Cellular and molecular characterization of a stem rust resistance locus on wheat chromosome 7AL
Wheat stem rust, caused by Puccinia graminis f. sp. tritici, is a major wheat disease which is mainly controlled through the release of resistant cultivars containing one or several resistance genes. Considerable effort has been put into the discovery of new resistance genes, but knowledge of their...
Gespeichert in:
| Veröffentlicht in: | BMC research notes 2016-12, Vol.9 (1), p.502-502, Article 502 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 502 |
|---|---|
| container_issue | 1 |
| container_start_page | 502 |
| container_title | BMC research notes |
| container_volume | 9 |
| creator | Pujol, Vincent Robles, Jose Wang, Penghao Taylor, Jen Zhang, Peng Huang, Li Tabe, Linda Lagudah, Evans |
| description | Wheat stem rust, caused by Puccinia graminis f. sp. tritici, is a major wheat disease which is mainly controlled through the release of resistant cultivars containing one or several resistance genes. Considerable effort has been put into the discovery of new resistance genes, but knowledge of their mechanisms of action is often lacking. In this study, the mechanism of resistance conferred by a recently discovered stem rust resistance locus on wheat chromosome 7AL was investigated through microscopic observations and RNA-sequencing, using the susceptible line Columbus and the independent, backcrossed, resistant lines Columbus-NS765 and Columbus-NS766.
Microscopic observations of infected leaves revealed that the resistance conferred by the 7AL resistance locus was initiated 2 days post-inoculation, upon the fungus entry into the plant through the stoma. Resistance was manifested by death of guard and epidermal cells adjacent to an infection site. Occasionally, similar observations were made in the susceptible line, suggesting that the resistance response was the same in all genotypes, but enhanced in the resistant lines. Transcriptomic analysis, combined with assignment of genes to wheat chromosomes, revealed a disproportionately high number of differentially expressed genes were located on chromosomes 7AL and 6A. A number of genes annotated as cysteine-rich receptor-like kinases were located on chromosome 7AL. Closer investigation indicated that the encoded proteins were in fact putative receptor-like cytoplasmic kinases. One of the putative RLCK genes contained a SNP marker previously shown to co-segregate with the 7AL resistance locus. The results also indicated the presence of a large introgression on chromosome 6A in both resistant lines, but whether it has any role in the resistance response is unclear.
This study represents the first investigation on the resistance mechanism conferred by the wheat 7AL stem rust resistance locus. The resistance response was associated with pre-haustorial cell death, and the transcriptome analysis suggested putative receptor-like cytoplasmic kinases as candidate resistance genes for further investigation. |
| doi_str_mv | 10.1186/s13104-016-2320-z |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5143453</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A473181590</galeid><sourcerecordid>A473181590</sourcerecordid><originalsourceid>FETCH-LOGICAL-c443z-a49c85bcd9b6d8977ab9bccee92f9cd1183295dab53a340e1b9a6ea8473d85413</originalsourceid><addsrcrecordid>eNptkktv1DAUhSMEoqXwA9ggS2xgkeJnYm-QRiMelUbqBthaN87NjKskLrbDY349LlNKByEv_PrOufbVqarnjJ4zpps3iQlGZU1ZU3PBab1_UJ2yVjU1VZQ-vLc-qZ6kdEVpw7Rmj6sT3hrecq5PK7vGcVxGiATmnkxhRPd753YQwWWMfg_Zh5mEgQBJGScSl5RJxORThtkhGYNbEinI9x1CLsoYppDChKRdbZ5WjwYYEz67nc-qz-_ffVp_rDeXHy7Wq03tpBT7GqRxWnWuN13Ta9O20JnOOUTDB-P68lvBjeqhUwKEpMg6Aw2Clq3otZJMnFVvD77XSzdh73DOEUZ7Hf0E8acN4O3xzex3dhu-WcWkkEoUg1e3BjF8XTBlO_nkSnNgxrAky0otrTnlsqAv_0GvwhLn8r1CKakFY6b9S21hROvnIZS67sbUrsqzmWbK0EKd_4cqo8fJuzDj4Mv5keD1kaAwGX_kLSwp2YvLL8csO7AuhpQiDnf9YNTeJMgeEmRLguxNguy-aF7cb-Sd4k9kxC8UKcDb</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1854831197</pqid></control><display><type>article</type><title>Cellular