Nanoliter-Scale Protein Crystallization and Screening with a Microfluidic Droplet Robot
Large-scale screening of hundreds or even thousands of crystallization conditions while with low sample consumption is in urgent need, in current structural biology research. Here we describe a fully-automated droplet robot for nanoliter-scale crystallization screening that combines the advantages o...
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| Veröffentlicht in: | Scientific reports 2014-05, Vol.4 (1), p.5046-5046, Article 5046 |
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| creator | Zhu, Ying Zhu, Li-Na Guo, Rui Cui, Heng-Jun Ye, Sheng Fang, Qun |
| description | Large-scale screening of hundreds or even thousands of crystallization conditions while with low sample consumption is in urgent need, in current structural biology research. Here we describe a fully-automated droplet robot for nanoliter-scale crystallization screening that combines the advantages of both automated robotics technique for protein crystallization screening and the droplet-based microfluidic technique. A semi-contact dispensing method was developed to achieve flexible, programmable and reliable liquid-handling operations for nanoliter-scale protein crystallization experiments. We applied the droplet robot in large-scale screening of crystallization conditions of five soluble proteins and one membrane protein with 35–96 different crystallization conditions, study of volume effects on protein crystallization and determination of phase diagrams of two proteins. The volume for each droplet reactor is only
ca
. 4–8 nL. The protein consumption significantly reduces 50–500 fold compared with current crystallization stations. |
| doi_str_mv | 10.1038/srep05046 |
| format | Article |
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Zhu, Li-Na ; Guo, Rui ; Cui, Heng-Jun ; Ye, Sheng ; Fang, Qun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-83f466411a280285a0de652c64e4fa2535dedeac18e775a81ff8d685050030723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>49</topic><topic>631/1647/2163</topic><topic>631/1647/2196/2197</topic><topic>631/1647/277</topic><topic>82/56</topic><topic>82/62</topic><topic>Automation</topic><topic>Crystallization</topic><topic>Crystallization - methods</topic><topic>Humanities and Social Sciences</topic><topic>Humans</topic><topic>Membrane proteins</topic><topic>Membrane Proteins - chemistry</topic><topic>Microfluidic Analytical Techniques - instrumentation</topic><topic>Microfluidic Analytical Techniques - methods</topic><topic>Microfluidics</topic><topic>Models, Molecular</topic><topic>multidisciplinary</topic><topic>Phase diagrams</topic><topic>Protein Conformation</topic><topic>Protein folding</topic><topic>Proteins</topic><topic>Robotics</topic><topic>Robots</topic><topic>Science</topic><topic>X-Ray Diffraction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhu, Ying</creatorcontrib><creatorcontrib>Zhu, Li-Na</creatorcontrib><creatorcontrib>Guo, Rui</creatorcontrib><creatorcontrib>Cui, Heng-Jun</creatorcontrib><creatorcontrib>Ye, Sheng</creatorcontrib><creatorcontrib>Fang, Qun</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhu, Ying</au><au>Zhu, Li-Na</au><au>Guo, Rui</au><au>Cui, Heng-Jun</au><au>Ye, Sheng</au><au>Fang, Qun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nanoliter-Scale Protein Crystallization and Screening with a Microfluidic Droplet Robot</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2014-05-23</date><risdate>2014</risdate><volume>4</volume><issue>1</issue><spage>5046</spage><epage>5046</epage><pages>5046-5046</pages><artnum>5046</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Large-scale screening of hundreds or even thousands of crystallization conditions while with low sample consumption is in urgent need, in current structural biology research. Here we describe a fully-automated droplet robot for nanoliter-scale crystallization screening that combines the advantages of both automated robotics technique for protein crystallization screening and the droplet-based microfluidic technique. A semi-contact dispensing method was developed to achieve flexible, programmable and reliable liquid-handling operations for nanoliter-scale protein crystallization experiments. We applied the droplet robot in large-scale screening of crystallization conditions of five soluble proteins and one membrane protein with 35–96 different crystallization conditions, study of volume effects on protein crystallization and determination of phase diagrams of two proteins. The volume for each droplet reactor is only
ca
. 4–8 nL. The protein consumption significantly reduces 50–500 fold compared with current crystallization stations.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>24854085</pmid><doi>10.1038/srep05046</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 49 631/1647/2163 631/1647/2196/2197 631/1647/277 82/56 82/62 Automation Crystallization Crystallization - methods Humanities and Social Sciences Humans Membrane proteins Membrane Proteins - chemistry Microfluidic Analytical Techniques - instrumentation Microfluidic Analytical Techniques - methods Microfluidics Models, Molecular multidisciplinary Phase diagrams Protein Conformation Protein folding Proteins Robotics Robots Science X-Ray Diffraction |
| title | Nanoliter-Scale Protein Crystallization and Screening with a Microfluidic Droplet Robot |
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