Validated methodology for quantifying infestation levels of dreissenid mussels in environmental DNA (eDNA) samples
The zebra mussel ( Dreissena polymorpha Pallas, 1771) and the quagga mussel ( D. rostriformis Deshayes, 1838) are successful invasive bivalves with substantial ecological and economic impacts in freshwater systems once they become established. Since their eradication is extremely difficult, their de...
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| Veröffentlicht in: | Scientific reports 2016-12, Vol.6 (1), p.39067-39067, Article 39067 |
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| creator | Peñarrubia, Luis Alcaraz, Carles Vaate, Abraham bij de Sanz, Nuria Pla, Carles Vidal, Oriol Viñas, Jordi |
| description | The zebra mussel (
Dreissena polymorpha
Pallas, 1771) and the quagga mussel (
D. rostriformis
Deshayes, 1838) are successful invasive bivalves with substantial ecological and economic impacts in freshwater systems once they become established. Since their eradication is extremely difficult, their detection at an early stage is crucial to prevent spread. In this study, we optimized and validated a qPCR detection method based on the histone H2B gene to quantify combined infestation levels of zebra and quagga mussels in environmental DNA samples. Our results show specific dreissenid DNA present in filtered water samples for which microscopic diagnostic identification for larvae failed. Monitoring a large number of locations for invasive dreissenid species based on a highly specific environmental DNA qPCR assay may prove to be an essential tool for management and control plans focused on prevention of establishment of dreissenid mussels in new locations. |
| doi_str_mv | 10.1038/srep39067 |
| format | Article |
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Dreissena polymorpha
Pallas, 1771) and the quagga mussel (
D. rostriformis
Deshayes, 1838) are successful invasive bivalves with substantial ecological and economic impacts in freshwater systems once they become established. Since their eradication is extremely difficult, their detection at an early stage is crucial to prevent spread. In this study, we optimized and validated a qPCR detection method based on the histone H2B gene to quantify combined infestation levels of zebra and quagga mussels in environmental DNA samples. Our results show specific dreissenid DNA present in filtered water samples for which microscopic diagnostic identification for larvae failed. Monitoring a large number of locations for invasive dreissenid species based on a highly specific environmental DNA qPCR assay may prove to be an essential tool for management and control plans focused on prevention of establishment of dreissenid mussels in new locations.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep39067</identifier><identifier>PMID: 27966602</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>45/23 ; 45/77 ; 631/158/2464 ; 631/208 ; Animals ; Animals invasors ; Biological invasions ; Deoxyribonucleic acid ; DNA ; DNA - genetics ; Dreissena - genetics ; Dreissena - growth & development ; Economic impact ; Environmental DNA ; Environmental Monitoring - methods ; Fresh Water - chemistry ; Fresh Water - parasitology ; Freshwater mussels ; Genetic testing ; Genetics ; Genètica ; Genètica de poblacions ; Histone H2B ; Histones - genetics ; Humanities and Social Sciences ; Infestation ; Introduced organisms ; Introduced Species ; Invasions biològiques ; Invasive species ; Larva - genetics ; Larva - growth & development ; Larvae ; Mollusks ; multidisciplinary ; Musclos d’aigua dolça ; Population genetics ; Science ; Water analysis ; Water Purification ; Water sampling</subject><ispartof>Scientific reports, 2016-12, Vol.6 (1), p.39067-39067, Article 39067</ispartof><rights>The Author(s) 2016</rights><rights>Copyright Nature Publishing Group Dec 2016</rights><rights>Attribution 4.0 Spain info:eu-repo/semantics/openAccess <a href="http://creativecommons.org/licenses/by/4.0/es/">http://creativecommons.org/licenses/by/4.0/es/</a></rights><rights>Copyright © 2016, The Author(s) 2016 The Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c480t-b2706d72682b41ceb1a59c382195151b6e44165c10af3688392ea7c5818c25043</citedby><cites>FETCH-LOGICAL-c480t-b2706d72682b41ceb1a59c382195151b6e44165c10af3688392ea7c5818c25043</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5155230/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5155230/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,26951,27901,27902,41096,42165,51551,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27966602$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Peñarrubia, Luis</creatorcontrib><creatorcontrib>Alcaraz, Carles</creatorcontrib><creatorcontrib>Vaate, Abraham bij de</creatorcontrib><creatorcontrib>Sanz, Nuria</creatorcontrib><creatorcontrib>Pla, Carles</creatorcontrib><creatorcontrib>Vidal, Oriol</creatorcontrib><creatorcontrib>Viñas, Jordi</creatorcontrib><title>Validated methodology for quantifying infestation levels of dreissenid mussels in environmental DNA (eDNA) samples</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>The zebra mussel (
