Kif2a regulates spindle organization and cell cycle progression in meiotic oocytes
Kif2a is a member of the Kinesin-13 microtubule depolymerases. Here, we report the expression, subcellular localization and functions of Kif2a during mouse oocyte meiotic maturation. Immunoblotting analysis showed that Kif2a was gradually increased form GV to the M I stages, and then decreased sligh...
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| Veröffentlicht in: | Scientific reports 2016-12, Vol.6 (1), p.38574-38574, Article 38574 |
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| creator | Yi, Zi-Yun Ma, Xue-Shan Liang, Qiu-Xia Zhang, Teng Xu, Zhao-Yang Meng, Tie-Gang Ouyang, Ying-Chun Hou, Yi Schatten, Heide Sun, Qing-Yuan Quan, Song |
| description | Kif2a is a member of the Kinesin-13 microtubule depolymerases. Here, we report the expression, subcellular localization and functions of Kif2a during mouse oocyte meiotic maturation. Immunoblotting analysis showed that Kif2a was gradually increased form GV to the M I stages, and then decreased slightly at the M II stage. Confocal microscopy identified that Kif2a localized to the meiotic spindle, especially concentrated at the spindle poles and inner centromeres in metaphase and translocated to the midbody at telophase. Kif2a depletion by siRNA microinjection generated severely defective spindles and misaligned chromosomes, reduced microtubule depolymerization, which led to significant pro-M I/M Iarrest and failure of first polar body (PB1) extrusion. Kif2a-depleted oocytes were also defective in spindle pole localization of γ-tubulin and showed spindle assembly checkpoint (SAC) protein Bub3 at the kinetochores even after 10 hr extended culture. These results demonstrate that Kif2a may act as a microtubule depolymerase, regulating microtubule dynamics, spindle assembly and chromosome congression, and thus cell cycle progression during mouse oocyte meiotic maturation. |
| doi_str_mv | 10.1038/srep38574 |
| format | Article |
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Here, we report the expression, subcellular localization and functions of Kif2a during mouse oocyte meiotic maturation. Immunoblotting analysis showed that Kif2a was gradually increased form GV to the M I stages, and then decreased slightly at the M II stage. Confocal microscopy identified that Kif2a localized to the meiotic spindle, especially concentrated at the spindle poles and inner centromeres in metaphase and translocated to the midbody at telophase. Kif2a depletion by siRNA microinjection generated severely defective spindles and misaligned chromosomes, reduced microtubule depolymerization, which led to significant pro-M I/M Iarrest and failure of first polar body (PB1) extrusion. Kif2a-depleted oocytes were also defective in spindle pole localization of γ-tubulin and showed spindle assembly checkpoint (SAC) protein Bub3 at the kinetochores even after 10 hr extended culture. These results demonstrate that Kif2a may act as a microtubule depolymerase, regulating microtubule dynamics, spindle assembly and chromosome congression, and thus cell cycle progression during mouse oocyte meiotic maturation.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep38574</identifier><identifier>PMID: 27991495</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13/89 ; 14/19 ; 631/136/83 ; 631/80/83 ; 82/80 ; Animals ; Cell culture ; Cell cycle ; Cell Differentiation - drug effects ; Centromeres ; Chromosome Segregation - drug effects ; Chromosomes ; Chromosomes, Mammalian - metabolism ; Confocal microscopy ; Depolymerization ; Female ; Gene Knockdown Techniques ; Humanities and Social Sciences ; Immunoblotting ; Kinesin ; Kinesin - metabolism ; Kinetochores ; Localization ; M Phase Cell Cycle Checkpoints - drug effects ; Meiosis ; Meiosis - drug effects ; Metaphase ; Mice, Inbred ICR ; Microinjection ; multidisciplinary ; Nocodazole - pharmacology ; Oocytes ; Oocytes - cytology ; Oocytes - drug effects ; Oocytes - metabolism ; Paclitaxel - pharmacology ; Polar Bodies - metabolism ; Repressor Proteins - metabolism ; Science ; siRNA ; Spindle Apparatus - drug effects ; Spindle Apparatus - metabolism ; Spindles ; Subcellular Fractions - drug effects ; Subcellular Fractions - metabolism ; Tubulin ; Tubulin - metabolism</subject><ispartof>Scientific reports, 2016-12, Vol.6 (1), p.38574-38574, Article 38574</ispartof><rights>The Author(s) 2016</rights><rights>Copyright Nature Publishing Group Dec 2016</rights><rights>Copyright © 2016, The Author(s) 2016 The Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c504t-adc4207bafeb2adc8e9cc18667ed76063a63b56f1e661fa7dfa36a245cd746263</citedby><cites>FETCH-LOGICAL-c504t-adc4207bafeb2adc8e9cc18667ed76063a63b56f1e661fa7dfa36a245cd746263</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5171826/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5171826/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,27905,27906,41101,42170,51557,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27991495$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yi, Zi-Yun</creatorcontrib><creatorcontrib>Ma, Xue-Shan</creatorcontrib><creatorcontrib>Liang, Qiu-Xia</creatorcontrib><creatorcontrib>Zhang, Teng</creatorcontrib><creatorcontrib>Xu, Zhao-Yang</creatorcontrib><creatorcontrib>Meng, Tie-Gang</creatorcontrib><creatorcontrib>Ouyang, Ying-Chun</creatorcontrib><creatorcontrib>Hou, Yi</creatorcontrib><creatorcontrib>Schatten, Heide</creatorcontrib><creatorcontrib>Sun, Qing-Yuan</creatorcontrib><creatorcontrib>Quan, Song</creatorcontrib><title>Kif2a