Overexpression of Hsp25 in K1735 murine melanoma cells enhances susceptibility to natural killer cytotoxicity
In the present study we used a murine melanoma model to investigate the effect of the 25-kDa heat shock protein (Hsp25) on natural killer (NK) cytotoxicity. The melanoma lines K1735-Cl23 (low metastatic potential) and K1735-M2 (high metastatic potential) were transfected with hsp25 and a control pla...
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| Veröffentlicht in: | Cell stress & chaperones 2002-01, Vol.7 (1), p.107-117 |
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| creator | Jantschitsch, Christian Trautinger, Franz Klosner, Gabriele Gsur, Andrea Herbacek, Irene Micksche, Michael Kindås-Mügge, Ingela |
| description | In the present study we used a murine melanoma model to investigate the effect of the 25-kDa heat shock protein (Hsp25) on natural killer (NK) cytotoxicity. The melanoma lines K1735-Cl23 (low metastatic potential) and K1735-M2 (high metastatic potential) were transfected with hsp25 and a control plasmid. Highly purified interleukin (IL)-2–stimulated DX-5+ NK cells showed enhanced lysis of Hsp25-overexpressing K1735-Cl23 targets in comparison with controls. In contrast, there was no difference in susceptibility to lysis by purified IL-2–stimulated DX-5+ NK cells between Hsp25-overexpressing and control-transfected K1735-M2 targets. Fluorescence-activated cell sorter analysis revealed that Hsp25 is displayed on the cell surface independently of Hsp25 overexpression and metastatic phenotype. Thus, surface localization of Hsp25 does not correlate with the target cell susceptibility to killing. To sum up, a cytoplasmic overexpression of Hsp25 is associated with an increased susceptibility to lysis by DX-5+ NK cells in the low-metastatic murine melanoma model investigated. |
| doi_str_mv | 10.1379/1466-1268(2002)007<0107:OOHIKM>2.0.CO;2 |
| format | Article |
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The melanoma lines K1735-Cl23 (low metastatic potential) and K1735-M2 (high metastatic potential) were transfected with hsp25 and a control plasmid. Highly purified interleukin (IL)-2–stimulated DX-5+ NK cells showed enhanced lysis of Hsp25-overexpressing K1735-Cl23 targets in comparison with controls. In contrast, there was no difference in susceptibility to lysis by purified IL-2–stimulated DX-5+ NK cells between Hsp25-overexpressing and control-transfected K1735-M2 targets. Fluorescence-activated cell sorter analysis revealed that Hsp25 is displayed on the cell surface independently of Hsp25 overexpression and metastatic phenotype. Thus, surface localization of Hsp25 does not correlate with the target cell susceptibility to killing. To sum up, a cytoplasmic overexpression of Hsp25 is associated with an increased susceptibility to lysis by DX-5+ NK cells in the low-metastatic murine melanoma model investigated.</description><identifier>ISSN: 1355-8145</identifier><identifier>EISSN: 1466-1268</identifier><identifier>DOI: 10.1379/1466-1268(2002)007<0107:OOHIKM>2.0.CO;2</identifier><identifier>PMID: 11892982</identifier><language>eng</language><publisher>Netherlands: Cell Stress Society International</publisher><subject>Animals ; B lymphocytes ; Cell lines ; Cytotoxicity ; Cytotoxicity Tests, Immunologic ; Flow Cytometry ; Gene Expression Regulation, Neoplastic - immunology ; Heat shock proteins ; Histocompatibility Antigens Class II - metabolism ; Killer Cells, Natural - immunology ; Lymphocytes ; Melanoma ; Mice ; Mice, Inbred C3H ; Natural killer cells ; Neoplasm Metastasis - immunology ; Neoplasm Proteins - genetics ; Neoplasm Proteins - immunology ; Original ; Original s ; Tumor cell line ; Tumor Cells, Cultured - immunology ; Tumors</subject><ispartof>Cell stress & chaperones, 2002-01, Vol.7 (1), p.107-117</ispartof><rights>Cell Stress Society International</rights><rights>Copyright 2001 Cell Stress Society International</rights><rights>Copyright © 2002, Cell Stress Society International 