Dreissena polymorpha
Pallas, 1771) and the quagga mussel (
D. rostriformis
Deshayes, 1838) are successful invasive bivalves with substantial ecological and economic impacts in freshwater systems once they become established. Since their eradication is extremely difficult, their detection at an early stage is crucial to prevent spread. In this study, we optimized and validated a qPCR detection method based on the histone H2B gene to quantify combined infestation levels of zebra and quagga mussels in environmental DNA samples. Our results show specific dreissenid DNA present in filtered water samples for which microscopic diagnostic identification for larvae failed. Monitoring a large number of locations for invasive dreissenid species based on a highly specific environmental DNA qPCR assay may prove to be an essential tool for management and control plans focused on prevention of establishment of dreissenid mussels in new locations.</description><subject>45/23</subject><subject>45/77</subject><subject>631/158/2464</subject><subject>631/208</subject><subject>Animals</subject><subject>Animals invasors</subject><subject>Biological invasions</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA - genetics</subject><subject>Dreissena - genetics</subject><subject>Dreissena - growth & development</subject><subject>Economic impact</subject><subject>Environmental DNA</subject><subject>Environmental Monitoring - methods</subject><subject>Fresh Water - chemistry</subject><subject>Fresh Water - parasitology</subject><subject>Freshwater mussels</subject><subject>Genetic testing</subject><subject>Genetics</subject><subject>Genètica</subject><subject>Genètica de poblacions</subject><subject>Histone H2B</subject><subject>Histones - 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Academic</collection><collection>Recercat</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Peñarrubia, Luis</au><au>Alcaraz, Carles</au><au>Vaate, Abraham bij de</au><au>Sanz, Nuria</au><au>Pla, Carles</au><au>Vidal, Oriol</au><au>Viñas, Jordi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Validated methodology for quantifying infestation levels of dreissenid mussels in environmental DNA (eDNA) samples</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2016-12-14</date><risdate>2016</risdate><volume>6</volume><issue>1</issue><spage>39067</spage><epage>39067</epage><pages>39067-39067</pages><artnum>39067</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>The zebra mussel (
Dreissena polymorpha
Pallas, 1771) and the quagga mussel (
D. rostriformis
Deshayes, 1838) are successful invasive bivalves with substantial ecological and economic impacts in freshwater systems once they become established. Since their eradication is extremely difficult, their detection at an early stage is crucial to prevent spread. In this study, we optimized and validated a qPCR detection method based on the histone H2B gene to quantify combined infestation levels of zebra and quagga mussels in environmental DNA samples. Our results show specific dreissenid DNA present in filtered water samples for which microscopic diagnostic identification for larvae failed. Monitoring a large number of locations for invasive dreissenid species based on a highly specific environmental DNA qPCR assay may prove to be an essential tool for management and control plans focused on prevention of establishment of dreissenid mussels in new locations.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>27966602</pmid><doi>10.1038/srep39067</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 45/23 45/77 631/158/2464 631/208 Animals Animals invasors Biological invasions Deoxyribonucleic acid DNA DNA - genetics Dreissena - genetics Dreissena - growth & development Economic impact Environmental DNA Environmental Monitoring - methods Fresh Water - chemistry Fresh Water - parasitology Freshwater mussels Genetic testing Genetics Genètica Genètica de poblacions Histone H2B Histones - genetics Humanities and Social Sciences Infestation Introduced organisms Introduced Species Invasions biològiques Invasive species Larva - genetics Larva - growth & development Larvae Mollusks multidisciplinary Musclos d’aigua dolça Population genetics Science Water analysis Water Purification Water sampling |
| title | Validated methodology for quantifying infestation levels of dreissenid mussels in environmental DNA (eDNA) samples |
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