Serine/Arginine-rich Splicing Factor 2 Modulates Herpes Simplex Virus Type 1 Replication via Regulating Viral Gene Transcriptional Activity and Pre-mRNA Splicing
Once it enters the host cell, herpes simplex virus type 1 (HSV-1) recruits a series of host cell factors to facilitate its life cycle. Here, we demonstrate that serine/arginine-rich splicing factor 2 (SRSF2), which is an important component of the splicing speckle, mediates HSV-1 replication by regu...
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| Veröffentlicht in: | The Journal of biological chemistry 2016-12, Vol.291 (51), p.26377-26387 |
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| creator | Wang, Ziqiang Liu, Qing Lu, Jinhua Fan, Ping Xie, Weidong Qiu, Wei Wang, Fan Hu, Guangnan Zhang, Yaou |
| description | Once it enters the host cell, herpes simplex virus type 1 (HSV-1) recruits a series of host cell factors to facilitate its life cycle. Here, we demonstrate that serine/arginine-rich splicing factor 2 (SRSF2), which is an important component of the splicing speckle, mediates HSV-1 replication by regulating viral gene expression at the transcriptional and posttranscriptional levels. Our results indicate that SRSF2 functions as a transcriptional activator by directly binding to infected cell polypeptide 0 (ICP0), infected cell polypeptide 27 (ICP27), and thymidine kinase promoters. Moreover, SRSF2 participates in ICP0 pre-mRNA splicing by recognizing binding sites in ICP0 exon 3. These findings provide insight into the functions of SRSF2 in HSV-1 replication and gene expression. |
| doi_str_mv | 10.1074/jbc.M116.753046 |
| format | Article |
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Here, we demonstrate that serine/arginine-rich splicing factor 2 (SRSF2), which is an important component of the splicing speckle, mediates HSV-1 replication by regulating viral gene expression at the transcriptional and posttranscriptional levels. Our results indicate that SRSF2 functions as a transcriptional activator by directly binding to infected cell polypeptide 0 (ICP0), infected cell polypeptide 27 (ICP27), and thymidine kinase promoters. Moreover, SRSF2 participates in ICP0 pre-mRNA splicing by recognizing binding sites in ICP0 exon 3. These findings provide insight into the functions of SRSF2 in HSV-1 replication and gene expression.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1074/jbc.M116.753046</identifier><identifier>PMID: 27784784</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Chlorocebus aethiops ; gene expression ; Gene Expression Regulation, Viral - physiology ; HeLa Cells ; herpesvirus ; Herpesvirus 1, Human - physiology ; host-pathogen interaction ; Humans ; Immediate-Early Proteins - biosynthesis ; Immediate-Early Proteins - genetics ; Microbiology ; RNA Precursors - biosynthesis ; RNA Precursors - genetics ; RNA splicing ; RNA Splicing - physiology ; RNA, Viral - biosynthesis ; RNA, Viral - genetics ; Serine-Arginine Splicing Factors - genetics ; Serine-Arginine Splicing Factors - metabolism ; transcription factor ; Transcription, Genetic - physiology ; Ubiquitin-Protein Ligases - biosynthesis ; Ubiquitin-Protein Ligases - genetics ; Vero Cells ; Virus Replication - physiology</subject><ispartof>The Journal of biological chemistry, 2016-12, Vol.291 (51), p.26377-26387</ispartof><rights>2016 © 2016 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.</rights><rights>2016 by The American Society for Biochemistry and Molecular Biology, Inc.