and molecular characterization of a stem rust resistance locus on wheat chromosome 7AL</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><source>DOAJ Directory of Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>PubMed Central Open Access</source><source>Springer Nature OA Free Journals</source><creator>Pujol, Vincent ; Robles, Jose ; Wang, Penghao ; Taylor, Jen ; Zhang, Peng ; Huang, Li ; Tabe, Linda ; Lagudah, Evans</creator><creatorcontrib>Pujol, Vincent ; Robles, Jose ; Wang, Penghao ; Taylor, Jen ; Zhang, Peng ; Huang, Li ; Tabe, Linda ; Lagudah, Evans</creatorcontrib><description>Wheat stem rust, caused by Puccinia graminis f. sp. tritici, is a major wheat disease which is mainly controlled through the release of resistant cultivars containing one or several resistance genes. Considerable effort has been put into the discovery of new resistance genes, but knowledge of their mechanisms of action is often lacking. In this study, the mechanism of resistance conferred by a recently discovered stem rust resistance locus on wheat chromosome 7AL was investigated through microscopic observations and RNA-sequencing, using the susceptible line Columbus and the independent, backcrossed, resistant lines Columbus-NS765 and Columbus-NS766.
Microscopic observations of infected leaves revealed that the resistance conferred by the 7AL resistance locus was initiated 2 days post-inoculation, upon the fungus entry into the plant through the stoma. Resistance was manifested by death of guard and epidermal cells adjacent to an infection site. Occasionally, similar observations were made in the susceptible line, suggesting that the resistance response was the same in all genotypes, but enhanced in the resistant lines. Transcriptomic analysis, combined with assignment of genes to wheat chromosomes, revealed a disproportionately high number of differentially expressed genes were located on chromosomes 7AL and 6A. A number of genes annotated as cysteine-rich receptor-like kinases were located on chromosome 7AL. Closer investigation indicated that the encoded proteins were in fact putative receptor-like cytoplasmic kinases. One of the putative RLCK genes contained a SNP marker previously shown to co-segregate with the 7AL resistance locus. The results also indicated the presence of a large introgression on chromosome 6A in both resistant lines, but whether it has any role in the resistance response is unclear.
This study represents the first investigation on the resistance mechanism conferred by the wheat 7AL stem rust resistance locus. The resistance response was associated with pre-haustorial cell death, and the transcriptome analysis suggested putative receptor-like cytoplasmic kinases as candidate resistance genes for further investigation.</description><identifier>ISSN: 1756-0500</identifier><identifier>EISSN: 1756-0500</identifier><identifier>DOI: 10.1186/s13104-016-2320-z</identifier><identifier>PMID: 27927228</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Basidiomycota ; Chromosome Mapping ; Chromosomes ; Chromosomes, Plant - genetics ; Cyclopentanes - chemistry ; Disease resistance (Plants) ; Disease Resistance - genetics ; Fluorescein-5-isothiocyanate ; Genes, Plant ; Genetic aspects ; Oxylipins - chemistry ; Phenotype ; Physiological aspects ; Plant Diseases - genetics ; Plant Diseases - microbiology ; Polymorphism, Single Nucleotide ; Salicylic Acid - chemistry ; Sequence Analysis, RNA ; Signal Transduction ; Transcriptome ; Triticum - genetics ; Triticum - microbiology ; Wheat</subject><ispartof>BMC research notes, 2016-12, Vol.9 (1), p.502-502, Article 502</ispartof><rights>COPYRIGHT 2016 BioMed Central Ltd.