2002</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-b473t-41182a30e7d18969586809bdfebe09e014c5f4622480f7c7027b826e9edef9233</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://bioone.org/doi/pdf/10.1379/1466-1268(2002)007<0107:OOHIKM>2.0.CO;2$$EPDF$$P50$$Gbioone$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/1601903$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,315,728,781,785,804,886,4025,26980,27925,27926,27927,52365,53793,53795,58019,58252</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11892982$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jantschitsch, Christian</creatorcontrib><creatorcontrib>Trautinger, Franz</creatorcontrib><creatorcontrib>Klosner, Gabriele</creatorcontrib><creatorcontrib>Gsur, Andrea</creatorcontrib><creatorcontrib>Herbacek, Irene</creatorcontrib><creatorcontrib>Micksche, Michael</creatorcontrib><creatorcontrib>Kindås-Mügge, Ingela</creatorcontrib><title>Overexpression of Hsp25 in K1735 murine melanoma cells enhances susceptibility to natural killer cytotoxicity</title><title>Cell stress & chaperones</title><addtitle>Cell Stress Chaperones</addtitle><description>In the present study we used a murine melanoma model to investigate the effect of the 25-kDa heat shock protein (Hsp25) on natural killer (NK) cytotoxicity. The melanoma lines K1735-Cl23 (low metastatic potential) and K1735-M2 (high metastatic potential) were transfected with hsp25 and a control plasmid. Highly purified interleukin (IL)-2–stimulated DX-5+ NK cells showed enhanced lysis of Hsp25-overexpressing K1735-Cl23 targets in comparison with controls. In contrast, there was no difference in susceptibility to lysis by purified IL-2–stimulated DX-5+ NK cells between Hsp25-overexpressing and control-transfected K1735-M2 targets. Fluorescence-activated cell sorter analysis revealed that Hsp25 is displayed on the cell surface independently of Hsp25 overexpression and metastatic phenotype. Thus, surface localization of Hsp25 does not correlate with the target cell susceptibility to killing. To sum up, a cytoplasmic overexpression of Hsp25 is associated with an increased susceptibility to lysis by DX-5+ NK cells in the low-metastatic murine melanoma model investigated.</description><subject>Animals</subject><subject>B lymphocytes</subject><subject>Cell lines</subject><subject>Cytotoxicity</subject><subject>Cytotoxicity Tests, Immunologic</subject><subject>Flow Cytometry</subject><subject>Gene Expression Regulation, Neoplastic - immunology</subject><subject>Heat shock proteins</subject><subject>Histocompatibility Antigens Class II - metabolism</subject><subject>Killer Cells, Natural - immunology</subject><subject>Lymphocytes</subject><subject>Melanoma</subject><subject>Mice</subject><subject>Mice, Inbred C3H</subject><subject>Natural killer cells</subject><subject>Neoplasm Metastasis - immunology</subject><subject>Neoplasm Proteins - genetics</subject><subject>Neoplasm Proteins - immunology</subject><subject>Original</subject><subject>Original s</subject><subject>Tumor cell line</subject><subject>Tumor Cells, Cultured - immunology</subject><subject>Tumors</subject><issn>1355-8145</issn><issn>1466-1268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqdUV9v1SAcJUbj5rZvYAyP-tA7oC0UnSbmZu4um-mLeyaU-6tjttAAd9n99tJ02fR1TxDOnxzOQeiUkhUthTylFecFZbz5yAhhnwgRZ4QS8bltN5dXP7-xFVmt2y_sFTp8Yr7O97Kui4ZW9QF6F-MdyTIh6Ft0QGkjmWzYIRrbewjwMAWI0XqHfY83cWI1tg5fUVHWeNwF6wCPMGjnR40NDEPE4G61MxBx3EUDU7KdHWza4-Sx02kX9ID_2GGAgM0--eQfrMnwMXrT6yHCyeN5hG5-nP9ab4rr9uJy_f266CpRpqLK-ZguCYhtDspl3fCGyG7bQwdEAqGVqfuKM1Y1pBdGECa6hnGQsIVesrI8Ql8X32nXjbA14FJOpKZgRx32ymur_kecvVW__b2qaSUkz_qLRW-CjzFA_ySlRM2DqLlmNdes5kFUblbNg6hlEJVf1LpVLDt9-DfJs8_jApnwfiHcxeTDM84JlWT-yfkCd9Z7By_O8RfcEap1</recordid><startdate>200201</startdate><enddate>200201</enddate><creator>Jantschitsch, Christian</creator><creator>Trautinger, Franz</creator><creator>Klosner, Gabriele</creator><creator>Gsur, Andrea</creator><creator>Herbacek, Irene</creator><creator>Micksche, Michael</creator><creator>Kindås-Mügge, Ingela</creator><general>Cell Stress Society