regulates spindle organization and cell cycle progression in meiotic oocytes</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Kif2a is a member of the Kinesin-13 microtubule depolymerases. Here, we report the expression, subcellular localization and functions of Kif2a during mouse oocyte meiotic maturation. Immunoblotting analysis showed that Kif2a was gradually increased form GV to the M I stages, and then decreased slightly at the M II stage. Confocal microscopy identified that Kif2a localized to the meiotic spindle, especially concentrated at the spindle poles and inner centromeres in metaphase and translocated to the midbody at telophase. Kif2a depletion by siRNA microinjection generated severely defective spindles and misaligned chromosomes, reduced microtubule depolymerization, which led to significant pro-M I/M Iarrest and failure of first polar body (PB1) extrusion. Kif2a-depleted oocytes were also defective in spindle pole localization of γ-tubulin and showed spindle assembly checkpoint (SAC) protein Bub3 at the kinetochores even after 10 hr extended culture. These results demonstrate that Kif2a may act as a microtubule depolymerase, regulating microtubule dynamics, spindle assembly and chromosome congression, and thus cell cycle progression during mouse oocyte meiotic maturation.</description><subject>13/89</subject><subject>14/19</subject><subject>631/136/83</subject><subject>631/80/83</subject><subject>82/80</subject><subject>Animals</subject><subject>Cell culture</subject><subject>Cell cycle</subject><subject>Cell Differentiation - drug effects</subject><subject>Centromeres</subject><subject>Chromosome Segregation - drug effects</subject><subject>Chromosomes</subject><subject>Chromosomes, Mammalian - metabolism</subject><subject>Confocal microscopy</subject><subject>Depolymerization</subject><subject>Female</subject><subject>Gene Knockdown Techniques</subject><subject>Humanities and Social Sciences</subject><subject>Immunoblotting</subject><subject>Kinesin</subject><subject>Kinesin - metabolism</subject><subject>Kinetochores</subject><subject>Localization</subject><subject>M Phase Cell Cycle Checkpoints - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yi, Zi-Yun</au><au>Ma, Xue-Shan</au><au>Liang, Qiu-Xia</au><au>Zhang, Teng</au><au>Xu, Zhao-Yang</au><au>Meng, Tie-Gang</au><au>Ouyang, Ying-Chun</au><au>Hou, Yi</au><au>Schatten, Heide</au><au>Sun, Qing-Yuan</au><au>Quan, Song</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Kif2a regulates spindle organization and cell cycle progression in meiotic oocytes</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2016-12-19</date><risdate>2016</risdate><volume>6</volume><issue>1</issue><spage>38574</spage><epage>38574</epage><pages>38574-38574</pages><artnum>38574</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Kif2a is a member of the Kinesin-13 microtubule depolymerases. Here, we report the expression, subcellular localization and functions of Kif2a during mouse oocyte meiotic maturation. Immunoblotting analysis showed that Kif2a was gradually increased form GV to the M I stages, and then decreased slightly at the M II stage. Confocal microscopy identified that Kif2a localized to the meiotic spindle, especially concentrated at the spindle poles and inner centromeres in metaphase and translocated to the midbody at telophase. Kif2a depletion by siRNA microinjection generated severely defective spindles and misaligned chromosomes, reduced microtubule depolymerization, which led to significant pro-M I/M Iarrest and failure of first polar body (PB1) extrusion. Kif2a-depleted oocytes were also defective in spindle pole localization of γ-tubulin and showed spindle assembly checkpoint (SAC) protein Bub3 at the kinetochores even after 10 hr extended culture. These results demonstrate that Kif2a may act as a microtubule depolymerase, regulating microtubule dynamics, spindle assembly and chromosome congression, and thus cell cycle progression during mouse oocyte meiotic maturation.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>27991495</pmid><doi>10.1038/srep38574</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 13/89 14/19 631/136/83 631/80/83 82/80 Animals Cell culture Cell cycle Cell Differentiation - drug effects Centromeres Chromosome Segregation - drug effects Chromosomes Chromosomes, Mammalian - metabolism Confocal microscopy Depolymerization Female Gene Knockdown Techniques Humanities and Social Sciences Immunoblotting Kinesin Kinesin - metabolism Kinetochores Localization M Phase Cell Cycle Checkpoints - drug effects Meiosis Meiosis - drug effects Metaphase Mice, Inbred ICR Microinjection multidisciplinary Nocodazole - pharmacology Oocytes Oocytes - cytology Oocytes - drug effects Oocytes - metabolism Paclitaxel - pharmacology Polar Bodies - metabolism Repressor Proteins - metabolism Science siRNA Spindle Apparatus - drug effects Spindle Apparatus - metabolism Spindles Subcellular Fractions - drug effects Subcellular Fractions - metabolism Tubulin Tubulin - metabolism |
| title | Kif2a regulates spindle organization and cell cycle progression in meiotic oocytes |
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