</rights><rights>Copyright BioMed Central 2016</rights><rights>The Author(s) 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c443z-a49c85bcd9b6d8977ab9bccee92f9cd1183295dab53a340e1b9a6ea8473d85413</citedby><cites>FETCH-LOGICAL-c443z-a49c85bcd9b6d8977ab9bccee92f9cd1183295dab53a340e1b9a6ea8473d85413</cites><orcidid>0000-0001-9903-3475</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5143453/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5143453/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27927228$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pujol, Vincent</creatorcontrib><creatorcontrib>Robles, Jose</creatorcontrib><creatorcontrib>Wang, Penghao</creatorcontrib><creatorcontrib>Taylor, Jen</creatorcontrib><creatorcontrib>Zhang, Peng</creatorcontrib><creatorcontrib>Huang, Li</creatorcontrib><creatorcontrib>Tabe, Linda</creatorcontrib><creatorcontrib>Lagudah, Evans</creatorcontrib><title>Cellular and molecular characterization of a stem rust resistance locus on wheat chromosome 7AL</title><title>BMC research notes</title><addtitle>BMC Res Notes</addtitle><description>Wheat stem rust, caused by Puccinia graminis f. sp. tritici, is a major wheat disease which is mainly controlled through the release of resistant cultivars containing one or several resistance genes. Considerable effort has been put into the discovery of new resistance genes, but knowledge of their mechanisms of action is often lacking. In this study, the mechanism of resistance conferred by a recently discovered stem rust resistance locus on wheat chromosome 7AL was investigated through microscopic observations and RNA-sequencing, using the susceptible line Columbus and the independent, backcrossed, resistant lines Columbus-NS765 and Columbus-NS766.
Microscopic observations of infected leaves revealed that the resistance conferred by the 7AL resistance locus was initiated 2 days post-inoculation, upon the fungus entry into the plant through the stoma. Resistance was manifested by death of guard and epidermal cells adjacent to an infection site. Occasionally, similar observations were made in the susceptible line, suggesting that the resistance response was the same in all genotypes, but enhanced in the resistant lines. Transcriptomic analysis, combined with assignment of genes to wheat chromosomes, revealed a disproportionately high number of differentially expressed genes were located on chromosomes 7AL and 6A. A number of genes annotated as cysteine-rich receptor-like kinases were located on chromosome 7AL. Closer investigation indicated that the encoded proteins were in fact putative receptor-like cytoplasmic kinases. One of the putative RLCK genes contained a SNP marker previously shown to co-segregate with the 7AL resistance locus. The results also indicated the presence of a large introgression on chromosome 6A in both resistant lines, but whether it has any role in the resistance response is unclear.
This study represents the first investigation on the resistance mechanism conferred by the wheat 7AL stem rust resistance locus. The resistance response was associated with pre-haustorial cell death, and the transcriptome analysis suggested putative receptor-like cytoplasmic kinases as candidate resistance genes for further investigation.</description><subject>Basidiomycota</subject><subject>Chromosome Mapping</subject><subject>Chromosomes</subject><subject>Chromosomes, Plant - genetics</subject><subject>Cyclopentanes - chemistry</subject><subject>Disease resistance (Plants)</subject><subject>Disease Resistance - genetics</subject><subject>Fluorescein-5-isothiocyanate</subject><subject>Genes, Plant</subject><subject>Genetic aspects</subject><subject>Oxylipins - chemistry</subject><subject>Phenotype</subject><subject>Physiological aspects</subject><subject>Plant Diseases - genetics</subject><subject>Plant Diseases - microbiology</subject><subject>Polymorphism, Single Nucleotide</subject><subject>Salicylic Acid - chemistry</subject><subject>Sequence Analysis, RNA</subject><subject>Signal Transduction</subject><subject>Transcriptome</subject><subject>Triticum - genetics</subject><subject>Triticum - microbiology</subject><subject>Wheat</subject><issn>1756-0500</issn><issn>1756-0500</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNptkktv1DAUhSMEoqXwA9ggS2xgkeJnYm-QRiMelUbqBthaN87NjKskLrbDY349LlNKByEv_PrOufbVqarnjJ4zpps3iQlGZU1ZU3PBab1_UJ2yVjU1VZQ-vLc-qZ6kdEVpw7Rmj6sT3hrecq5PK7vGcVxGiATmnkxhRPd753YQwWWMfg_Zh5mEgQBJGScSl5RJxORThtkhGYNbEinI9x1CLsoYppDChKRdbZ5WjwYYEz67nc-qz-_ffVp_rDeXHy7Wq03tpBT7GqRxWnWuN13Ta9O20JnOOUTDB-P68lvBjeqhUwKEpMg6Aw2Clq3otZJMnFVvD77XSzdh73DOEUZ7Hf0E8acN4O3xzex3dhu-WcWkkEoUg1e3BjF8XTBlO_nkSnNgxrAky0otrTnlsqAv_0GvwhLn8r1CKakFY6b9S21hROvnIZS67sbUrsqzmWbK0EKd_4cqo8fJuzDj4Mv5keD1kaAwGX_kLSwp2YvLL8csO7AuhpQiDnf9YNTeJMgeEmRLguxNguy-aF7cb-Sd4k9kxC8UKcDb</recordid><startdate>20161207</startdate><enddate>20161207</enddate><creator>Pujol, Vincent</creator><creator>Robles, Jose</creator><creator>Wang, Penghao</creator><creator>Taylor, Jen</creator><creator>Zhang, Peng</creator><creator>Huang, Li</creator><creator>Tabe, Linda</creator><creator>Lagudah, Evans</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-9903-3475</orcidid></search><sort><creationdate>20161207</creationdate><title>Cellular and molecular characterization of a stem rust resistance locus on wheat chromosome 7AL</title><author>Pujol, Vincent ; Robles, Jose ; Wang, Penghao ; Taylor, Jen ; Zhang, Peng ; Huang, Li ; Tabe, Linda ; Lagudah, Evans</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c443z-a49c85bcd9b6d8977ab9bccee92f9cd1183295dab53a340e1b9a6ea8473d85413</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Basidiomycota</topic><topic>Chromosome Mapping</topic><topic>Chromosomes</topic><topic>Chromosomes, Plant - genetics</topic><topic>Cyclopentanes - chemistry</topic><topic>Disease resistance (Plants)</topic><topic>Disease Resistance - genetics</topic><topic>Fluorescein-5-isothiocyanate</topic><topic>Genes, Plant</topic><topic>Genetic aspects</topic><topic>Oxylipins - chemistry</topic><topic>Phenotype</topic><topic>Physiological aspects</topic><topic>Plant Diseases - genetics</topic><topic>Plant Diseases - microbiology</topic><topic>Polymorphism, Single Nucleotide</topic><topic>Salicylic Acid - chemistry</topic><topic>Sequence Analysis, RNA</topic><topic>Signal Transduction</topic><topic>Transcriptome</topic><topic>Triticum - genetics</topic><topic>Triticum - microbiology</topic><topic>Wheat</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pujol, Vincent</creatorcontrib><creatorcontrib>Robles, Jose</creatorcontrib><creatorcontrib>Wang, Penghao</creatorcontrib><creatorcontrib>Taylor, Jen</creatorcontrib><creatorcontrib>Zhang, Peng</creatorcontrib><creatorcontrib>Huang, Li</creatorcontrib><creatorcontrib>Tabe, Linda</creatorcontrib><creatorcontrib>Lagudah, Evans</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BMC research notes</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pujol, Vincent</au><au>Robles, Jose</au><au>Wang, Penghao</au><au>Taylor, Jen</au><au>Zhang, Peng</au><au>Huang, Li</au><au>Tabe, Linda</au><au>Lagudah, Evans</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cellular and molecular characterization of a stem rust resistance locus on wheat chromosome 7AL</atitle><jtitle>BMC research notes</jtitle><addtitle>BMC Res Notes</addtitle><date>2016-12-07</date><risdate>2016</risdate><volume>9</volume><issue>1</issue><spage>502</spage><epage>502</epage><pages>502-502</pages><artnum>502</artnum><issn>1756-0500</issn><eissn>1756-0500</eissn><abstract>Wheat stem rust, caused by Puccinia graminis f. sp. tritici, is a major wheat disease which is mainly controlled through the release of resistant cultivars containing one or several resistance genes. Considerable effort has been put into the discovery of new resistance genes, but knowledge of their mechanisms of action is often lacking. In this study, the mechanism of resistance conferred by a recently discovered stem rust resistance locus on wheat chromosome 7AL was investigated through microscopic observations and RNA-sequencing, using the susceptible line Columbus and the independent, backcrossed, resistant lines Columbus-NS765 and Columbus-NS766.