International</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>200201</creationdate><title>Overexpression of Hsp25 in K1735 murine melanoma cells enhances susceptibility to natural killer cytotoxicity</title><author>Jantschitsch, Christian ; Trautinger, Franz ; Klosner, Gabriele ; Gsur, Andrea ; Herbacek, Irene ; Micksche, Michael ; Kindås-Mügge, Ingela</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b473t-41182a30e7d18969586809bdfebe09e014c5f4622480f7c7027b826e9edef9233</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>B lymphocytes</topic><topic>Cell lines</topic><topic>Cytotoxicity</topic><topic>Cytotoxicity Tests, Immunologic</topic><topic>Flow Cytometry</topic><topic>Gene Expression Regulation, Neoplastic - immunology</topic><topic>Heat shock proteins</topic><topic>Histocompatibility Antigens Class II - metabolism</topic><topic>Killer Cells, Natural - immunology</topic><topic>Lymphocytes</topic><topic>Melanoma</topic><topic>Mice</topic><topic>Mice, Inbred C3H</topic><topic>Natural killer cells</topic><topic>Neoplasm Metastasis - immunology</topic><topic>Neoplasm Proteins - genetics</topic><topic>Neoplasm Proteins - immunology</topic><topic>Original</topic><topic>Original s</topic><topic>Tumor cell line</topic><topic>Tumor Cells, Cultured - immunology</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jantschitsch, Christian</creatorcontrib><creatorcontrib>Trautinger, Franz</creatorcontrib><creatorcontrib>Klosner, Gabriele</creatorcontrib><creatorcontrib>Gsur, Andrea</creatorcontrib><creatorcontrib>Herbacek, Irene</creatorcontrib><creatorcontrib>Micksche, Michael</creatorcontrib><creatorcontrib>Kindås-Mügge, Ingela</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cell stress & chaperones</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jantschitsch, Christian</au><au>Trautinger, Franz</au><au>Klosner, Gabriele</au><au>Gsur, Andrea</au><au>Herbacek, Irene</au><au>Micksche, Michael</au><au>Kindås-Mügge, Ingela</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Overexpression of Hsp25 in K1735 murine melanoma cells enhances susceptibility to natural killer cytotoxicity</atitle><jtitle>Cell stress & chaperones</jtitle><addtitle>Cell Stress Chaperones</addtitle><date>2002-01</date><risdate>2002</risdate><volume>7</volume><issue>1</issue><spage>107</spage><epage>117</epage><pages>107-117</pages><issn>1355-8145</issn><eissn>1466-1268</eissn><abstract>In the present study we used a murine melanoma model to investigate the effect of the 25-kDa heat shock protein (Hsp25) on natural killer (NK) cytotoxicity. The melanoma lines K1735-Cl23 (low metastatic potential) and K1735-M2 (high metastatic potential) were transfected with hsp25 and a control plasmid. Highly purified interleukin (IL)-2–stimulated DX-5+ NK cells showed enhanced lysis of Hsp25-overexpressing K1735-Cl23 targets in comparison with controls. In contrast, there was no difference in susceptibility to lysis by purified IL-2–stimulated DX-5+ NK cells between Hsp25-overexpressing and control-transfected K1735-M2 targets. Fluorescence-activated cell sorter analysis revealed that Hsp25 is displayed on the cell surface independently of Hsp25 overexpression and metastatic phenotype. Thus, surface localization of Hsp25 does not correlate with the target cell susceptibility to killing. 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| subjects | Animals B lymphocytes Cell lines Cytotoxicity Cytotoxicity Tests, Immunologic Flow Cytometry Gene Expression Regulation, Neoplastic - immunology Heat shock proteins Histocompatibility Antigens Class II - metabolism Killer Cells, Natural - immunology Lymphocytes Melanoma Mice Mice, Inbred C3H Natural killer cells Neoplasm Metastasis - immunology Neoplasm Proteins - genetics Neoplasm Proteins - immunology Original Original s Tumor cell line Tumor Cells, Cultured - immunology Tumors |
| title | Overexpression of Hsp25 in K1735 murine melanoma cells enhances susceptibility to natural killer cytotoxicity |
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