAOKA, Kentaro</creatorcontrib><creatorcontrib>WATANABE, Gen</creatorcontrib><creatorcontrib>TAYA, Kazuyoshi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Reproduction and Development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>TOISHI, Yuko</au><au>TSUNODA, Nobuo</au><au>KUME, Kouiti</au><au>NAGAOKA, Kentaro</au><au>WATANABE, Gen</au><au>TAYA, Kazuyoshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>PATHFAST, a novel chemiluminescent enzyme immunoassay for measuring estradiol in equine whole blood and serum</atitle><jtitle>Journal of Reproduction and Development</jtitle><addtitle>J. Reprod. Dev.</addtitle><date>2016</date><risdate>2016</risdate><volume>62</volume><issue>6</issue><spage>631</spage><epage>634</epage><pages>631-634</pages><issn>0916-8818</issn><eissn>1348-4400</eissn><abstract>A novel chemiluminescent enzyme immunoassay system, PATHFAST, for the measurement of estradiol in horses was evaluated. The concentrations of estradiol in the whole blood and serum of mares were measured using PATHFAST and the estradiol concentrations measured by PATHFAST were compared with those measured by a time-resolved fluoro-immunoassay (FIA). To monitor physiological changes, serum estradiol concentrations in mares were measured using PATHFAST throughout the gestation period. The serum estradiol concentrations correlated highly with those in whole blood samples. The serum concentrations of estradiol measured by PATHFAST also correlated well with FIA. Circulating estradiol increased during mid-gestation and high levels of serum estradiol were maintained in late gestation, followed by an abrupt decline to term. These results demonstrate the utility of PATHFAST in equine clinics as an accurate diagnostic tool for the rapid assay of estradiol within 26 min using unextracted whole blood.</abstract><cop>Japan</cop><pub>THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT</pub><pmid>27545960</pmid><doi>10.1262/jrd.2016-038</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese; EZB-FREE-00999 freely available EZB journals; PubMed Central; PubMed Central Open Access |
| subjects | Animals Chemiluminescent enzyme immunoassay Estradiol Estradiol - blood Horses Horses - blood Immunoenzyme Techniques - methods Immunoenzyme Techniques - veterinary Luminescent Measurements - methods Luminescent Measurements - veterinary PATHFAST Technology Report Time-resolved fluoro-immunoassay |
| title | PATHFAST, a novel chemiluminescent enzyme immunoassay for measuring estradiol in equine whole blood and serum |
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