Microscopic observations of infected leaves revealed that the resistance conferred by the 7AL resistance locus was initiated 2 days post-inoculation, upon the fungus entry into the plant through the stoma. Resistance was manifested by death of guard and epidermal cells adjacent to an infection site. Occasionally, similar observations were made in the susceptible line, suggesting that the resistance response was the same in all genotypes, but enhanced in the resistant lines. Transcriptomic analysis, combined with assignment of genes to wheat chromosomes, revealed a disproportionately high number of differentially expressed genes were located on chromosomes 7AL and 6A. A number of genes annotated as cysteine-rich receptor-like kinases were located on chromosome 7AL. Closer investigation indicated that the encoded proteins were in fact putative receptor-like cytoplasmic kinases. One of the putative RLCK genes contained a SNP marker previously shown to co-segregate with the 7AL resistance locus. The results also indicated the presence of a large introgression on chromosome 6A in both resistant lines, but whether it has any role in the resistance response is unclear.
This study represents the first investigation on the resistance mechanism conferred by the wheat 7AL stem rust resistance locus. The resistance response was associated with pre-haustorial cell death, and the transcriptome analysis suggested putative receptor-like cytoplasmic kinases as candidate resistance genes for further investigation.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>27927228</pmid><doi>10.1186/s13104-016-2320-z</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0001-9903-3475</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1756-0500 |
| ispartof | BMC research notes, 2016-12, Vol.9 (1), p.502-502, Article 502 |
| issn | 1756-0500 1756-0500 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5143453 |
| source | MEDLINE; Springer Nature - Complete Springer Journals; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; PubMed Central Open Access; Springer Nature OA Free Journals |
| subjects | Basidiomycota Chromosome Mapping Chromosomes Chromosomes, Plant - genetics Cyclopentanes - chemistry Disease resistance (Plants) Disease Resistance - genetics Fluorescein-5-isothiocyanate Genes, Plant Genetic aspects Oxylipins - chemistry Phenotype Physiological aspects Plant Diseases - genetics Plant Diseases - microbiology Polymorphism, Single Nucleotide Salicylic Acid - chemistry Sequence Analysis, RNA Signal Transduction Transcriptome Triticum - genetics Triticum - microbiology Wheat |
| title | Cellular and molecular characterization of a stem rust resistance locus on wheat chromosome 7AL |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-31T21%3A29%3A07IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Cellular%20and%20molecular%20characterization%20of%20a%20stem%20rust%20resistance%20locus%20on%20wheat%20chromosome%207AL&rft.jtitle=BMC%20research%20notes&rft.au=Pujol,%20Vincent&rft.date=2016-12-07&rft.volume=9&rft.issue=1&rft.spage=502&rft.epage=502&rft.pages=502-502&rft.artnum=502&rft.issn=1756-0500&rft.eissn=1756-0500&rft_id=info:doi/10.1186/s13104-016-2320-z&rft_dat=%3Cgale_pubme%3EA473181590%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1854831197&rft_id=info:pmid/27927228&rft_galeid=A473181590&rfr_iscdi=true |