</rights><rights>2016 by The American Society for Biochemistry and Molecular Biology, Inc. 2016 The American Society for Biochemistry and Molecular Biology, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c443t-1b6da4262bc58581bb7efd6198186e49b4155a30b5afbc7df707c8884478271b3</citedby><cites>FETCH-LOGICAL-c443t-1b6da4262bc58581bb7efd6198186e49b4155a30b5afbc7df707c8884478271b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5159499/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5159499/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27915,27916,53782,53784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27784784$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Ziqiang</creatorcontrib><creatorcontrib>Liu, Qing</creatorcontrib><creatorcontrib>Lu, Jinhua</creatorcontrib><creatorcontrib>Fan, Ping</creatorcontrib><creatorcontrib>Xie, Weidong</creatorcontrib><creatorcontrib>Qiu, Wei</creatorcontrib><creatorcontrib>Wang, Fan</creatorcontrib><creatorcontrib>Hu, Guangnan</creatorcontrib><creatorcontrib>Zhang, Yaou</creatorcontrib><title>Serine/Arginine-rich Splicing Factor 2 Modulates Herpes Simplex Virus Type 1 Replication via Regulating Viral Gene Transcriptional Activity and Pre-mRNA Splicing</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>Once it enters the host cell, herpes simplex virus type 1 (HSV-1) recruits a series of host cell factors to facilitate its life cycle. Here, we demonstrate that serine/arginine-rich splicing factor 2 (SRSF2), which is an important component of the splicing speckle, mediates HSV-1 replication by regulating viral gene expression at the transcriptional and posttranscriptional levels. Our results indicate that SRSF2 functions as a transcriptional activator by directly binding to infected cell polypeptide 0 (ICP0), infected cell polypeptide 27 (ICP27), and thymidine kinase promoters. Moreover, SRSF2 participates in ICP0 pre-mRNA splicing by recognizing binding sites in ICP0 exon 3. These findings provide insight into the functions of SRSF2 in HSV-1 replication and gene expression.</description><subject>Animals</subject><subject>Chlorocebus aethiops</subject><subject>gene expression</subject><subject>Gene Expression Regulation, Viral - physiology</subject><subject>HeLa Cells</subject><subject>herpesvirus</subject><subject>Herpesvirus 1, Human - physiology</subject><subject>host-pathogen interaction</subject><subject>Humans</subject><subject>Immediate-Early Proteins - biosynthesis</subject><subject>Immediate-Early Proteins - genetics</subject><subject>Microbiology</subject><subject>RNA Precursors - biosynthesis</subject><subject>RNA Precursors - genetics</subject><subject>RNA splicing</subject><subject>RNA Splicing - physiology</subject><subject>RNA, Viral - biosynthesis</subject><subject>RNA, Viral - genetics</subject><subject>Serine-Arginine Splicing Factors - genetics</subject><subject>Serine-Arginine Splicing Factors - metabolism</subject><subject>transcription factor</subject><subject>Transcription, Genetic - physiology</subject><subject>Ubiquitin-Protein Ligases - biosynthesis</subject><subject>Ubiquitin-Protein Ligases - genetics</subject><subject>Vero Cells</subject><subject>Virus Replication - physiology</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kcFu1DAQhi0EokvhzA35yCW7dmLHzgVpVdEWqQXUXRA3y3YmW1eJk9rJin0c3hRHW1ZwwLI0Y_ub36P5EXpLyZISwVYPxi5vKS2XgheElc_QghJZZAWnP56jBSE5zaqcyzP0KsYHkhar6Et0lgshWdoL9GsDwXlYrcPO-ZRkwdl7vBlaZ53f4Uttxz7gHN_29dTqESK-hjCksHHd0MJP_N2FKeLtYQBM8R3MhXp0vcd7p9N5N1fNSonTLb4CD3gbtI82uGHm0uXajm7vxgPWvsZfA2Td3ef1qYfX6EWj2whvnuI5-nb5cXtxnd18ufp0sb7JLGPFmFFT1prlZW4sl1xSYwQ0dUkrSWUJrDKMcq4LYrhujBV1I4iwUkqW5pALaopz9OGoO0ymg9qCH1PHagiu0-Ggeu3Uvy_e3atdv1ec8opVVRJ4_yQQ-scJ4qg6Fy20rfbQT1FRWfBSlJSLhK6OqA19jAGa0zeUqNlYlYxVs7HqaGyqePd3dyf-j5MJqI4ApBntHQQVrQNvoXYB7Kjq3v1X_DcokLVR</recordid><startdate>20161216</startdate><enddate>20161216</enddate><creator>Wang, Ziqiang</creator><creator>Liu, Qing</creator><creator>Lu, Jinhua</creator><creator>Fan, Ping</creator><creator>Xie, Weidong</creator><creator>Qiu, Wei</creator><creator>Wang, Fan</creator><creator>Hu, Guangnan</creator><creator>Zhang, Yaou</creator><general>Elsevier Inc</general><general>American Society for Biochemistry and Molecular Biology</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20161216</creationdate><title>Serine/Arginine-rich Splicing Factor 2 Modulates Herpes Simplex Virus Type 1 Replication via Regulating Viral Gene Transcriptional Activity and Pre-mRNA Splicing</title><author>Wang, Ziqiang ; Liu, Qing ; Lu, Jinhua ; Fan, Ping ; Xie, Weidong ; Qiu, Wei ; Wang, Fan ; Hu, Guangnan ; Zhang, Yaou</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c443t-1b6da4262bc58581bb7efd6198186e49b4155a30b5afbc7df707c8884478271b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Chlorocebus aethiops</topic><topic>gene expression</topic><topic>Gene Expression Regulation, Viral - physiology</topic><topic>HeLa Cells</topic><topic>herpesvirus</topic><topic>Herpesvirus 1, Human - physiology</topic><topic>host-pathogen interaction</topic><topic>Humans</topic><topic>Immediate-Early Proteins - biosynthesis</topic><topic>Immediate-Early Proteins - genetics</topic><topic>Microbiology</topic><topic>RNA Precursors - biosynthesis</topic><topic>RNA Precursors - genetics</topic><topic>RNA splicing</topic><topic>RNA Splicing - physiology</topic><topic>RNA, Viral - biosynthesis</topic><topic>RNA, Viral - genetics</topic><topic>Serine-Arginine Splicing Factors - genetics</topic><topic>Serine-Arginine Splicing Factors - metabolism</topic><topic>transcription factor</topic><topic>Transcription, Genetic - physiology</topic><topic>Ubiquitin-Protein Ligases - biosynthesis</topic><topic>Ubiquitin-Protein Ligases - genetics</topic><topic>Vero Cells</topic><topic>Virus Replication - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Ziqiang</creatorcontrib><creatorcontrib>Liu, Qing</creatorcontrib><creatorcontrib>Lu, Jinhua</creatorcontrib><creatorcontrib>Fan, Ping</creatorcontrib><creatorcontrib>Xie, Weidong</creatorcontrib><creatorcontrib>Qiu, Wei</creatorcontrib><creatorcontrib>Wang, Fan</creatorcontrib><creatorcontrib>Hu, Guangnan</creatorcontrib><creatorcontrib>Zhang, Yaou</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Ziqiang</au><au>Liu, Qing</au><au>Lu, Jinhua</au><au>Fan, Ping</au><au>Xie, Weidong</au><au>Qiu, Wei</au><au>Wang, Fan</au><au>Hu, Guangnan</au><au>Zhang, Yaou</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Serine/Arginine-rich Splicing Factor 2 Modulates Herpes Simplex Virus Type 1 Replication via Regulating Viral Gene Transcriptional Activity and Pre-mRNA Splicing</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>2016-12-16</date><risdate>2016</risdate><volume>291</volume><issue>51</issue><spage>26377</spage><epage>26387</epage><pages>26377-26387</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>Once it enters the host cell, herpes simplex virus type 1 (HSV-1) recruits a series of host cell factors to facilitate its life cycle. Here, we demonstrate that serine/arginine-rich splicing factor 2 (SRSF2), which is an important component of the splicing speckle, mediates HSV-1 replication by regulating viral gene expression at the transcriptional and posttranscriptional levels. Our results indicate that SRSF2 functions as a transcriptional activator by directly binding to infected cell polypeptide 0 (ICP0), infected cell polypeptide 27 (ICP27), and thymidine kinase promoters. Moreover, SRSF2 participates in ICP0 pre-mRNA splicing by recognizing binding sites in ICP0 exon 3. These findings provide insight into the functions of SRSF2 in HSV-1 replication and gene expression.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>27784784</pmid><doi>10.1074/jbc.M116.753046</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Chlorocebus aethiops gene expression Gene Expression Regulation, Viral - physiology HeLa Cells herpesvirus Herpesvirus 1, Human - physiology host-pathogen interaction Humans Immediate-Early Proteins - biosynthesis Immediate-Early Proteins - genetics Microbiology RNA Precursors - biosynthesis RNA Precursors - genetics RNA splicing RNA Splicing - physiology RNA, Viral - biosynthesis RNA, Viral - genetics Serine-Arginine Splicing Factors - genetics Serine-Arginine Splicing Factors - metabolism transcription factor Transcription, Genetic - physiology Ubiquitin-Protein Ligases - biosynthesis Ubiquitin-Protein Ligases - genetics Vero Cells Virus Replication - physiology |
| title | Serine/Arginine-rich Splicing Factor 2 Modulates Herpes Simplex Virus Type 1 Replication via Regulating Viral Gene Transcriptional Activity and Pre-mRNA Splicing |
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